##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939377_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1605117 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.882478348930327 31.0 31.0 31.0 28.0 34.0 2 31.13824163596797 31.0 31.0 34.0 28.0 34.0 3 31.10361923772535 31.0 31.0 34.0 28.0 34.0 4 35.05738335585506 35.0 35.0 37.0 32.0 37.0 5 34.68492203372091 35.0 35.0 37.0 32.0 37.0 6 34.66351300247895 35.0 35.0 37.0 30.0 37.0 7 34.535435111583766 35.0 35.0 37.0 30.0 37.0 8 34.555867266996735 35.0 35.0 37.0 30.0 37.0 9 35.86260565429187 37.0 35.0 39.0 30.0 39.0 10-11 35.72403974289725 37.0 35.0 39.0 30.0 39.0 12-13 35.68386852796401 37.0 35.0 39.0 30.0 39.0 14-15 36.45838465357977 38.0 35.0 40.0 30.0 41.0 16-17 36.483414604667445 38.0 35.0 40.0 30.0 41.0 18-19 36.50417726558251 38.0 35.0 40.0 30.0 41.0 20-21 36.433213591283376 38.0 35.0 40.0 30.0 41.0 22-23 36.28255697248238 38.0 34.0 40.0 30.0 41.0 24-25 36.043867207188015 38.0 34.0 40.0 29.0 41.0 26-27 35.643457143622555 38.0 34.0 40.0 27.0 41.0 28-29 35.35004052664074 38.0 34.0 40.0 27.0 41.0 30-31 35.08295750403242 37.5 33.0 40.0 27.0 41.0 32-33 34.80753272191373 37.0 33.0 40.0 26.0 41.0 34-35 34.705965671038314 37.0 33.0 40.0 25.5 41.0 36-37 34.63069358806866 37.0 33.0 40.0 25.0 41.0 38-39 34.562430962976535 37.0 33.0 40.0 25.0 41.0 40-41 34.321227362242126 37.0 33.0 40.0 24.5 41.0 42-43 33.97248082227028 37.0 33.0 40.0 24.0 41.0 44-45 33.7101476091774 37.0 32.5 40.0 23.0 41.0 46-47 33.288936569732925 37.0 32.0 40.0 21.0 41.0 48-49 32.90291330787724 37.0 31.0 40.0 18.5 41.0 50-51 32.609954289936496 36.5 31.0 39.5 17.5 41.0 52-53 32.242420957475375 36.0 31.0 39.0 14.5 40.0 54-55 32.02331294229642 36.0 30.5 39.0 13.5 40.0 56-57 31.631484807649535 35.5 30.0 39.0 10.5 40.0 58-59 31.199435928969663 35.0 29.0 39.0 8.0 40.0 60-61 30.90328181683952 35.0 29.0 38.0 8.0 40.0 62-63 30.5252121807943 35.0 28.0 38.0 7.0 40.0 64-65 30.0907083408873 34.0 28.0 38.0 4.0 40.0 66-67 28.33490362384798 33.0 24.5 37.0 2.0 39.5 68-69 24.634145672869955 29.0 13.0 35.0 2.0 38.0 70-71 24.016800021431457 28.5 10.5 34.5 2.0 38.0 72-73 25.875876649490348 31.0 20.0 35.0 2.0 38.0 74-75 26.496918604687384 31.0 22.0 35.0 2.0 37.5 76-77 26.61976915078465 31.0 23.5 34.5 2.0 36.5 78-79 27.45138267179277 32.5 26.0 35.0 2.0 37.0 80-81 27.304874660227263 32.5 26.0 35.0 2.0 37.0 82-83 26.883233434073652 32.0 25.0 35.0 2.0 36.0 84-85 26.518024231255417 32.0 24.5 35.0 2.0 36.0 86-87 26.059935194755273 32.0 23.5 34.5 2.0 35.5 88-89 25.543186882949964 31.5 21.0 34.0 2.0 35.0 90-91 25.053424142912945 31.0 20.0 34.0 2.0 35.0 92-93 24.458926358639275 31.0 17.5 34.0 2.0 35.0 94-95 23.038114667030506 30.0 4.5 34.0 2.0 35.0 96-97 22.209951984808583 29.0 2.0 34.0 2.0 35.0 98-99 21.8525774133599 29.5 2.0 34.0 2.0 35.0 100-101 20.60789306947718 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3156712130536832 1101 2 0.25529835349608376 1101 3 0.2462468048797426 1101 4 0.281455464173618 1101 5 0.28291979537807777 1101 6 0.5587486949551632 1101 7 0.5781077825774616 1101 8 0.46662670723103616 1101 9 0.7145820947650492 1101 10-11 0.7801488888349155 1101 12-13 0.6140235037487898 1101 14-15 0.6899857861692169 1101 16-17 0.6595999646831032 1101 18-19 0.8510407271457083 1101 20-21 0.7702752913768975 1101 22-23 0.8141661566208 1101 24-25 0.87639437079266 1101 26-27 0.7368384895621105 1101 28-29 0.8363701726749113 1101 30-31 0.9022673366834937 1101 32-33 0.8164253461772475 1101 34-35 0.7726923994549111 1101 36-37 0.7849259142078111 1101 38-39 