##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2703220 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.83847818527534 31.0 31.0 31.0 28.0 34.0 2 31.098083396837847 31.0 31.0 34.0 28.0 34.0 3 31.093072705884094 31.0 31.0 34.0 27.0 34.0 4 35.01111008353001 35.0 35.0 37.0 32.0 37.0 5 34.57311539571326 35.0 35.0 37.0 30.0 37.0 6 34.63883183758629 35.0 35.0 37.0 30.0 37.0 7 34.476449937481966 35.0 35.0 37.0 30.0 37.0 8 34.38361213663705 35.0 35.0 37.0 30.0 37.0 9 35.86907095981829 37.0 35.0 39.0 30.0 39.0 10-11 35.66591398406345 37.0 35.0 39.0 30.0 39.0 12-13 35.65431559399531 37.0 35.0 39.0 30.0 39.0 14-15 36.450335710744966 38.0 35.0 40.0 30.0 41.0 16-17 36.37097683503377 38.0 35.0 40.0 30.0 41.0 18-19 36.39039349368531 38.0 35.0 40.0 30.0 41.0 20-21 36.280090410695394 38.0 34.0 40.0 29.5 41.0 22-23 36.11428925503658 38.0 34.0 40.0 29.0 41.0 24-25 35.94739514356952 38.0 34.0 40.0 29.0 41.0 26-27 35.4574847774136 38.0 34.0 40.0 27.0 41.0 28-29 35.241286132834176 38.0 33.5 40.0 27.0 41.0 30-31 34.93616834737831 37.0 33.0 40.0 26.5 41.0 32-33 34.67768050695097 37.0 33.0 40.0 25.0 41.0 34-35 34.90836021485488 37.0 33.0 40.0 26.0 41.0 36-37 34.738115284734505 37.0 33.0 40.0 25.0 41.0 38-39 34.53865038731587 37.0 33.0 40.0 25.0 41.0 40-41 34.36761602829219 37.0 33.0 40.0 24.5 41.0 42-43 34.186670341296676 37.0 33.0 40.0 24.0 41.0 44-45 33.77688904343708 37.0 32.5 40.0 23.5 41.0 46-47 33.3476546489002 37.0 32.0 40.0 22.0 41.0 48-49 32.940598249495046 37.0 31.0 39.5 19.5 40.5 50-51 32.69336273037341 36.0 31.0 39.0 19.0 40.0 52-53 32.56292995020753 36.0 31.0 39.0 18.5 40.0 54-55 32.22170984973476 36.0 30.5 39.0 16.5 40.0 56-57 31.73616501801555 35.5 29.5 39.0 13.5 40.0 58-59 31.288177062910158 35.0 29.0 38.5 11.5 40.0 60-61 30.92351695385503 35.0 28.0 38.0 8.0 40.0 62-63 30.741385828752378 34.5 28.0 38.0 8.0 40.0 64-65 30.265349102181844 34.0 28.0 38.0 7.5 40.0 66-67 29.37438961682734 33.5 27.0 37.5 4.5 39.5 68-69 25.909895236051817 31.0 16.5 36.0 2.0 39.0 70-71 24.56908760663209 29.5 12.0 35.0 2.0 38.0 72-73 25.597195751733118 30.0 18.0 35.0 2.0 38.0 74-75 26.34553754411406 31.0 21.5 35.0 2.0 37.5 76-77 26.037185097772287 30.5 22.0 34.0 2.0 36.0 78-79 27.160590333010262 32.0 25.5 35.0 2.0 37.0 80-81 27.18179726400367 32.0 25.5 35.0 2.0 36.5 82-83 26.84928955097994 32.0 25.0 35.0 2.0 36.0 84-85 26.469671169938074 32.0 24.5 34.5 2.0 36.0 86-87 26.003517841685102 31.5 23.5 34.0 2.0 35.0 88-89 25.49312875015722 31.0 21.5 34.0 2.0 35.0 90-91 24.87784346076161 31.0 19.5 34.0 2.0 35.0 92-93 24.299737350271158 31.0 17.5 34.0 2.0 35.0 94-95 23.426558881630058 30.0 7.0 34.0 2.0 35.0 96-97 22.74050965145271 30.0 2.0 34.0 2.0 35.0 98-99 21.8717314905927 29.5 2.0 34.0 2.0 35.0 100-101 20.458879410480833 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.26928820825400024 1101 2 0.23178037425144637 1101 3 0.3545573147602603 1101 4 0.3510304558843913 1101 5 0.6094997413503904 1101 6 0.5055831061958997 1101 7 0.5581903594687958 1101 8 0.7004087239593417 1101 9 0.7097944808964485 1101 10-11 0.8440390745175179 1101 12-13 0.7607784934372646 1101 14-15 0.7406578928089047 1101 16-17 0.7431064359637887 1101 18-19 0.6563317544492406 1101 20-21 0.8469829007303247 1101 22-23 0.7932332186791413 1101 24-25 0.8911641422430705 1101 26-27 0.8329703146060226 1101 28-29 0.5913572522910471 1101 30-31 0.3027516154242349 1101 32-33 0.8121177591201558 1101 34-35 0.7602784697694531 1101 36-37 0.8254033408805981 1101 38-39 0.8605036091565808 1101 40-41 0.9153677256201789 1101 42-43 0.46297869006469483 1101 44-45 0.7307408558404873 1101 46-47 0.7562407800149842 1101 48-49 0.8088318135142885 1101 50-51 0.3214703923235547 1101 52-53 0.1368455961481061 1101 54-55 0.20317748128329782 1101 56-57 0.30662976047143786 1101 58-59 0.1700240482509514 1101 60-61 0.1851292603676491 1101 62-63 -0.23738732816235242 1101 64-65 -0.23243409591323783 1101 66-67 -0.2162135755647654 1101 68-69 -7.184734610249901 1101 70-71 -7.793685252136086 1101 