##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939375_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477938 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99195839067674 31.0 31.0 31.0 30.0 34.0 2 31.250842051561026 31.0 31.0 34.0 30.0 34.0 3 31.239503280922474 31.0 31.0 34.0 28.0 34.0 4 35.16968100150345 35.0 35.0 37.0 33.0 37.0 5 34.79123481499224 35.0 35.0 37.0 32.0 37.0 6 34.749482048638036 35.0 35.0 37.0 32.0 37.0 7 34.621984819390256 35.0 35.0 37.0 30.0 37.0 8 34.646186105235806 35.0 35.0 37.0 30.0 37.0 9 35.98381325874293 37.0 35.0 39.0 30.0 39.0 10-11 35.84019695007504 37.0 35.0 39.0 30.0 39.0 12-13 35.796812856831615 37.0 35.0 39.0 30.0 39.0 14-15 36.64070955615188 38.0 35.5 40.0 30.0 41.0 16-17 36.65260010907088 38.0 35.5 40.0 30.5 41.0 18-19 36.613295686287245 38.0 35.0 40.0 30.0 41.0 20-21 36.51652234396842 38.0 35.0 40.0 30.0 41.0 22-23 36.37211642166315 38.0 35.0 40.0 30.0 41.0 24-25 36.13583282925265 38.0 34.0 40.0 29.5 41.0 26-27 35.74130274747655 38.0 34.0 40.0 27.5 41.0 28-29 35.45462495720389 38.0 34.0 40.0 27.0 41.0 30-31 35.245824926350096 38.0 34.0 40.0 27.0 41.0 32-33 35.057003406096875 37.5 33.0 40.0 27.0 41.0 34-35 34.998192752334674 38.0 33.0 40.0 26.0 41.0 36-37 35.00041172227793 38.0 33.0 40.0 25.5 41.0 38-39 34.96695734191827 38.0 33.0 40.0 26.0 41.0 40-41 34.738752911150534 38.0 33.0 40.0 25.0 41.0 42-43 34.42831262204504 37.5 33.0 40.0 24.5 41.0 44-45 34.24659018172616 37.0 33.0 40.0 24.0 41.0 46-47 33.904884710996 37.0 33.0 40.0 23.5 41.0 48-49 33.58659531049341 37.0 32.0 40.0 22.5 41.0 50-51 33.320848371176595 37.0 32.0 40.0 22.0 41.0 52-53 32.924259001392485 36.5 31.0 39.5 20.0 41.0 54-55 32.68543538362232 36.0 31.0 39.0 19.5 40.5 56-57 32.299526434803084 36.0 31.0 39.0 18.0 40.0 58-59 31.84808158393654 35.0 30.0 39.0 15.5 40.0 60-61 31.548674910584882 35.0 29.5 38.5 13.5 40.0 62-63 31.14684005689007 35.0 29.0 38.0 12.5 40.0 64-65 30.687838732071306 34.0 29.0 38.0 8.0 40.0 66-67 28.99962278525892 33.5 25.5 37.0 5.0 39.5 68-69 25.37460028769813 30.0 16.0 35.0 2.0 38.0 70-71 24.772683292533245 29.5 15.0 35.0 2.0 38.0 72-73 26.556720241309176 31.0 21.5 35.0 2.0 37.5 74-75 27.149326629398526 31.5 23.5 35.0 2.0 37.0 76-77 27.175852775962184 31.5 25.0 34.5 2.0 36.0 78-79 28.00645730741073 32.5 26.0 35.0 2.0 37.0 80-81 27.870634289124443 33.0 26.0 35.0 2.0 36.0 82-83 27.477955773516886 32.5 26.0 35.0 2.0 36.0 84-85 27.158080717865026 32.0 25.5 35.0 2.0 35.5 86-87 26.710243257836254 32.0 25.0 34.5 2.0 35.0 88-89 26.21785589111316 32.0 24.0 34.0 2.0 35.0 90-91 25.760988620632258 32.0 23.5 34.0 2.0 35.0 92-93 25.200588590319757 31.0 20.5 34.0 2.0 35.0 94-95 23.8498279359486 31.0 10.0 34.0 2.0 35.0 96-97 23.05990474566592 30.0 4.5 34.0 2.0 35.0 98-99 22.71676890370232 30.0 2.0 34.0 2.0 35.0 100-101 21.45449876787795 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37344620998755573 1101 2 0.2701949573086395 1101 3 0.42390431586825983 1101 4 0.35295767523574995 1101 5 0.29147617957581673 1101 6 0.6297879910928472 1101 7 0.6031323378961488 1101 8 0.6178582375748789 1101 9 0.620893955146741 1101 10-11 0.8211126377527975 1101 12-13 0.7432343006163649 1101 14-15 0.9755901585959919 1101 16-17 0.7632433344697347 1101 18-19 0.85792530756639 1101 20-21 0.8920295219562888 1101 22-23 0.9024325359552989 1101 24-25 0.8797220976157476 1101 26-27 0.7641150077044045 1101 28-29 0.7604016954918222 1101 30-31 0.8284679291026862 1101 32-33 0.8110085113429477 1101 34-35 0.7989200976862207 1101 36-37 0.9367136002621379 1101 38-39 0.696922738195191 1101 40-41 0.9612691037446481 1101 42-43 1.1452000638033084 1101 44-45 0.8025376896392871 1101 46-47 0.7060294758467123 1101 48-49 0.7207701372250384 1101 50-51 0.3678097258040438 1101 52-53 0.6269359731795419 1101 54-55 0.4682138343749216 1101 56-57 0.18235205190253367 1101 58-59 0.4965925110232412 1101 60-61 0.30965327775612295 1101 62-63 0.1118489131363205 1101 64-65 -0.1254586796443533 1101 66-67 -3.2021371954223916 1101 68-69 -7.610721984162701 1101 70-71 -11.103478065291752 1101 72-73 -11.215913940672873 1101 74-75 -9.82410930942407 1101 76-77 -4.739554957517186 1101 78-79 -1.7207585780947383 1101 80-81 -1.0078910442068647 1101 82-83 -0.799503394963736 1101 84-85 -0.9814144387297681 1101 86-87 -0.7578815785401822 1101 88-89 -0.3934802034563418 