##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939376_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1580574 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.780511383839034 31.0 30.0 31.0 28.0 34.0 2 31.026912374871408 31.0 31.0 34.0 28.0 34.0 3 31.004946936998838 31.0 31.0 34.0 27.0 34.0 4 34.94071457584396 35.0 35.0 37.0 32.0 37.0 5 34.51546590036278 35.0 35.0 37.0 30.0 37.0 6 34.59342175690603 35.0 35.0 37.0 30.0 37.0 7 34.42901439603587 35.0 35.0 37.0 30.0 37.0 8 34.33537056790761 35.0 35.0 37.0 30.0 37.0 9 35.79419312224546 37.0 35.0 39.0 30.0 39.0 10-11 35.58652457904533 37.0 35.0 39.0 30.0 39.0 12-13 35.56973099646078 37.0 35.0 39.0 30.0 39.0 14-15 36.30805137880289 38.0 34.5 40.0 30.0 41.0 16-17 36.23946173984894 38.0 34.0 40.0 30.0 41.0 18-19 36.293303888334236 38.0 34.0 40.0 30.0 41.0 20-21 36.198793602830364 38.0 34.0 40.0 29.5 41.0 22-23 36.03605019442304 38.0 34.0 40.0 29.0 41.0 24-25 35.85794970687864 38.0 34.0 40.0 29.0 41.0 26-27 35.373126155434676 38.0 34.0 40.0 27.0 41.0 28-29 35.1336039944982 37.5 33.0 40.0 27.0 41.0 30-31 34.78943693873238 37.0 33.0 39.5 26.0 40.5 32-33 34.476299116650026 37.0 33.0 39.0 25.0 41.0 34-35 34.639415490828014 37.0 33.0 40.0 25.0 41.0 36-37 34.431221505604924 37.0 33.0 40.0 25.0 41.0 38-39 34.221841875166874 37.0 33.0 40.0 24.0 41.0 40-41 34.03604196956296 37.0 33.0 40.0 24.0 41.0 42-43 33.81041570973583 37.0 33.0 40.0 24.0 41.0 44-45 33.35068209397346 37.0 31.5 40.0 22.5 41.0 46-47 32.8409748610315 36.5 31.0 39.0 19.0 40.5 48-49 32.37774726143793 36.0 30.5 39.0 16.0 40.0 50-51 32.099253182704516 36.0 30.0 39.0 15.0 40.0 52-53 31.97322460068304 36.0 30.0 39.0 14.0 40.0 54-55 31.628610871746595 35.5 30.0 39.0 11.0 40.0 56-57 31.13706666059292 35.0 29.0 39.0 8.5 40.0 58-59 30.680657786348505 35.0 28.0 38.0 8.0 40.0 60-61 30.313182426131267 34.0 28.0 38.0 7.0 40.0 62-63 30.134911114569768 34.0 28.0 38.0 4.5 40.0 64-65 29.673110528200514 34.0 27.5 38.0 2.0 40.0 66-67 28.77594025967781 33.5 26.0 37.0 2.0 39.5 68-69 25.33441490243418 30.5 15.0 35.5 2.0 39.0 70-71 23.99412333747107 29.0 10.5 35.0 2.0 38.0 72-73 25.013974669961673 30.0 16.0 35.0 2.0 37.5 74-75 25.756990814729335 30.5 20.5 35.0 2.0 37.0 76-77 25.474243850651725 30.5 21.0 34.0 2.0 36.0 78-79 26.60142422942551 31.5 24.0 35.0 2.0 37.0 80-81 26.601187606527755 32.0 24.0 35.0 2.0 36.0 82-83 26.247846668362254 32.0 23.5 35.0 2.0 36.0 84-85 25.86519137984049 31.5 22.5 34.0 2.0 36.0 86-87 25.402950447116048 31.0 20.5 34.0 2.0 35.0 88-89 24.882261444260124 31.0 19.5 34.0 2.0 35.0 90-91 24.25400297613399 31.0 17.5 34.0 2.0 35.0 92-93 23.659515467165726 30.0 9.0 34.0 2.0 35.0 94-95 22.77710755712798 30.0 2.0 34.0 2.0 35.0 96-97 22.069390930130446 29.0 2.0 34.0 2.0 35.0 98-99 21.2055917660293 29.0 2.0 34.0 2.0 35.0 100-101 19.811679174780807 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20331219327961492 1101 2 0.1846602960659851 1101 3 0.16647134990608592 1101 4 0.13502290532387917 1101 5 0.48145910573203565 1101 6 0.4042086589011191 1101 7 0.4395408511526995 1101 8 0.5887497835437046 1101 9 0.5953475625566114 1101 10-11 0.6236309078931228 1101 12-13 0.5494669692301315 1101 14-15 0.5456381048669101 1101 16-17 0.6529059102556829 1101 18-19 0.44341141116775873 1101 20-21 0.5683416022927119 1101 22-23 0.5966009671221286 1101 24-25 0.5180976088699083 1101 26-27 0.5169299972933104 1101 28-29 0.4650224229225728 1101 30-31 0.18482446449646517 1101 32-33 0.6505180381673057 1101 34-35 0.5826605712857074 1101 36-37 0.610063918330205 1101 38-39 0.9104763784894843 1101 40-41 0.748869892586697 1101 42-43 0.3877114388834073 1101 44-45 0.7401138021894553 1101 46-47 0.6505215836002662 1101 48-49 0.6080028338444166 1101 50-51 0.32412498665683387 1101 52-53 0.17870011992314971 1101 54-55 0.1485517896877866 1101 56-57 0.1801796073219002 1101 58-59 0.31906703625828925 1101 60-61 0.2524879890138152 1101 62-63 -0.28453695588918393 1101 64-65 -0.5076598117467626 1101 66-67 -0.37486600480542975 1101 68-69 -7.020837044357702 1101 70-71 -7.591014136118034 1101 72-73 -5.323005977766968 1101 74-75 -3.326700248663453 1101 76-77 -0.6258427358677991 1101 78-79 -0.22912785880953024 1101 80-81 -0.44312150211516865 1101 82-83 -0.6263603680288696 1101 84-85 -0.6435195114997079 1101 86-87 -0.5582300031399079 1101 88-89 -0.5771248850117843 1101 90-91 -0.887886861447047 1101 92-93 -0.9451548862731087 1101 94-95 -1.0259188344872783 1101 96-97 -1.1445748802045586 1101 98-99 -1.0812801896914834 1101 100-101 -1.0221086029385198 1102 1 0.3769345950783354 1102 2 0.3319555246058101 