0.7106704008827407 1101 40-41 1.0080591047972973 1101 42-43 0.8086418251001319 1101 44-45 0.7322911900810851 1101 46-47 0.6964929712769106 1101 48-49 0.6321362292957886 1101 50-51 0.33231289120098495 1101 52-53 0.6367965480565161 1101 54-55 0.5258320470221243 1101 56-57 0.46064955080175807 1101 58-59 0.5257812530232506 1101 60-61 0.20020242486766904 1101 62-63 0.3215054214904107 1101 64-65 0.06751584852966275 1101 66-67 -3.1140637558672744 1101 68-69 -7.744152796236843 1101 70-71 -11.122310371579886 1101 72-73 -11.164342902418651 1101 74-75 -9.639882446160897 1101 76-77 -4.4663065565467015 1101 78-79 -1.5514803995993347 1101 80-81 -1.0205041762696752 1101 82-83 -0.6191510898777572 1101 84-85 -0.7002496002740806 1101 86-87 -0.5273208632871373 1101 88-89 -0.8283184483123556 1101 90-91 -0.7844603795759113 1101 92-93 -1.2442571755134821 1101 94-95 -4.846601473034816 1101 96-97 -1.7817032329385825 1101 98-99 -0.9435923201726553 1101 100-101 -0.8123330170845193 1102 1 0.41636889503110197 1102 2 0.30470729518728135 1102 3 0.454525154127726 1102 4 0.25521487765158213 1102 5 0.5591278083340185 1102 6 0.5486094835887556 1102 7 0.5630682632813304 1102 8 0.5464035394411368 1102 9 0.7437428555591978 1102 10-11 0.6296659380231233 1102 12-13 0.6065510852922245 1102 14-15 0.6617096697296745 1102 16-17 0.7090958163228791 1102 18-19 0.7044541683698569 1102 20-21 0.738190682068911 1102 22-23 0.7568077103163091 1102 24-25 0.8104507760558661 1102 26-27 0.8533344217525567 1102 28-29 0.8441694573283129 1102 30-31 0.6257935064702025 1102 32-33 0.4925797999941466 1102 34-35 0.7909542287468199 1102 36-37 1.0704563263305502 1102 38-39 0.9865904098139779 1102 40-41 1.1143248174800746 1102 42-43 1.209433765509587 1102 44-45 0.9017696879945376 1102 46-47 0.873394709334562 1102 48-49 0.6830371957626795 1102 50-51 0.8092971583805451 1102 52-53 0.8588338020413104 1102 54-55 0.8505748147718926 1102 56-57 0.5470569761616595 1102 58-59 0.40656892565961655 1102 60-61 0.5344214859858525 1102 62-63 0.23678291859694767 1102 64-65 0.45112901305514086 1102 66-67 -2.5748002434900243 1102 68-69 -7.581963428644503 1102 70-71 -10.375100604880103 1102 72-73 -9.057348276703767 1102 74-75 -7.7558012397660185 1102 76-77 -3.6802018321784224 1102 78-79 -0.939473825532982 1102 80-81 -0.26150306127862777 1102 82-83 -0.12259343042626725 1102 84-85 -0.21166236035033492 1102 86-87 -0.2063978937649722 1102 88-89 0.014104488000317161 1102 90-91 -0.40094981118484796 1102 92-93 -0.8272525021606363 1102 94-95 -4.058069629370976 1102 96-97 -0.9620798144523377 1102 98-99 -0.08249646344547301 1102 100-101 0.08912721136852397 1103 1 0.2861247607430144 1103 2 0.31734461716676776 1103 3 0.43824915544829324 1103 4 0.3039893679237764 1103 5 0.5189392858900561 1103 6 0.46529787511435217 1103 7 0.5177901471396638 1103 8 0.4839998782806276 1103 9 0.7154146830657169 1103 10-11 0.6986765651790137 1103 12-13 0.49040572037151264 1103 14-15 0.6942713436632602 1103 16-17 0.6187338270467961 1103 18-19 0.44972659240874435 1103 20-21 0.7505752746851186 1103 22-23 0.783524306211163 1103 24-25 0.9564606120094723 1103 26-27 0.7956345720240776 1103 28-29 0.781372756471086 1103 30-31 0.8061361141363292 1103 32-33 0.8866597285013427 1103 34-35 1.024938501466849 1103 36-37 1.0136730474516682 1103 38-39 1.0440649945750735 1103 40-41 0.8755120762772179 1103 42-43 1.0065088784978329 1103 44-45 0.7343317888358385 1103 46-47 0.829608777935789 1103 48-49 0.8357086418983073 1103 50-51 0.841634278859587 1103 52-53 0.8220820289512929 1103 54-55 