##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939372_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2703220 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.332490141386938 31.0 27.0 31.0 10.0 33.0 2 27.401993178505634 31.0 26.0 31.0 16.0 34.0 3 27.692068718047366 31.0 27.0 31.0 16.0 34.0 4 31.544925681224612 35.0 32.0 37.0 19.0 37.0 5 31.969625853611618 35.0 32.0 37.0 19.0 37.0 6 31.800771302372727 35.0 32.0 37.0 18.0 37.0 7 31.791799039663807 35.0 32.0 37.0 18.0 37.0 8 31.716700083604 35.0 32.0 37.0 18.0 37.0 9 32.83269915138243 37.0 32.0 39.0 18.0 39.0 10-11 31.9600215668721 35.0 30.0 39.0 16.0 39.0 12-13 31.718378452364217 35.0 30.0 39.0 17.0 39.0 14-15 32.55279167067423 36.0 30.0 39.0 17.0 40.0 16-17 32.47436409171284 36.0 30.0 39.0 17.0 40.0 18-19 32.44360336931511 36.0 30.0 39.0 17.5 40.0 20-21 32.352787231523884 36.0 30.0 39.0 17.5 40.0 22-23 32.546533023579286 36.5 30.0 39.0 17.0 40.0 24-25 32.63998120759686 37.0 30.0 39.0 17.0 41.0 26-27 32.74313984803308 37.0 30.5 39.0 17.0 41.0 28-29 32.345490563106225 36.5 30.0 39.0 16.0 41.0 30-31 32.050972358890505 36.0 30.0 39.0 15.5 40.5 32-33 31.917770288766732 36.0 30.0 39.0 15.0 40.0 34-35 31.49350977722864 35.5 29.5 39.0 9.5 40.0 36-37 31.141521407802546 35.0 28.5 39.0 9.0 40.0 38-39 30.76978788259927 35.0 27.0 39.0 9.0 40.0 40-41 30.62199598996752 35.0 27.5 39.0 9.0 40.0 42-43 30.370014279266947 35.0 27.0 38.5 8.5 40.0 44-45 30.010812290527593 35.0 26.5 38.0 7.5 40.0 46-47 29.423773499752148 34.5 24.5 38.0 2.0 40.0 48-49 29.092254977397324 34.0 24.0 38.0 2.0 40.0 50-51 28.43217829107509 33.5 24.0 38.0 2.0 39.5 52-53 28.54961675335341 34.0 24.0 38.0 2.0 39.5 54-55 28.706351869252224 34.0 24.0 38.0 2.0 40.0 56-57 28.28620164100591 34.0 23.0 38.0 2.0 40.0 58-59 27.92296058034492 34.0 23.0 38.0 2.0 40.0 60-61 27.3334083056503 33.0 21.0 38.0 2.0 40.0 62-63 26.78431629686078 32.5 20.0 37.0 2.0 39.5 64-65 26.273655122409572 32.0 18.5 37.0 2.0 39.0 66-67 25.9237775689733 31.5 17.5 36.0 2.0 39.0 68-69 25.241347726045234 31.0 13.0 36.0 2.0 39.0 70-71 24.628393915404594 31.0 9.5 35.0 2.0 38.0 72-73 24.16513879743417 30.0 8.0 35.0 2.0 38.0 74-75 23.43499733650979 29.5 6.5 34.5 2.0 37.0 76-77 22.647714207500684 29.0 2.0 34.0 2.0 36.0 78-79 22.15117156576231 29.0 2.0 34.0 2.0 36.0 80-81 21.599920650187556 28.5 2.0 34.0 2.0 35.5 82-83 20.86334353104816 27.0 2.0 33.5 2.0 35.0 84-85 20.16492442346535 26.0 2.0 33.0 2.0 35.0 86-87 19.37926472873092 26.0 2.0 32.5 2.0 35.0 88-89 18.57880990078499 25.0 2.0 32.0 2.0 34.0 90-91 17.765118451328416 22.5 2.0 32.0 2.0 34.0 92-93 16.927179437855592 19.0 2.0 31.0 2.0 34.0 94-95 15.846225242488588 11.0 2.0 31.0 2.0 34.0 96-97 14.722543115247742 2.0 2.0 30.5 2.0 34.0 98-99 13.664836380316807 2.0 2.0 30.0 2.0 34.0 100-101 12.230771635309 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.7710201509175256 1101 2 3.732497625091394 1101 3 3.3724156959478364 1101 4 3.1759852073797 1101 5 2.459068365401361 1101 6 2.738288349823044 1101 7 2.312294902547297 1101 8 2.304228851068739 1101 9 2.5974233101889936 1101 10-11 3.4561061474145554 1101 12-13 3.4224157889054716 1101 14-15 3.0123387682083873 1101 16-17 2.786410665550278 1101 18-19 2.924289053341141 1101 20-21 2.838632584429135 1101 22-23 2.942951843601236 1101 24-25 2.9745686971548224 1101 26-27 2.7679160569720977 1101 28-29 3.115215140747914 1101 30-31 3.1610510195224073 1101 32-33 2.9286674035430202 1101 34-35 3.087979249416289 1101 36-37 3.071839063498203 1101 38-39 3.091931343457322 1101 40-41 2.797566121444632 1101 42-43 3.0103819087093413 1101 44-45 2.9603850947912242 1101 46-47 2.9480440442664566 1101 48-49 2.6480054867558955 1101 50-51 2.789254104923838 1101 52-53 2.72926885698028 1101 54-55 2.5629526046749085 1101 56-57 2.7644093258757607 1101 58-59 2.665810315012699 1101 60-61 2.677431282093387 1101 62-63 2.9697086617704294 1101 64-65 2.9027957486200258 1101 66-67 2.518336839173319 1101 68-69 2.6425045505995044 1101 70-71 2.452705462255345 1101 72-73 2.495058908715265 1101 74-75 2.427102278648519 1101 76-77 2.2840862101978345 1101 78-79 2.076352290534814 1101 80-81 2.082324308112785 1101 82-83 2.05874712813025 1101 84-85 1.853183186353995 1101 86-87 2.102726292746155 1101 88-89 2.038054628805387 1101 90-91 1.71126814088694 1101 92-93 1.579606516354385 1101 94-95 1.6110167413505252 1101 96-97 1.444520759131418 1101 98-99 1.3956092355761545 1101 100-101 1.0651129847201446 1102 1 3.5352043657076706 1102 2 2.589192982957936 1102 3 2.8027928534334556 1102 4 2.99018290324722 1102 5 1.966207440743684 1102 6 2.4176467410804463 1102 7 