72-73 -4.988465522084081 1101 74-75 -3.0850286071319033 1101 76-77 -0.4265661540381096 1101 78-79 -0.02866362262873423 1101 80-81 -0.2723328628910444 1101 82-83 -0.44238735683014596 1101 84-85 -0.4729735127917394 1101 86-87 -0.46492515593497075 1101 88-89 -0.6005948840298103 1101 90-91 -0.5139839410184841 1101 92-93 -0.3577935229263396 1101 94-95 -0.36752879355139 1101 96-97 -0.5478124919806859 1101 98-99 -0.590593738967204 1101 100-101 -0.4294106318812929 1102 1 0.4047813003730809 1102 2 0.37361878472029986 1102 3 0.43809661923352294 1102 4 0.3837174098968106 1102 5 0.5012318981890687 1102 6 0.4933843366850539 1102 7 0.6281398325381744 1102 8 0.6794845758386785 1102 9 0.6684545807711757 1102 10-11 0.7300713307718993 1102 12-13 0.6300272557943458 1102 14-15 0.7966335264327071 1102 16-17 0.7517820172319389 1102 18-19 0.5800502809237855 1102 20-21 0.7335142884790571 1102 22-23 0.6976943340616799 1102 24-25 0.47985094423147245 1102 26-27 0.7503343107489968 1102 28-29 0.8069483156790653 1102 30-31 0.604192400963079 1102 32-33 0.7341687589675985 1102 34-35 0.6309636696099759 1102 36-37 0.6673168368971858 1102 38-39 0.8251223347046945 1102 40-41 0.6661232923681482 1102 42-43 0.540187046879808 1102 44-45 0.632950089109876 1102 46-47 0.7695453558221743 1102 48-49 0.8610669614044895 1102 50-51 0.6309156530958049 1102 52-53 0.3024053861738807 1102 54-55 0.19196639414206373 1102 56-57 0.3916655645495375 1102 58-59 0.3973867083933591 1102 60-61 0.5739207946932297 1102 62-63 0.404571311829077 1102 64-65 0.26240629939553983 1102 66-67 0.1050654496791168 1102 68-69 -6.5932776337318195 1102 70-71 -7.410806283574843 1102 72-73 -1.3029176265692222 1102 74-75 0.28566333061531424 1102 76-77 0.7290720157400834 1102 78-79 0.42841733300436147 1102 80-81 0.19266534366770216 1102 82-83 0.09576993517988441 1102 84-85 -0.13011028777060574 1102 86-87 -0.21738536043675438 1102 88-89 -0.02745969709132723 1102 90-91 -0.08235474360529693 1102 92-93 0.05787236280992403 1102 94-95 0.3274193766805631 1102 96-97 -0.1770651244229242 1102 98-99 -0.08289078505838177 1102 100-101 -0.2885823810176511 1103 1 0.42147999095691446 1103 2 0.3151874958756302 1103 3 0.4525437266187602 1103 4 0.3480798951488566 1103 5 0.5189971889967708 1103 6 0.4809041476760214 1103 7 0.4912150025387447 1103 8 0.5425610063476896 1103 9 0.7122700641845654 1103 10-11 0.6001524066746811 1103 12-13 0.5122453998049181 1103 14-15 0.6104254950611718 1103 16-17 0.7207561776492852 1103 18-19 0.7915141744999019 1103 20-21 0.7879145763791868 1103 22-23 0.6797251034928422 1103 24-25 0.7702715632923827 1103 26-27 0.7637704903664044 1103 28-29 0.5865030368714059 1103 30-31 0.5967096027015728 1103 32-33 0.8644157303859856 1103 34-35 0.814829151090585 1103 36-37 1.0113940964005792 1103 38-39 1.1871853164289945 1103 40-41 1.1104174328526497 1103 42-43 0.8631710632126399 1103 44-45 1.0589621828479991 1103 46-47 0.9543492858353844 1103 48-49 0.9793007096691895 1103 50-51 0.8277742979988219 1103 52-53 0.7288247291800758 1103 54-55 0.6161627779954486 1103 56-57 0.8323693043100029 1103 58-59 0.8765613232660137 1103 60-61 0.8978014526954432 1103 62-63 0.6839645828988985 1103 64-65 0.6554768958513257 1103 66-67 0.5704698501981085 1103 68-69 -6.713294423704223 1103 70-71 -7.436775120865054 1103 72-73 -0.6694366106666472 1103 74-75 0.7606395070058234 1103 76-77 1.0451649900021849 1103 78-79 0.7688012502612267 1103 80-81 0.5211266893360893 1103 82-83 0.40150011200400826 1103 84-85 0.25554956948941765 1103 86-87 0.3395429296583359 1103 88-89 0.5102229925983863 1103 90-91 0.49361757058735733 1103 92-93 0.2900057586364646 1103 94-95 0.45200146561195353 1103 96-97 0.47504967260160313 1103 98-99 0.5129406820221831 1103 100-101 0.3540253462352112 1104 1 0.2078004432082423 1104 2 0.21688116303321436 1104 3 0.12170690445794818 1104 4 0.21120684985753257 1104 5 0.4215814379749432 1104 6 0.2829340454106273 1104 7 0.3541065648163837 1104 8 0.4452256155714309 1104 9 0.49357415480030653 1104 