0.6541299598635923 1103 56-57 0.6793047306271127 1103 58-59 0.801719965606793 1103 60-61 0.6994957394665775 1103 62-63 0.729473831724075 1103 64-65 0.5615179544378677 1103 66-67 -2.8000986519780895 1103 68-69 -7.416158593772405 1103 70-71 -11.20022583114119 1103 72-73 -8.977875849249617 1103 74-75 -7.295254627136384 1103 76-77 -3.709264997442933 1103 78-79 -0.8603069216953649 1103 80-81 -0.27019049083885704 1103 82-83 -0.02601438419500468 1103 84-85 -0.11175243787677047 1103 86-87 0.026044388312872258 1103 88-89 0.22284343109066995 1103 90-91 0.19046407868434656 1103 92-93 0.43441104333633973 1103 94-95 1.226428782532352 1103 96-97 1.1553694689119212 1103 98-99 0.776957976963903 1103 100-101 0.6135466168853121 1104 1 0.34196252898335544 1104 2 0.24288023948436432 1104 3 0.380432846435415 1104 4 0.34791726491948793 1104 5 0.4833661814551462 1104 6 0.5039636834119179 1104 7 0.4677543817605496 1104 8 0.3241761300688992 1104 9 0.6068530235521266 1104 10-11 0.6672369371389522 1104 12-13 0.680634904920872 1104 14-15 0.8273591834343534 1104 16-17 0.6748266739709763 1104 18-19 0.673682815584705 1104 20-21 0.8431392231829733 1104 22-23 0.7007822461253213 1104 24-25 0.7001681942698426 1104 26-27 0.773359178604899 1104 28-29 0.8604099524653677 1104 30-31 0.9844877593437644 1104 32-33 0.6468185267845996 1104 34-35 0.8103692596928909 1104 36-37 0.9758292706276279 1104 38-39 1.0611891719713569 1104 40-41 1.1402176556763592 1104 42-43 1.2881761174105648 1104 44-45 1.180562260938835 1104 46-47 1.0578983344450847 1104 48-49 1.098409786390441 1104 50-51 0.9851641787165306 1104 52-53 1.1105911848883423 1104 54-55 0.9910133647276815 1104 56-57 1.0007991512279695 1104 58-59 0.8956766179673075 1104 60-61 0.764668700839966 1104 62-63 0.9069990105509724 1104 64-65 0.5205677398455926 1104 66-67 -2.4585986519780896 1104 68-69 -6.931649723958387 1104 70-71 -8.544709092943762 1104 72-73 -7.527167551681661 1104 74-75 -6.561348189368143 1104 76-77 -3.3075475439379254 1104 78-79 -0.3612980518813451 1104 80-81 0.01673354349590994 1104 82-83 0.29511408504676595 1104 84-85 0.2874188067584207 1104 86-87 0.4392204970396243 1104 88-89 0.4616192536943906 1104 90-91 0.21995063090179912 1104 92-93 0.4676023165838359 1104 94-95 1.3416141902576726 1104 96-97 1.361431414548548 1104 98-99 1.1511855878365793 1104 100-101 1.1201666454976156 1105 1 0.471792463907434 1105 2 0.4162607167077894 1105 3 0.5601734104267386 1105 4 0.35638060548347994 1105 5 0.6307018285990367 1105 6 0.7331460406064281 1105 7 0.578347295715723 1105 8 0.6957130787312309 1105 9 0.7420397191414239 1105 10-11 0.9802771395973764 1105 12-13 0.8979390263959246 1105 14-15 1.215061424938341 1105 16-17 1.1050046927706845 1105 18-19 1.1183886724176801 1105 20-21 1.0552416093001042 1105 22-23 1.2769835476437592 1105 24-25 1.1752027568150325 1105 26-27 1.1928356789664392 1105 28-29 1.2963651223858292 1105 30-31 1.13786621198296 1105 32-33 1.3708120191924138 1105 34-35 1.397219440459338 1105 36-37 1.3520748237729663 1105 38-39 1.3528474510747586 1105 40-41 1.4049708010125883 1105 42-43 1.3848848665067308 1105 44-45 1.2680212631080323 1105 46-47 1.2818934175976509 1105 48-49 1.169060400996372 1105 50-51 1.0784282423463196 1105 52-53 1.2566564054234135 1105 54-55 1.2532165462172067 1105 56-57 1.5207787607724406 1105 58-59 1.662922171112644 1105 60-61 1.7278192431393151 1105 62-63 1.731189755308744 1105 64-65 1.7679187159844219 1105 66-67 2.2792126278483735 1105 68-69 -4.4402949878774045 1105 70-71 -4.595851826132481 1105 72-73 -1.103273263901471 1105 74-75 1.5680370600895586 1105 76-77 1.5832362593881761 1105 78-79 1.6110827145684006 1105 80-81 1.6705435218039355 1105 82-83 1.756433969356241 1105 84-85 1.7649029716174205 1105 86-87 1.7395573010887908 1105 88-89 1.6734156383653982 1105 90-91 1.908308403859138 1105 92-93 2.095711933358963 1105 94-95 3.061843257502794 1105 96-97 2.6686645309621397 1105 98-99 2.4453401793044023 1105 100-101 2.2398406874354286 1106 1 0.09277028521604436 1106 2 0.15323591815472426 1106 3 0.17453367746034587 1106 4 0.052187625030292395 1106 5 0.16720293122430974 1106 6 0.28174687824110123 1106 7 0.3178005498122616 1106 8 0.11238074687407362 1106 9 0.273744251622297 1106 10-11 0.3762393378776352 1106 12-13 0.3191590046438648 1106 14-15 0.41253665342512136 1106 16-17 0.26669297129323155 1106 18-19 0.3033071923160051 1106 20-21 0.49205667470651804 1106 22-23 0.5673994206405553 1106 24-25 0.6158586836511901 1106 26-27 0.772930739085048 1106 28-29 0.7168051509879874 1106 30-31 0.5817014250870756 1106 32-33 0.598995812103162 1106 34-35 0.6872010233124612 1106 36-37 0.8043035088547796 1106 38-39 0.6702366327285105 1106 40-41 0.7710520047068243 1106 42-43 0.8704505403099105 1106 44-45 0.6770542276978446 1106 46-47 0.6507101534663207 1106 48-49 0.5756072009795474 1106 50-51 0.5993277982917817 1106 52-53 0.5969837980000676 1106 54-55 0.6536652576178064 1106 56-57 0.8407592620312947 1106 58-59 0.9664666456681381 1106 60-61 0.9888304736931488 1106 62-63 0.891764292176088 1106 64-65 0.8074065210090282 1106 66-67 1.3526368050856235 1106 68-69 -5.255533749812496 1106 70-71 -6.354949536157058 1106 72-73 -2.4644336643317075 1106 74-75 0.22497203223850448 1106 76-77 0.44656510610824185 1106 78-79 0.36475698043629023 1106 80-81 0.5418482249363521 1106 82-83 0.5746989419258064 1106 84-85 0.6912282250409696 1106 86-87 0.4691011987940108 1106 88-89 0.5205983857350205 1106 90-91 0.37467713136348024 1106 92-93 0.39148560374911767 1106 94-95 1.3102169603407745 1106 96-97 1.2056035290453124 1106 98-99 0.994916705103428 1106 100-101 0.736823339865115 1107 1 -0.0072388331051804755 1107 2 -0.06242464133288905 1107 3 0.017774957672646252 1107 4 0.02516312190019221 1107 5 0.03809913643055296 1107 6 0.0315017840551306 1107 7 0.13317246726944632 1107 8 0.08555244486269231 1107 9 0.30593255438829203 1107 10-11 0.19015468212570852 1107 12-13 0.23223672103891602 1107 14-15 0.20634185464132315 1107 16-17 -0.0637941357149927 1107 18-19 0.22652322421127025 1107 20-21 0.33519733896049786 1107 22-23 0.2679386592921773 1107 24-25 0.4488207860216349 1107 26-27 0.3854689024036162 1107 28-29 0.23721509813317 1107 30-31 0.2516653605545116 1107 32-33 0.383828742448614 1107 34-35 0.3067653247704527 1107 36-37 0.19015753396474366 1107 38-39 0.0258264780629176 1107 40-41 0.0992019916581981 1107 42-43 0.14961281812944094 1107 44-45 0.10784444028049478 1107 46-47 -0.2592302315783712 1107 48-49 0.02110778109343414 1107 50-51 0.13695502245469982 1107 52-53 0.21312134001509264 1107 54-55 0.11159300907123182 1107 56-57 0.26109154623364716 1107 58-59 0.1339503977629981 1107 60-61 -0.04680644104425724 1107 62-63 0.26888202228006364 1107 64-65 0.21672528808622715 1107 66-67 1.024177738487289 1107 68-69 -5.160731978971629 1107 70-71 -5.882873451627077 1107 72-73 -2.2426814374175663 1107 74-75 0.13436531800983786 1107 76-77 0.11000221012941935 1107 78-79 0.23826668515989624 1107 80-81 0.2345705469011321 1107 82-83 0.19623720271608036 1107 84-85 0.134739124579081 1107 86-87 0.39775882469758983 1107 88-89 