##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939373_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2731400 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.942551072709964 31.0 31.0 31.0 28.0 34.0 2 31.211489712235483 31.0 31.0 34.0 30.0 34.0 3 31.19320421761734 31.0 31.0 34.0 28.0 34.0 4 35.12997437211686 35.0 35.0 37.0 32.0 37.0 5 34.75428791096141 35.0 35.0 37.0 32.0 37.0 6 34.71570696346196 35.0 35.0 37.0 31.0 37.0 7 34.584581899392255 35.0 35.0 37.0 30.0 37.0 8 34.60325181225745 35.0 35.0 37.0 30.0 37.0 9 35.94289045910522 37.0 35.0 39.0 30.0 39.0 10-11 35.8042492860804 37.0 35.0 39.0 30.0 39.0 12-13 35.771607600497916 37.0 35.0 39.0 30.0 39.0 14-15 36.601085157794536 38.0 35.0 40.0 30.0 41.0 16-17 36.60849692465403 38.0 35.0 40.0 30.5 41.0 18-19 36.59405396499963 38.0 35.0 40.0 30.0 41.0 20-21 36.50362433184448 38.0 35.0 40.0 30.0 41.0 22-23 36.35391008274145 38.0 34.5 40.0 30.0 41.0 24-25 36.14172255985941 38.0 34.0 40.0 29.0 41.0 26-27 35.74548143809036 38.0 34.0 40.0 27.5 41.0 28-29 35.461907263674306 38.0 34.0 40.0 27.0 41.0 30-31 35.233885919308776 38.0 34.0 40.0 27.0 41.0 32-33 35.00526506553416 37.5 33.0 40.0 27.0 41.0 34-35 34.96151570623124 37.5 33.0 40.0 26.0 41.0 36-37 34.94399593614996 38.0 33.0 40.0 25.5 41.0 38-39 34.897452222303585 38.0 33.0 40.0 26.0 41.0 40-41 34.67038368602182 38.0 33.0 40.0 25.0 41.0 42-43 34.356435893680896 37.5 33.0 40.0 24.0 41.0 44-45 34.15020099582632 37.0 33.0 40.0 24.0 41.0 46-47 33.79754265211979 37.0 32.5 40.0 23.5 41.0 48-49 33.46536647872885 37.0 32.0 40.0 22.5 41.0 50-51 33.19757761587465 37.0 31.5 40.0 20.5 41.0 52-53 32.82393168338581 37.0 31.0 39.5 19.0 41.0 54-55 32.60507011056601 36.0 31.0 39.0 18.5 40.5 56-57 32.21561854726514 36.0 30.5 39.0 16.5 40.0 58-59 31.79368144541261 35.5 30.0 39.0 13.5 40.0 60-61 31.50628578750824 35.0 29.5 39.0 12.5 40.0 62-63 31.114442410485466 35.0 29.0 38.0 8.5 40.0 64-65 30.669399392253055 34.5 28.5 38.0 8.0 40.0 66-67 28.94422951599912 33.5 25.0 37.5 4.5 39.5 68-69 25.217867577066706 29.5 15.5 35.0 2.0 39.0 70-71 24.59708189939225 29.0 13.0 35.0 2.0 38.0 72-73 26.465310646554883 31.0 21.0 35.0 2.0 38.0 74-75 27.089798271948453 31.5 23.0 35.0 2.0 38.0 76-77 27.18815351101999 31.5 25.0 34.5 2.0 36.5 78-79 28.0231154353079 32.5 26.0 35.0 2.0 37.0 80-81 27.88498480632643 33.0 26.0 35.0 2.0 37.0 82-83 27.467588416196822 32.5 26.0 35.0 2.0 36.0 84-85 27.114493117082816 32.0 25.5 35.0 2.0 36.0 86-87 26.651706633960607 32.0 25.0 34.5 2.0 35.5 88-89 26.141790107637107 32.0 24.0 34.0 2.0 35.0 90-91 25.667249945083107 31.5 23.0 34.0 2.0 35.0 92-93 25.09307845793366 31.0 20.0 34.0 2.0 35.0 94-95 23.684475909789853 31.0 7.0 34.0 2.0 35.0 96-97 22.862587134802666 30.0 2.0 34.0 2.0 35.0 98-99 22.52841546459691 30.0 2.0 34.0 2.0 35.0 100-101 21.259363696272974 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38155922362927086 1101 2 0.21806420827218886 1101 3 0.2895209862249537 1101 4 0.23042850790034208 1101 5 0.3639891407054421 1101 6 0.5611404246938321 1101 7 0.5173652494120944 1101 8 0.3408265246930213 1101 9 0.6712296558285189 1101 10-11 0.7124983902555471 1101 12-13 0.6888333266622482 1101 14-15 0.7542569479165522 1101 16-17 0.5737232172154734 1101 18-19 0.7881091471754615 1101 20-21 0.831175435661649 1101 22-23 0.785221994726534 1101 24-25 0.7315445748381393 1101 26-27 0.8400006792809123 1101 28-29 0.7992778800195808 1101 30-31 0.9107609423428684 1101 32-33 0.8718407227552802 1101 34-35 0.7921199824616068 1101 36-37 0.6850944065094495 1101 38-39 0.4426550142336225 1101 40-41 0.7060258416904546 1101 42-43 0.6923755833454877 1101 44-45 0.43512766044712947 1101 46-47 0.39516539696586506 1101 48-49 0.276107125075022 1101 50-51 0.37180571657555106 1101 52-53 0.4671147900037127 1101 54-55 0.34623915981533315 1101 56-57 0.52122859507584 1101 58-59 0.5348954791183509 1101 60-61 0.33621748093057846 1101 62-63 0.25307568051007223 1101 64-65 -0.16996214315715363 1101 66-67 -3.039984175038459 1101 68-69 -8.089158198840394 1101 70-71 -11.117098918067974 1101 72-73 -11.200022056572235 1101 74-75 -9.87698098209421 1101 76-77 -4.895407686404383 1101 78-79 -1.8093780646126874 1101 80-81 -1.2131678072949477 1101 82-83 -0.8937265204120486 1101 84-85 -1.036550412583324 1101 86-87 -1.0205547065547158 1101 88-89 -0.7681872821380118 1101 90-91 -0.9285975747033568 1101 92-93 -1.5475396194085462 1101 94-95 -4.986641510264121 1101 96-97 -1.9440855940674773 1101 98-99 -1.1504259985002427 1101 100-101 -1.0165525933218689 1102 1 0.38829247451892357 1102 2 0.30885329356437197 1102 3 0.39588645475940965 