FastQCFastQC Report
Fri 27 May 2016
SRR939373_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939373_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731400
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119870.4388591930877938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88780.32503478069854286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62430.22856410631910376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35110.12854213956212932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA175300.031.758421
GGTATCA117150.030.6596241
TATCAAC210200.026.0322672
ATCAACG217100.025.4059853
GTACATG133950.025.2128891
TCAACGC221600.024.9597574
CAACGCA221700.024.613285
TACATGG131350.024.5950872
AACGCAG225450.024.079656
GAGTACT113350.022.85258312-13
ACATGGG138850.022.2411463
ACGCAGA245200.021.815177
CGCAGAG247650.021.5250828
AGTACTT118750.021.00698312-13
CATGGGA88750.020.9716954
GTACTTT125200.020.94702314-15
GCAGAGT245300.020.5773289
ACTTTTT151850.018.62850816-17
AGAGTAC224100.017.77246510-11
CAGAGTA241000.017.30830610-11
Produced by FastQC (version 0.11.5)