FastQCFastQC Report
Fri 27 May 2016
SRR939374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939374_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469017
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79160.5388637435781887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66290.451254137971174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51530.35077878608620594No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31630.21531405014373556No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29950.2038778312299994No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27620.18801688476035336No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23000.1565672827475788No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT18410.12532189892969245No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.11701702567090781No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14990.10204102471244376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG804.5183883E-429.6603452
ATCACGC909.0074074E-426.3593643
GTAAGGT3800.023.7258534
GTACATG85450.022.9470391
TACATGG88500.021.8782752
AGGTAAG4350.021.8191072
TTCGGCG1100.002909687621.549878
GAGTACT62400.021.49932312-13
ACATGGG87300.021.250543
AGTACTT67000.020.05862612-13
GTACTTT68050.019.85361914-15
AACGCAT1352.035813E-819.56042964-65
CATGGGA55750.019.4913734
GTATCAA138550.019.4639241
TCGCGCT2600.019.17658260-61
ATTCGCG2600.019.14557680-81
TAAGGTG5450.019.1378655
GGTATCA112150.019.134971
TATTTCG1250.006098573618.9638825
ACACCGT1751.0085347E-418.9638826
Produced by FastQC (version 0.11.5)