1101 90-91 -0.5572681959376986 1101 92-93 -1.1484679250287542 1101 94-95 -4.931216730684476 1101 96-97 -1.7432589227089572 1101 98-99 -1.0812907625658887 1101 100-101 -1.2053782317903021 1102 1 0.42685149353040686 1102 2 0.3065326734420495 1102 3 0.4176534626459052 1102 4 0.2518050937261975 1102 5 0.4925733747657901 1102 6 0.4017508120507216 1102 7 0.40810696550116177 1102 8 0.4470288859458549 1102 9 0.6470101650062858 1102 10-11 0.6382102385890533 1102 12-13 0.7216067229097476 1102 14-15 0.59107843580842 1102 16-17 0.6475391676242879 1102 18-19 0.5452730439431122 1102 20-21 0.6761773332215597 1102 22-23 0.5664023699133622 1102 24-25 0.6862947375539648 1102 26-27 0.733897479884476 1102 28-29 0.8222600235270505 1102 30-31 0.5704788425479066 1102 32-33 0.5595105454250344 1102 34-35 0.8005617950038868 1102 36-37 1.1501724759198453 1102 38-39 0.9064815588566191 1102 40-41 0.8903227387644037 1102 42-43 0.7837508924528507 1102 44-45 0.6751121003795575 1102 46-47 0.7668374140324659 1102 48-49 0.7712038667135772 1102 50-51 0.8364197242048661 1102 52-53 0.96620896922839 1102 54-55 0.8078653686149764 1102 56-57 0.5813511977050752 1102 58-59 0.5526422963037341 1102 60-61 0.5403626516956912 1102 62-63 0.5356886282478115 1102 64-65 0.7929668277060316 1102 66-67 -2.272902969797247 1102 68-69 -7.321570877021376 1102 70-71 -11.124393616756173 1102 72-73 -9.158242155322167 1102 74-75 -7.34619557946748 1102 76-77 -3.6903910179116757 1102 78-79 -0.9619131488312611 1102 80-81 -0.2644231195166604 1102 82-83 -0.10532012865305163 1102 84-85 -0.25004861988983507 1102 86-87 -0.05101621020165581 1102 88-89 0.039732866154903235 1102 90-91 -0.11287138026102994 1102 92-93 -0.5434568282626131 1102 94-95 -4.312348717844209 1102 96-97 -0.7858188237936723 1102 98-99 -0.20803942958879773 1102 100-101 0.07762187352171424 1103 1 0.3325770952319118 1103 2 0.2186638224675228 1103 3 0.33097426182820655 1103 4 0.16067555240745435 1103 5 0.39671692663932845 1103 6 0.41589455635102013 1103 7 0.4770357408104644 1103 8 0.3949699007291372 1103 9 0.613043823088077 1103 10-11 0.5440339402083438 1103 12-13 0.44445049614952836 1103 14-15 0.6641142019065285 1103 16-17 0.5764289897200925 1103 18-19 0.6906401432681477 1103 20-21 0.7160249538646468 1103 22-23 0.7826702415488569 1103 24-25 0.943786835794235 1103 26-27 0.619885755435071 1103 28-29 0.754414120387132 1103 30-31 0.8682169980204364 1103 32-33 0.7057175309692596 1103 34-35 0.8322758005404864 1103 36-37 0.4950383978572148 1103 38-39 0.5793822866448224 1103 40-41 0.8019439568710851 1103 42-43 0.7983598567478793 1103 44-45 0.6846777856320045 1103 46-47 0.8017357051696195 1103 48-49 0.7589366606482884 1103 50-51 0.8319093918310259 1103 52-53 0.834818089728671 1103 54-55 0.8983451866320777 1103 56-57 0.844265391593531 1103 58-59 0.7153628937775132 1103 60-61 0.6015990728748513 1103 62-63 0.5650944559111579 1103 64-65 0.436060838743785 1103 66-67 -2.9698728897213016 1103 68-69 -7.512449735319439 1103 70-71 -11.212782019987586 1103 72-73 -9.19171384727031 1103 74-75 -7.231517152884518 1103 76-77 -3.3978734759484936 1103 78-79 -0.5038976497988621 1103 80-81 0.03981509877753808 1103 82-83 0.20206011681558422 1103 84-85 0.10811191609792914 1103 86-87 0.3334639677666864 1103 88-89 0.4368277502819211 1103 90-91 0.2860466531072774 1103 92-93 0.17490057162170558 1103 94-95 1.2875375617126323 1103 96-97 1.4003119984836125 1103 98-99 1.0675373884950936 1103 100-101 1.0538211355839486 1104 1 0.4618861523051443 1104 2 0.23772738401700977 1104 3 0.32424420199658144 1104 4 0.3405873918925266 1104 5 0.5387855781148971 1104 6 0.509799150359953 1104 7 0.531392395971487 1104 8 0.3986697992042849 1104 9 0.4860275323598273 1104 10-11 0.6005681912563787 1104 12-13 0.6158760213439152 1104 14-15 0.6202853809365294 1104 16-17 0.8393996349369246 1104 18-19 0.6637906026386347 1104 20-21 0.8035106003187096 1104 22-23 0.6858985493071259 1104 24-25 0.737430832885714 1104 26-27 0.9872909194047352 1104 28-29 0.9504298687119359 1104 30-31 0.8873664622877513 1104 32-33 0.8061809495249364 1104 34-35 0.8526075674795806 1104 36-37 0.9356511122956732 1104 38-39 0.6033609280363237 1104 40-41 0.7765494410374245 1104 42-43 0.9307912495540194 1104 44-45 0.8852478984040246 1104 46-47 0.7626843676529091 1104 48-49 0.6725662291239658 1104 50-51 