1102 3 0.4225707676303969 1102 4 0.41792093498076355 1102 5 0.4587802576426441 1102 6 0.44902037268430917 1102 7 0.6310790738058714 1102 8 0.7019957999876496 1102 9 0.6552717927487706 1102 10-11 0.6865659561350057 1102 12-13 0.582730306296412 1102 14-15 0.917289789396726 1102 16-17 0.7191744155907358 1102 18-19 0.592641178807213 1102 20-21 0.6154106381258018 1102 22-23 0.5713788541731333 1102 24-25 0.6532561696983024 1102 26-27 0.9506165930369477 1102 28-29 0.7050334922335537 1102 30-31 0.5869603579893194 1102 32-33 1.030160003807545 1102 34-35 0.663906464545434 1102 36-37 0.8639661670466197 1102 38-39 1.066072439012082 1102 40-41 0.973333990862379 1102 42-43 0.8251899787819994 1102 44-45 0.789783953415359 1102 46-47 0.8533242548558277 1102 48-49 0.9773370432823469 1102 50-51 1.0085363689938873 1102 52-53 0.6113402254241009 1102 54-55 0.3273742636701584 1102 56-57 0.5025063730367414 1102 58-59 0.5277278654895596 1102 60-61 0.5533175253662606 1102 62-63 0.3629751401968129 1102 64-65 0.448011713501252 1102 66-67 0.28201615078026876 1102 68-69 -6.353524955697299 1102 70-71 -7.238182726674694 1102 72-73 -0.9201424914981224 1102 74-75 0.5198608537317035 1102 76-77 0.7677407765575452 1102 78-79 0.5205632452463078 1102 80-81 0.4423682602112393 1102 82-83 0.3129719792550745 1102 84-85 0.0457493009324601 1102 86-87 0.009288188838095124 1102 88-89 -0.13510925155276254 1102 90-91 -0.020505898992617233 1102 92-93 0.042082451439597435 1102 94-95 0.3570506880673818 1102 96-97 -0.0510495636887498 1102 98-99 -0.15343117325128475 1102 100-101 -0.24356504911407129 1103 1 0.41804497181329836 1103 2 0.21306899144300573 1103 3 0.46193380600202616 1103 4 0.338076412902268 1103 5 0.4871674245625286 1103 6 0.5704270558887501 1103 7 0.4449865366888801 1103 8 0.46993827830686286 1103 9 0.6524054368090546 1103 10-11 0.5557101408773377 1103 12-13 0.48028841023257485 1103 14-15 0.6685678900138896 1103 16-17 0.6959761883938356 1103 18-19 0.6924763733548929 1103 20-21 0.6273091053778685 1103 22-23 0.6927484847628591 1103 24-25 0.7000882426704962 1103 26-27 0.7349868273410962 1103 28-29 0.5452837905142616 1103 30-31 0.6261085198076657 1103 32-33 0.6526602398570205 1103 34-35 0.5560206953245697 1103 36-37 0.6769610483495043 1103 38-39 0.8036795554030718 1103 40-41 0.6442579886535285 1103 42-43 0.5481724710639 1103 44-45 0.8790800395719884 1103 46-47 0.8284050123886715 1103 48-49 0.825336631268712 1103 50-51 0.5625374791417244 1103 52-53 0.4361946615805863 1103 54-55 0.3803652251211318 1103 56-57 0.6043355418850744 1103 58-59 0.7100220088874707 1103 60-61 0.48287592686781977 1103 62-63 0.18975187744942446 1103 64-65 0.2741931768935544 1103 66-67 -0.0918264199558898 1103 68-69 -6.65609002666951 1103 70-71 -7.6216900213188055 1103 72-73 -1.1943979342267959 1103 74-75 0.29637192802863765 1103 76-77 0.5140601214558735 1103 78-79 0.3287812004569908 1103 80-81 0.11026267027352787 1103 82-83 -0.06835321680905082 1103 84-85 -0.04591643584233296 1103 86-87 -0.0380104037347877 1103 88-89 0.09682695500340444 1103 90-91 -0.09711225144166491 1103 92-93 -0.10482801857067514 1103 94-95 0.08985498445396445 1103 96-97 0.2280875252632768 1103 98-99 0.5419897530354731 1103 100-101 0.604526526637784 1104 1 0.32047498550800313 1104 2 0.2815992573171364 1104 3 0.27888078462612853 1104 4 0.2142412589164735 1104 5 0.5021760090860639 1104 6 0.41238206893201124 1104 7 0.620709272502701 1104 8 0.6001259797102065 1104 9 0.5658954437287846 1104 10-11 0.5273392315548051 1104 12-13 0.48796019480011665 1104 14-15 0.6246091941665952 1104 16-17 0.5109313548872692 1104 18-19 0.5686997041500277 1104 20-21 0.5983543615962148 1104 22-23 0.5569518901984765 1104 24-25 0.626363109559712 1104 26-27 0.9489969724238847 1104 28-29 0.8344881369178765 1104 30-31 0.7558529528961557 1104 32-33 0.807009104470545 1104 34-35 0.5753415190913955 1104 36-37 0.7318008087356205 1104 38-39 0.7966318497726093 1104 40-41 0.8813939426068913 1104 42-43 0.878790556758581 1104 44-45 0.8411423808741176 1104 46-47 0.7857431152234398 1104 48-49 0.6721377743277017 1104 50-51 0.6679269096099816 1104 52-53 0.5016967476342842 1104 54-55 0.4932929674638231 1104 56-57 0.5492026337727758 1104 58-59 0.8162600477364172 1104 60-61 0.7709500983092923 1104 62-63 0.5334705540720144 1104 64-65 0.4124917330143347 1104 66-67 0.3764305309024216 1104 68-69 -6.169698740322808 1104 70-71 -4.016747912566064 1104 72-73 0.5072364548243904 1104 74-75 1.1743461831438822 1104 76-77 1.3267132459039601 1104 78-79 0.9679987204127549 1104 80-81 0.7414066847036338 1104 82-83 