FastQCFastQC Report
Fri 27 May 2016
SRR939373_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939373_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731400
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158200.5791901588928754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137110.5019770081277001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99000.3624514900783481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55360.20267994435088232No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35240.1290180859632423No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30920.11320202094164165No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29230.10701471772717287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28050.10269458885553197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122600.035.5290681
GTATCAA179250.029.588961
TATCAAC224800.023.5461672
ATCAACG227250.023.1625353
TCAACGC229750.022.9311164
CAACGCA233900.022.5242565
GTACATG146500.022.420971
TACATGG145400.022.1619452
AACGCAG239500.021.9975936
GAGTACT137650.021.66802212-13
ACATGGG148200.020.97163
AGTACTT141200.020.5360312-13
GTACTTT146000.020.34765814-15
ACGCAGA266450.019.6659687
CGCAGAG267550.019.5851128
ACCGTCG1507.859631E-418.9511648
CATGGGA94050.018.6388384
GCAGAGT276050.018.5186619
ACTTTTT169450.018.13260716-17
TAGCCCT19600.018.1293034
Produced by FastQC (version 0.11.5)