0.6805379701448473 1104 52-53 0.8109363739701223 1104 54-55 0.7518002813664353 1104 56-57 0.9050721625521732 1104 58-59 0.8877531140951795 1104 60-61 0.5119012413863437 1104 62-63 0.5402356917623905 1104 64-65 0.34971568390413665 1104 66-67 -2.976615872970651 1104 68-69 -7.178607067287452 1104 70-71 -8.664952070879245 1104 72-73 -8.184083899454311 1104 74-75 -7.071520870267616 1104 76-77 -3.671817813267939 1104 78-79 -0.8182789810857365 1104 80-81 0.03960794399338852 1104 82-83 0.1016223550451052 1104 84-85 0.24868886307732652 1104 86-87 0.5051920529761027 1104 88-89 0.5448420557451428 1104 90-91 0.5376731982634091 1104 92-93 0.550665856508946 1104 94-95 1.40710749317671 1104 96-97 1.6050561851312146 1104 98-99 1.2677245499918222 1104 100-101 1.3418696729001276 1105 1 0.6589116385809071 1105 2 0.4333098372104125 1105 3 0.6399046228285847 1105 4 0.4367079630110524 1105 5 0.6229664719555288 1105 6 0.6712813530758126 1105 7 0.7547016725596407 1105 8 0.5618648372218686 1105 9 0.8199884221868743 1105 10-11 0.8588668392722525 1105 12-13 0.8736406393470872 1105 14-15 1.1153856462792078 1105 16-17 1.1646246559339346 1105 18-19 1.1786988475863467 1105 20-21 1.2429446474263273 1105 22-23 1.3254641528688182 1105 24-25 1.3528573136390776 1105 26-27 1.3801690327216747 1105 28-29 1.3908790187977829 1105 30-31 1.2683593418875816 1105 32-33 1.478610767501344 1105 34-35 1.685306407255709 1105 36-37 1.4956020201838527 1105 38-39 1.296199462928513 1105 40-41 1.5167291605323143 1105 42-43 1.4452461078521068 1105 44-45 1.4400712634248762 1105 46-47 1.3682062082540511 1105 48-49 1.3154846641224438 1105 50-51 1.3627237769129366 1105 52-53 1.6020932903463034 1105 54-55 1.3781113863663208 1105 56-57 1.5209196982842457 1105 58-59 1.5554387239002274 1105 60-61 1.5640408919069948 1105 62-63 1.7095083406553115 1105 64-65 1.7986281133876503 1105 66-67 2.5332668411058883 1105 68-69 -4.574161090908156 1105 70-71 -4.5349798686836635 1105 72-73 -0.9670516123788069 1105 74-75 1.718303015032994 1105 76-77 2.0496745720661167 1105 78-79 1.9774725630302719 1105 80-81 1.7941598344670666 1105 82-83 1.9143961522697843 1105 84-85 1.9214000037535435 1105 86-87 2.0644171705964887 1105 88-89 1.8403295578820078 1105 90-91 1.8556836112912158 1105 92-93 2.0894263271391544 1105 94-95 2.884541845019612 1105 96-97 3.014486070000732 1105 98-99 2.9016863688898695 1105 100-101 2.6549394721464665 1106 1 0.03923563228718763 1106 2 0.0791125452836603 1106 3 0.10083183874313306 1106 4 0.06530561542327717 1106 5 0.2703078915886934 1106 6 0.3123523199598992 1106 7 0.24377482599496858 1106 8 0.21839715902902412 1106 9 0.5551490524454934 1106 10-11 0.40328097650789374 1106 12-13 0.268164104144347 1106 14-15 0.33866115173450595 1106 16-17 0.36667934926273205 1106 18-19 0.484381664691675 1106 20-21 0.27472701909570674 1106 22-23 0.1977331799273898 1106 24-25 0.3883069601712492 1106 26-27 0.3852106584520598 1106 28-29 0.504894738328268 1106 30-31 0.6412610652740725 1106 32-33 0.6843166689735725 1106 34-35 0.7119010753209807 1106 36-37 0.69127822592651 1106 38-39 0.7728356828542005 1106 40-41 0.6723658089038409 1106 42-43 0.6578416771310671 1106 44-45 0.7631593238209646 1106 46-47 0.5970814270938476 1106 48-49 0.47201199970634633 1106 50-51 0.7506661657201619 1106 52-53 0.899409778863216 1106 54-55 0.8294899847574371 1106 56-57 0.8208005829510583 1106 58-59 1.0453578732136002 1106 60-61 0.9379575887372376 1106 62-63 1.0078806157766422 1106 64-65 0.9911570888849752 1106 66-67 1.5152414593827963 1106 68-69 -4.812655657771561 1106 70-71 -5.9139649691098946 1106 72-73 -1.9964180665461804 1106 74-75 0.7740524997899243 1106 76-77 0.9459371207279617 1106 78-79 0.4767575016812593 1106 80-81 0.8220610776987272 1106 82-83 0.9094411390839916 1106 84-85 0.9152848626249543 1106 86-87 0.8503340447146677 1106 88-89 0.8645624182012739 1106 90-91 0.5852210744485653 1106 92-93 0.8588600399179214 1106 94-95 1.898640298921059 1106 96-97 1.8521444287576507 1106 98-99 1.486290573568791 1106 100-101 1.6735595071608316 1107 1 -0.04895067642566886 1107 2 -0.12068405555008965 1107 3 -0.14404875656099492 1107 4 -0.09889920846027422 1107 5 0.0806897112111713 1107 6 0.07410066041810381 1107 7 0.0495740100904456 1107 8 -0.17343394888869312 1107 9 -0.12093008244646342 1107 10-11 0.01380138296773481 1107 12-13 