10-11 0.36481706554182125 1104 12-13 0.38074909616936026 1104 14-15 0.496065160547424 1104 16-17 0.5461674938611694 1104 18-19 0.5066602191624128 1104 20-21 0.5344965252133917 1104 22-23 0.4749380839995325 1104 24-25 0.3348233868597532 1104 26-27 0.5445808360499171 1104 28-29 0.5218449971235941 1104 30-31 0.4010786351518121 1104 32-33 0.4150525103499163 1104 34-35 0.24431220821357869 1104 36-37 0.3999814418855081 1104 38-39 0.5652896833929759 1104 40-41 0.6136854161724656 1104 42-43 0.492102664956775 1104 44-45 0.5053972182602919 1104 46-47 0.35858971086558 1104 48-49 0.5089206998232498 1104 50-51 0.4349403220593189 1104 52-53 0.3653967596776653 1104 54-55 0.31500112255370993 1104 56-57 0.3973254629868386 1104 58-59 0.4872976382368073 1104 60-61 0.5362836162373341 1104 62-63 0.26420744252392225 1104 64-65 0.1779012827553963 1104 66-67 0.24111622788505827 1104 68-69 -6.3786669146443025 1104 70-71 -4.360996288394961 1104 72-73 0.1332856055799816 1104 74-75 0.8305260304788753 1104 76-77 1.0038655120852198 1104 78-79 0.5006396279150813 1104 80-81 0.24253400718619744 1104 82-83 0.16624280408257874 1104 84-85 0.3128573357848623 1104 86-87 0.2898669045222313 1104 88-89 0.3238085281678771 1104 90-91 0.16926355526409864 1104 92-93 0.08753284531383443 1104 94-95 0.11152622318670424 1104 96-97 0.07868853345116378 1104 98-99 0.07024896543632053 1104 100-101 0.22338161619599006 1105 1 0.24836245885287767 1105 2 0.2149419257998595 1105 3 0.20886290774085126 1105 4 0.24337857766706605 1105 5 0.39404862709778143 1105 6 0.31465183192021584 1105 7 0.3215883890067346 1105 8 0.5122970768266342 1105 9 0.46687950113936694 1105 10-11 0.3674056204106151 1105 12-13 0.3380404829055621 1105 14-15 0.43678514776444644 1105 16-17 0.44790953899381947 1105 18-19 0.5228328766437187 1105 20-21 0.5427324730065095 1105 22-23 0.4590374281451517 1105 24-25 0.6205598249094777 1105 26-27 0.7426521302547187 1105 28-29 0.6150068253812577 1105 30-31 0.44494679607218046 1105 32-33 0.6095137001845359 1105 34-35 0.5392988804970145 1105 36-37 0.6603628978386737 1105 38-39 0.6540875015695065 1105 40-41 0.7038152796995618 1105 42-43 0.6170284001601019 1105 44-45 0.8214511246975391 1105 46-47 0.787116834766131 1105 48-49 0.8032954592343629 1105 50-51 0.49414552531607825 1105 52-53 0.3113534137308207 1105 54-55 0.412651159232162 1105 56-57 0.6360836974820181 1105 58-59 0.7710363714137429 1105 60-61 0.8505922091645672 1105 62-63 0.5734849132356317 1105 64-65 0.5747629141841095 1105 66-67 0.6498130168858793 1105 68-69 -3.0537230217889793 1105 70-71 -3.4518045218006854 1105 72-73 0.5684050466148207 1105 74-75 1.1179743719589403 1105 76-77 1.0372458132117224 1105 78-79 0.6742409439816974 1105 80-81 0.6218542573260102 1105 82-83 0.6318217063435689 1105 84-85 0.4911041055385752 1105 86-87 0.4930484883052664 1105 88-89 0.54309631242592 1105 90-91 0.6091675376003316 1105 92-93 0.6287561626442191 1105 94-95 0.6694106388371495 1105 96-97 0.5865308233489728 1105 98-99 0.5961911659984835 1105 100-101 0.7710069584677335 1106 1 0.12437229279906603 1106 2 0.14034720518778343 1106 3 0.15197925137792723 1106 4 0.14824129450664003 1106 5 0.3433281421825001 1106 6 0.32953457694760857 1106 7 0.27073840967073437 1106 8 0.40326998048998774 1106 9 0.376110270370134 1106 10-11 0.34905725710564184 1106 12-13 0.22927947534995496 1106 14-15 0.37671616178417366 1106 16-17 0.40868650551814056 1106 18-19 0.28216346890584987 1106 20-21 0.3459458844745029 1106 22-23 0.3086885773816448 1106 24-25 0.41485391076575695 1106 26-27 0.5063156990870468 1106 28-29 0.3879226752016365 1106 30-31 0.5204826295733014 1106 32-33 0.5504481370109673 1106 34-35 0.2850424137236729 1106 36-37 0.4858567675176957 1106 38-39 0.5738787421514928 1106 40-41 0.4521400118622836 1106 42-43 0.3464323231416486 1106 44-45 0.5419327549284461 1106 46-47 0.5267253843650437 1106 48-49 0.8071284728938011 1106 50-51 0.7103854184990723 1106 52-53 0.455597270163679 1106 54-55 0.3897068036841418 1106 56-57 0.5621524503299433 