1102 4 0.29291092385955864 1102 5 0.4429668363605117 1102 6 0.563495643576104 1102 7 0.4978756733026657 1102 8 0.5136952168920033 1102 9 0.6528564056630799 1102 10-11 0.6951928460977612 1102 12-13 0.6440027956206578 1102 14-15 0.5463196517668152 1102 16-17 0.6867997118360591 1102 18-19 0.7470316581856409 1102 20-21 0.8427763762438119 1102 22-23 0.7807446376818632 1102 24-25 0.5988722624271787 1102 26-27 0.7584358537501927 1102 28-29 0.7283814687608228 1102 30-31 0.6184385592092312 1102 32-33 0.32824979665941356 1102 34-35 0.5181831570505082 1102 36-37 0.7252983132332176 1102 38-39 0.6670486331009187 1102 40-41 0.6219807382571432 1102 42-43 0.794375225714326 1102 44-45 0.6151555365553989 1102 46-47 0.646024027075768 1102 48-49 0.41072570981173584 1102 50-51 0.53573556227947 1102 52-53 0.6421889252350823 1102 54-55 0.4922902953756534 1102 56-57 0.39193109619521493 1102 58-59 0.1900079773103549 1102 60-61 0.08405531777874842 1102 62-63 0.16364830943885167 1102 64-65 0.11361058402865609 1102 66-67 -2.6138222450182127 1102 68-69 -7.478598447074667 1102 70-71 -11.06937931514028 1102 72-73 -9.284532863726241 1102 74-75 -7.951483100723191 1102 76-77 -4.257274988968284 1102 78-79 -1.4564445227764544 1102 80-81 -0.7878223972305385 1102 82-83 -0.519434789808809 1102 84-85 -0.4848699631479576 1102 86-87 -0.46157384144940394 1102 88-89 -0.4349876875347043 1102 90-91 -0.4400361775901622 1102 92-93 -0.8143246861307603 1102 94-95 -4.268321111393487 1102 96-97 -1.1862677015647343 1102 98-99 -0.2787271446898103 1102 100-101 -0.13355423236298947 1103 1 0.4131918181685563 1103 2 0.32318545400350374 1103 3 0.40966781822336173 1103 4 0.1589889062237333 1103 5 0.4248863005497441 1103 6 0.3816998429753369 1103 7 0.4307682340814054 1103 8 0.4845056092090658 1103 9 0.74293990389301 1103 10-11 0.5839059926325376 1103 12-13 0.5252363500387602 1103 14-15 0.7753758777010873 1103 16-17 0.6483833690490144 1103 18-19 0.5993824355827826 1103 20-21 0.6326872657763687 1103 22-23 0.6516153433306329 1103 24-25 0.8768398367733852 1103 26-27 0.7076271357911494 1103 28-29 0.7349685086021438 1103 30-31 0.9124832099036269 1103 32-33 0.8849484789275053 1103 34-35 0.7914216316921241 1103 36-37 0.7021151359354079 1103 38-39 0.7940725791537986 1103 40-41 0.8820349089017583 1103 42-43 0.9070038432868017 1103 44-45 0.6276450427139295 1103 46-47 0.7726559061032958 1103 48-49 0.7086230036381878 1103 50-51 0.6027487555930122 1103 52-53 0.6592512813038169 1103 54-55 0.6697742697751821 1103 56-57 0.7189004687047529 1103 58-59 0.7100112409924897 1103 60-61 0.5500429718685318 1103 62-63 0.6399704676640638 1103 64-65 0.4571697164520536 1103 66-67 -3.0144681787324608 1103 68-69 -7.792673693776745 1103 70-71 -11.423551134034671 1103 72-73 -9.238622250351401 1103 74-75 -7.745372767008657 1103 76-77 -3.908551109940344 1103 78-79 -0.9255220456538602 1103 80-81 -0.3671713776791208 1103 82-83 -0.03876755105294549 1103 84-85 -0.028894696675497045 1103 86-87 0.06474693497490236 1103 88-89 0.21759729752311685 1103 90-91 0.15018474149876582 1103 92-93 0.22634930674643527 1103 94-95 0.9396384059905323 1103 96-97 1.0023652296560996 1103 98-99 0.7603699754650144 1103 100-101 0.740458931492384 1104 1 0.3957910158048996 1104 2 0.21068145649023506 1104 3 0.37020634244073136 1104 4 0.18392748375146084 1104 5 0.3480336561431727 1104 6 0.42810367952920103 1104 7 0.47911388720508086 1104 8 0.3919822037116063 1104 9 0.593699815765973 1104 10-11 0.6380955239890795 1104 12-13 0.5652895239650277 1104 14-15 0.7747752198333444 1104 16-17 0.7368829420876182 1104 18-19 0.7819534609957088 1104 20-21 0.8157984709193329 1104 22-23 0.580261220518679 1104 24-25 0.6686927698256113 1104 26-27 0.8319353661835294 1104 28-29 0.8462513364458317 1104 30-31 0.8442577616603373 1104 32-33 0.7122458769116236 1104 34-35 0.7649314946684242 1104 36-37 0.8487371519527542 1104 38-39 0.8244716503562088 1104 40-41 0.8938256003796639 1104 42-43 0.9410564456233956 1104 44-45 0.7463693286875568 1104 46-47 0.8014758768396177 1104 48-49 0.7887738446661032 1104 50-51 0.9467529336752634 1104 52-53 0.994194683846608 1104 54-55 0.8014132344342642 1104 56-57 0.8360831221494394 1104 58-59 0.847034134411178 1104 60-61 0.7899409593006865 1104 62-63 0.9527128447460669 1104 64-65 0.5737575663941854 1104 66-67 -2.533042806769007 1104 68-69 -7.26730174050293 1104 70-71 -8.68778887379765 1104 72-73 -7.937741835279965 1104 74-75 -6.950701491649966 1104 76-77 -3.331159965752015 1104 78-79 -0.43706726105428473 1104 80-81 0.2539553870429039 1104 82-83 0.3283748599945575 1104 84-85 0.3899940409614828 