0.07526684303259401 1107 14-15 0.14753670975520095 1107 16-17 -0.08668673769579982 1107 18-19 0.14353535799435235 1107 20-21 0.0871393685945634 1107 22-23 0.11010421877863052 1107 24-25 0.22387173518976766 1107 26-27 0.3232575725774538 1107 28-29 0.10510556110251201 1107 30-31 -0.09031588600012697 1107 32-33 0.06179809649107426 1107 34-35 0.0848717955340419 1107 36-37 0.19524639441856095 1107 38-39 -0.03096528136862986 1107 40-41 -0.32847348190696124 1107 42-43 -0.014699320032583785 1107 44-45 -0.20550401132531704 1107 46-47 -0.22438233916020067 1107 48-49 0.03475610370762894 1107 50-51 0.2138669947025491 1107 52-53 0.17557857401548205 1107 54-55 -0.048150877242690626 1107 56-57 0.09299986336111488 1107 58-59 -0.20389938134384167 1107 60-61 -0.07315166754033342 1107 62-63 -0.16579815508926998 1107 64-65 0.03769214870079196 1107 66-67 0.7042234404014387 1107 68-69 -5.3209158640786285 1107 70-71 -6.0548654335277305 1107 72-73 -2.7148706173825126 1107 74-75 -0.334790286655565 1107 76-77 -0.2743946030030422 1107 78-79 -0.048205053332292636 1107 80-81 -0.13764353963158982 1107 82-83 -0.04746270095994731 1107 84-85 0.02589162141616086 1107 86-87 -0.03815913210490507 1107 88-89 -0.20475177119843124 1107 90-91 -0.08857308928775964 1107 92-93 -0.16360310552248336 1107 94-95 0.7239751113345605 1107 96-97 0.9372691722969968 1107 98-99 0.573998578833784 1107 100-101 0.5635054802189039 1108 1 0.06564649698320935 1108 2 0.010127235717884986 1108 3 0.1036742501407808 1108 4 -0.13515702918609662 1108 5 0.04811051035590452 1108 6 0.1644163238983296 1108 7 0.009057586084828984 1108 8 0.026650314305157963 1108 9 0.03887575790174225 1108 10-11 0.10730830310178874 1108 12-13 0.07143494733409739 1108 14-15 0.16481374541609028 1108 16-17 0.05658476154976455 1108 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2311 66-67 1.6777872379319128 2311 68-69 4.133051474198577 2311 70-71 4.725426176688146 2311 72-73 2.5615908186927676 2311 74-75 1.4762243668890775 2311 76-77 0.9530112674414681 2311 78-79 0.7905624635085751 2311 80-81 0.748590441131789 2311 82-83 0.6202734876413558 2311 84-85 0.5395284363853108 2311 86-87 0.6359149997154496 2311 88-89 0.7228224463751545 2311 90-91 0.7770025692683156 2311 92-93 0.8207015917029707 2311 94-95 1.5845776980458446 2311 96-97 1.6611657251813021 2311 98-99 1.0431157226686203 2311 100-101 0.9322082595261989 2312 1 -0.16980496697173564 2312 2 -0.12713542208765105 2312 3 -0.14126876638042418 2312 4 0.09343399758412829 2312 5 0.1109444709078602 2312 6 0.19839502515543472 2312 7 0.09487977627886579 2312 8 0.07446305618467619 2312 9 -1.4819916721033337 2312 10-11 -0.9200200124825955 2312 12-13 -1.6941985434711455 2312 14-15 -1.809582923652691 2312 16-17 0.019155824084791107 2312 18-19 -1.8265028286839566 2312 20-21 -1.9915262797606843 2312 22-23 -0.6025688487026883 2312 24-25 -0.9446728068428527 2312 26-27 -1.3433039898032106 2312 28-29 -0.016784679473566655 2312 30-31 0.5488805571835513 2312 32-33 0.3701167915977308 2312 34-35 0.6381613051079853 2312 36-37 0.6958246498328933 2312 38-39 0.604693438832129 2312 40-41 0.9394164313547364 2312 42-43 0.6614116395440917 2312 44-45 0.8608622534720212 2312 46-47 1.0024099798622217 2312 48-49 1.005214889371267 2312 50-51 1.0545336250009498 2312 52-53 1.1922530598591408 2312 54-55 1.176469165308184 2312 56-57 1.3547253556965089 2312 58-59 1.4348284567446434 2312 60-61 1.374734972577503 2312 62-63 0.9396445376170703 2312 64-65 1.3683740536705145 2312 66-67 2.380967207790018 2312 68-69 4.8250243464936275 2312 70-71 5.566696401614923 2312 72-73 3.78116996960129 2312 74-75 2.822365351334721 2312 76-77 1.6535771388550096 2312 78-79 1.3078235321240363 2312 80-81 1.6794531817693183 2312 82-83 1.6164312491791613 2312 84-85 1.628322476252233 2312 86-87 1.7756769924927696 2312 88-89 1.7712331723398371 2312 90-91 1.6140361518883886 2312 92-93 1.9675719531213431 2312 94-95 2.73349941442768 2312 96-97 2.92558350321184 2312 98-99 2.7854082127871997 2312 100-101 2.584829466908122 2313 1 -0.35883052445061026 2313 2 -0.29246210300948405 2313 3 -0.20494456314246534 2313 4 -0.2015861972364945 2313 5 -0.09652930976620411 2313 6 -0.10361495346181471 2313 7 -3.092694469385183 2313 8 -0.9113700225920951 2313 9 -0.7724149148145258 2313 10-11 -0.2071092430429573 2313 12-13 -1.4598330681291856 2313 