FastQCFastQC Report
Fri 27 May 2016
SRR939377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605117
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128090.7980103631074869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117870.7343389921108554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84570.5268774799594048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62700.3906257300869656No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.19294543637628908No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25410.1583062169299808No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25080.15625029203478624No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.12372929823807237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106550.030.3786431
GTATCAA148800.026.3074171
GTACCGT1452.385878E-522.8672876
GTACATG101550.022.727421
TACATGG102000.022.1576962
GAGTACT104250.022.01485612-13
TATCAAC185700.021.1008532
ATCAACG185050.021.0922413
TCAACGC186350.020.9450994
AGTACTT107850.020.90667312-13
CAACGCA187900.020.7219035
ACATGGG104150.020.602673
GTACTTT111050.020.53883714-15
AACGCAG191200.020.3147096
CTACACT8150.018.598464
ACGCAGA210550.018.4252437
CATGGGG33250.018.377344
ACTTTTT127400.018.34914816-17
CGCAGAG211100.018.3323638
TACTTTT123350.018.14515914-15
Produced by FastQC (version 0.11.5)