2.4665581182406378 1102 8 2.45371647011255 1102 9 2.4912682218955737 1102 10-11 2.013175480158605 1102 12-13 3.0133217836315858 1102 14-15 3.0556119737959264 1102 16-17 2.9422285479335883 1102 18-19 2.9434283931463057 1102 20-21 3.036986240096212 1102 22-23 2.939225886478958 1102 24-25 3.05568619793047 1102 26-27 2.75269228319479 1102 28-29 3.0034724854083734 1102 30-31 3.0839531012092074 1102 32-33 2.737129614256528 1102 34-35 3.063802166279256 1102 36-37 2.9084641976378265 1102 38-39 3.1532053405383316 1102 40-41 2.8586480355449524 1102 42-43 2.6338711042127585 1102 44-45 2.5508831829628384 1102 46-47 2.5506572045141986 1102 48-49 2.4844782810363384 1102 50-51 2.7191390793813817 1102 52-53 2.903330569257136 1102 54-55 2.7020624261319597 1102 56-57 2.826167978479713 1102 58-59 2.6974491560548515 1102 60-61 2.95335719417697 1102 62-63 2.8938036687815334 1102 64-65 2.809833692194058 1102 66-67 2.6900832382622717 1102 68-69 2.6722629366350255 1102 70-71 2.7191365057286347 1102 72-73 2.664453041409285 1102 74-75 2.6575318970545467 1102 76-77 2.5613983413040273 1102 78-79 2.3642683075812947 1102 80-81 2.3405111016261593 1102 82-83 2.342594870810437 1102 84-85 2.304510232869447 1102 86-87 2.0744575631289806 1102 88-89 1.9951662734978761 1102 90-91 1.7233338367405722 1102 92-93 1.4861729117395903 1102 94-95 1.3711002555984066 1102 96-97 1.3757117820498816 1102 98-99 0.9599973036277483 1102 100-101 0.7624444788856959 1103 1 3.8037384616206005 1103 2 3.629379147449246 1103 3 3.1834899314068252 1103 4 2.8756844091346387 1103 5 2.460595762217036 1103 6 2.723467201821485 1103 7 2.4646480277865557 1103 8 2.3439480339161243 1103 9 2.689401871084307 1103 10-11 3.407220607201051 1103 12-13 2.5655519486909633 1103 14-15 3.2103248887973237 1103 16-17 2.6217923868036337 1103 18-19 2.609255736263428 1103 20-21 2.873142307007356 1103 22-23 2.939558293058475 1103 24-25 2.9847489846961395 1103 26-27 2.720813814695738 1103 28-29 2.2390500104588753 1103 30-31 2.8929100745939884 1103 32-33 2.766464061599205 1103 34-35 2.618725145011961 1103 36-37 3.073197471951353 1103 38-39 3.2982834542462776 1103 40-41 3.3100782819491457 1103 42-43 3.2265095983757632 1103 44-45 3.123057952878032 1103 46-47 2.8731728894408697 1103 48-49 2.749194979663894 1103 50-51 2.7831630340837457 1103 52-53 2.750940561223498 1103 54-55 2.9794898817704656 1103 56-57 3.056007301469034 1103 58-59 3.095978331783172 1103 60-61 3.1077700556487713 1103 62-63 3.148683149468866 1103 64-65 3.0433219770286293 1103 66-67 2.8317579791857703 1103 68-69 2.89933570620736 1103 70-71 2.7585472999665974 1103 72-73 2.6636400659875186 1103 74-75 2.6366534655304044 1103 76-77 2.605664666568984 1103 78-79 2.422182735444917 1103 80-81 2.395618849887498 1103 82-83 2.637930684964431 1103 84-85 2.3720830965597486 1103 86-87 2.3427005341137566 1103 88-89 2.300627331770343 1103 90-91 2.1449232265368394 1103 92-93 1.9570971312327679 1103 94-95 2.099464626150912 1103 96-97 2.1443195126641026 1103 98-99 1.94224425025614 1103 100-101 1.7302922174495237 1104 1 3.64297784207103 1104 2 3.490606518335049 1104 3 2.6897896782255124 1104 4 2.4772736183153548 1104 5 1.6085908930273796 1104 6 2.063760159905918 1104 7 2.3011632269393054 1104 8 2.261632665975828 1104 9 2.232141317725933 1104 10-11 3.036806312385469 1104 12-13 3.0360962988589257 1104 14-15 2.9589739679123426 1104 16-17 2.8072979634116635 1104 18-19 2.6229342497747012 1104 20-21 2.3566388774761577 1104 22-23 2.2233873191378066 1104 24-25 2.3050109363434714 1104 26-27 2.363298011548345 1104 28-29 2.103057463173535 1104 30-31 1.814911561827362 1104 32-33 2.0765864532524994 1104 34-35 2.533305306683946 1104 36-37 2.731981095009054 1104 38-39 2.708959783448176 1104 40-41 2.5843388539237893 1104 42-43 2.5157928822035984 1104 44-45 2.455012837374401 1104 46-47 2.442577354864248 1104 48-49 2.1741765185264015 1104 50-51 2.0154260696118733 1104 52-53 2.3802132027485428 1104 54-55 2.20151122636182 1104 56-57 2.3700118066071276 1104 58-59 2.577360300474737 1104 60-61 2.7067542793915536 1104 62-63 2.662768169988137 1104 64-65 2.7581950347308 1104 66-67 2.5813712778238873 1104 68-69 2.317219997796112 1104 70-71 2.2773326191736274 1104 72-73 2.194186008881065 1104 74-75 2.271360197174758 1104 76-77 2.4010654128746864 1104 78-79 1.9764090948465487 1104 80-81 2.1055938627120447 1104 82-83 2.219794763417543 1104 84-85 2.016126354573018 1104 86-87 2.1547712738830818 1104 88-89 2.184097374049969 1104 90-91 1.98577751696725 1104 92-93 1.9140323652186346 1104 94-95 2.073390518904631 1104 96-97 2.230402710857083 1104 98-99 2.141680849435369 