1104 86-87 0.5953109974604267 1104 88-89 0.4936800979989222 1104 90-91 0.518152821667119 1104 92-93 0.5933785503695788 1104 94-95 1.5379319247195617 1104 96-97 1.5811232408457805 1104 98-99 1.1081195218535278 1104 100-101 1.0097926161555009 1105 1 0.46145381329517576 1105 2 0.33894491326575604 1105 3 0.4557116128836043 1105 4 0.2539103946296706 1105 5 0.4098215704921202 1105 6 0.39964245793195374 1105 7 0.48663247823967026 1105 8 0.5381598745483487 1105 9 0.6391932991764548 1105 10-11 0.7668295282286692 1105 12-13 0.7031125649156209 1105 14-15 0.8829699050853392 1105 16-17 0.832814652006256 1105 18-19 0.8784374805397732 1105 20-21 0.9531544403353109 1105 22-23 0.9809070689619261 1105 24-25 1.0065169521261765 1105 26-27 1.061546497873529 1105 28-29 1.1275615750813586 1105 30-31 0.9669825064389173 1105 32-33 1.0061199403561574 1105 34-35 1.1129886057456346 1105 36-37 1.0422934531902897 1105 38-39 0.7832588518802268 1105 40-41 1.0337041088635246 1105 42-43 1.0991925588702145 1105 44-45 0.848839884452353 1105 46-47 1.0363719776768505 1105 48-49 0.8997172637978323 1105 50-51 0.8743506202626392 1105 52-53 1.0808005788154347 1105 54-55 1.1138069980980418 1105 56-57 1.1113769419468795 1105 58-59 1.2724501824142784 1105 60-61 1.3186812409651054 1105 62-63 1.3051232721193742 1105 64-65 1.3464373834876646 1105 66-67 1.926706345259845 1105 68-69 -4.969669678529698 1105 70-71 -5.281893023019823 1105 72-73 -1.5104473327477237 1105 74-75 1.1925851915974022 1105 76-77 1.326741185040028 1105 78-79 1.3696175219491487 1105 80-81 1.3974496403458154 1105 82-83 1.3940578386274467 1105 84-85 1.2743612298476386 1105 86-87 1.5131550184986189 1105 88-89 1.4551273193824237 1105 90-91 1.2942554435555103 1105 92-93 1.430001824985645 1105 94-95 2.2297740220642623 1105 96-97 2.1246751323220714 1105 98-99 1.7495363106965236 1105 100-101 1.7812901503207783 1106 1 0.1442851589759968 1106 2 0.10901156390212563 1106 3 0.127120228230428 1106 4 0.0735523985831108 1106 5 0.25625613097577826 1106 6 0.4337995818888132 1106 7 0.3906867897124684 1106 8 0.2699326482370097 1106 9 0.45464577507636506 1106 10-11 0.41488793679509683 1106 12-13 0.3954736608465268 1106 14-15 0.32024627466066846 1106 16-17 0.3814830445319757 1106 18-19 0.289932034923595 1106 20-21 0.4304245227039587 1106 22-23 0.6391553844797713 1106 24-25 0.5642159698021842 1106 26-27 0.500955052846507 1106 28-29 0.7617578899871376 1106 30-31 0.6097405603610895 1106 32-33 0.6768282191708721 1106 34-35 0.7944782469480742 1106 36-37 0.7024642591081687 1106 38-39 0.6934784773334215 1106 40-41 0.7334984734861223 1106 42-43 0.7623528586991881 1106 44-45 0.5697168977377274 1106 46-47 0.4959922366389833 1106 48-49 0.2871007448909282 1106 50-51 0.42212220104752163 1106 52-53 0.6933730664570064 1106 54-55 0.6883040996333065 1106 56-57 0.5152412840125677 1106 58-59 0.6112654082800937 1106 60-61 0.507635690985456 1106 62-63 0.5898229278843985 1106 64-65 0.6340808364132435 1106 66-67 1.251401119071339 1106 68-69 -5.473595695594675 1106 70-71 -6.244246967773595 1106 72-73 -2.454524034587358 1106 74-75 0.22287198815109832 1106 76-77 0.5980460718859248 1106 78-79 0.3938691726011321 1106 80-81 0.43948430117495363 1106 82-83 0.4715754016894813 1106 84-85 0.491107007061931 1106 86-87 0.6369906621722237 1106 88-89 0.6225409041207506 1106 90-91 0.4222196689331348 1106 92-93 0.48678211938097604 1106 94-95 1.4560220169762026 1106 96-97 1.4265192928706831 1106 98-99 1.3245410208968913 1106 100-101 1.226435885481564 1107 1 -0.05111199598916727 1107 2 -0.06929908902581516 1107 3 -0.1197221603922003 1107 4 -0.04503361298861108 1107 5 0.09456512218780944 1107 6 0.016972190846544777 1107 7 -0.03578762931631729 1107 8 0.026372742784111836 1107 9 0.09973610143715206 1107 10-11 0.02132564897314637 1107 12-13 0.15198862984926365 1107 14-15 0.11160660522328669 1107 16-17 -0.04767318734027981 1107 18-19 0.07313131993196009 1107 20-21 0.23672155085793634 1107 22-23 0.1417485472642923 1107 24-25 0.3901839112181591 1107 26-27 0.3803556963346537 1107 28-29 0.3202856745146647 1107 30-31 0.1177819472434507 1107 32-33 0.30498633025150923 1107 34-35 0.26047200870663545 1107 36-37 0.1811434607455098 1107 38-39 -0.05307635232286856 1107 40-41 -0.013661780430119563 1107 42-43 0.1644121007082333 1107 44-45 0.049857339743347495 1107 46-47 0.050340287034572384 1107 48-49 0.02032786742238102 1107 50-51 -0.035139124740311445 1107 52-53 0.12442222828988037 1107 54-55 0.06351620340779363 1107 56-57 0.33700307455143985 1107 58-59 0.18943569051141296 1107 60-61 0.056028791169314474 1107 62-63 