1106 58-59 0.7633584755391247 1106 60-61 0.9238495978703618 1106 62-63 0.623751425893655 1106 64-65 0.7231369220470292 1106 66-67 0.8592350606087891 1106 68-69 -2.8866533893962973 1106 70-71 -3.06848175006904 1106 72-73 1.1720267575942955 1106 74-75 1.6640191757568807 1106 76-77 1.7001123873319095 1106 78-79 1.2926452590494648 1106 80-81 0.8997876450568931 1106 82-83 0.6913102987540505 1106 84-85 0.7294023453893672 1106 86-87 0.8232837400352189 1106 88-89 0.7074145592706849 1106 90-91 0.8795069571007943 1106 92-93 0.7216143651938403 1106 94-95 1.0631442533422195 1106 96-97 1.0878635985776057 1106 98-99 1.1958966774590571 1106 100-101 1.1500784559191715 1107 1 -0.016504084795251117 1107 2 -0.033865690387617775 1107 3 -1.597641163222363E-4 1107 4 -0.26004073789808757 1107 5 0.23810966598025374 1107 6 0.11666238060845302 1107 7 0.12151233527362137 1107 8 0.023670234512479738 1107 9 0.17474585487476446 1107 10-11 0.08307424064475555 1107 12-13 0.11385210498853127 1107 14-15 0.09549629183020159 1107 16-17 -0.024240822693535335 1107 18-19 0.1010586396685369 1107 20-21 0.14761731874618533 1107 22-23 0.2131486952885382 1107 24-25 0.0737196891030365 1107 26-27 0.3483396176649691 1107 28-29 0.2829262438804392 1107 30-31 0.17114837081880552 1107 32-33 0.18153239717046432 1107 34-35 0.032938410621447645 1107 36-37 0.11470473056079555 1107 38-39 0.294653004952373 1107 40-41 0.20415892866991925 1107 42-43 -0.09982961226244669 1107 44-45 0.22129769870068827 1107 46-47 0.47181527573108895 1107 48-49 0.497803768082548 1107 50-51 0.4578816671713568 1107 52-53 0.12420632842587764 1107 54-55 0.35648347183073525 1107 56-57 0.7028686588756692 1107 58-59 0.7800438192281867 1107 60-61 0.6047527827054466 1107 62-63 0.4792129094524462 1107 64-65 0.2291884459009772 1107 66-67 0.46211231491789917 1107 68-69 -2.6547410271557297 1107 70-71 -2.8580531714147988 1107 72-73 1.1738424564090444 1107 74-75 1.418733147555887 1107 76-77 1.2297772125228512 1107 78-79 1.085393197926706 1107 80-81 0.8054515036135719 1107 82-83 0.690341641307878 1107 84-85 0.5802897943179204 1107 86-87 0.6537677877599712 1107 88-89 0.4979082733380231 1107 90-91 0.5492112906570519 1107 92-93 0.5073362780056776 1107 94-95 0.764091442515916 1107 96-97 0.7495797115838911 1107 98-99 0.8544695993358999 1107 100-101 1.2471479805261971 1108 1 -0.08759019143801083 1108 2 -0.047885320483267435 1108 3 0.06346051601492775 1108 4 0.09555213939945162 1108 5 -0.09746721000629321 1108 6 -0.1290496871489495 1108 7 0.06716450406814545 1108 8 0.2931026682939901 1108 9 0.30719543520530124 1108 10-11 0.23978967018437203 1108 12-13 0.04105438077240109 1108 14-15 0.27056625636172527 1108 16-17 0.28926579303389843 1108 18-19 0.34801872021192537 1108 20-21 0.2977724697744506 1108 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34-35 0.49115911176900795 2312 36-37 0.7287569221150534 2312 38-39 0.6481440985672862 2312 40-41 0.32521512168764133 2312 42-43 0.09944957375774521 2312 44-45 0.6924559368788223 2312 46-47 0.7269286569309017 2312 48-49 0.8393868188551323 2312 50-51 0.8871104035750719 2312 52-53 0.811443193232364 2312 54-55 0.762629612151791 2312 56-57 0.7893732184186 2312 58-59 0.9279888960133214 2312 60-61 1.1011667979744502 2312 62-63 1.2239374019234113 2312 64-65 1.1738076602876824 2312 66-67 1.4940096699060597 2312 68-69 4.1276854390312465 2312 70-71 4.943620314800622 2312 72-73 3.1062201726652425 2312 74-75 2.485172533274749 2312 76-77 2.3806524066183137 2312 78-79 1.6255265864438115 2312 80-81 1.3742244361667808 2312 82-83 1.4813153499548584 2312 84-85 1.4078425958064749 2312 86-87 1.272980974120106 2312 88-89 1.4404783421608904 2312 90-91 1.6508611381174632 2312 92-93 1.9509388818857651 2312 94-95 2.237517368708584 2312 96-97 2.2418852728210723 2312 98-99 2.464017783825099 2312 100-101 2.402893046236173 2313 1 -0.21829932605339408 2313 2 -0.2007775671255132 2313 3 -0.11207271811205644 2313 4 0.04440363512595269 2313 5 0.010200296098716422 2313 6 