14-15 -0.25310259238975163 2313 16-17 -0.0698659998978286 2313 18-19 0.1571108562768444 2313 20-21 -0.06248103657431159 2313 22-23 -1.4798138678792867 2313 24-25 -2.6413238607070184 2313 26-27 -2.0956452313604217 2313 28-29 -0.6505029023293503 2313 30-31 -0.1750623542647034 2313 32-33 -2.6326466744444303 2313 34-35 -2.898182583837471 2313 36-37 -1.3247282470207864 2313 38-39 -0.755384625180362 2313 40-41 -0.6923036921900589 2313 42-43 -0.891109149241835 2313 44-45 -0.11100517347024663 2313 46-47 -0.2749069939693669 2313 48-49 -0.3231135225351949 2313 50-51 -0.2054018873365635 2313 52-53 0.09961381741946695 2313 54-55 0.18294406603111923 2313 56-57 0.511392361771918 2313 58-59 0.017094010150611183 2313 60-61 0.5431044529427034 2313 62-63 0.27576916851005606 2313 64-65 0.2646324116109824 2313 66-67 1.513660076812183 2313 68-69 4.7979975671432555 2313 70-71 4.66312073043412 2313 72-73 2.9296388967302285 2313 74-75 1.9347822810431694 2313 76-77 1.162310125991418 2313 78-79 0.7348411474381606 2313 80-81 0.9489318499293802 2313 82-83 0.648630278470133 2313 84-85 0.978263758538489 2313 86-87 0.8752123419140787 2313 88-89 1.0309677546973717 2313 90-91 1.0757528813456751 2313 92-93 0.9416883024408307 2313 94-95 1.3403938243655702 2313 96-97 1.4816904848498247 2313 98-99 0.9957805441859016 2313 100-101 0.6673746931240494 2314 1 -0.29207178228202935 2314 2 -0.26312204416122853 2314 3 -0.08439771472676583 2314 4 -0.1809472733188784 2314 5 -1.5747114190958555 2314 6 -2.877539571648729 2314 7 -1.0098024979686215 2314 8 -0.3097807193634452 2314 9 -0.14879955021555702 2314 10-11 0.06746791022272447 2314 12-13 -0.05961288758113881 2314 14-15 -2.629293068580232 2314 16-17 -2.690338787583727 2314 18-19 -0.4396875183903788 2314 20-21 -0.41929662535712 2314 22-23 3.148376592605473E-4 2314 24-25 -2.4854420376120814 2314 26-27 -1.1987517787292603 2314 28-29 -0.18643536695034157 2314 30-31 -0.29601353423228005 2314 32-33 0.011740723221656424 2314 34-35 -0.18593754290390052 2314 36-37 0.3626213796731008 2314 38-39 -1.4399219457832544 2314 40-41 -4.003407797556456 2314 42-43 -5.324036149381957 2314 44-45 -2.6154328041273835 2314 46-47 -2.897155237529084 2314 48-49 -1.113042264103278 2314 50-51 -0.8969379469054104 2314 52-53 -2.141529920445585 2314 54-55 -1.3705988391511283 2314 56-57 -3.73705836782635 2314 58-59 -4.838961955741421 2314 60-61 -2.345744403119266 2314 62-63 -1.3625182271543892 2314 64-65 -0.6320458645754883 2314 66-67 -0.6845994960375563 2314 68-69 1.4606319273186017 2314 70-71 1.4949758516335017 2314 72-73 -0.7659574923087824 2314 74-75 -0.25294865832711366 2314 76-77 -0.383509446057662 2314 78-79 0.12653033256996693 2314 80-81 0.5273721709926562 2314 82-83 0.1397720146938397 2314 84-85 0.12647929527861734 2314 86-87 -0.04787559019024812 2314 88-89 0.09465197575864437 2314 90-91 0.5640993254298188 2314 92-93 0.5457696491451287 2314 94-95 0.5795269134986576 2314 96-97 0.7431829086373334 2314 98-99 0.4444754740283692 2314 100-101 0.7699688203083426 2315 1 -0.4182673211604957 2315 2 -0.27723086217248394 2315 3 -0.3538244397632475 2315 4 -0.21640693560780733 2315 5 -1.092364118699777 2315 6 -0.5532910830099951 2315 7 -0.08635034037012446 2315 8 -0.21940756947810058 2315 9 0.16804014125828104 2315 10-11 -1.6545375098314778 2315 12-13 -1.370815722686416 2315 14-15 -0.4870166458159986 2315 16-17 -1.873961885353168 2315 18-19 -3.1024413228515044 2315 20-21 -2.3147500337373543 2315 22-23 -1.990702464821453 2315 24-25 -1.033209392208711 2315 26-27 -0.4590728127088326 2315 28-29 -0.580496238328287 2315 30-31 -3.2870579369840023 2315 32-33 -2.852810037670565 2315 34-35 -1.6117432548071804 2315 36-37 -4.834981288661275 2315 38-39 -2.3518919270215335 2315 40-41 -1.8909229111691346 2315 42-43 -1.9302108418981092 2315 44-45 -1.818618128288314 2315 46-47 -1.5917893838705481 2315 48-49 -4.813682247426193 2315 50-51 -4.036919185179329 2315 52-53 -2.6017342147962204 2315 54-55 -4.303350558976021 2315 56-57 -6.322305397219722 2315 58-59 -7.153983844421848 2315 60-61 -6.091723139829707 2315 62-63 -6.707608908830437 2315 64-65 -6.024117992989083 2315 66-67 -4.285172771001076 2315 68-69 -2.051444370367456 2315 70-71 0.22592227648236474 2315 72-73 -1.299445088723207 2315 74-75 -2.9136261651021833 2315 76-77 -5.349158810243612 2315 78-79 -4.093194495447541 