1104 100-101 1.727479143447768 1105 1 3.479318566779238 1105 2 3.1868442528411265 1105 3 2.831335112302341 1105 4 2.749648566566048 1105 5 -0.07566351746628897 1105 6 1.7728259123953265 1105 7 2.010618279487556 1105 8 2.091703916602267 1105 9 2.605064627066959 1105 10-11 2.640043821736665 1105 12-13 3.1236862700245744 1105 14-15 2.883095682285216 1105 16-17 2.753278585565454 1105 18-19 2.8815838288803306 1105 20-21 2.82723473898411 1105 22-23 2.587637444425603 1105 24-25 2.0770343524514843 1105 26-27 2.4375123299464008 1105 28-29 2.5390931393281804 1105 30-31 2.549449231692197 1105 32-33 2.3524571126657854 1105 34-35 2.703811469391038 1105 36-37 2.7128193248558716 1105 38-39 2.812194024422002 1105 40-41 2.645750938204344 1105 42-43 2.693405223596514 1105 44-45 2.5696144948048705 1105 46-47 2.821564230513385 1105 48-49 2.7007676735709616 1105 50-51 2.7243690763737796 1105 52-53 2.9166911993058484 1105 54-55 2.7873595155478483 1105 56-57 2.999732977703051 1105 58-59 2.951444973237532 1105 60-61 2.981900085337095 1105 62-63 2.7572878274621893 1105 64-65 2.5797474413459973 1105 66-67 2.570623400453403 1105 68-69 2.7639094946838902 1105 70-71 2.760951875000419 1105 72-73 2.653899626393759 1105 74-75 2.6826041415978175 1105 76-77 2.679804254997535 1105 78-79 2.4812662667559344 1105 80-81 2.630832998988854 1105 82-83 2.7113993914398833 1105 84-85 2.457602063266176 1105 86-87 2.4805478766590205 1105 88-89 2.4041975316583866 1105 90-91 2.3277518347842623 1105 92-93 2.556026656452847 1105 94-95 2.5489183618482087 1105 96-97 2.540350198927504 1105 98-99 2.354318938378812 1105 100-101 2.0270139444035884 1106 1 3.2140080238987814 1106 2 2.563118162152641 1106 3 2.239535224629744 1106 4 1.4203544277251403 1106 5 1.8385611585327375 1106 6 2.2430296912587053 1106 7 2.081124282645437 1106 8 0.5459828488561307 1106 9 2.043017301200919 1106 10-11 0.9384393413568652 1106 12-13 1.7223994854494542 1106 14-15 1.6675004000361255 1106 16-17 1.8689788833390395 1106 18-19 2.093075028134308 1106 20-21 2.0267799189728777 1106 22-23 2.3892786961343617 1106 24-25 2.501000463486072 1106 26-27 2.5116702453193582 1106 28-29 2.1648862661627746 1106 30-31 2.222317819228099 1106 32-33 2.412017340287729 1106 34-35 2.5805573657319236 1106 36-37 1.703376511160414 1106 38-39 2.6351727140062913 1106 40-41 1.8588600759325828 1106 42-43 2.1075277452407306 1106 44-45 2.050431093827836 1106 46-47 2.5197444206222137 1106 48-49 2.268177255310338 1106 50-51 2.1763086315148783 1106 52-53 2.2363816631332476 1106 54-55 2.2238055189176684 1106 56-57 2.366299468107531 1106 58-59 2.467222416623464 1106 60-61 2.6543978864752056 1106 62-63 2.77098594226927 1106 64-65 2.781643972972521 1106 66-67 2.2531900816025363 1106 68-69 2.5625006335353966 1106 70-71 2.5647357189289117 1106 72-73 2.7409386336738457 1106 74-75 2.81607166732185 1106 76-77 3.0109743674309044 1106 78-79 2.568951262570149 1106 80-81 2.584787347436933 1106 82-83 2.8342538850658343 1106 84-85 2.749400043492315 1106 86-87 2.965683570280735 1106 88-89 3.048794371655422 1106 90-91 2.7974239659318023 1106 92-93 2.793220060926867 1106 94-95 2.9311017522283187 1106 96-97 2.8552120680130972 1106 98-99 2.657319351658879 1106 100-101 2.122786619173368 1107 1 3.082133596432879 1107 2 3.4071318299114566 1107 3 0.014788151227360657 1107 4 0.9157404351322533 1107 5 1.465367050505737 1107 6 1.9499966514749616 1107 7 2.0869783770721178 1107 8 1.9397691889652684 1107 9 2.2582166133170105 1107 10-11 1.7468808684141983 1107 12-13 2.38448370626919 1107 14-15 2.754818551966302 1107 16-17 1.1464931721041651 1107 18-19 0.2738612614640772 1107 20-21 -1.3833064037143643 1107 22-23 0.4192750993557226 1107 24-25 1.1510254161378484 1107 26-27 1.1058838714904269 1107 28-29 2.0240981220443572 1107 30-31 2.66884810803818 1107 32-33 1.3305603077425054 1107 34-35 0.7820462072882002 1107 36-37 0.9916456176419146 1107 38-39 2.069327330527866 1107 40-41 2.2982618304861546 1107 42-43 1.3435832030713115 1107 44-45 1.064891717531733 1107 46-47 1.603781917039484 1107 48-49 1.256600742274827 1107 50-51 1.597188043891169 1107 52-53 1.2088998577751724 1107 54-55 1.6945600556357228 1107 56-57 2.264231994788325 1107 58-59 2.2689018312477387 1107 60-61 2.1817468920221117 1107 62-63 2.020170204114752 1107 64-65 1.2623474241940187 1107 66-67 1.8606327719929574 1107 68-69 2.095406100076925 1107 70-71 0.6526931420618141 1107 72-73 1.6565915894952887 1107 74-75 2.0537275230460708 1107 76-77 2.2806915707107187 1107 78-79 2.1489373250529376 1107 80-81 2.0388390061700825 1107 82-83 1.837069432141483 1107 84-85 2.113834646750771 