0.37863870129181976 1107 90-91 0.13157938610422093 1107 92-93 0.3372419684945456 1107 94-95 1.1560950075107925 1107 96-97 1.5698598670646255 1107 98-99 1.2611427577192877 1107 100-101 1.3250789421302294 1108 1 0.14192150334233133 1108 2 -0.0531471109429873 1108 3 0.014069313671882355 1108 4 0.006565697967921835 1108 5 0.04762373131269726 1108 6 0.17183376888200286 1108 7 0.17715609116226005 1108 8 0.05763254032530796 1108 9 0.21685416315326478 1108 10-11 0.09450531320732836 1108 12-13 0.3144104034963675 1108 14-15 0.2464429441181224 1108 16-17 0.10122667397097729 1108 18-19 0.028641220143114765 1108 20-21 0.1559232687670118 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82-83 -6.916995005862322 2315 84-85 -5.080290284150671 2315 86-87 -5.329543139324013 2315 88-89 -4.156025190750054 2315 90-91 -4.023823106471937 2315 92-93 -4.860510814729295 2315 94-95 -2.719997930954449 2315 96-97 -1.6886170702999408 2315 98-99 -3.1337396646886724 2315 100-101 -2.346928303997334 2316 1 -0.5606142665967546 2316 2 -0.5491286002946438 2316 3 -1.1260953303601653 2316 4 -0.5181954422628365 2316 5 -0.39026328447486947 2316 6 -2.5338941434757416 2316 7 -0.5948607195285831 2316 8 -0.6063991922147096 2316 9 -0.1651042508493461 2316 10-11 -0.10053175531039926 2316 12-13 -0.7029496254658696 2316 14-15 -1.4403918294569849 2316 16-17 -1.7234510424194482 2316 18-19 -0.47370723966391637 2316 20-21 -4.93054475471758 2316 22-23 -5.028473002493463 2316 24-25 -3.006592763372886 2316 26-27 -2.5449538969015393 2316 28-29 -1.8655642648458652 2316 30-31 -0.4978871946157142 2316 32-33 -2.323590312994405 2316 34-35 -3.0283695985023265 2316 36-37 -5.735313270808831 2316 38-39 -2.864213406297516 2316 40-41 -2.1693116260915986 2316 42-43 -3.503928486641005 2316 44-45 -0.9555393965197254 2316 46-47 -0.7403720154628317 2316 48-49 -0.2797102872743835 2316 50-51 -0.13427744190963153 2316 52-53 -2.706448962441307 2316 54-55 -1.0051339649224076 2316 56-57 -0.19645167750131165 2316 58-59 0.10163904890653441 2316 60-61 -0.9894174870053405 2316 62-63 -0.8392315603514255 2316 64-65 0.15484840282901757 2316 66-67 1.0688930607290956 2316 68-69 3.7303495393933623 2316 70-71 3.822623503741319 2316 72-73 2.0566939584472514 2316 74-75 0.9527600979246778 2316 76-77 -0.2340963468845203 2316 78-79 0.20118739931570673 2316 80-81 -0.4057283349571321 2316 82-83 -0.3416476092442089 2316 84-85 -0.05954693909793107 2316 86-87 -0.49879644587013416 2316 88-89 -0.03027319566104225 2316 90-91 -0.03746226044258094 2316 92-93 0.1402314141897314 2316 94-95 0.45950148307535343 2316 96-97 0.546968062799003 2316 98-99 -0.5116932335262199 2316 100-101 -0.6716506657362693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 30.0 4 73.0 5 158.0 6 436.0 7 1061.0 8 2056.0 9 3365.0 10 5256.0 11 7726.0 12 9947.0 13 12823.0 14 15378.0 15 19481.0 16 23165.0 17 25987.0 18 22656.0 19 18805.0 20 17801.0 21 18065.0 22 19165.0 23 21341.0 24 25657.0 25 31853.0 26 39577.0 27 49251.0 28 59782.0 29 71201.0 30 82002.0 31 95510.0 32 109791.0 33 125750.0 34 145131.0 35 163884.0 36 167364.0 37 136704.0 38 53978.0 39 2902.