0.5911087232064105 1104 84-85 0.6194517432253299 1104 86-87 0.46914963899963524 1104 88-89 0.6684340415058685 1104 90-91 0.6425072790008208 1104 92-93 0.6527466291453798 1104 94-95 0.8648640322232879 1104 96-97 0.6467307072986053 1104 98-99 0.8229538254292095 1104 100-101 1.113507270089368 1105 1 0.09726022856222016 1105 2 0.1970519822296808 1105 3 0.16069677230574442 1105 4 0.18040615969693619 1105 5 0.389441762556217 1105 6 0.22814885216475744 1105 7 0.2891847197956423 1105 8 0.3524587344630348 1105 9 0.41019166192341316 1105 10-11 0.44128452397121265 1105 12-13 0.30964863186777336 1105 14-15 0.31610026550515613 1105 16-17 0.208423839962677 1105 18-19 0.3838525733420113 1105 20-21 0.4745257795612261 1105 22-23 0.4759067864186832 1105 24-25 0.3998240844332699 1105 26-27 0.478431193039178 1105 28-29 0.496366194998501 1105 30-31 0.36576446981089106 1105 32-33 0.46540426408225954 1105 34-35 0.3005957871356628 1105 36-37 0.41877536443738705 1105 38-39 0.5819605939959445 1105 40-41 0.5960072316146707 1105 42-43 0.5759992863464163 1105 44-45 0.5620740476900181 1105 46-47 0.7595434699622814 1105 48-49 0.8225468625682453 1105 50-51 0.578235565605226 1105 52-53 0.39965062446957234 1105 54-55 0.5242509283619015 1105 56-57 0.5616063362926518 1105 58-59 0.5744025256067538 1105 60-61 0.9255568108314804 1105 62-63 0.4543675594286789 1105 64-65 0.34929787375587296 1105 66-67 0.5003519190686596 1105 68-69 -3.000974376904672 1105 70-71 -3.1117744230970388 1105 72-73 0.4431544142043613 1105 74-75 0.7877932282025917 1105 76-77 0.8317941592111708 1105 78-79 0.6950945823267212 1105 80-81 0.5656489211594256 1105 82-83 0.596027299910233 1105 84-85 0.6064513239669616 1105 86-87 0.5770756087644067 1105 88-89 0.5560241716775849 1105 90-91 0.46165007057366836 1105 92-93 0.4996787786717718 1105 94-95 0.4453346571554633 1105 96-97 0.7140581457129898 1105 98-99 0.6262530092689644 1105 100-101 0.6310003363149939 1106 1 0.1696804168446313 1106 2 0.24894051796772487 1106 3 0.16363322531241664 1106 4 0.1867460759710653 1106 5 0.2399380029556255 1106 6 0.33060552772366236 1106 7 0.3753464330177394 1106 8 0.45548993343628297 1106 9 0.48090843867907296 1106 10-11 0.6192087137588018 1106 12-13 0.3518559441061342 1106 14-15 0.5714204867134782 1106 16-17 0.6042452740537527 1106 18-19 0.37592179837375994 1106 20-21 0.3962192461212837 1106 22-23 0.44502531295091785 1106 24-25 0.38204913634196913 1106 26-27 0.6906067217015135 1106 28-29 0.5444800111106929 1106 30-31 0.6267056130788902 1106 32-33 0.6822885328920236 1106 34-35 0.5101002858891874 1106 36-37 0.4873166797264261 1106 38-39 0.6917147248528934 1106 40-41 0.6653481759833326 1106 42-43 0.6382006853613262 1106 44-45 0.6680551388141396 1106 46-47 0.7415131781312567 1106 48-49 0.7332202308881435 1106 50-51 0.6280914307837335 1106 52-53 0.49237064005898645 1106 54-55 0.5668748540703135 1106 56-57 0.852499972185921 1106 58-59 1.155087787569272 1106 60-61 1.2405233137645766 1106 62-63 0.7009506570170245 1106 64-65 0.9581011851609986 1106 66-67 0.941249922683788 1106 68-69 -2.788802208028386 1106 70-71 -3.096210214225863 1106 72-73 1.0261394497172986 1106 74-75 1.7592619250759753 1106 76-77 1.5318761379907642 1106 78-79 1.1343748489593395 1106 80-81 0.9475809997246643 1106 82-83 0.912490587528076 1106 84-85 0.8617194684915184 1106 86-87 0.7371610145257677 1106 88-89 0.7024402878993072 1106 90-91 0.8293517753291084 1106 92-93 0.9583769287735748 1106 94-95 1.0937229677878406 1106 96-97 1.1266062746481396 1106 98-99 1.2178894938576263 1106 100-101 1.0697228784635122 1107 1 0.06249939480386857 1107 2 0.0634632312400285 1107 3 0.07490525305519924 1107 4 -0.07923905115769259 1107 5 0.05305838138298924 1107 6 0.0878561916384939 1107 7 -0.009645898679970344 1107 8 0.10381369790297867 1107 9 0.23353028412159915 1107 10-11 0.08924485941430049 1107 12-13 0.07657257061642042 1107 14-15 -0.13775894435515568 1107 16-17 -0.04847462347951392 1107 18-19 0.03430818321544393 1107 20-21 0.1497487447685586 1107 22-23 0.06723280152378663 1107 24-25 -0.05467811804556533 1107 26-27 0.3051626102470024 1107 28-29 0.15629738401550242 1107 30-31 0.08409929418820639 1107 32-33 0.2588603711132791 1107 34-35 0.2510470878812683 1107 36-37 0.40015462621303755 1107 38-39 0.5220318811195597 1107 40-41 0.2283384569055258 1107 42-43 -0.18169507943679264 1107 44-45 0.005702294730383528 1107 46-47 0.22468728871135113 1107 48-49 0.05981966058784849 1107 50-51 0.013513108810663255 1107 52-53 -0.3445106681144132 1107 54-55 -0.1740668717447349 1107 56-57 0.17319891919030184 1107 58-59 0.3531841229296937 