FastQCFastQC Report
Fri 27 May 2016
SRR939376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939376_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1580574
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124180.7856639423399348No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110690.7003152019456222No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83890.5307565479376479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60540.38302540722547634No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28410.1797448268793489No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25430.16089091684413384No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23130.1463392413135987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19330.12229734261097551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100600.032.357671
GTATCAA142100.027.7496851
GTACATG102500.024.9989071
TACATGG104650.023.8928242
ACATGGG104900.022.8357733
TATCAAC177950.022.0764082
ATCAACG178800.021.9400623
TCAACGC180950.021.633114
CAACGCA182650.021.4168425
GAGTACT102050.021.3443912-13
GTATAGG5400.021.0896131
AACGCAG186550.020.9945226
AGTACTT105000.020.8575812-13
CTACACG4800.020.7587724
GTACTTT106950.020.25567214-15
CATGGGA65500.019.7762974
CATGGGG33850.019.6242454
ACGCAGA205450.018.9708547
CTGGTCG4250.018.9662369
CGCAGAG207300.018.7558048
Produced by FastQC (version 0.11.5)