FastQCFastQC Report
Fri 27 May 2016
SRR939372_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939372_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2703220
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153640.5683592160460488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130020.48098194005667316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97740.3615687957324968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52850.19550757984921688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.1282174591783133No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA30740.113716234712676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27680.10239640132878568No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA27580.10202647213323371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116850.034.7790831
GTATCAA170600.029.5819851
GTACATG150050.024.4893951
TACATGG147250.024.2063352
TATCAAC215100.023.4550972
ATCAACG218050.022.98033
TCAACGC220700.022.7089964
CAACGCA223100.022.377265
ACATGGG153100.022.2224463
AACGCAG229900.021.756636
GAGTACT128300.020.96703312-13
AGTACTT133400.020.62738612-13
TCGACGA600.00454664419.95332370-71
CATGGGA100350.019.8167764
GTACTTT137200.019.57237814-15
ACGCAGA257950.019.2621127
CGCAGAG258850.019.1218768
CATGGGG50150.018.170494
GCAGAGT265950.017.8626489
TACTTTT154950.017.6362214-15
Produced by FastQC (version 0.11.5)