FastQCFastQC Report
Fri 27 May 2016
SRR939374_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939374_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61140.4161966811820421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45150.30734838330666014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.22184903238015624No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23150.15758837372201956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20770.14138706359422662No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA19170.13049542653352547No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15000.10210909744407315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG77550.026.6402471
TCGCGTA1308.491701E-626.1293459
TACATGG79650.025.1406042
ACATGGG83000.024.1817673
GAGTACT52150.023.79131912-13
GTACTTT58400.021.81084414-15
GTATCAA137050.021.7292441
AGTACTT56700.021.45806712-13
CATGGGG31200.021.0890164
CATGGGA55350.020.7601874
TACCTGG8150.020.424882
GTATCGA1556.471668E-419.5054381
ACTTTTT71500.019.26492516-17
ATGGGAG20650.018.817185
CGACGCA1300.00665031318.6814795
ATGGGGA18100.018.5163385
TATCAAC158200.018.4759182
TCTATAC2352.2285367E-618.4333693
TATCGAC1609.7223127E-418.3598652
TACTTTT67950.018.18692614-15
Produced by FastQC (version 0.11.5)