1107 60-61 0.33662991872239445 1107 62-63 0.13658948079802613 1107 64-65 -0.06241099127774419 1107 66-67 0.08190505736786591 1107 68-69 -2.694541202794685 1107 70-71 -2.5597714504419926 1107 72-73 0.8014514790451557 1107 74-75 0.7940430714339506 1107 76-77 0.4903179677855647 1107 78-79 0.2906644451871152 1107 80-81 0.1245275253107252 1107 82-83 0.055466026363582444 1107 84-85 0.250610140410334 1107 86-87 0.21535343045527 1107 88-89 0.2964014711921976 1107 90-91 0.0010113806514304713 1107 92-93 0.1280766385144929 1107 94-95 0.6197578611013412 1107 96-97 0.7559174636230743 1107 98-99 0.6994379178029035 1107 100-101 0.848867748953861 1108 1 -0.03616562752077357 1108 2 -0.019469980406455534 1108 3 0.08392893767390674 1108 4 0.09097596895110627 1108 5 0.07757013367361765 1108 6 -0.12307637464298438 1108 7 0.17459126880471132 1108 8 0.19723686069006874 1108 9 0.26806354396271814 1108 10-11 0.36649802807338716 1108 12-13 0.20594834616234436 1108 14-15 0.1519723774520969 1108 16-17 0.4039376705853428 1108 18-19 0.3083346999616907 1108 20-21 0.2322896825143843 1108 22-23 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0.2811646715664864 2311 7 -3.2399593469449215 2311 8 -0.9304499826044719 2311 9 -3.5596042336895195 2311 10-11 -1.6810989948618769 2311 12-13 -0.9226123390709589 2311 14-15 -2.0115243319592793 2311 16-17 -0.05073199950843588 2311 18-19 0.400098695555414 2311 20-21 -0.8616262591871262 2311 22-23 0.052423386296695185 2311 24-25 0.16598412470322188 2311 26-27 0.6316373765101346 2311 28-29 0.4502619358279105 2311 30-31 0.5668522996277048 2311 32-33 0.8237900390821054 2311 34-35 0.4519491592523295 2311 36-37 0.5075828261842119 2311 38-39 0.6413800901624285 2311 40-41 0.48332063875439246 2311 42-43 0.5045463543134403 2311 44-45 0.8262345273877969 2311 46-47 0.7666911406668717 2311 48-49 0.8855186736011618 2311 50-51 0.4374686328770352 2311 52-53 0.5277585925421988 2311 54-55 0.8733164883700368 2311 56-57 0.6936359517605126 2311 58-59 0.9188987740736749 2311 60-61 1.311435518913676 2311 62-63 0.9646654193495827 2311 64-65 1.107947059502667 2311 66-67 1.4452861196707367 2311 68-69 3.493130649624959 2311 70-71 4.887981678063181 2311 72-73 3.87449996516159 2311 74-75 2.795420088392401 2311 76-77 2.5757839999021925 2311 78-79 1.940908950304891 2311 80-81 1.6133731605229755 2311 82-83 1.4485529441311016 2311 84-85 1.4837406937487536 2311 86-87 1.418704508686357 2311 88-89 1.489788625245808 2311 90-91 1.5283844823634496 2311 92-93 1.5354466460039191 2311 94-95 1.956324451936112 2311 96-97 1.8986301298935864 2311 98-99 1.9326530787903806 2311 100-101 2.114328313438513 2312 1 -0.08163627999777034 2312 2 0.0010022211612259468 2312 3 -0.04572317528578651 2312 4 0.02984691420623875 2312 5 -1.3373489650654236 2312 6 -0.13378252763118326 2312 7 -0.02452691132355511 2312 8 0.024545199445221044 2312 9 -2.4987382808661067 2312 10-11 0.035041999012442204 2312 12-13 -1.088295136038603 2312 14-15 -4.4746817609436675 2312 16-17 -1.1303117562511105 2312 18-19 -0.22375519764341334 2312 20-21 0.03385310228697591 2312 22-23 0.11386884513271411 2312 24-25 0.1742244175166121 2312 26-27 0.46679710631160987 2312 28-29 0.2834361047314786 2312 30-31 0.06660095502626717 2312 32-33 0.7168493637824511 2312 34-35 0.3272060346640657 2312 36-37 0.508400771574216 2312 38-39 0.6422011082248247 2312 40-41 0.25022463863640354 2312 42-43 0.03849323886944944 2312 44-45 0.7710020121227643 2312 46-47 0.9448170317418629 2312 48-49 0.8075846401901501 2312 50-51 1.0534202272252813 2312 52-53 0.9458681861628406 2312 54-55 1.0781076449730875 2312 56-57 1.0609831539079444 2312 58-59 0.900534640272312 2312 60-61 1.2154593677676928 2312 62-63 1.5368695332891917 2312 64-65 1.4857554820664092 2312 66-67 1.4685763618464875 2312 68-69 3.9897759059104203 2312 70-71 5.012864301977913 2312 72-73 3.3937723809212024 2312 74-75 2.7478686395659153 2312 76-77 2.561845128875767 2312 78-79 1.7584186353682618 2312 80-81 1.4751121210256173 2312 82-83 1.4310786562536535 2312 84-85 1.6016753908570003 2312 86-87 1.5674882220478104 2312 88-89 1.7198334616813113 2312 90-91 1.6221667454235345 2312 92-93 1.8092408064514984 2312 94-95 2.089426474491983 2312 96-97 2.0338289440878583 2312 98-99 2.221714119270999 2312 100-101 1.9265978630093237 2313 1 -0.2733784677915523 2313 2 -0.22555614632896592 2313 3 -0.13648640871814166 2313 4 0.10561121308188604 2313 5 0.25652285583982604 2313 6 -0.17162707318828296 2313 7 -2.870362281533758 2313 8 -3.15931020109878 2313 9 -1.1758061809400573 2313 10-11 -0.35312155279944335 2313 