FastQCFastQC Report
Fri 27 May 2016
SRR939377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605117
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99230.618210385909563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.4854474782835146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.33611256998711No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42030.2618500707425066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.1236046967292727No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16480.10267164325092812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141200.030.479011
GGTATCA94400.029.3903681
GTACATG98700.027.2015361
TACATGG100350.026.000952
TATCAAC170750.025.2874342
ATCAACG172050.024.7799423
TCAACGC174600.024.595074
CAACGCA174350.024.5534785
ACATGGG102550.024.2630233
AACGCAG178000.023.9946966
GAGTACT88800.023.76873412-13
CATGGGG35950.022.6134074
AGTACTT93250.022.04422212-13
AACGCCG2204.6013156E-822.0112136
ACGCAGA192450.021.927537
CGCAGAG193650.021.8235158
GTATAGG5750.021.6567841
GTACTTT98500.021.61779414-15
CATGGGT13650.021.0200884
GCAGAGT189500.020.7998149
Produced by FastQC (version 0.11.5)