FastQCFastQC Report
Fri 27 May 2016
SRR939376_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939376_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580574
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.610031545501824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72020.4556572485691907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51660.32684328604671464No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42140.2666120029811954No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19300.12210753814753375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129000.033.4569441
GGTATCA84350.030.866471
GTACATG91800.028.3615111
TATCAAC155400.027.8304792
ATCAACG159650.027.3473553
TCAACGC162000.027.1925744
CAACGCA163300.026.7611275
TACATGG95950.026.250812
AACGCAG166700.026.1368266
ACATGGG98050.024.716183
GAGTACT86300.023.78645112-13
ACGCAGA181050.023.63747
CGCAGAG182100.023.2352528
GCAGAGT178000.022.2745959
CATGGGA60700.022.1489834
AGTACTT90350.022.1090212-13
GTACTTT94550.022.03662914-15
CATGGGG31350.021.4424784
TACGTTG1150.003320519321.0647475
ATGGGAT26550.020.255485
Produced by FastQC (version 0.11.5)