2315 80-81 -4.158627412080101 2315 82-83 -6.969719334065804 2315 84-85 -5.252186537533557 2315 86-87 -5.8211734847076535 2315 88-89 -4.070230242294308 2315 90-91 -3.683121854474283 2315 92-93 -4.513350634365402 2315 94-95 -2.4968187191884397 2315 96-97 -1.6672903037871016 2315 98-99 -2.8704984680187415 2315 100-101 -2.1826890908861536 2316 1 -0.2185356895111923 2316 2 -0.3088781494830002 2316 3 -0.7965252746309552 2316 4 -0.35125194279624594 2316 5 -0.23596439110374234 2316 6 -2.1798181846822544 2316 7 -0.4663135153896718 2316 8 -0.15498705846843563 2316 9 -0.04883578882470374 2316 10-11 -0.060844166072811845 2316 12-13 -0.680270662530539 2316 14-15 -1.02953436468502 2316 16-17 -1.4661393514918402 2316 18-19 0.03388879039792414 2316 20-21 -3.3795609904301642 2316 22-23 -4.12151538887624 2316 24-25 -2.625510449035197 2316 26-27 -2.024460862245995 2316 28-29 -1.3203312825939264 2316 30-31 -0.15397497988762865 2316 32-33 -1.9088697647621515 2316 34-35 -2.724439297382908 2316 36-37 -4.969808640037172 2316 38-39 -2.1733003564108913 2316 40-41 -1.5878795535380377 2316 42-43 -3.310034788224442 2316 44-45 -0.9629725561138756 2316 46-47 -1.0717157339096417 2316 48-49 -0.8078906364594118 2316 50-51 -0.38977463451976035 2316 52-53 -2.8922984441200015 2316 54-55 -1.2879708328933432 2316 56-57 -0.5127868964504358 2316 58-59 -0.18762422074287954 2316 60-61 -1.558442942084607 2316 62-63 -1.0782422480490403 2316 64-65 -0.2487196021902811 2316 66-67 0.7378176191923806 2316 68-69 3.6350585176190258 2316 70-71 3.650827061207565 2316 72-73 1.9181638098063125 2316 74-75 0.6145050932527987 2316 76-77 -0.7100935594049602 2316 78-79 -0.2870220233815566 2316 80-81 -0.3703624740015563 2316 82-83 -0.42932838393086215 2316 84-85 -0.21135469653676253 2316 86-87 -0.5292431999446201 2316 88-89 -0.4045269103902527 2316 90-91 -0.11124958166675825 2316 92-93 0.059484636903547994 2316 94-95 0.8029436840198763 2316 96-97 1.051839516880289 2316 98-99 0.17158239560271937 2316 100-101 0.11530066874257017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 20.0 4 44.0 5 142.0 6 281.0 7 714.0 8 1520.0 9 2516.0 10 3755.0 11 5615.0 12 7493.0 13 9500.0 14 11365.0 15 14480.0 16 17303.0 17 19427.0 18 17927.0 19 15713.0 20 15564.0 21 15742.0 22 16945.0 23 19013.0 24 23022.0 25 28457.0 26 35456.0 27 44117.0 28 53726.0 29 63996.0 30 75072.0 31 87578.0 32 100963.0 33 116855.0 34 136406.0 35 157089.0 36 166015.0 37 137077.0 38 54192.0 39 2836.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.490976854862886 19.93599031936486 15.104876931271145 22.46815589450111 2 16.042735511255394 21.486796237531696 38.09265677120449 24.37781148000842 3 19.57450177206351 25.362092320516826 30.2327973162609 24.83060859115876 4 13.375527254864547 17.31865612765894 35.869163659098014 33.4366529583785 5 13.723995001140102 38.24344339440721 33.17130567811754 14.861255926335154 6 31.898293433148073 36.026409768204076 16.884267134345286 15.191029664302564 7 28.66757604175547 33.5185914429428 19.847246636868395 17.96658587843333 8 27.00458341283599 34.14236591792078 19.124279908900103 19.728770760343124 9 26.819798936085277 16.911940825663866 19.980608117525904 36.28765212072496 10-11 25.373730156474767 26.7451002680762 26.299310255233983 21.58185932021506 12-13 26.67263444068696 24.148374289043247 27.14433893708667 22.034652333183125 14-15 23.461302165584755 25.269463265712094 26.02115244347192 25.248082125231235 16-17 22.22915759570562 28.93261895603345 26.595973220662273 22.24225022759866 18-19 21.95929613168791 27.09100670977459 29.403042183990525 21.54665497454697 20-21 22.966405198597776 25.962405704708658 29.099244419755376 21.97194467693819 22-23 22.650781020584084 26.041180347213484 29.082782904289623 22.225255727912806 24-25 22.809820168369715 26.29092018745035 29.10944166805373 21.789817976126198 26-27 22.59223172872961 26.24882014432299 29.242896338472264 21.916051788475137 28-29 21.913930417110915 26.403546378385258 29.819889536038502 21.86263366846532 30-31 22.05333073500276 26.395858369331037 29.37209717756342 22.178713718102784 32-33 21.906911468860528 26.575243652977797 29.043441720535462 22.474403157626206 34-35 22.788312148804174 26.12572133863127 28.901318709873003 22.184647802691554 36-37 22.732877354832524 25.931307923656504 28.998207535125438 22.33760718638553 