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.236667555704 21.55774297515099 16.09365302118983 21.111936447955173 2 15.737125523883346 22.52352568040966 38.99137734431178 22.747971451395216 3 19.113497645342985 26.821533881953773 31.15760408742789 22.907364385275343 4 12.669169910978454 18.71701564434244 37.40674355825775 31.207070886421363 5 12.253943017202495 39.54798282491733 34.115478258269185 14.082595899610995 6 29.47112266582436 38.324309069058515 17.372814567411595 14.831753697705524 7 26.532209178520944 34.94125350363868 20.90551654489984 17.62102077294054 8 26.078223581209343 35.28390765283777 19.51614742102912 19.121721344923763 9 26.151800772155546 17.19818555282886 21.100331003908128 35.54968267110746 10-11 24.33993908232235 28.056926691325305 26.841625875247722 20.761508351104624 12-13 25.684607414911188 25.928764071404142 27.827223809853113 20.559404703831557 14-15 22.583618515036598 26.91009440433314 27.365855573145137 23.140431507485122 16-17 20.354274233114502 30.207726872726994 28.05351649806478 21.38448239609373 18-19 20.065298336016976 27.861474822821958 31.896439742332927 20.176787098828136 20-21 21.384162074318017 26.730693211796908 31.912968256801456 19.972176457083616 22-23 20.868011490751144 26.820661671392177 31.873190552464397 20.43813628539228 24-25 20.68690319771082 27.13076367641736 32.139495127146496 20.042837998725325 26-27 20.49759752042302 27.17276826390674 32.19310172962954 20.136532486040696 28-29 20.02869427049257 27.45013552668474 32.366140137707575 20.15503006511512 30-31 20.311717311176515 27.27408095977431 32.170800302047795 20.243401427001377 32-33 20.198183031450217 27.414966283089953 31.94380494223919 20.443045743220637 34-35 20.7679982952894 27.309887539568635 31.824909522920912 20.097204642221058 36-37 20.765091902392783 27.06624861433113 31.81486372502093 20.353795758255156 38-39 20.366511705648076 27.520964641695784 31.94153225554972 20.170991397106423 40-41 20.43478979556384 27.42056263062048 31.992392550862654 20.152255022953025 42-43 20.406767652797473 27.565552788209462 31.403377090802387 20.624302468190677 44-45 20.553287090955298 27.696440459430345 31.1350274320513 20.615245017563062 46-47 20.832041869013402 27.944710151751472 30.505956742753405 20.71729123648172 48-49 20.838746212024358 28.136886634830727 30.18228434186983 20.842082811275088 50-51 20.792334842670257 28.335537416349048 30.093419650649984 20.77870809033071 52-53 21.06485219600124 28.494519739102298 29.7341641690261 20.706463895870368 54-55 20.72919842224271 28.195242165295447 29.92885504121133 21.146704371250504 56-57 20.900347394912107 28.60517265664245 29.49475666775111 20.99972328069434 58-59 21.06247836637845 28.15783890285176 29.646169754647083 21.133512976122713 60-61 20.982992479905345 28.324329510927488 29.9244252138596 20.768252795307564 62-63 20.91195566102565 28.28410138004808 30.020111034341397 20.783831924584867 64-65 20.77523132550613 28.31066572339005 29.805734237109462 21.108368713994356 66-67 20.61763935651069 28.27572785522022 29.992425230833348 21.11420755743574 68-69 20.915038634224555 27.857010016373522 30.30584432964688 20.92210701975504 70-71 20.609381942428918 27.840686654468165 30.599939059919066 20.949992343183848 72-73 20.97912344264656 28.097391234801368 29.595487217041157 21.327998105510922 74-75 20.527183637557737 28.635646749211034 29.502191008519112 21.334978604712113 76-77 20.674079730470297 28.492316664999866 29.367060686242713 21.466542918287125 78-79 20.60175752749875 28.47511685489308 29.413413982212884 21.509711635395288 80-81 20.57468106328644 28.781776077363453 29.132128298628306 21.5114145607218 82-83 20.790322450014546 28.733668635993514 28.947734028111345 21.528274885880595 84-85 20.529905296623237 28.59202787086549 28.981719089636453 21.89634774287482 86-87 20.53636788982195 28.548212553524337 29.111475779556677 21.803943777097036 88-89 20.70543671597808 28.496173147551097 28.853937904373854 21.944452232096964 