0.1885571606259724 1107 64-65 0.0684635321810454 1107 66-67 0.9640930848045528 1107 68-69 -5.616554098782924 1107 70-71 -6.301830070045941 1107 72-73 -2.5793935580152265 1107 74-75 0.07223679238141045 1107 76-77 -0.022895837961556254 1107 78-79 0.01399605293046946 1107 80-81 0.02348625547388039 1107 82-83 0.19645980276892772 1107 84-85 0.2978929340784582 1107 86-87 0.3488768361448038 1107 88-89 0.34061362050657706 1107 90-91 0.3139413200650658 1107 92-93 0.31289554197229563 1107 94-95 1.0919318491158876 1107 96-97 1.5715753152781353 1107 98-99 1.1551914249326352 1107 100-101 0.860321244154516 1108 1 0.04770058220593398 1108 2 0.05903254485698639 1108 3 0.1392049129534101 1108 4 0.04848477020572517 1108 5 0.07502416426563485 1108 6 0.26734491871648913 1108 7 0.18428199312846516 1108 8 0.16144260693436507 1108 9 0.28729177398291483 1108 10-11 0.28048299044731095 1108 12-13 0.26810275630032976 1108 14-15 0.30617382114921554 1108 16-17 0.13871176305150357 1108 18-19 0.18574894288652644 1108 20-21 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2311 5 -0.018700069680001263 2311 6 0.0015699802478295055 2311 7 -0.061868535309891115 2311 8 -0.13900147542853603 2311 9 0.08274392330960012 2311 10-11 -1.3868121375489153 2311 12-13 -0.23035057388732127 2311 14-15 0.03654222273532781 2311 16-17 -0.09950868808407165 2311 18-19 0.030169405572664232 2311 20-21 0.0970571395778137 2311 22-23 0.17446124940931185 2311 24-25 0.01591451249461784 2311 26-27 0.24543639438478237 2311 28-29 0.06544699063667991 2311 30-31 0.2670109569528094 2311 32-33 0.18824768316351026 2311 34-35 0.36785719993461896 2311 36-37 0.41985133220447324 2311 38-39 0.18999273288594765 2311 40-41 0.4360494981021148 2311 42-43 0.3511005214577878 2311 44-45 0.44178593516539166 2311 46-47 0.5047278936055832 2311 48-49 0.5375119933401322 2311 50-51 0.46635246954603815 2311 52-53 0.5745805852168004 2311 54-55 0.5269333539428445 2311 56-57 0.6473490604432754 2311 58-59 0.7731721142041756 2311 60-61 0.8643265341832134 2311 62-63 0.8902063120567831 2311 64-65 0.9111943094096411 2311 66-67 2.1185054846318003 2311 68-69 4.532492560525746 2311 70-71 4.991955620246298 2311 72-73 2.9562175557618247 2311 74-75 2.0393329422286506 2311 76-77 1.238389892319809 2311 78-79 1.0663706070517485 2311 80-81 0.9373016976736004 2311 82-83 0.6906597803750181 2311 84-85 0.7940827704429658 2311 86-87 0.8949786964388018 2311 88-89 0.6986210424877974 2311 90-91 0.8276527905450912 2311 92-93 1.0578353183531206 2311 94-95 1.7982277366344839 2311 96-97 1.8863185051304932 2311 98-99 1.4574740040711092 2311 100-101 1.341480768763823 2312 1 -0.2684281109194018 2312 2 -0.252318982355316 2312 3 -0.16915021077218384 2312 4 -0.11182779099295459 2312 5 0.030059705723374464 2312 6 0.07103903483798746 2312 7 0.07861209132990865 2312 8 0.0012028415923879265 2312 9 -1.6391048161861974 2312 10-11 -1.221114455761267 2312 12-13 -1.7705206690747133 2312 14-15 -1.9283892702238532 2312 16-17 -0.12309513623799262 2312 18-19 -2.0705080599836876 2312 20-21 -1.670501829711263 2312 22-23 -0.6881647059502072 2312 24-25 -1.3474681256285095 2312 26-27 -1.5166806696353987 2312 28-29 -0.06434565040164131 2312 30-31 0.2823239599820724 2312 32-33 0.2360524159163404 2312 34-35 0.5940711244184413 2312 36-37 0.595963224925363 2312 38-39 0.3226663453173515 2312 40-41 0.6306563906328861 2312 42-43 0.526225050907577 2312 44-45 0.7926966161798887 2312 46-47 0.6615595855583791 2312 48-49 0.7610908366421612 2312 50-51 0.6368500803550745 2312 52-53 0.8948456143053605 2312 54-55 0.916029897537662 2312 56-57 0.9840804950985884 2312 58-59 1.1550693980045672 2312 60-61 1.2001713698550418 2312 62-63 0.8450841908100948 2312 64-65 1.2053626554810997 2312 66-67 2.351506739432363 2312 68-69 4.483156717591285 2312 70-71 5.29199744693172 2312 72-73 3.475815259096553 2312 74-75 2.563257172554266 2312 76-77 1.4471456118021706 2312 78-79 1.152650186756933 2312 80-81 1.0581618064229836 2312 82-83 1.164741177639808 2312 84-85 1.242974363279199 2312 86-87 1.6655510882914761 2312 88-89 1.6335989250435325 2312 90-91 1.6887032740869259 2312 92-93 1.7180201162541806 2312 94-95 2.4295255046104494 2312 96-97 2.697910834369882 2312 98-99 2.2858984055058933 2312 100-101 2.131895486687192 2313 1 -0.1879543566127566 2313 2 -0.25458402869546504 2313 3 -0.10336815763974272 2313 4 -0.08583735885355992 2313 5 -0.04354422567170246 2313 6 -0.048600835496756645 2313 7 -3.506748201501029 2313 8 -1.1494304055295999 2313 9 -0.8921215745067173 2313 10-11 -0.21600101837714192 2313 12-13 -1.3533361247417233 2313 14-15 -0.3493489647952046 