38-39 22.539798436543816 26.107679548038366 29.380859023772892 21.971662991644926 40-41 22.26994588157352 26.15263306568794 29.62314905700894 21.954271995729595 42-43 22.178546888107046 26.457668509293907 28.835202883723483 22.528581718875568 44-45 22.320435328407928 26.63521604719828 28.370271612931848 22.674077011461947 46-47 22.433909199121555 26.843507075140245 27.88815910895733 22.834424616780876 48-49 22.861213478701707 26.878734084106764 27.63555813233652 22.624494304855013 50-51 22.564516926486295 27.015495711740073 27.879141711362145 22.540845650411487 52-53 22.898023539305928 26.87081889198077 27.705796256991018 22.525361311722286 54-55 22.16618059516817 26.680602785079664 28.051476748968756 23.10173987078341 56-57 22.6353882735808 27.426240304751797 27.36376564489349 22.57460577677391 58-59 22.876056054951906 26.57477337684155 27.459171288907264 23.089999279299274 60-61 22.67499967857996 26.50605589190001 28.27058424689931 22.548360182620716 62-63 22.95295937382013 26.548026475153492 27.98028166726887 22.51873248375751 64-65 22.671709470617234 26.678255326966337 27.92673160872506 22.723303593691373 66-67 21.90954722653961 26.943375714491978 28.472664577335 22.674412481633414 68-69 21.69261662475249 26.618129433984656 28.994789545805823 22.694464395457032 70-71 21.361986247011057 26.71162245331613 29.057072002648876 22.869319297023942 72-73 22.230013811128494 26.7902984879681 27.691295664336995 23.288392036566414 74-75 21.9705137161974 27.202746830905504 27.751625120194177 23.075114332702913 76-77 22.4316378461248 26.92140987246373 27.667923205524126 22.979029075887343 78-79 22.186659557141063 26.900464498780135 27.801955502985965 23.110920441092833 80-81 22.036868005800038 27.53090320795225 27.510705917298694 22.92152286894902 82-83 22.29274841028514 27.243497359158503 27.29363478034938 23.17011945020698 84-85 22.006505009005792 26.969196272103428 27.295867620969215 23.72843109792156 86-87 22.376145643703666 26.800459154002322 27.63232901571144 23.191066186582567 88-89 22.279118751845914 26.76844890459708 27.30866521440681 23.6437671291502 90-91 21.967877219715646 27.145454471639336 27.532864377099276 23.35380393154574 92-93 22.099981900207556 27.083717460548375 27.448791965722037 23.367508673522032 94-95 21.52895626172103 26.763702351716155 27.755838433143133 23.951502953419684 96-97 21.45293215684918 26.625108591494566 27.70671717654654 24.21524207510971 98-99 21.809352022626374 26.696428239117115 27.106444990998106 24.387774747258405 100-101 21.63419198311594 26.432737972593173 27.666393633696668 24.266676410594222 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2123.0 1 1410.5 2 773.5 3 875.0 4 843.5 5 867.0 6 1300.5 7 2348.5 8 3411.0 9 3910.0 10 3705.0 11 3166.5 12 2922.5 13 2788.0 14 2665.0 15 2649.0 16 2627.0 17 2660.5 18 2711.0 19 2765.0 20 2930.0 21 3219.0 22 3648.0 23 4172.0 24 4813.5 25 5597.5 26 6647.0 27 7851.5 28 9057.5 29 10603.0 30 12628.5 31 15107.5 32 17890.0 33 20449.0 34 23031.5 35 25663.0 36 29265.0 37 33218.5 38 35873.0 39 37147.0 40 38385.0 41 41704.5 42 44972.0 43 47876.0 44 51597.0 45 54297.0 46 56488.0 47 60848.5 48 69870.5 49 69465.5 50 60606.0 51 55649.5 52 52096.0 53 51211.0 54 50356.5 55 46504.0 56 42326.0 57 38727.5 58 34695.5 59 30803.0 60 26665.5 61 22172.0 62 18127.5 63 14532.0 64 11328.0 65 8818.0 66 6647.5 67 5047.0 68 3452.0 69 2107.5 70 1452.0 71 1153.5 72 954.5 73 792.5 74 625.0 75 474.5 76 326.0 77 195.5 78 121.5 79 77.0 80 45.0 81 29.0 82 16.5 83 11.0 84 9.0 85 3.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0236139811006957 2 0.01968959455674054 3 0.0 4 0.0 5 6.766183696474412E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.07463777235581E-4 18-19 0.0013194058208125105 20-21 6.766183696474412E-5 22-23 0.0 24-25 0.0 26-27 2.0298551089423236E-4 28-29 0.017693570366280588 30-31 0.004465681239673112 32-33 0.00805175859880455 34-35 0.008796038805416736 36-37 0.10010568778933893 38-39 0.10348877963757613 40-41 0.11712263978597207 42-43 0.10240619024614023 44-45 0.09766986165860815 46-47 0.09915842207183251 48-49 0.1018310646319399 50-51 0.09912459115335015 52-53 0.07655936852560798 54-55 0.06498919440463673 56-57 0.03646973012399708 58-59 0.014175154844113892 60-61 0.008187082272734039 62-63 0.001623884087153859 64-65 0.003822893788508043 66-67 0.4178456741757773 68-69 0.8015897825213235 70-71 0.8908019145593388 72-73 0.2792065702350166 74-75 0.14892370315940182 76-77 0.01363386014839594 78-79 0.0073413093106747375 80-81 0.00115025122840065 82-83 0.0 84-85 0.0 86-87 1.691545924118603E-4 88-89 7.781111250945575E-4 90-91 0.00311244450037823 92-93 0.0013870676577772546 94-95 0.2227089363694553 96-97 0.22835869975601142 98-99 0.01309256545267799 100-101 0.0063940435931683194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477938.