90-91 20.532633630351974 28.472607288690405 29.133173671795852 21.861585409161766 92-93 20.589093774969356 28.54147035139719 29.077265017436687 21.792170856196762 94-95 20.1689535384014 28.173264801075565 29.379437949727333 22.278343710795703 96-97 20.139816731358472 28.07035237700621 29.358828284708054 22.431002606927265 98-99 20.299327433892994 28.146835499826157 28.868532455022063 22.685304611258783 100-101 20.169620413265957 27.93674499398908 29.145284207961954 22.748350384783013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5189.0 1 3439.5 2 1641.5 3 1691.0 4 1615.0 5 1551.0 6 2193.0 7 3825.5 8 5391.0 9 6072.5 10 5539.5 11 4460.0 12 3973.0 13 3821.5 14 3842.0 15 3935.0 16 4026.0 17 4104.0 18 4310.0 19 4658.5 20 5142.0 21 5778.0 22 6582.5 23 7559.0 24 8649.5 25 10424.0 26 12966.0 27 15574.5 28 17750.5 29 20252.0 30 23530.0 31 27247.0 32 31562.0 33 35884.0 34 39640.0 35 43357.5 36 48151.0 37 51772.5 38 52499.5 39 52300.0 40 51621.5 41 51115.5 42 51210.5 43 51683.5 44 52232.5 45 52945.5 46 53310.5 47 54901.5 48 59806.0 49 59068.5 50 52795.0 51 48425.5 52 45287.5 53 43614.0 54 41465.0 55 37877.5 56 33955.5 57 30280.5 58 27374.5 59 24244.5 60 20521.0 61 16736.5 62 13354.0 63 10344.0 64 7867.5 65 6071.5 66 4585.0 67 3479.5 68 2504.0 69 1698.5 70 1233.0 71 966.0 72 809.5 73 715.0 74 580.5 75 416.0 76 286.5 77 163.5 78 96.0 79 65.0 80 39.0 81 24.5 82 15.5 83 8.0 84 5.5 85 4.5 86 4.0 87 2.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023985790443936485 2 0.017132707459954634 3 0.0 4 0.0 5 6.230075439983502E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8035339479925763E-4 18-19 0.0014017669739962882 20-21 9.345113159975254E-5 22-23 0.0 24-25 0.0 26-27 2.8035339479925763E-4 28-29 0.01663430142475595 30-31 0.004547955071187957 32-33 0.008472902598377564 34-35 0.009500865045974843 36-37 0.10276509438252787 38-39 0.10609818474291906 40-41 0.12157992221127804 42-43 0.10563092908492029 44-45 0.10027306420653448 46-47 0.10217323721572943 48-49 0.10516367342692153 50-51 0.10301429740012721 52-53 0.07912195808779048 54-55 0.0665372056990238 56-57 0.03756735490310052 58-59 0.0165408502931562 60-61 0.009376263537175172 62-63 0.0017444211231953809 64-65 0.0045791054483878746 66-67 0.4391268673872372 68-69 0.8424931017489691 70-71 0.9364114890067203 72-73 0.291910184740427 74-75 0.1585242695703802 76-77 0.01541943671395917 78-79 0.008410601843977728 80-81 0.0011214135791970305 82-83 0.0 84-85 0.0 86-87 3.7380452639901014E-4 88-89 7.476090527980203E-4 90-91 0.0034265414919909267 92-93 0.0015263684827959581 94-95 0.23238181391138463 96-97 0.23714782162297204 98-99 0.013643865213563871 100-101 0.007569541659579957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1605117.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.01441931123691 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98490470621813 39.523106947136085 2 13.350346489279088 13.888210404873242 3 4.57998475594517 7.146757426044152 4 2.0552874666204097 4.276183363756953 5 1.0814775851237541 2.8126214294165424 6 0.6418041788107615 2.002984300342021 7 0.42280540070709083 1.5394384179603915 8 0.2950712787798259 1.2278368976925396 9 0.21498710610059718 1.0064186534903257 >10 1.1879788981755262 11.819704047962619 >50 0.11476552444708203 4.144326291666988 >100 0.06658438442848903 6.247645857466878 >500 0.0023043115732781865 0.7638703160262738 >1k 0.001212795564883256 1.1202884583624242 >5k 2.4255911297665122E-4 0.9290212357848653 >10k+ 2.4255911297665122E-4 1.5515859520176918 