1107 86-87 2.3763974746401395 1107 88-89 2.2930318264849383 1107 90-91 2.3290374021479927 1107 92-93 2.569196783025138 1107 94-95 2.540929949848824 1107 96-97 2.3721097902406267 1107 98-99 2.3151735922046885 1107 100-101 2.0580146493188707 1108 1 2.258992936527136 1108 2 2.398645319421604 1108 3 1.7789959073896071 1108 4 1.770899374581564 1108 5 1.886359753380507 1108 6 0.03429973269268061 1108 7 0.5781279756737199 1108 8 -0.2644693219239116 1108 9 1.2308726425808558 1108 10-11 2.683737260650684 1108 12-13 1.7572613346658095 1108 14-15 2.0859479770492797 1108 16-17 0.1440146876986148 1108 18-19 0.2533820674065055 1108 20-21 1.0619026446078976 1108 22-23 -0.638940205865282 1108 24-25 1.152604003388781 1108 26-27 1.3405706686665084 1108 28-29 2.0408707734587566 1108 30-31 0.7255259123644642 1108 32-33 0.9816082923684384 1108 34-35 1.2959353381693184 1108 36-37 0.2674310734014469 1108 38-39 1.69685991000701 1108 40-41 0.39303398603886563 1108 42-43 1.5901913418899838 1108 44-45 2.305716627965829 1108 46-47 2.2420252033640615 1108 48-49 2.0377202430602956 1108 50-51 2.2182786594377752 1108 52-53 1.3442728088727556 1108 54-55 1.7918642020378286 1108 56-57 2.5535468109286477 1108 58-59 2.5406296855840083 1108 60-61 1.885352331620222 1108 62-63 2.0534356134996763 1108 64-65 1.4721500754765735 1108 66-67 1.9333213642543114 1108 68-69 2.5789143427769723 1108 70-71 2.737751729557626 1108 72-73 2.5677853730000955 1108 74-75 2.8156337957671767 1108 76-77 2.8360037540279173 1108 78-79 2.6443136972666004 1108 80-81 2.74096551353313 1108 82-83 2.799089275085649 1108 84-85 2.702521665722543 1108 86-87 2.433460772586624 1108 88-89 2.5631846842117163 1108 90-91 2.5171405710479995 1108 92-93 2.5329174127553706 1108 94-95 2.7518716585515044 1108 96-97 2.659128479470491 1108 98-99 2.505710745101501 1108 100-101 2.2019594033563994 1109 1 1.4496479617468587 1109 2 1.3491785565962147 1109 3 0.6042307209431108 1109 4 2.0479579124228486 1109 5 1.922809370501053 1109 6 0.3801746600372553 1109 7 0.45991150542051074 1109 8 -2.322158703894445 1109 9 -0.540687488803556 1109 10-11 -0.48740515080721636 1109 12-13 0.7609725517855814 1109 14-15 0.9315944542852108 1109 16-17 -0.11152845792040722 1109 18-19 1.256570433663967 1109 20-21 -0.5991477333953519 1109 22-23 -1.1907566280391322 1109 24-25 -0.24303318468050605 1109 26-27 -2.6897643118481867 1109 28-29 -1.7631439019616693 1109 30-31 -0.21527850752342204 1109 32-33 -2.3289943161075755 1109 34-35 0.7257462365804557 1109 36-37 1.8522224523721391 1109 38-39 2.3487016396742213 1109 40-41 -0.16463119777447943 1109 42-43 -0.007869404383626488 1109 44-45 -0.9150007980231614 1109 46-47 -1.7306912829806187 1109 48-49 1.0027490297244661 1109 50-51 1.1915499357741481 1109 52-53 0.4366672244500762 1109 54-55 1.0191938811446626 1109 56-57 0.306544700508006 1109 58-59 -0.11947934143795891 1109 60-61 0.8146829240398752 1109 62-63 -0.866987253259051 1109 64-65 1.3803475699859682 1109 66-67 0.0524622406835249 1109 68-69 -0.11296448748045052 1109 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54-55 -0.683031204842873 2312 56-57 0.24935590379656247 2312 58-59 -0.18297289647445325 2312 60-61 0.32593513525450035 2312 62-63 -0.04067238226273062 2312 64-65 -1.4330192037735898 2312 66-67 1.48468646157529 2312 68-69 0.5698600800462437 2312 70-71 -2.244811968998718 2312 72-73 -2.4435829563829436 2312 74-75 -0.532633235404262 2312 76-77 0.038024466158923076 2312 78-79 -0.10499387832773621 2312 80-81 -1.5574166598490429 2312 82-83 0.05078386317729766 2312 84-85 1.7449144241079466 2312 86-87 2.0792873724573404 2312 88-89 2.382057906577586 2312 90-91 2.8700020682109653 2312 92-93 3.0546979198299944 2312 94-95 2.3853041982340457 2312 96-97 1.6068229418708366 2312 98-99 1.191853952384415 2312 100-101 1.9592304207377111 2313 1 -7.518055102239654 2313 2 -4.148067516353734 2313 3 -1.6048611209986703 2313 4 0.41935118318961884 2313 5 0.90899120179321 2313 6 -2.4252879886137926 2313 7 -0.7230443762798835 2313 8 -1.8307324858433276 2313 9 0.7943694756089386 2313 10-11 -1.150981622896321 2313 12-13 -2.160399780260928 2313 14-15 -3.594264171912876 2313 16-17 -3.4726243156591394 2313 18-19 -0.7780491749622342 2313 20-21 -2.5227089243786693 2313 22-23 -1.1734835704867734 2313 24-25 -2.1702101182534577 2313 26-27 -1.993284928860966 2313 28-29 -2.4926447561505185 2313 30-31 -4.27741450659158 2313 32-33 -5.344864063400795 2313 34-35 -4.094353409541302 2313 36-37 -1.5708124972640007 2313 38-39 -1.201002860902637 2313 40-41 -3.189619688411682 2313 42-43 -2.0197989282565416 2313 44-45 -2.3579857720646977 