FastQCFastQC Report
Fri 27 May 2016
SRR939375_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939375_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477938
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60460.40908346628884296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.32890418948562117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33180.224501975049021No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA25160.17023718180329622No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20940.1416838866041742No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19260.13031669799409717No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17000.11502512284006501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG78900.025.4799211
TACATGG83300.024.3383642
ACATGGG84500.022.9193213
GAGTACT56000.022.4198712-13
GTATCAA135300.021.8988061
AGTACTT59200.021.49129912-13
CATGGGA56050.021.1061554
GTACTTT61550.021.006614-15
TATCACG1706.410946E-520.1158032
CATGGGG32600.019.833714
TTATAGG8850.019.5000763
ATGGGAG22350.019.0323495
ACTTTTT75450.018.85787616-17
TATCAAC158100.018.4163132
ATCAACG161900.018.0025423
GGTATCA107300.017.9883671
TACTTTT70750.017.90682214-15
TCAACGC162550.017.7966294
CAACGCA162750.017.7015975
AACGCAG166450.017.3076736
Produced by FastQC (version 0.11.5)