FastQCFastQC Report
Fri 27 May 2016
SRR939372_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939372_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703220
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117370.43418589681934877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84030.31085150302232156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63860.2362367842794889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36730.13587499352623908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164750.033.5876661
GGTATCA110150.029.8778321
TATCAAC203500.026.7614752
ATCAACG210100.026.0064323
TCAACGC214750.025.4173974
CAACGCA215750.025.1664245
GTACATG135050.024.8148461
AACGCAG219150.024.6194086
TACATGG134500.024.1853032
ACGCAGA239900.022.3086937
AGTACTT116450.022.12941412-13
ACATGGG139450.022.0744513
CGCAGAG242550.022.0458268
GAGTACT110700.021.67342612-13
GCAGAGT238500.020.9990189
GTACTTT122500.020.00645314-15
CATGGGA90150.019.7243124
GTATAGG8700.019.6060181
AGAGTAC219800.018.4554210-11
TACTTTT141500.018.2757814-15
Produced by FastQC (version 0.11.5)