2313 46-47 -0.7946549133941794 2313 48-49 -1.8585423485147956 2313 50-51 -2.6151357560078914 2313 52-53 0.06717230520974837 2313 54-55 -1.3725536283956252 2313 56-57 -1.746736022557311 2313 58-59 -0.5220324786406536 2313 60-61 -1.223321546584902 2313 62-63 -3.5017813049984916 2313 64-65 -5.232272238320743 2313 66-67 -3.639392597131529 2313 68-69 -2.2587354206540375 2313 70-71 -1.1037875904973191 2313 72-73 -1.0839526459254252 2313 74-75 -1.92336765417177 2313 76-77 -1.8943219098914987 2313 78-79 -2.209607338295058 2313 80-81 -3.02009027096015 2313 82-83 -0.9302228624296127 2313 84-85 -0.7367907579648829 2313 86-87 -2.254749888280287 2313 88-89 -3.1732459858127022 2313 90-91 -2.8966405263268484 2313 92-93 -1.1915417218600162 2313 94-95 -0.4982833513691318 2313 96-97 -0.6503786366772495 2313 98-99 -0.5309646021207204 2313 100-101 -0.14988926880477216 2314 1 -7.421152762216053 2314 2 -3.327052883753442 2314 3 -25.259809037665338 2314 4 -13.486259248539557 2314 5 -6.478126077101706 2314 6 -5.099405694279806 2314 7 -4.343907089351632 2314 8 -3.227040641250767 2314 9 -3.153482665464015 2314 10-11 -14.395950293661244 2314 12-13 -13.087813488556602 2314 14-15 -4.507430374152168 2314 16-17 -2.6714021734564994 2314 18-19 -2.8802581076579195 2314 20-21 -3.9627985776899095 2314 22-23 -3.9076268471160347 2314 24-25 -4.873540364556867 2314 26-27 -3.6819878702866333 2314 28-29 -3.446011187779277 2314 30-31 -3.5758225287899528 2314 32-33 -3.8686607689475636 2314 34-35 -3.0540238347747746 2314 36-37 -3.736332510788877 2314 38-39 -0.7585615536964809 2314 40-41 -2.005356068608247 2314 42-43 -4.261746957892026 2314 44-45 -5.221359887926777 2314 46-47 -4.604589285569958 2314 48-49 -3.051626067824934 2314 50-51 -2.6370573948716753 2314 52-53 -1.0775395772273626 2314 54-55 -3.223770651698583 2314 56-57 -2.962088818692525 2314 58-59 -1.1427886950638104 2314 60-61 -3.8783141800763907 2314 62-63 -5.096396810910523 2314 64-65 -4.497209226823028 2314 66-67 -5.486217369154172 2314 68-69 -4.933007281689836 2314 70-71 -5.5798249619952855 2314 72-73 -4.789229152803973 2314 74-75 -3.9121621670251123 2314 76-77 -3.8179861712636907 2314 78-79 -4.833182774009522 2314 80-81 -7.474397128795168 2314 82-83 -6.860277609159793 2314 84-85 -5.263377656092111 2314 86-87 -4.65012761992913 2314 88-89 -3.743992513590481 2314 90-91 -5.790074645107287 2314 92-93 -5.785837149447001 2314 94-95 -6.242881343191442 2314 96-97 -5.688749692967102 2314 98-99 -6.462566197962213 2314 100-101 -5.502296681442218 2315 1 -24.720746074461875 2315 2 -24.850324460798177 2315 3 -13.341609618497865 2315 4 -29.128825900143713 2315 5 -15.625913791080837 2315 6 -11.958088808098573 2315 7 -8.922052204746056 2315 8 -8.048992156301537 2315 9 -5.387710009036649 2315 10-11 -29.578281970118542 2315 12-13 -29.321207071927592 2315 14-15 -23.902364231071743 2315 16-17 -14.072089785030975 2315 18-19 -8.920256506628363 2315 20-21 -6.661633106910671 2315 22-23 -3.323323782719214 2315 24-25 -5.192399945953255 2315 26-27 -5.210943977142669 2315 28-29 -17.646665794523113 2315 30-31 -16.620992450081417 2315 32-33 -9.686252540530514 2315 34-35 -7.09565738360552 2315 36-37 -4.86622008001919 2315 38-39 -5.183962543797659 2315 40-41 -8.860933141340045 2315 42-43 -8.259118819753795 2315 44-45 -5.361429633323706 2315 46-47 -4.034083837038903 2315 48-49 -4.841210648772947 2315 50-51 -3.3270997127950253 2315 52-53 -7.29910260201839 2315 54-55 -7.047688458515559 2315 56-57 -7.924972491646802 2315 58-59 -7.7877238643026985 2315 60-61 -5.458854934371288 2315 62-63 -3.954814289428949 2315 64-65 -5.186639238428302 2315 66-67 -7.5748974558897295 2315 68-69 -7.2974227156147755 2315 70-71 -8.12327913514882 2315 72-73 -7.379541108601558 2315 74-75 -8.002557345738943 2315 76-77 -7.819087207164811 2315 78-79 -7.63512535654019 2315 80-81 -8.715263277037383 2315 82-83 -7.928330660980219 2315 84-85 -7.082160904248628 2315 86-87 -7.157460860233606 2315 88-89 -6.474389415817004 2315 90-91 -5.92176413811022 2315 92-93 -5.888968058984894 2315 94-95 -5.94496747158264 2315 96-97 -6.069084981802019 2315 98-99 -5.894322531762004 2315 100-101 -5.627636626823488 2316 1 -1.1086634035556742 2316 2 1.6928657882744247 2316 3 1.8965237657262897 2316 4 2.0810310155955527 2316 5 -1.843199637943588 2316 6 -2.7214087877217246 2316 7 -2.669835606848686 2316 8 -4.238802090374328 2316 9 -3.8614878983091003 2316 10-11 -3.2369995111890226 2316 12-13 -3.5424577343547234 2316 14-15 -1.8608303704643667 2316 16-17 -2.2855542032673313 2316 18-19 -4.070280284748488 2316 20-21 -1.676924721057702 2316 22-23 -2.1447055967409696 2316 24-25 -2.282493242370222 2316 26-27 -2.2019919124990146 2316 28-29 -1.3180089530680235 2316 30-31 1.1340986839887073 2316 32-33 -1.048106023191469 2316 34-35 -6.754148429745332 2316 36-37 -2.698352836316289 2316 38-39 -0.7813756279711761 2316 40-41 -0.5628073291774456 2316 42-43 -0.3560365905511844 2316 44-45 0.3240971610522436 2316 46-47 1.155862814811968 2316 48-49 1.4480247884751343 2316 50-51 1.6903887482758044 2316 52-53 1.608180684912984 2316 54-55 -0.5009709933708955 2316 56-57 0.6536635728101139 2316 58-59 -2.701192797047298 2316 60-61 -2.7847595930872906 2316 62-63 -4.553763204177081 2316 64-65 -4.53800444111177 2316 66-67 -2.299223498805258 2316 68-69 -1.8669380626342544 2316 70-71 -2.698252804792496 2316 72-73 -2.84492547537252 2316 74-75 -2.420973256097227 2316 76-77 -2.101009525816245 2316 78-79 -2.4878325994017523 2316 80-81 -2.4455106361783123 2316 82-83 -3.453461979303839 2316 84-85 -2.7095042803107567 2316 86-87 -2.100192527610794 2316 88-89 -3.374623144221111 2316 90-91 -3.7325290343201374 2316 92-93 -4.305460557975543 2316 94-95 -5.538813196471587 2316 96-97 -5.4779298781761 2316 98-99 -5.727008187065961 2316 100-101 -5.498992508073453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33258.0 3 11872.0 4 10723.0 5 11322.0 6 13309.0 7 16281.0 8 19957.0 9 24271.0 10 28824.0 11 33666.0 12 37848.0 13 42779.0 14 46756.0 15 50627.0 16 54399.0 17 55111.0 18 52100.0 19 52849.0 20 56562.0 21 61768.0 22 67663.0 23 73963.0 24 81840.0 25 89159.0 26 97558.0 27 107115.0 28 116192.0 29 126916.0 30 136586.0 31 146909.0 32 157955.0 33 167520.0 34 173903.0 35 173082.0 36 153720.0 37 98372.0 38 20302.0 39 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55633140365108 19.4640890964214 14.990762815629502 21.988816684298023 2 15.557225584164897 20.622740537149888 40.8829821727813 22.937051705903922 3 19.39792448284813 25.839833719590427 29.732894351060075 25.029347446501372 4 12.57007044355561 17.323891110978447 37.87408214434959 32.23195630111635 5 13.417437948823597 38.00040090986531 33.972405396202326 14.609755745108771 6 30.55109084062652 36.57336492389127 18.15631402021692 14.719230215265288 7 27.923164191571892 33.60568755896462 20.868366526311735 17.60278172315175 8 26.14852477603827 34.56884890725043 19.947470407206175 19.33515590950513 9 26.91205134053539 17.18102485566769 20.784963372621736 35.12196043117519 10-11 24.58652877928243 27.21794783286547 27.242797208216885 20.95272617963521 12-13 26.195851207607106 24.898524583222954 27.700924697428462 21.204699511741474 14-15 23.321471100280817 25.90872282093034 26.88627441067835 23.883531668110493 16-17 21.499133533895108 29.20200887588588 27.404394846410295 21.894462743808717 18-19 21.275129627397714 27.423727326197834 30.602970354403443 20.69817269200101 20-21 22.629166109574296 26.335623553951788 30.551408483828517 20.483801852645396 22-23 22.006744675436572 26.50209604272615 30.3250727604829 21.166086521354373 24-25 21.51206169923328 26.658474382874296 30.840058563591583 20.98940535430084 26-27 21.572691222181202 26.758130102007264 30.68506999697892 20.98410867883261 28-29 21.190564335840264 26.866090705112533 30.866818853998524 21.07652610504868 30-31 21.393814229747015 26.867470861762538 30.800651172718634 20.93806373577181 32-33 21.29708774843209 26.831369811598986 30.498425885066823 21.373116554902097 34-35 21.770737634472333 26.853606673976827 30.362389977180452 21.013265714370387 36-37 21.821991285804927 26.691398742023747 30.401691574685348 21.084918397485975 38-39 21.574297895762502 26.831929709817526 30.549837502049677 21.043934892370295 40-41 21.666863884615832 26.779993840874187 30.49287635804928 21.0602659164607 42-43 21.520893085711577 27.010846538115103 30.18764222053665 21.280618155636674 44-45 21.603584427061506 27.15947229926721 29.977889934132783 21.259053339538497 46-47 21.67598839386622 27.285749370186107 29.33947422717455 21.69878800877313 48-49 21.787905394891858 27.33256274034721 29.30144634375857 21.578085521002365 50-51 22.04280008944552 27.5753798053578 29.028498995590528 21.35332110960615 52-53 21.783638761378853 27.774251988653155 28.994376793558637 21.44773245640936 54-55 21.630590852517283 27.685665045158924 28.98900446910584 21.694739633217953 56-57 21.672542464308048 27.98481999164199 28.702363804493764 21.640273739556196 58-59 21.691642080191727 27.661886426228538 28.92301053282734 21.72346096075239 60-61 21.68034662097691 27.663906233537343 29.054356454545438 21.601390690940306 62-63 21.762683516419212 27.518194703231252 29.052990279245318 21.666131501104218 64-65 