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12809 0.7980103631074869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11787 0.7343389921108554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8457 0.5268774799594048 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6270 0.3906257300869656 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3097 0.19294543637628908 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2541 0.1583062169299808 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2508 0.15625029203478624 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1986 0.12372929823807237 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.230075439983502E-5 0.0 5 0.0 0.0 0.0 1.2460150879967005E-4 0.0 6 0.0 0.0 0.0 1.2460150879967005E-4 0.0 7 0.0 0.0 0.0 1.2460150879967005E-4 0.0 8 0.0 0.0 0.0 1.2460150879967005E-4 0.0 9 0.0 0.0 0.0 1.2460150879967005E-4 0.0 10-11 0.0 0.0 0.0 1.2460150879967005E-4 0.0 12-13 0.0 0.0 0.0 1.2460150879967005E-4 0.0 14-15 0.0 0.0 0.0 1.2460150879967005E-4 0.0 16-17 0.0 0.0 0.0 2.1805264039942258E-4 0.0 18-19 0.0 0.0 0.0 2.492030175993401E-4 0.0 20-21 0.0 0.0 0.0 3.426541491990926E-4 0.0 22-23 0.0 0.0 0.0 7.476090527980203E-4 0.0 24-25 0.0 0.0 0.0 0.0013706165967963706 0.0 26-27 0.0 0.0 0.0 0.0023362782899938135 0.0 28-29 0.0 0.0 0.0 0.004797158088787297 0.0 30-31 0.0 0.0 0.0 0.009874669572373852 0.0 32-33 0.0 0.0 0.0 0.018627925565550672 0.0 34-35 0.0 0.0 0.0 0.03333090360391174 0.0 36-37 0.0 0.0 0.0 0.056600235372250125 0.0 38-39 0.0 0.0 0.0 0.08989998859896195 0.0 40-41 0.0 0.0 0.0 0.12868220821285925 0.0 42-43 0.0 0.0 0.0 0.16581345783516094 0.0 44-45 0.0 0.0 0.0 0.21390964023183356 0.0 46-47 0.0 0.0 0.0 0.2725034997448784 0.0 48-49 0.0 0.0 0.0 0.3324991262319196 0.0 50-51 0.0 0.0 0.0 0.40183986587893594 0.0 52-53 0.0 0.0 0.0 0.47211511684194984 0.0 54-55 0.0 0.0 0.0 0.5501779621049432 0.0 56-57 0.0 0.0 0.0 0.6381466273175103 0.0 58-59 0.0 6.230075439983502E-5 0.0 0.7239347661260831 0.0 60-61 0.0 6.230075439983502E-5 0.0 0.8132740479354466 0.0 62-63 0.0 6.230075439983502E-5 0.0 0.9009000589988143 0.0 64-65 0.0 6.230075439983502E-5 0.0 0.9950364988969651 0.0 66-67 0.0 6.230075439983502E-5 0.0 1.0888614350231167 0.0 68-69 0.0 6.230075439983502E-5 0.0 1.2098806504447963 0.0 70-71 0.0 6.230075439983502E-5 0.0 1.36706545379558 0.0 72-73 0.0 6.230075439983502E-5 0.0 1.5394827915971234 0.0 74-75 0.0 6.230075439983502E-5 0.0 1.7357613183338036 0.0 76-77 0.0 6.230075439983502E-5 0.0 1.950200514978036 0.0 78-79 0.0 6.230075439983502E-5 0.0 2.1939833669445905 0.0 80-81 0.0 6.230075439983502E-5 0.0 2.472093934585454 0.0 82-83 0.0 6.230075439983502E-5 0.0 2.753880246735908 0.0 84-85 0.0 6.230075439983502E-5 0.0 3.0333925813507676 0.0 86-87 0.0 6.230075439983502E-5 0.0 3.318761186879212 0.0 88-89 0.0 6.230075439983502E-5 0.0 3.6298600039747884 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10655 0.0 30.378643 1 GTATCAA 14880 0.0 26.307417 1 GTACCGT 145 2.385878E-5 22.867287 6 GTACATG 10155 0.0 22.72742 1 TACATGG 10200 0.0 22.157696 2 GAGTACT 10425 0.0 22.014856 12-13 TATCAAC 18570 0.0 21.100853 2 ATCAACG 18505 0.0 21.092241 3 TCAACGC 18635 0.0 20.945099 4 AGTACTT 10785 0.0 20.906673 12-13 CAACGCA 18790 0.0 20.721903 5 ACATGGG 10415 0.0 20.60267 3 GTACTTT 11105 0.0 20.538837 14-15 AACGCAG 19120 0.0 20.314709 6 CTACACT 815 0.0 18.59846 4 ACGCAGA 21055 0.0 18.425243 7 CATGGGG 3325 0.0 18.37734 4 ACTTTTT 12740 0.0 18.349148 16-17 CGCAGAG 21110 0.0 18.332363 8 TACTTTT 12335 0.0 18.145159 14-15 >>END_MODULE