2313 16-17 0.03387901415608496 2313 18-19 0.15648793271087413 2313 20-21 0.021336995005206916 2313 22-23 -1.1736649708480584 2313 24-25 -2.431902535322436 2313 26-27 -2.1355585890692197 2313 28-29 -0.5835177280001744 2313 30-31 -0.1362140929935478 2313 32-33 -2.6391424794725538 2313 34-35 -2.674277404495065 2313 36-37 -1.2479934356402964 2313 38-39 -0.7484216588071249 2313 40-41 -0.7069704268850145 2313 42-43 -0.5680311665263602 2313 44-45 -0.18775098781414812 2313 46-47 -0.16464399134007124 2313 48-49 -0.38498943080063697 2313 50-51 -0.14280528305432938 2313 52-53 0.06480611068823094 2313 54-55 -0.013594771011511853 2313 56-57 0.17477825508558453 2313 58-59 -0.1981711710735432 2313 60-61 0.24881281293178859 2313 62-63 0.03774891861578311 2313 64-65 -0.07644934216844135 2313 66-67 1.3002214647084358 2313 68-69 4.445496872710386 2313 70-71 4.284283761754768 2313 72-73 2.550800951984563 2313 74-75 1.7594728894177791 2313 76-77 0.9648592728875478 2313 78-79 0.7664215677584458 2313 80-81 0.714597729067993 2313 82-83 0.21426043020517582 2313 84-85 0.4071629556542966 2313 86-87 0.4434612359049517 2313 88-89 0.3390743493345454 2313 90-91 0.638940587078789 2313 92-93 0.8927158122500067 2313 94-95 1.5855122288370076 2313 96-97 1.4368127752968967 2313 98-99 1.1400879637342491 2313 100-101 1.1924183483899284 2314 1 -0.33529577097670327 2314 2 -0.23601204808333875 2314 3 -0.17948431273648424 2314 4 -0.24590393247711262 2314 5 -1.5401757421370519 2314 6 -2.6648962996557977 2314 7 -0.8399231430071694 2314 8 -0.22964542299246204 2314 9 -0.0716812690119184 2314 10-11 0.11977122205406943 2314 12-13 -0.1018927039598978 2314 14-15 -2.691591931317298 2314 16-17 -3.016772551548243 2314 18-19 -0.5011015294979586 2314 20-21 -0.09690655483883148 2314 22-23 -0.035420011755284975 2314 24-25 -2.210844033438562 2314 26-27 -1.047085418645331 2314 28-29 -0.04409700504219671 2314 30-31 -0.008284968393446945 2314 32-33 -0.03518641503153219 2314 34-35 -0.16916586758668473 2314 36-37 0.21013303506796177 2314 38-39 -1.3541796439738931 2314 40-41 -4.183344327330964 2314 42-43 -5.398362748035677 2314 44-45 -2.367140769735137 2314 46-47 -3.218275382266338 2314 48-49 -1.0698717150501196 2314 50-51 -0.9876760970894694 2314 52-53 -2.524174233190763 2314 54-55 -1.7288802587152574 2314 56-57 -4.206187388010136 2314 58-59 -5.756469917979384 2314 60-61 -3.0149797073021887 2314 62-63 -1.9882241821260997 2314 64-65 -1.025286589325912 2314 66-67 -0.7757433968339917 2314 68-69 1.1286987616388409 2314 70-71 1.1598236857489859 2314 72-73 -0.9435833479142666 2314 74-75 -0.5115257528424237 2314 76-77 -0.38251485299359445 2314 78-79 0.1427371360101226 2314 80-81 0.37626208303559494 2314 82-83 0.00961633128929762 2314 84-85 -0.1339234457867029 2314 86-87 -0.281895251622867 2314 88-89 0.00425758846153812 2314 90-91 0.388661803340721 2314 92-93 0.4801827746210563 2314 94-95 0.6202798666886267 2314 96-97 0.5792974101375812 2314 98-99 0.01562967158762163 2314 100-101 0.13202323250709114 2315 1 -0.4095921735913848 2315 2 -0.368118862678962 2315 3 -0.34691762638334467 2315 4 -0.18200856632761742 2315 5 -1.4262574246057795 2315 6 -0.45825265325901654 2315 7 -0.32415887654759956 2315 8 -0.17877398362209362 2315 9 -0.06294722764470606 2315 10-11 -1.9185163982005449 2315 12-13 -1.5171830396671524 2315 14-15 -0.338375841656422 2315 16-17 -1.4698383584137389 2315 18-19 -2.930337631250154 2315 20-21 -2.1386475066531574 2315 22-23 -1.6894485250267763 2315 24-25 -1.123819437765654 2315 26-27 -0.3907213075623943 2315 28-29 -0.7534753151277371 2315 30-31 -3.1923778998044696 2315 32-33 -2.496126714368785 2315 34-35 -1.8341670915633514 2315 36-37 -5.002030294940312 2315 38-39 -2.3561707527512183 2315 40-41 -1.703356709725142 2315 42-43 -1.4400504328510593 2315 44-45 -1.5277918554650256 2315 46-47 -1.2456962436606602 2315 48-49 -4.566073894456281 2315 50-51 -3.9689183999353546 2315 52-53 -2.636148159722474 2315 54-55 -4.177606842702616 2315 56-57 -6.63853873597084 2315 58-59 -7.377483370661839 2315 60-61 -6.198204794137588 2315 62-63 -7.017061858369281 2315 64-65 -6.417126492595376 2315 66-67 -4.3738600445758316 2315 68-69 -2.318856967139574 2315 70-71 0.20236626223588772 2315 72-73 -1.4836899051982684 2315 74-75 -3.19104106972431 2315 76-77 -5.406742168601728 2315 78-79 -4.655684530792865 2315 80-81 -4.256451917132647 2315 82-83 -7.2287348600722545 2315 84-85 -5.22216939844078 2315 86-87 -5.798032678151518 2315 88-89 -4.290110401506805 2315 90-91 -3.829798530064121 2315 92-93 -4.895490303393558 2315 94-95 -2.8388457162284446 2315 96-97 -1.7030009473468546 2315 98-99 -2.886231413318516 2315 100-101 -2.4103314541010334 2316 1 -0.5980751331269758 2316 2 -0.5301489378669331 2316 3 -1.0240738359956936 2316 4 -0.44305113000176277 2316 5 -0.30475352240011944 2316 6 -2.563122734444889 2316 7 -0.5116175517255712 2316 8 -0.5300949498553464 2316 9 -0.24970757580856429 2316 10-11 -0.3009248766616537 2316 12-13 -0.9031561633595757 2316 14-15 -1.1896448617850908 2316 16-17 -1.62978273502479 2316 18-19 -0.4424123336757333 2316 20-21 -4.290576831389494 2316 22-23 -4.37345287350481 2316 24-25 -2.9936059053591393 2316 26-27 -2.2844950938800395 2316 28-29 -1.410353443497094 2316 30-31 -0.6952702803284296 2316 32-33 -2.281342603093435 2316 34-35 -2.6726969989529152 2316 36-37 -4.908971079951272 2316 38-39 -2.685861967968748 2316 40-41 -2.2203471916279014 2316 42-43 -3.6006156635917286 2316 44-45 -1.3141800124672187 2316 46-47 -1.3110060637950411 2316 48-49 -0.833652027823895 2316 50-51 -0.8007992059389721 2316 52-53 -3.1973397783702318 2316 54-55 -1.6187592250830676 2316 56-57 -0.6314733517124722 2316 58-59 -0.21111088674549805 2316 60-61 -1.5861042079141967 2316 62-63 -1.3532070646899257 2316 64-65 -0.10852486030953301 2316 66-67 0.8370443531706684 2316 68-69 3.4256617370282534 2316 70-71 3.386328839619516 2316 72-73 2.018424176301778 2316 74-75 0.7859209221499626 2316 76-77 -0.44847756121431104 2316 78-79 0.11800539201986382 2316 80-81 -0.4294531524145846 2316 82-83 -0.2655130591311554 2316 84-85 -0.08707650238297404 2316 86-87 -0.5582671318070247 2316 88-89 0.08085547472223098 2316 90-91 0.1474737103660111 2316 92-93 0.21599906818353531 2316 94-95 0.5617469334870115 2316 96-97 0.31323140704339636 2316 98-99 -0.5748553949249562 2316 100-101 -0.5087973481809627 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 35.0 4 88.0 5 204.0 6 552.0 7 1325.0 8 2927.0 9 4709.0 10 7522.0 11 10981.0 12 14395.0 13 18261.0 14 22429.0 15 28426.0 16 33816.0 17 37399.0 18 34567.0 19 29548.0 20 28317.0 21 29114.0 22 30868.0 23 34812.0 24 41966.0 25 52328.0 26 65349.0 27 81650.0 28 99408.0 29 118151.0 30 138826.0 31 161221.0 32 186235.0 33 215185.0 34 249810.0 35 285434.0 36 300791.0 37 254087.0 38 104733.0 39 5926.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.79960040852662 20.9092683469869 16.04668101416843 22.24445023031805 2 15.652057371835598 21.671603402118066 39.35043815434326 23.325901071703075 3 19.031961631397817 25.79878450611408 30.585816797246835 24.583437065241267 4 13.073552024602767 18.24855385516585 37.063703595225896 31.61419052500549 5 13.082028384020477 38.51997293691578 34.09377475840193 14.304223920661816 6 30.554587391081494 37.52628688584609 17.44295965438969 14.476166068682728 7 27.37856044519294 33.868748627077686 20.64256425276415 18.11012667496522 8 25.971589660979717 35.577615874643044 19.509299260452515 18.941495203924728 9 26.026982499816942 17.050120817163357 20.608369334407264 36.31452734861244 10-11 24.400124478289523 27.608241194991578 27.033554221278465 20.958080105440434 12-13 25.602731200117155 25.115032584022845 27.825327670791534 21.456908545068462 14-15 22.710771033169802 26.225763344804864 26.80956652266237 24.253899099362965 16-17 21.073224559477232 29.443665128924497 27.499816485076895 21.983293826521376 18-19 20.906787036098017 27.65779724379972 30.547465391896882 20.887950328205378 20-21 22.118233959313145 26.65005246397632 30.596639523057423 20.63507405365311 22-23 21.528007615142418 26.753990627517027 30.36677161894999 21.35123013839057 24-25 21.304587391081498 26.94881745624954 30.704766786263455 21.041828366405507 26-27 21.33640088182256 27.039269587610843 30.572033235111007 21.052296295455587 28-29 20.98331079189563 27.02733333174655 30.833739030388006 21.155616845969814 30-31 21.302468304099047 27.01615147250288 30.666800182186442 21.014580041211627 32-33 21.18069591146886 27.00935356215985 30.36531837114888 21.44463215522241 34-35 21.519379214842836 27.013934199936873 30.36973449007404 21.096952095146243 36-37 21.559920757157265 26.873226294535847 30.49258871253103 21.07426423577586 38-39 21.38403124043665 26.99943009292601 30.471758447360557 21.144780219276786 40-41 21.452704838848906 26.971302959875388 30.470996982008064 21.104995219267643 42-43 21.394014260208397 27.119006531607187 30.153675927977318 21.3333032802071 44-45 21.456344071992806 27.34153723354839 29.927402134709634 21.274716559749166 46-47 21.534240169346422 27.411870814715783 29.335782201034373 21.71810681490342 48-49 21.646131120738897 27.519885478117317 29.226132524408925 21.607850876734865 50-51 21.882555371700924 