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.3623216782907 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30124136318709 29.78123801010487 2 14.635995233706437 12.400294763444029 3 5.714875771723219 7.262862173796264 4 2.918884998314767 4.946029809621886 5 1.6418675208295277 3.4776660035259046 6 1.0360804086672384 2.6334462933921863 7 0.6717988010383983 1.9921269838875044 8 0.4968769303160714 1.683908828525684 9 0.36873088707722473 1.4058266805978188 >10 1.9093347869016526 15.104782664749361 >50 0.17947464394630508 5.293653320571614 >100 0.1141243147686692 8.599955052002272 >500 0.007792246926134397 2.193774104400482 >1k 0.002435077164416999 1.8338301024433619 >5k 4.870154328833998E-4 1.3906052089367442 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8144 0.5510380002408761 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7024 0.4752567428403627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5053 0.34189526218285204 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3199 0.21645021645021645 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3109 0.21036065112338947 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2819 0.19073871840361367 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2310 0.15629884338855893 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1832 0.12395648531941124 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.11881418571009067 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1609 0.1088678956762733 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1525 0.10318430137123479 No Hit CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA 1494 0.10108678442532773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.0149275544711618E-4 0.0 24-25 0.0 0.0 0.0 4.736328587532089E-4 0.0 26-27 0.0 0.0 0.0 0.0017253768426009751 0.0 28-29 0.0 0.0 0.0 0.004161202973331763 0.0 30-31 0.0 0.0 0.0 0.010047782789264503 0.0 32-33 0.0 0.0 0.0 0.019283623534952075 0.0 34-35 0.0 0.0 0.0 0.03477818419987848 0.0 36-37 0.0 0.0 0.0 0.056497633865561336 0.0 38-39 0.0 0.0 0.0 0.08873849917926191 0.0 40-41 0.0 0.0 0.0 0.1258171858359417 0.0 42-43 0.0 0.0 0.0 0.16164412850877372 0.0 44-45 0.0 0.0 0.0 0.20362829834539742 0.0 46-47 0.0 0.0 0.0 0.2578930915911222 0.0 48-49 0.0 0.0 0.0 0.3163529187286611 0.0 50-51 0.0 0.0 0.0 0.38042867833427385 0.0 52-53 0.0 0.0 0.0 0.4425422446679089 0.0 54-55 0.0 0.0 0.0 0.5120986130676659 0.0 56-57 0.0 0.0 0.0 0.5935634647732178 0.0 58-59 0.0 0.0 0.0 0.6767875242398531 0.0 60-61 0.0 0.0 0.0 0.7637329847395493 0.0 62-63 0.0 0.0 0.0 0.8446565417493832 0.0 64-65 0.0 0.0 0.0 0.9325154370481035 0.0 66-67 0.0 0.0 0.0 1.0290012165598288 0.0 68-69 0.0 0.0 0.0 1.1457517162424946 0.0 70-71 0.0 0.0 0.0 1.2987351296197809 0.0 72-73 0.0 0.0 0.0 1.4661643451890405 0.0 74-75 0.0 0.0 0.0 1.6426264159930932 0.0 76-77 0.0 0.0 0.0 1.8404696272780048 0.0 78-79 0.0 0.0 0.0 2.0714671386756414 0.0 80-81 0.0 0.0 0.0 2.3330139694628595 0.0 82-83 0.0 0.0 0.0 2.595271249538208 0.0 84-85 0.0 0.0 0.0 2.874139510588401 0.0 86-87 0.0 0.0 0.0 3.1548684721551243 0.0 88-89 0.0 0.0 0.0 3.4501447286692675 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8590 0.0 24.610697 1 TACATGG 8870 0.0 23.616055 2 ACATGGG 8835 0.0 23.009155 3 GAGTACT 6500 0.0 22.089909 12-13 CCCTATA 200 5.507063E-7 21.32658 2 CGCAATA 80 4.5592576E-5 21.02767 62-63 AGTACTT 6820 0.0 20.740755 12-13 CATGGGA 5905 0.0 20.623537 4 TAGGTCG 140 4.9319136E-4 20.30828 5 GTACTTT 7155 0.0 20.20016 14-15 GTATAGG 355 3.6379788E-12 20.028345 1 GTATCAA 14610 0.0 19.498735 1 TAGGACA 535 0.0 19.485825 4 GGTATCA 11840 0.0 19.096285 1 CGTATAG 125 0.006105493 18.960169 1 AGTACAT 6555 0.0 18.074903 2 ATGGGAG 2470 0.0 18.033535 5 ACTTTTT 8140 0.0 17.988657 16-17 CATGGGG 3395 0.0 17.726128 4 CGCGGAA 270 0.0 17.550365 82-83 >>END_MODULE