12-13 -0.2191115162890469 2313 14-15 -0.7590742702506361 2313 16-17 -0.3515867785000708 2313 18-19 -0.03310226892783419 2313 20-21 -2.822241860046475 2313 22-23 -0.7296256835026327 2313 24-25 0.08457613914762163 2313 26-27 -1.3652131971516965 2313 28-29 -1.7097331503356372 2313 30-31 -0.9910915455064497 2313 32-33 -1.0585207210769738 2313 34-35 -1.6733216731287115 2313 36-37 -2.5745370942802097 2313 38-39 -2.3162534976238334 2313 40-41 -1.1655840328184937 2313 42-43 -0.15938151513849874 2313 44-45 -0.43455020086561547 2313 46-47 -0.59375076178776 2313 48-49 -2.343204414573634 2313 50-51 -0.7383952881358979 2313 52-53 -0.38987179553660667 2313 54-55 -0.6215125558316394 2313 56-57 -0.33839826225420566 2313 58-59 -0.07649878957764855 2313 60-61 0.30348253175821327 2313 62-63 0.45074097815563263 2313 64-65 0.25925665405402043 2313 66-67 0.6857218512609933 2313 68-69 1.7135327842869366 2313 70-71 4.143634304108801 2313 72-73 3.178286476109161 2313 74-75 1.5188522317392952 2313 76-77 1.677282148642874 2313 78-79 1.4102768938072003 2313 80-81 1.035067748446366 2313 82-83 1.0611912172032802 2313 84-85 0.8075390893240169 2313 86-87 1.2009964134264948 2313 88-89 0.6562076955022391 2313 90-91 1.03515700601843 2313 92-93 1.1985111658192515 2313 94-95 1.4724589767795528 2313 96-97 1.5429403643864354 2313 98-99 1.4268307539743645 2313 100-101 1.6521854236455802 2314 1 -0.29362223650649355 2314 2 -0.25105486000616395 2314 3 -0.34818441506842035 2314 4 -0.0010433233900357664 2314 5 -0.11546543169399115 2314 6 -3.0485009774039256 2314 7 -0.9831176633035241 2314 8 -0.20452791198643183 2314 9 -0.3014988974007764 2314 10-11 -0.5473523799622413 2314 12-13 -2.495454322822482 2314 14-15 -0.8663005726901218 2314 16-17 -1.9318340416750885 2314 18-19 -0.46258526958455093 2314 20-21 -0.7856000361793107 2314 22-23 -0.341129385822839 2314 24-25 -0.44953611736985266 2314 26-27 -2.2509994807934106 2314 28-29 -1.1666088017813223 2314 30-31 -0.49326985936921375 2314 32-33 0.07245224505688697 2314 34-35 -0.07774638257640731 2314 36-37 -0.3951953782593094 2314 38-39 -0.4499774473277114 2314 40-41 -0.4599582533430251 2314 42-43 -0.7350395882450087 2314 44-45 -3.5817046258763128 2314 46-47 -4.051105094014517 2314 48-49 -3.9907420252064334 2314 50-51 -4.119884892117174 2314 52-53 -1.1460654247743953 2314 54-55 -1.478896410221104 2314 56-57 -1.408044960290681 2314 58-59 -1.8201876741821401 2314 60-61 -1.1085411221215402 2314 62-63 -1.169498573107493 2314 64-65 -1.4142776308167733 2314 66-67 -1.093251741465707 2314 68-69 -1.4749906255831071 2314 70-71 1.1319844972078457 2314 72-73 1.043410860813065 2314 74-75 0.6216155664181962 2314 76-77 0.7329820347716769 2314 78-79 -1.5645045518147818 2314 80-81 -0.9680131001049368 2314 82-83 0.13185612860666396 2314 84-85 0.3695744918195416 2314 86-87 0.46897059057151935 2314 88-89 0.4885172926150929 2314 90-91 0.6999638768499281 2314 92-93 -0.1327458758190474 2314 94-95 0.38458927194093206 2314 96-97 0.5292294285338777 2314 98-99 0.37604510295042815 2314 100-101 0.23622146478381012 2315 1 -0.3308193263812278 2315 2 -0.31919482317374914 2315 3 -0.44462008468115854 2315 4 -0.18341455972440457 2315 5 -0.24022090250547024 2315 6 -0.3175117379906425 2315 7 -1.029457084143786 2315 8 -2.839629149024109 2315 9 -1.0488732204684368 2315 10-11 -1.5798151763602704 2315 12-13 -0.9246219337429977 2315 14-15 -0.7772330136058798 2315 16-17 -0.4028470762855747 2315 18-19 -0.3046539279105289 2315 20-21 -0.8943565906035005 2315 22-23 -0.4269829001403309 2315 24-25 -5.4225825538209165 2315 26-27 -0.988266380332611 2315 28-29 -0.8330143334157896 2315 30-31 -1.2393928832738155 2315 32-33 -0.29126327014103737 2315 34-35 -2.3011288431586863 2315 36-37 -3.9927840143920648 2315 38-39 -1.3334548933359258 2315 40-41 -0.30761852252700095 2315 42-43 -1.88236793591798 2315 44-45 -5.071006880884767 2315 46-47 -3.7407086636848206 2315 48-49 -3.0786149403590386 2315 50-51 -1.643051020884748 2315 52-53 -0.5280258781204381 2315 54-55 -1.429775347759687 2315 56-57 -1.2654792337101313 2315 58-59 -1.0038411421335134 2315 60-61 -2.3618281023586327 2315 62-63 -0.8570179465918031 2315 64-65 -0.17020949866812174 2315 66-67 -0.25408617534319333 2315 68-69 2.4541652635653968 2315 70-71 3.0338693581485536 2315 72-73 -2.9378864007205046 2315 74-75 -2.436608428830578 2315 76-77 -1.126222877432248 2315 78-79 -0.8849628210039775 2315 80-81 -1.2213225470717362 2315 82-83 -2.1735605022434754 2315 84-85 -1.3968187319542942 2315 86-87 -0.796753189778098 2315 88-89 -0.6887429255140596 2315 90-91 -0.6902265628728088 