27.67284015091961 28.99065639768307 21.453948079696403 52-53 21.853632244735618 27.775427691740607 28.880910606330133 21.490029457193636 54-55 21.651030623319883 27.7083755031759 29.002898842752668 21.637695030751548 56-57 21.698142577010497 27.969792795188297 28.606324775431062 21.725739852370143 58-59 21.673487203072316 27.72559034012848 28.865003178360197 21.735919278439 60-61 21.801783758341514 27.646185562933624 28.943956990908752 21.60807368781611 62-63 21.798509525526804 27.546794711030646 29.005947619213245 21.64874814422931 64-65 21.772195926990076 27.540770831178506 28.94156478344801 21.74546845838341 66-67 21.248998007045206 27.54412446039462 29.44349862846469 21.763378904095486 68-69 21.424981343555633 27.28806085387911 29.691368974171507 21.59558882839375 70-71 21.105313627637738 27.21553273925551 29.911220901980855 21.767932731125896 72-73 21.622800519685654 27.56096365449826 28.708391689655798 22.107844136160285 74-75 21.356916036580447 27.78416216255858 28.730996575166657 22.127925225694316 76-77 21.56157480072772 27.850554348325584 28.49784483666145 22.090026014285247 78-79 21.479249892307447 27.75082845001994 28.602373098995315 22.167548558677293 80-81 21.3607636646413 27.988387452972074 28.420041181010458 22.230807701376165 82-83 21.53137585121183 28.11206707183129 28.170718312953063 22.185838764003808 84-85 21.526909277293697 27.886303727026434 28.11957238046423 22.467214615215642 86-87 21.467421250005035 27.8208001253571 28.293818145073747 22.41796047956412 88-89 21.672154428868478 27.64774076698578 28.21935647559906 22.460748328546682 90-91 21.460406144338126 27.86657977259871 28.322718710355343 22.35029537270782 92-93 21.35377327923068 27.945346460414644 28.186234577687014 22.514645682667663 94-95 21.00863941545466 27.82947874162906 28.478127529125715 22.683754313790573 96-97 20.906644609829947 27.66833352843457 28.433191527150935 22.991830334584552 98-99 21.203752152584805 27.61641350719274 28.0567253468394 23.123108993383056 100-101 21.217436355581267 27.590665607299382 28.149506259176405 23.042391777942946 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4690.0 1 3067.5 2 1396.5 3 1426.0 4 1350.0 5 1281.5 6 1882.0 7 3500.5 8 5146.5 9 5963.5 10 5692.5 11 4777.0 12 4344.0 13 4191.0 14 4219.5 15 4352.5 16 4529.0 17 4922.0 18 5394.0 19 5961.0 20 6663.0 21 7537.0 22 8739.5 23 10465.0 24 12499.5 25 15015.5 26 18867.5 27 23320.0 28 26927.0 29 30944.5 30 36033.0 31 41547.0 32 48114.5 33 55237.5 34 61521.0 35 67242.5 36 74325.0 37 80715.0 38 83903.5 39 85202.0 40 86055.0 41 87093.5 42 88101.0 43 89824.0 44 92765.5 45 95867.5 46 98637.0 47 101214.0 48 106309.0 49 104692.0 50 95226.5 51 88885.0 52 85648.5 53 83425.0 54 79813.0 55 74167.5 56 67445.0 57 60625.5 58 54214.0 59 47421.0 60 40222.0 61 32984.5 62 25837.5 63 19469.0 64 14124.5 65 10349.5 66 7722.5 67 5836.0 68 4459.5 69 3422.5 70 2553.5 71 1829.0 72 1299.5 73 1020.5 74 791.5 75 531.5 76 342.5 77 216.0 78 141.0 79 93.0 80 59.5 81 47.5 82 31.5 83 12.5 84 4.5 85 2.5 86 2.0 87 2.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02236948085231017 2 0.018781577213150765 3 0.0 4 0.0 5 1.4644504649630226E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.308632935490957E-4 18-19 0.0011349491103463424 20-21 7.322252324815113E-5 22-23 0.0 24-25 0.0 26-27 4.2102950867686903E-4 28-29 0.018433770227722048 30-31 0.004832686534377974 32-33 0.00907959288277074 34-35 0.010177930731493007 36-37 0.10344511971882552 38-39 0.105623489785458 40-41 0.11940762978692246 42-43 0.10611774181738304 44-45 0.10025993995753094 46-47 0.10221864245441897 48-49 0.1039210661199385 50-51 0.1023284762392912 52-53 0.08001391227941715 54-55 0.06818847477484073 56-57 0.038734714798271945 58-59 0.015816065021600642 60-61 0.009390788606575383 62-63 0.002013619389324156 64-65 0.004667935857069635 66-67 0.4327451123965732 68-69 0.825602255253716 70-71 0.9242512996997876 72-73 0.2866844841473237 74-75 0.15596397451856192 76-77 0.014662810280442263 78-79 0.00898806472871055 80-81 0.001610895511459325 82-83 0.0 84-85 0.0 86-87 1.0983378487222669E-4 88-89 7.139196016694736E-4 90-91 0.003844182470527934 92-93 0.001793951819579703 94-95 0.22968074979863806 96-97 0.23134656220253352 98-99 0.013948890678772792 100-101 0.007688364941055868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2731400.