FastQCFastQC Report
Fri 27 May 2016
SRR939375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939375_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477938
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81440.5510380002408761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70240.4752567428403627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50530.34189526218285204No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA31990.21645021645021645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31090.21036065112338947No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28190.19073871840361367No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23100.15629884338855893No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18320.12395648531941124No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17560.11881418571009067No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16090.1088678956762733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.10318430137123479No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA14940.10108678442532773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85900.024.6106971
TACATGG88700.023.6160552
ACATGGG88350.023.0091553
GAGTACT65000.022.08990912-13
CCCTATA2005.507063E-721.326582
CGCAATA804.5592576E-521.0276762-63
AGTACTT68200.020.74075512-13
CATGGGA59050.020.6235374
TAGGTCG1404.9319136E-420.308285
GTACTTT71550.020.2001614-15
GTATAGG3553.6379788E-1220.0283451
GTATCAA146100.019.4987351
TAGGACA5350.019.4858254
GGTATCA118400.019.0962851
CGTATAG1250.00610549318.9601691
AGTACAT65550.018.0749032
ATGGGAG24700.018.0335355
ACTTTTT81400.017.98865716-17
CATGGGG33950.017.7261284
CGCGGAA2700.017.55036582-83
Produced by FastQC (version 0.11.5)