2315 92-93 -1.7068991080325588 2315 94-95 -0.7027929568107538 2315 96-97 -0.9871354676242454 2315 98-99 -2.7785927463909417 2315 100-101 -4.20753291460046 2316 1 -0.5716844288839518 2316 2 -0.5606498247416383 2316 3 -0.9290366589820493 2316 4 -0.47459029065591807 2316 5 -0.32269267273047575 2316 6 -0.5374152429225063 2316 7 -0.6836962851099813 2316 8 -1.0320231767617827 2316 9 -5.161069694113923 2316 10-11 -1.782858434590132 2316 12-13 -0.9737376284733443 2316 14-15 -0.609877484544036 2316 16-17 -0.4870043278526879 2316 18-19 -0.509053953990275 2316 20-21 -3.4872307651328995 2316 22-23 -1.2016546523578882 2316 24-25 -0.7472490279942079 2316 26-27 -0.7347122384650007 2316 28-29 -0.22493318193281198 2316 30-31 -0.1048481611628489 2316 32-33 -1.5246902111418166 2316 34-35 -0.7588695607864295 2316 36-37 -0.6032445548017051 2316 38-39 -5.337218216516781 2316 40-41 -5.145732754928609 2316 42-43 -4.399336848237244 2316 44-45 -2.9800626853363283 2316 46-47 -3.158641284792651 2316 48-49 -3.397487984766016 2316 50-51 -1.6848345030899772 2316 52-53 -1.3854643500955746 2316 54-55 -0.3810329970429578 2316 56-57 -3.8154238918636203 2316 58-59 -1.1734751563345576 2316 60-61 -1.15789029324376 2316 62-63 -0.6233220581882897 2316 64-65 -0.24410988023967306 2316 66-67 -0.14789968651556862 2316 68-69 2.4495101061399644 2316 70-71 3.314841387618671 2316 72-73 2.3966857206496748 2316 74-75 0.9713929310420006 2316 76-77 -1.811697621990433 2316 78-79 -0.2238078296817001 2316 80-81 -0.19433233629148106 2316 82-83 -0.0737053379876329 2316 84-85 0.1745656524860344 2316 86-87 0.15405272101053313 2316 88-89 0.0851266415931704 2316 90-91 0.23455199212286004 2316 92-93 -0.03891603503100072 2316 94-95 0.08490431009373722 2316 96-97 0.08488344640262113 2316 98-99 0.08003212126985915 2316 100-101 -0.0014058813891431043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 22.0 4 74.0 5 190.0 6 459.0 7 1131.0 8 2194.0 9 3641.0 10 5824.0 11 7932.0 12 10607.0 13 13008.0 14 16320.0 15 20623.0 16 24170.0 17 26670.0 18 23697.0 19 19952.0 20 19609.0 21 19779.0 22 20870.0 23 23408.0 24 27591.0 25 33602.0 26 41701.0 27 50585.0 28 60982.0 29 73088.0 30 84468.0 31 96942.0 32 110953.0 33 126977.0 34 143855.0 35 155650.0 36 150512.0 37 116956.0 38 44327.0 39 2199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.03525653600769 21.62612656510442 16.22500547383129 21.113611425056604 2 15.802459292333316 22.61215015959076 39.00614569965551 22.57924484842042 3 19.079394825534347 26.957760148402254 31.21995939437 22.742885631693397 4 12.595781862388167 18.81895945851827 37.46481191867643 31.12044676041713 5 12.25105562915751 39.65749151890389 34.05180649561488 14.039646356323715 6 29.43664769887395 38.410792534863916 17.373182147751386 14.779377618510745 7 26.365991089313123 35.037208001650036 20.970356338899666 17.626444570137178 8 26.11918201868435 35.2377680513535 19.483681244914823 19.159368685047333 9 26.037692635713356 17.24303955398482 21.087528960997716 35.631738849304114 10-11 24.316250010677535 28.10492523983332 26.869557817049323 20.709266932439814 12-13 25.66444215835513 25.99479682697552 27.853615205615174 20.48714580905418 14-15 22.502647772265014 26.92129568118924 27.40884007961665 23.1672164669291 16-17 20.414364221424478 30.20661585786358 28.053463126500255 21.325556794211696 18-19 20.02168203083505 27.896238797169254 31.91706291047257 20.165016261523128 20-21 21.42250220489518 26.745378577655966 31.85829957977292 19.973819637675934 22-23 20.824834300493745 26.88264122102631 31.88029396952496 20.412230508954988 24-25 20.62187187305938 27.188134779820672 32.128380654829414 20.061612692290538 26-27 20.516857664839726 27.17178763217627 32.151659667354735 20.159695035629266 28-29 20.048210913255154 27.481707374941085 32.31744545428663 20.152636257517138 30-31 20.330968893213626 27.287534920122248 32.1227163718946 20.258779814769532 32-33 20.301063227390205 27.36303157415228 31.864048615526723 20.471856582930787 34-35 20.695747628959907 27.325295945031097 31.836472575655 20.142483850353994 36-37 20.738410411505747 27.075512799080027 31.854270834916438 20.33180595449779 38-39 20.38285496253303 27.625593114880235 31.855869719753716 20.13568220283302 40-41 20.51524370263501 27.385666294031214 31.96455499190618 20.134535011427594 42-43 20.35737228754801 27.564372698826606 31.40452431783635 20.673730695789033 44-45 20.572565100769737 27.735676057657965 31.09893088085185 20.59282796072045 46-47 20.81890019056339 27.962003831530435 30.494575164578198 20.724520813327977 48-49 20.86902950214945 