0.029613698076936146 2313 7 -2.556519103990464 2313 8 -3.2256368387267678 2313 9 -1.0182300074076451 2313 10-11 -0.09558740933334064 2313 12-13 -0.042529353201963715 2313 14-15 -0.5364750096639099 2313 16-17 -0.19374035012849333 2313 18-19 0.26330460620093277 2313 20-21 -2.5545221540045446 2313 22-23 -0.6295689078187223 2313 24-25 0.026283071740259345 2313 26-27 -1.4546394466324486 2313 28-29 -1.7425951697437227 2313 30-31 -0.7493537206088448 2313 32-33 -0.8254065419507839 2313 34-35 -1.1577197105932626 2313 36-37 -1.9698308463256566 2313 38-39 -1.8537743771563413 2313 40-41 -0.9697322843581091 2313 42-43 0.05100441444402293 2313 44-45 0.015239477378358401 2313 46-47 -0.022810561673203722 2313 48-49 -2.1927130002391664 2313 50-51 -0.2917108645155011 2313 52-53 -0.2002117291532528 2313 54-55 -0.20640487664372387 2313 56-57 -0.1429403320015652 2313 58-59 -0.13625815697453447 2313 60-61 0.022700776149051194 2313 62-63 0.3094299322688876 2313 64-65 0.3391467125752641 2313 66-67 0.9945239381133426 2313 68-69 1.6504700737184557 2313 70-71 4.158460417508373 2313 72-73 3.3197417792245325 2313 74-75 1.9266663182887562 2313 76-77 1.8126759973045878 2313 78-79 1.1062287631478256 2313 80-81 1.2909129865869495 2313 82-83 1.2468564622331044 2313 84-85 0.9128667408811708 2313 86-87 0.9748275981863976 2313 88-89 0.6188619351814317 2313 90-91 1.1454959712173682 2313 92-93 1.3807175641920217 2313 94-95 1.6482334780689953 2313 96-97 1.677009061920046 2313 98-99 2.008018717531918 2313 100-101 2.0097495909373855 2314 1 -0.31301838671765125 2314 2 -0.2468719839553586 2314 3 -0.2008260051909332 2314 4 -0.055841287553171526 2314 5 -0.174425667274285 2314 6 -2.4409998952102043 2314 7 -0.7403640919404566 2314 8 -0.04660342035301568 2314 9 -0.1705774838097156 2314 10-11 -0.24501858386388875 2314 12-13 -2.46855448641103 2314 14-15 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-2.32070291310162 2315 100-101 -3.9605695056125754 2316 1 -0.5456853057831239 2316 2 -0.46257981054540664 2316 3 -1.0425420201346647 2316 4 -0.36273788139056506 2316 5 -0.14830885643617364 2316 6 -0.4926590342141779 2316 7 -0.58105374892952 2316 8 -1.010260386907298 2316 9 -5.364313682994997 2316 10-11 -1.5461265284156553 2316 12-13 -0.7225635924043914 2316 14-15 -0.5968355770327562 2316 16-17 -0.4379448027428694 2316 18-19 -0.5007233233830632 2316 20-21 -3.8982911297817964 2316 22-23 -1.2965298715398035 2316 24-25 -0.38037922014721914 2316 26-27 -0.6521558969062511 2316 28-29 0.11862324076237485 2316 30-31 -0.1512696530854427 2316 32-33 -1.246691567785433 2316 34-35 -0.44659797304689874 2316 36-37 -0.27926160060935246 2316 38-39 -5.576563802611204 2316 40-41 -5.076569015390618 2316 42-43 -3.624009179112768 2316 44-45 -2.534289716337419 2316 46-47 -2.655096225093981 2316 48-49 -3.0213520712955173 2316 50-51 -1.382857042862966 2316 52-53 -1.1587034239730798 2316 54-55 -0.20099367500090537 2316 56-57 -3.542004377664874 2316 58-59 -0.7121743765028903 2316 60-61 -0.5706983444789309 2316 62-63 -0.23397008539583908 2316 64-65 0.10111732289146147 2316 66-67 -0.20739972267980278 2316 68-69 2.653337141153539 2316 70-71 3.567746541868029 2316 72-73 2.3584913514070074 2316 74-75 0.5774644049783859 2316 76-77 -1.9840799728199485 2316 78-79 -0.26626436417066657 2316 80-81 -0.09999768511416463 2316 82-83 0.07712123987839803 2316 84-85 0.38737675181121745 2316 86-87 0.32420236357679144 2316 88-89 0.11950400648067117 2316 90-91 0.15067187322231845 2316 92-93 0.01687877135506355 2316 94-95 0.1869016269163737 2316 96-97 0.5276941909829311 2316 98-99 0.32993622327295213 2316 100-101 0.3459558569460448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 29.0 4 103.0 5 272.0 6 535.0 7 1406.0 8 3111.0 9 5144.0 10 8266.0 11 12005.0 12 15339.0 13 19571.0 14 23802.0 15 30143.0 16 34929.0 17 39180.0 18 36204.0 19 32134.0 20 31968.0 21 32532.0 22 34209.0 23 38324.0 24 45020.0 25 55360.0 26 69440.0 27 84544.0 28 103163.0 29 122180.0 30 143438.0 31 164230.0 32 189135.0 33 