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.83259378784204 #Duplication Level Percentage of deduplicated Percentage of total 1 70.38651767874423 35.07542743625624 2 14.829709541761513 14.780057831725735 3 5.982870393575839 8.944258500251143 4 2.9825066355075536 5.945041665471655 5 1.682620107038418 4.192466214665039 6 1.063002381853011 3.1783299534233795 7 0.6645501971493376 2.3181382018321304 8 0.46040680382979143 1.835461218592694 9 0.3333135824216846 1.4948892321111193 >10 1.4892818853691077 12.6464138346564 >50 0.08151218184879423 2.788536270126914 >100 0.04074796443004186 3.683234246064468 >500 0.0017023717206963167 0.5350049327241548 >1k 9.622101029582065E-4 0.9241698444561606 >5k 1.4803232353203177E-4 0.5693440979815332 >10k+ 1.4803232353203177E-4 1.0892265196613535 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15820 0.5791901588928754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13711 0.5019770081277001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9900 0.3624514900783481 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5536 0.20267994435088232 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3524 0.1290180859632423 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3092 0.11320202094164165 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2923 0.10701471772717287 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2805 0.10269458885553197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.6611261624075566E-5 0.0 0.0 0.0 0.0 3 3.6611261624075566E-5 0.0 0.0 0.0 0.0 4 3.6611261624075566E-5 0.0 0.0 0.0 0.0 5 3.6611261624075566E-5 0.0 0.0 0.0 0.0 6 7.322252324815113E-5 0.0 0.0 0.0 0.0 7 7.322252324815113E-5 0.0 0.0 0.0 0.0 8 7.322252324815113E-5 0.0 0.0 0.0 0.0 9 7.322252324815113E-5 0.0 0.0 0.0 0.0 10-11 7.322252324815113E-5 0.0 0.0 0.0 0.0 12-13 7.322252324815113E-5 0.0 0.0 0.0 0.0 14-15 7.322252324815113E-5 0.0 0.0 1.8305630812037783E-5 0.0 16-17 9.15281540601889E-5 0.0 0.0 9.15281540601889E-5 0.0 18-19 1.0983378487222669E-4 0.0 0.0 1.2813941568426448E-4 0.0 20-21 1.0983378487222669E-4 0.0 0.0 2.928900929926045E-4 0.0 22-23 1.0983378487222669E-4 0.0 0.0 4.3933513948890676E-4 0.0 24-25 1.4644504649630226E-4 0.0 0.0 7.688364941055869E-4 0.0 26-27 1.4644504649630226E-4 0.0 0.0 0.0015376729882111738 0.0 28-29 1.4644504649630226E-4 0.0 0.0 0.00355129237753533 0.0 30-31 1.4644504649630226E-4 0.0 0.0 0.007615142417807717 0.0 32-33 1.4644504649630226E-4 0.0 0.0 0.015614703082668227 0.0 34-35 1.8305630812037784E-4 0.0 0.0 0.028520172805154866 0.0 36-37 1.8305630812037784E-4 0.0 0.0 0.05035879036391594 0.0 38-39 1.8305630812037784E-4 0.0 0.0 0.0821739767152376 0.0 40-41 1.8305630812037784E-4 0.0 0.0 0.11428205315955187 0.0 42-43 2.1966756974445338E-4 0.0 0.0 0.1493190305337922 0.0 44-45 2.928900929926045E-4 0.0 0.0 0.19347221205242732 0.0 46-47 3.111957238046423E-4 0.0 0.0 0.24798638061067585 0.0 48-49 3.295013546166801E-4 0.0 0.0 0.3084315735520246 0.0 50-51 3.295013546166801E-4 0.0 0.0 0.3703961338507725 0.0 52-53 3.295013546166801E-4 0.0 0.0 0.435545873910815 0.0 54-55 3.295013546166801E-4 0.0 0.0 0.5046862414878817 0.0 56-57 3.295013546166801E-4 0.0 0.0 0.5814417514827561 0.0 58-59 3.295013546166801E-4 0.0 0.0 0.6621695833638427 0.0 60-61 3.295013546166801E-4 0.0 0.0 0.7435381123233507 0.0 62-63 3.295013546166801E-4 0.0 0.0 0.8237899978033243 0.0 64-65 3.295013546166801E-4 0.0 0.0 0.9123160284103391 0.0 66-67 3.295013546166801E-4 0.0 0.0 1.0041004613018965 0.0 68-69 3.295013546166801E-4 0.0 0.0 1.1146664714066046 0.0 70-71 3.295013546166801E-4 0.0 0.0 1.2651753679431792 0.0 72-73 3.478069854287179E-4 0.0 0.0 1.4280405652778794 0.0 74-75 3.6611261624075567E-4 0.0 0.0 1.6053672109540895 0.0 76-77 3.6611261624075567E-4 0.0 0.0 1.8002489565790438 0.0 78-79 3.8441824705279346E-4 0.0 0.0 2.025261770520612 0.0 80-81 4.0272387786483124E-4 0.0 0.0 2.2808815991799074 0.0 82-83 4.0272387786483124E-4 0.0 0.0 2.548070586512411 0.0 84-85 4.2102950867686903E-4 0.0 0.0 2.8157172146152156 0.0 86-87 4.3933513948890676E-4 0.0 0.0 3.094475360620927 0.0 88-89 4.3933513948890676E-4 1.8305630812037783E-5 0.0 3.3928022259647066 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12260 0.0 35.529068 1 GTATCAA 17925 0.0 29.58896 1 TATCAAC 22480 0.0 23.546167 2 ATCAACG 22725 0.0 23.162535 3 TCAACGC 22975 0.0 22.931116 4 CAACGCA 23390 0.0 22.524256 5 GTACATG 14650 0.0 22.42097 1 TACATGG 14540 0.0 22.161945 2 AACGCAG 23950 0.0 21.997593 6 GAGTACT 13765 0.0 21.668022 12-13 ACATGGG 14820 0.0 20.9716 3 AGTACTT 14120 0.0 20.53603 12-13 GTACTTT 14600 0.0 20.347658 14-15 ACGCAGA 26645 0.0 19.665968 7 CGCAGAG 26755 0.0 19.585112 8 ACCGTCG 150 7.859631E-4 18.951164 8 CATGGGA 9405 0.0 18.638838 4 GCAGAGT 27605 0.0 18.518661 9 ACTTTTT 16945 0.0 18.132607 16-17 TAGCCCT 1960 0.0 18.129303 4 >>END_MODULE