28.11601791738993 30.243684227688505 20.771268352772122 50-51 20.84041025179953 28.255131867328238 30.11305446501979 20.791403415852443 52-53 20.954055551159296 28.49948563741394 29.785202184062676 20.761256627364087 54-55 20.637942985190836 28.31386313787177 29.964899842698124 21.08329403423927 56-57 20.959519329014896 28.628756784671083 29.4761900090784 20.935533877235624 58-59 21.087192111212538 28.179412011321226 29.611335148484308 21.12206072898193 60-61 20.970055613805183 28.349119790832866 29.923916214186235 20.756908381175716 62-63 20.90739011378361 28.26150363419177 30.040353194849367 20.79075305717525 64-65 20.800166785776767 28.24845512776101 29.86455615590421 21.08682193055802 66-67 20.5953141397089 28.485498319417196 29.666785803004263 21.25240173786964 68-69 21.268846991811667 27.831176082885335 30.03669004307702 20.86328688222598 70-71 20.92916051335978 27.732946161836917 30.39315401112607 20.94473931367723 72-73 21.08651649539691 27.94722362014469 29.682252664299106 21.284007220159296 74-75 20.706069359221974 28.637060839190497 29.26411464535108 21.392755156236447 76-77 20.165246554325638 28.462411401473364 29.82727249673501 21.545069547465985 78-79 20.616795699673172 28.477674178739456 29.372383878203813 21.533146243383566 80-81 20.567705026473856 28.773793966105494 29.121242577212648 21.537258430208002 82-83 20.834375957936068 28.77064989862684 28.857581855389892 21.537392288047204 84-85 20.537653287021 28.622287177589133 28.916579277445354 21.92348025794451 86-87 20.54435619083532 28.583635022327726 29.090864802416377 21.781143984420574 88-89 20.65409293669596 28.454061365816962 28.846451328808175 22.045394368678906 90-91 20.494317784576605 28.509685058824687 29.058118578185464 21.937878578413244 92-93 20.652502991935773 28.539887889801857 28.993700405283523 21.81390871297885 94-95 20.379357136208533 28.195497053398483 29.069409609587172 22.35573620080581 96-97 20.40587180537192 28.074403290722472 29.01987205980922 22.49985284409639 98-99 20.33879107504044 28.17032507579787 28.715645761757884 22.775238087403807 100-101 20.273973642897776 27.911294246730673 29.064947407918773 22.74978470245278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5029.0 1 3345.5 2 1673.0 3 1734.0 4 1586.5 5 1493.0 6 2135.0 7 3773.5 8 5268.0 9 5906.0 10 5387.5 11 4245.0 12 3789.0 13 3694.0 14 3705.5 15 3727.5 16 3862.5 17 4047.0 18 4274.5 19 4665.0 20 5174.5 21 5781.5 22 6627.5 23 7618.0 24 8687.0 25 10351.0 26 12611.5 27 15042.0 28 17298.5 29 19904.5 30 23198.0 31 26924.5 32 31155.0 33 35302.5 34 39079.0 35 42623.5 36 47392.0 37 51369.0 38 52218.0 39 51670.5 40 50506.0 41 50184.5 42 50267.5 43 50681.5 44 51342.5 45 51920.5 46 52179.5 47 53648.5 48 58872.0 49 58468.0 50 52017.0 51 47510.5 52 44311.5 53 42593.0 54 40935.5 55 37472.5 56 33343.5 57 30024.5 58 27106.5 59 23894.0 60 20318.0 61 16622.5 62 13373.0 63 10433.0 64 7888.0 65 5992.0 66 4545.5 67 3469.0 68 2541.0 69 1760.5 70 1275.0 71 995.5 72 806.0 73 708.5 74 574.0 75 412.5 76 281.0 77 170.5 78 98.5 79 63.5 80 39.0 81 27.0 82 18.5 83 12.0 84 9.0 85 4.5 86 1.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02075195466963268 2 0.017841619563525656 3 3.1634077240293715E-4 4 0.07617485799462727 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009711661712770171 12-13 0.0 14-15 0.0 16-17 5.061452358446994E-4 18-19 1.5817038620146858E-4 20-21 0.0 22-23 1.2653630896117485E-4 24-25 0.0035113825736726024 26-27 0.0038909915005561273 28-29 0.0012020949351311612 30-31 1.5817038620146858E-4 32-33 5.694133903252869E-4 34-35 0.002783798797145847 36-37 0.08844887996386122 38-39 0.09594615626981083 40-41 0.08114140812135338 42-43 0.08557017893499451 44-45 0.0841466454591813 46-47 0.08284964829232924 48-49 0.07683917361667343 50-51 0.07607995576290638 52-53 0.06004147860207747 54-55 0.05118393697479524 56-57 0.028439035439024048 58-59 0.02255509707232942 60-61 0.013349580595403948 62-63 0.001898044634417623 64-65 0.0065482539887408 66-67 0.16133379392549796 68-69 0.7437804240737859 70-71 0.9074551397150656 72-73 0.36799922053633677 74-75 0.05599231671531987 76-77 0.20460921159021975 78-79 0.014488407376054521 80-81 0.0033215781102308405 82-83 0.0017398742482161544 84-85 9.490223172088115E-5 86-87 0.001771508325456448 88-89 2.21438540682056E-4 90-91 0.004586941199842588 92-93 0.004776745663284351 94-95 0.08205879636132189 96-97 0.03903645131452245 98-99 0.013887359908488942 100-101 0.01284343535955925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1580574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.81443487346631 #Duplication Level Percentage of deduplicated Percentage of total 1 76.4233314706029 40.36255062767486 2 13.18982648392019 13.93226463654648 3 4.499978388681106 7.129914466230123 4 2.0201840745815205 4.267795209575982 5 1.0090878624330581 2.664720259603804 6 0.6406454108150443 2.0301195195885735 7 0.408313520447092 1.5095393483526087 8 0.28090671725919664 1.1868743619364046 9 0.19785849419040727 0.9404806100023242 >10 1.149915859233514 11.57502444818635 >50 0.1112797369945386 4.032148792499807 >100 0.06501931269938636 6.171346443966259 >500 0.0019480896757957523 0.6638157103531215 >1k 0.0012175560473723453 1.094334569290848 >5k 2.4351120947446904E-4 0.9287503927763378 >10k+ 2.4351120947446904E-4 1.5103206034160386 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12418 0.7856639423399348 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11069 0.7003152019456222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8389 0.5307565479376479 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6054 0.38302540722547634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2841 0.1797448268793489 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2543 0.16089091684413384 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2313 0.1463392413135987 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1933 0.12229734261097551 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.326815448058744E-5 0.0 5 0.0 0.0 0.0 6.326815448058744E-5 0.0 6 0.0 0.0 0.0 6.326815448058744E-5 0.0 7 0.0 0.0 0.0 6.326815448058744E-5 0.0 8 0.0 0.0 0.0 6.326815448058744E-5 0.0 9 0.0 0.0 0.0 6.326815448058744E-5 0.0 10-11 0.0 0.0 0.0 6.326815448058744E-5 0.0 12-13 0.0 0.0 0.0 6.326815448058744E-5 0.0 14-15 0.0 0.0 0.0 9.490223172088115E-5 0.0 16-17 0.0 0.0 0.0 3.796089268835246E-4 0.0 18-19 0.0 0.0 0.0 4.7451115860440576E-4 0.0 20-21 0.0 0.0 0.0 6.326815448058743E-4 0.0 22-23 0.0 0.0 0.0 0.00110719270341028 0.0 24-25 0.0 0.0 0.0 0.0018031424026967418 0.0 26-27 0.0 0.0 0.0 0.002372555793022029 0.0 28-29 0.0 0.0 0.0 0.004144064118478476 0.0 30-31 0.0 0.0 0.0 0.009553491326568703 0.0 32-33 0.0 0.0 0.0 0.018537569262812116 0.0 34-35 0.0 0.0 0.0 0.03242492917130106 0.0 36-37 0.0 0.0 0.0 0.05475858770294842 0.0 38-39 0.0 0.0 0.0 0.08816417326869859 0.0 40-41 0.0 0.0 0.0 0.12552401848948547 6.326815448058744E-5 42-43 0.0 0.0 0.0 0.1640543245681632 6.326815448058744E-5 44-45 0.0 0.0 0.0 0.21011354103003088 6.326815448058744E-5 46-47 0.0 0.0 0.0 0.2704080922500307 6.326815448058744E-5 48-49 0.0 0.0 0.0 0.3338027830395793 6.326815448058744E-5 50-51 0.0 0.0 0.0 0.4031446803503031 6.326815448058744E-5 52-53 0.0 0.0 0.0 0.47286618658791046 6.326815448058744E-5 54-55 0.0 0.0 0.0 0.547459340720523 6.326815448058744E-5 56-57 0.0 0.0 0.0 0.637173583773996 6.326815448058744E-5 58-59 0.0 0.0 0.0 0.7312849635638698 6.326815448058744E-5 60-61 0.0 0.0 0.0 0.8235932009510469 6.326815448058744E-5 62-63 0.0 0.0 0.0 0.9152371227161777 6.326815448058744E-5 64-65 0.0 0.0 0.0 1.012828251002484 6.326815448058744E-5 66-67 0.0 0.0 0.0 1.117442144436135 6.326815448058744E-5 68-69 0.0 0.0 0.0 1.242333481380815 6.326815448058744E-5 70-71 0.0 6.326815448058744E-5 0.0 1.3958852923052005 6.326815448058744E-5 72-73 0.0 6.326815448058744E-5 0.0 1.5753454124893866 6.326815448058744E-5 74-75 0.0 6.326815448058744E-5 0.0 1.7608792755037093 6.326815448058744E-5 76-77 0.0 6.326815448058744E-5 0.0 1.9744409309529325 6.326815448058744E-5 78-79 0.0 6.326815448058744E-5 0.0 2.2252991634684616 6.326815448058744E-5 80-81 0.0 6.326815448058744E-5 0.0 2.4994400768328466 6.326815448058744E-5 82-83 0.0 6.326815448058744E-5 0.0 2.775289230368208 6.326815448058744E-5 84-85 0.0 6.326815448058744E-5 0.0 3.053985450855196 6.326815448058744E-5 86-87 0.0 6.326815448058744E-5 0.0 3.347612955799602 6.326815448058744E-5 88-89 0.0 6.326815448058744E-5 0.0 3.6707866888864427 6.326815448058744E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10060 0.0 32.35767 1 GTATCAA 14210 0.0 27.749685 1 GTACATG 10250 0.0 24.998907 1 TACATGG 10465 0.0 23.892824 2 ACATGGG 10490 0.0 22.835773 3 TATCAAC 17795 0.0 22.076408 2 ATCAACG 17880 0.0 21.940062 3 TCAACGC 18095 0.0 21.63311 4 CAACGCA 18265 0.0 21.416842 5 GAGTACT 10205 0.0 21.34439 12-13 GTATAGG 540 0.0 21.089613 1 AACGCAG 18655 0.0 20.994522 6 AGTACTT 10500 0.0 20.85758 12-13 CTACACG 480 0.0 20.758772 4 GTACTTT 10695 0.0 20.255672 14-15 CATGGGA 6550 0.0 19.776297 4 CATGGGG 3385 0.0 19.624245 4 ACGCAGA 20545 0.0 18.970854 7 CTGGTCG 425 0.0 18.966236 9 CGCAGAG 20730 0.0 18.755804 8 >>END_MODULE