217717.0 34 248149.0 35 275350.0 36 274997.0 37 219677.0 38 87070.0 39 4535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.60668041090639 21.057731726845816 16.17503629692886 22.160551565318933 2 15.716041082920263 21.762485251018507 39.23301226279069 23.288461403270546 3 19.026430818494173 25.8909784226741 30.642888005658463 24.43970275317327 4 13.071829120105683 18.285647430633357 37.15316678872303 31.489356660537933 5 13.014109099518354 38.60048386738778 34.14661033878116 14.238796694312708 6 30.55681742514483 37.58943038302469 17.363625602059766 14.49012658977072 7 27.25775186629279 33.97022809834198 20.692729411590623 18.07929062377461 8 25.963369611056443 35.57168857880602 19.509880808813193 18.955061001324346 9 26.03835425899483 17.010713149503186 20.64822692936572 36.30270566213627 10-11 24.382495324526857 27.639195911111443 27.089811630690342 20.88849713367136 12-13 25.535823943297252 25.185593477408425 27.83319522643366 21.445387352860664 14-15 22.65736787978781 26.258980031222023 26.785370780032704 24.298281308957463 16-17 21.090785197318954 29.443868275352962 27.526344245032597 21.93900228229548 18-19 20.88965431392246 27.73257137741548 30.581323707277086 20.79645060138498 20-21 22.18384001302151 26.647683133448258 30.53053395580086 20.637942897729374 22-23 21.55148591584465 26.747558000381026 30.334990002644997 21.365966081129322 24-25 21.229678248021376 27.01785850370939 30.70893901828172 21.04352422998752 26-27 21.38863352601156 27.034376878612715 30.514811560693644 21.06217803468208 28-29 21.07149330975625 27.058419866898 30.730303826220133 21.139782997125618 30-31 21.290718405543323 27.0255368097734 30.654992175059665 21.028752609623613 32-33 21.260218422125433 26.9778366872521 30.33732908257824 21.424615808044226 34-35 21.44990506419843 27.024768394562994 30.40640661748317 21.118919923755406 36-37 21.608846122943618 26.81752696146585 30.494087120144815 21.07953979544572 38-39 21.437691209916736 27.00937507174422 30.445747298899562 21.10718641943948 40-41 21.466234923098455 26.97911059146549 30.447399579264754 21.107254906171303 42-43 21.322115798474613 27.163022714175195 30.169049800426933 21.34581168692326 44-45 21.468380703717337 27.32604592371323 29.914243844861403 21.29132952770803 46-47 21.55145667157358 27.466593260450388 29.29243096508194 21.689519102894092 48-49 21.65591548325903 27.48945058639116 29.244348039204798 21.610285891145008 50-51 21.887338700272664 27.69125968816466 28.99944652281196 21.421955088750718 52-53 21.76933989371005 27.743545657205203 28.97740112471533 21.509713324369418 54-55 21.648132260378368 27.730694324052436 29.00731432344544 21.61385909212376 56-57 21.741763332825148 27.914675247592164 28.64305728034197 21.70050413924071 58-59 21.717679545643335 27.692997332787694 28.85604768236326 21.733275439205705 60-61 21.728052809187886 27.66462821090081 28.997566654321734 21.609752325589568 62-63 21.801123017531125 27.524787161412917 29.003942972742863 21.670146848313095 64-65 21.762168107421292 27.49996809147628 28.974456348122178 21.763407452980246 66-67 21.419698685619252 27.69105132859963 29.030146073799994 21.85910391198112 68-69 21.750916687381185 27.284786697588753 29.358723185566443 21.60557342946361 70-71 21.389581313222283 27.123172100862607 29.704092422238755 21.783154163676357 72-73 21.638852264664244 27.52487776007521 28.76350264307056 22.072767332189986 74-75 21.58453466510793 27.753202219961555 28.47441768018866 22.187845434741853 76-77 21.03755376221523 27.88809156043961 28.939909013369984 22.134445663975175 78-79 21.519944843232576 27.7377160295933 28.52366666709831 22.218672460075815 80-81 21.33823184496218 27.98762473484481 28.42511691984193 22.249026500351075 82-83 21.49406327085365 28.128953688481534 28.167722956585816 22.209260084079 84-85 21.528986214938058 27.8966608661683 28.113791128788822 22.460561790104823 86-87 21.449311605287562 27.858871777721962 28.22505255860428 22.4667640583862 88-89 21.63952825723339 27.661312354328306 