21.746829442622587 27.48931111551402 29.026232901951126 21.737626539912267 66-67 21.510753441766102 27.710015270403915 28.780887757254092 21.998343530575895 68-69 21.622767590567012 27.716454955576314 28.80226853810568 21.858508915750992 70-71 21.575121957452527 27.66198540417949 28.64955234582565 22.113340292542333 72-73 21.591886068320633 27.692762736544292 28.470862736803582 22.244488458331496 74-75 21.529476594227397 27.86493232261022 28.378233951220416 22.227357131941968 76-77 21.742865490261394 27.73192425056176 28.355536749341343 22.1696735098355 78-79 21.52470072984587 27.72997462815284 28.442387195322468 22.302937446678825 80-81 21.39033618956506 27.923827315930154 28.39029617317316 22.29554032133163 82-83 21.621577562589348 27.97698031719309 28.120418001457164 22.2810241187604 84-85 21.604690047134227 27.785558260047488 28.092402122901657 22.517349569916632 86-87 21.469860308426135 27.737884833449222 28.29112777542956 22.501127082695085 88-89 21.768177407474017 27.53722830178859 28.18612011629451 22.50847417444288 90-91 21.496098842415165 27.727263128920654 28.273195503916348 22.503442524747836 92-93 21.49008136128649 27.70873370662818 28.17497566273665 22.626209269348678 94-95 21.332560235220473 27.68636306444084 28.104358609305457 22.87671809103323 96-97 21.546688884511376 27.408634456845416 27.8718994978562 23.17277716078701 98-99 21.384860240834783 27.455791754219444 27.784039380818587 23.375308624127182 100-101 21.535125065883427 27.50533602447313 27.907190159727563 23.05234874991588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4741.0 1 3223.5 2 1587.0 3 1457.5 4 1328.5 5 1303.0 6 1808.0 7 3190.5 8 4795.5 9 5495.5 10 4936.0 11 4123.5 12 3860.0 13 3772.0 14 3871.5 15 4089.0 16 4298.0 17 4684.5 18 5208.0 19 5745.0 20 6432.0 21 7428.0 22 8739.0 23 10278.0 24 12118.0 25 14806.5 26 18691.5 27 22826.0 28 26400.0 29 30491.5 30 35437.0 31 41045.0 32 47142.5 33 53608.0 34 60790.5 35 66996.0 36 73155.5 37 79206.5 38 82488.5 39 84121.5 40 85292.5 41 86871.0 42 88302.0 43 89881.5 44 92414.0 45 95052.5 46 97339.5 47 99638.0 48 103590.0 49 102344.5 50 95151.5 51 89375.5 52 85673.5 53 82526.5 54 78390.0 55 72636.0 56 66017.5 57 59311.5 58 53125.0 59 46788.5 60 39631.5 61 32752.0 62 26304.0 63 20304.0 64 15097.5 65 11326.0 66 8569.5 67 6477.5 68 4977.5 69 3810.0 70 2937.5 71 2184.0 72 1571.0 73 1156.0 74 856.5 75 638.5 76 455.5 77 320.5 78 233.0 79 172.0 80 124.0 81 91.5 82 61.5 83 33.0 84 18.0 85 16.5 86 11.5 87 6.0 88 4.5 89 2.5 90 1.5 91 1.0 92 0.5 93 2.5 94 2.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.045397710878138 2 2.3383964309231215 3 0.9549722183174141 4 0.853056724942846 5 0.1612521363411043 6 0.08926391488669068 7 0.09055866707112259 8 0.09792025806260682 9 0.09277824224443441 10-11 1.6722834249524638 12-13 1.2705958079623558 14-15 0.4819992453444411 16-17 0.0853241689540622 18-19 0.09242680950866004 20-21 0.11797042046152366 22-23 0.13350744667470646 24-25 0.14545615969103512 26-27 0.14255221550595218 28-29 0.5256508904195737 30-31 0.17401469358764732 32-33 0.15696095767270143 34-35 0.1398332359186452 36-37 0.12899431048897242 38-39 0.39673056576971166 40-41 0.11695311517375573 42-43 0.11793342754196846 44-45 0.12276100354392169 46-47 0.13365541835292724 48-49 0.14797167822078852 50-51 0.16167755491598906 52-53 0.1793601704633733 54-55 0.17776947492249984 56-57 0.14841559325545092 58-59 0.13187975821427778 60-61 0.11022040381470986 62-63 0.11545490193177026 64-65 0.11042386487226344 66-67 0.1072239773307389 68-69 0.10367265705343996 70-71 0.11839583903640843 72-73 0.1319537440533882 74-75 0.14177536419529302 76-77 0.16086371068577474 78-79 0.1613816115595475 80-81 0.16003136999578282 82-83 0.15309519757918333 84-85 0.14062858368908193 86-87 0.1395742854817588 88-89 0.13667034129667582 90-91 0.14401343582838244 92-93 0.13524611389380073 94-95 0.18442820044243532 96-97 0.18823847115662062 98-99 0.20573612210622888 100-101 0.23015144901265897 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2703220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.12318088072312 #Duplication Level Percentage of deduplicated Percentage of total 1 79.170393821673 49.9748708960355 2 11.612097950430467 14.659851186593931 3 4.1502891444689185 7.859383571208394 4 1.8846502413974668 4.758604723385231 5 1.023709700509968 3.23099062973208 6 0.5799351098628757 2.1964409303373804 7 0.36493497838250644 1.6125099655099215 8 0.2384240083328376 1.2040065443440597 9 0.17848941495817544 1.014013766312942 >10 0.7411430341802053 7.969949600697397 >50 0.03638228943983534 1.5614383335823954 >100 0.018140929680652413 1.972333159634745 >500 7.966042121270001E-4 0.3789494052249512 >1k 4.2894072960063213E-4 0.5806276943731847 >5k 1.2255449417160918E-4 0.5720407016244774 >10k+ 6.127724708580459E-5 0.4539888914035088 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11737 0.43418589681934877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8403 0.31085150302232156 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6386 0.2362367842794889 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3673 0.13587499352623908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.699291955519713E-5 0.0 0.0 0.0 0.0 3 3.699291955519713E-5 0.0 0.0 0.0 0.0 4 3.699291955519713E-5 0.0 0.0 0.0 0.0 5 3.699291955519713E-5 0.0 0.0 0.0 0.0 6 3.699291955519713E-5 0.0 0.0 0.0 0.0 7 3.699291955519713E-5 0.0 0.0 0.0 0.0 8 3.699291955519713E-5 0.0 0.0 0.0 0.0 9 3.699291955519713E-5 0.0 0.0 0.0 0.0 10-11 3.699291955519713E-5 0.0 0.0 1.8496459777598566E-5 0.0 12-13 7.398583911039426E-5 0.0 0.0 3.699291955519713E-5 0.0 14-15 7.398583911039426E-5 0.0 0.0 3.699291955519713E-5 0.0 16-17 7.398583911039426E-5 0.0 0.0 3.699291955519713E-5 1.8496459777598566E-5 18-19 7.398583911039426E-5 0.0 0.0 9.248229888799283E-5 3.699291955519713E-5 20-21 7.398583911039426E-5 0.0 0.0 1.664681379983871E-4 3.699291955519713E-5 22-23 7.398583911039426E-5 0.0 0.0 3.6992919555197133E-4 3.699291955519713E-5 24-25 7.398583911039426E-5 0.0 0.0 7.028654715487456E-4 3.699291955519713E-5 26-27 7.398583911039426E-5 0.0 0.0 0.0011467805062111112 3.699291955519713E-5 28-29 7.398583911039426E-5 0.0 0.0 0.002571007909086201 3.699291955519713E-5 30-31 9.248229888799283E-5 0.0 0.0 0.0058633777494987455 3.699291955519713E-5 32-33 1.109787586655914E-4 0.0 0.0 0.012744060786765413 3.699291955519713E-5 34-35 1.4797167822078853E-4 0.0 0.0 0.024840745481314874 3.699291955519713E-5 36-37 1.4797167822078853E-4 0.0 0.0 0.04335570171869105 3.699291955519713E-5 38-39 1.4797167822078853E-4 0.0 0.0 0.07039752591354015 3.699291955519713E-5 40-41 1.4797167822078853E-4 0.0 0.0 0.10241489778856327 3.699291955519713E-5 42-43 1.4797167822078853E-4 0.0 0.0 0.13345195729537368 3.699291955519713E-5 44-45 1.4797167822078853E-4 0.0 0.0 0.173200849357433 3.699291955519713E-5 46-47 2.219575173311828E-4 0.0 0.0 0.22301181553850594 3.699291955519713E-5 48-49 2.219575173311828E-4 0.0 0.0 0.27587469758288263 3.699291955519713E-5 50-51 2.219575173311828E-4 0.0 0.0 0.3318264884101183 3.699291955519713E-5 52-53 2.219575173311828E-4 0.0 0.0 0.3900163508704434 3.699291955519713E-5 54-55 2.219575173311828E-4 0.0 0.0 0.4563446556329118 3.699291955519713E-5 56-57 2.219575173311828E-4 0.0 0.0 0.5329384955719475 3.699291955519713E-5 58-59 2.219575173311828E-4 0.0 0.0 0.6195574167104416 3.699291955519713E-5 60-61 2.4045397710878137E-4 0.0 0.0 0.7182545260837075 3.699291955519713E-5 62-63 2.774468966639785E-4 0.0 0.0 0.8207249132516037 3.699291955519713E-5 64-65 2.9594335644157706E-4 0.0 0.0 0.9253593862134788 3.699291955519713E-5 66-67 2.9594335644157706E-4 0.0 0.0 1.043940189847663 3.699291955519713E-5 68-69 2.9594335644157706E-4 0.0 0.0 1.1826636381796525 3.699291955519713E-5 70-71 2.9594335644157706E-4 0.0 0.0 1.3373865242192644 3.699291955519713E-5 72-73 3.144398162191756E-4 0.0 0.0 1.497713837571489 3.699291955519713E-5 74-75 3.329362759967742E-4 0.0 0.0 1.6625357906496696 3.699291955519713E-5 76-77 3.329362759967742E-4 0.0 0.0 1.8420809257108188 3.699291955519713E-5 78-79 3.329362759967742E-4 0.0 0.0 2.0463928204141726 3.699291955519713E-5 80-81 3.329362759967742E-4 0.0 0.0 2.273048438528866 3.699291955519713E-5 82-83 3.329362759967742E-4 0.0 0.0 2.508711832555249 3.699291955519713E-5 84-85 3.329362759967742E-4 0.0 0.0 2.7464283336169455 3.699291955519713E-5 86-87 3.6992919555197133E-4 0.0 0.0 3.0000517900873773 3.699291955519713E-5 88-89 3.6992919555197133E-4 0.0 0.0 3.2772582327742468 3.699291955519713E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16475 0.0 33.587666 1 GGTATCA 11015 0.0 29.877832 1 TATCAAC 20350 0.0 26.761475 2 ATCAACG 21010 0.0 26.006432 3 TCAACGC 21475 0.0 25.417397 4 CAACGCA 21575 0.0 25.166424 5 GTACATG 13505 0.0 24.814846 1 AACGCAG 21915 0.0 24.619408 6 TACATGG 13450 0.0 24.185303 2 ACGCAGA 23990 0.0 22.308693 7 AGTACTT 11645 0.0 22.129414 12-13 ACATGGG 13945 0.0 22.074451 3 CGCAGAG 24255 0.0 22.045826 8 GAGTACT 11070 0.0 21.673426 12-13 GCAGAGT 23850 0.0 20.999018 9 GTACTTT 12250 0.0 20.006453 14-15 CATGGGA 9015 0.0 19.724312 4 GTATAGG 870 0.0 19.606018 1 AGAGTAC 21980 0.0 18.45542 10-11 TACTTTT 14150 0.0 18.27578 14-15 >>END_MODULE