28.207884661590356 22.491274726847944 90-91 21.43135394296559 27.83138279867042 28.30298860014687 22.434274658217124 92-93 21.36722635884152 27.92678609282034 28.154081269123925 22.55190627921422 94-95 21.226919434508098 27.82566393763684 28.23368561191404 22.71373101594102 96-97 21.234300216824373 27.637706050691534 28.12087024325112 23.00712348923298 98-99 21.22299696171884 27.55162710093516 28.031991487485037 23.19338444986096 100-101 21.24107753649918 27.51411555646981 28.151715824925294 23.09309108210572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4373.0 1 2944.5 2 1464.0 3 1425.0 4 1273.0 5 1216.0 6 1856.0 7 3457.0 8 5188.0 9 6009.5 10 5494.5 11 4479.0 12 4101.5 13 4000.5 14 4049.5 15 4263.0 16 4474.0 17 4862.0 18 5311.5 19 5828.5 20 6536.0 21 7475.0 22 8830.5 23 10382.5 24 12323.0 25 15165.5 26 19093.5 27 23114.0 28 26574.5 29 30757.5 30 35767.5 31 41347.0 32 47825.5 33 54322.0 34 60714.5 35 66516.0 36 73443.5 37 80207.5 38 82937.5 39 83863.0 40 84856.5 41 85863.5 42 86985.0 43 88736.5 44 91416.0 45 94662.0 46 97639.0 47 99895.0 48 104901.0 49 103112.0 50 93639.0 51 88255.0 52 85383.5 53 82526.5 54 79230.5 55 73847.0 56 66689.0 57 59774.0 58 53516.5 59 47157.0 60 40046.5 61 32925.0 62 25852.0 63 19507.0 64 14236.0 65 10400.0 66 7902.0 67 5993.0 68 4511.5 69 3469.0 70 2638.5 71 1878.0 72 1304.0 73 1037.5 74 809.0 75 542.0 76 358.5 77 239.0 78 155.5 79 94.0 80 56.5 81 38.5 82 26.0 83 16.0 84 9.5 85 6.0 86 3.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018940374812260935 2 0.018163523501601792 3 4.4391503466236556E-4 4 0.08441784242495987 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010542982073231183 12-13 0.0 14-15 0.0 16-17 7.583548508815413E-4 18-19 5.5489379332795694E-5 20-21 0.0 22-23 1.849645977759857E-4 24-25 0.003033419403526165 26-27 0.003514327357743728 28-29 0.001109787586655914 30-31 1.6646813799838712E-4 32-33 5.179008737727599E-4 34-35 0.002866951265527778 36-37 0.08955985824313226 38-39 0.09817920849949319 40-41 0.0826421822863104 42-43 0.08617500610383173 44-45 0.0853056724942846 46-47 0.0838999415511871 48-49 0.07849897529612831 50-51 0.07824002485924193 52-53 0.060945834967187286 54-55 0.051716101538165596 56-57 0.02929839228771613 58-59 0.021659354399567923 60-61 0.013206472281205377 62-63 0.002090099954868638 64-65 0.006677221979713083 66-67 0.16071573900755395 68-69 0.7367879787808613 70-71 0.9036445424345779 72-73 0.3681165424937667 74-75 0.05406515192992061 76-77 0.20201833369093156 78-79 0.014001820051642114 80-81 0.003181391081746954 82-83 0.0017756601386494622 84-85 1.4797167822078855E-4 86-87 0.0017386672190942655 88-89 3.514327357743728E-4 90-91 0.00425418574884767 92-93 0.0046981007835100366 94-95 0.07909086200901148 96-97 0.03821368590051864 98-99 0.013909337752754124 100-101 0.012984514763874194 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2703220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.94734286080394 #Duplication Level Percentage of deduplicated Percentage of total 1 71.24391325383279 36.29688075288392 2 14.648215995186836 14.92575365211792 3 5.7731706605292965 8.8238311510776 4 2.8504745094127597 5.8089640858813505 5 1.600581185135825 4.077267920783338 6 0.9906458094205614 3.0282463023700785 7 0.6211421510127728 2.21518794930615 8 0.41905592306333606 1.707982863212677 9 0.31542090621501206 1.446287134896153 >10 1.418830186839068 12.377343116309893 >50 0.07752363187788192 2.711943238806854 >100 0.038091836215458814 3.519769833539039 >500 0.0016870219736366042 0.5311665930673584 >1k 9.535341589787226E-4 0.9066154997909205 >5k 1.4669756291980348E-4 0.5627436136742703 >10k+ 1.4669756291980348E-4 1.060016292282645 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15364 0.5683592160460488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13002 0.48098194005667316 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9774 0.3615687957324968 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5285 0.19550757984921688 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3466 0.1282174591783133 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3074 0.113716234712676 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2768 0.10239640132878568 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2758 0.10202647213323371 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.699291955519713E-5 0.0 0.0 0.0 0.0 7 7.398583911039426E-5 0.0 0.0 0.0 0.0 8 7.398583911039426E-5 0.0 0.0 0.0 0.0 9 7.398583911039426E-5 0.0 0.0 0.0 0.0 10-11 7.398583911039426E-5 0.0 0.0 0.0 0.0 12-13 7.398583911039426E-5 0.0 0.0 0.0 0.0 14-15 1.109787586655914E-4 0.0 0.0 0.0 0.0 16-17 1.109787586655914E-4 0.0 0.0 0.0 0.0 18-19 1.109787586655914E-4 0.0 0.0 1.109787586655914E-4 0.0 20-21 1.109787586655914E-4 0.0 0.0 2.2195751733118278E-4 0.0 22-23 1.4797167822078853E-4 0.0 0.0 5.54893793327957E-4 0.0 24-25 2.5895043688637997E-4 0.0 0.0 8.878300693247313E-4 0.0 26-27 2.5895043688637997E-4 0.0 0.0 0.0012947521844318996 0.0 28-29 2.5895043688637997E-4 0.0 0.0 0.002774468966639785 0.0 30-31 2.5895043688637997E-4 0.0 0.0 0.006529250301492295 0.0 32-33 2.5895043688637997E-4 0.0 0.0 0.014186784649418102 0.0 34-35 2.5895043688637997E-4 0.0 0.0 0.027837171965285845 0.0 36-37 2.5895043688637997E-4 0.0 0.0 0.04938554760618817 0.0 38-39 2.9594335644157706E-4 0.0 0.0 0.08007117437722419 0.0 40-41 2.9594335644157706E-4 0.0 0.0 0.11530693025354948 0.0 42-43 3.144398162191756E-4 0.0 0.0 0.14965485606055 0.0 44-45 3.329362759967742E-4 3.699291955519713E-5 0.0 0.1936949267910122 0.0 46-47 3.6992919555197133E-4 3.699291955519713E-5 0.0 0.2481485043762624 0.0 48-49 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.3057464801237043 0.0 50-51 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.3665073504931156 0.0 52-53 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.42896989516206596 0.0 54-55 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.501124584754478 0.0 56-57 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.5821760714999149 0.0 58-59 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.6664829351662092 0.0 60-61 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.7537492323969193 0.0 62-63 4.0692211510716847E-4 3.699291955519713E-5 0.0 0.8410710190069621 3.699291955519713E-5 64-65 4.0692211510716847E-4 9.248229888799283E-5 0.0 0.932628494906075 7.398583911039426E-5 66-67 4.0692211510716847E-4 1.2947521844318996E-4 0.0 1.0319359874520018 7.398583911039426E-5 68-69 4.439150346623656E-4 1.4797167822078853E-4 0.0 1.1468729885099993 7.398583911039426E-5 70-71 4.8090795421756275E-4 1.4797167822078853E-4 0.0 1.2918112473272614 7.398583911039426E-5 72-73 4.8090795421756275E-4 1.4797167822078853E-4 0.0 1.4532668447259196 7.398583911039426E-5 74-75 5.179008737727599E-4 1.4797167822078853E-4 0.0 1.625246927738031 7.398583911039426E-5 76-77 5.54893793327957E-4 1.4797167822078853E-4 0.0 1.8242133455656586 7.398583911039426E-5 78-79 5.54893793327957E-4 1.4797167822078853E-4 0.0 2.0518307795887867 7.398583911039426E-5 80-81 5.54893793327957E-4 1.4797167822078853E-4 0.0 2.3053062643809974 7.398583911039426E-5 82-83 5.918867128831541E-4 1.4797167822078853E-4 0.0 2.570268050695097 7.398583911039426E-5 84-85 5.918867128831541E-4 1.4797167822078853E-4 0.0 2.8396319944362647 7.398583911039426E-5 86-87 5.918867128831541E-4 1.4797167822078853E-4 0.0 3.1260866670119336 7.398583911039426E-5 88-89 5.918867128831541E-4 1.4797167822078853E-4 0.0 3.440397008012666 7.398583911039426E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11685 0.0 34.779083 1 GTATCAA 17060 0.0 29.581985 1 GTACATG 15005 0.0 24.489395 1 TACATGG 14725 0.0 24.206335 2 TATCAAC 21510 0.0 23.455097 2 ATCAACG 21805 0.0 22.9803 3 TCAACGC 22070 0.0 22.708996 4 CAACGCA 22310 0.0 22.37726 5 ACATGGG 15310 0.0 22.222446 3 AACGCAG 22990 0.0 21.75663 6 GAGTACT 12830 0.0 20.967033 12-13 AGTACTT 13340 0.0 20.627386 12-13 TCGACGA 60 0.004546644 19.953323 70-71 CATGGGA 10035 0.0 19.816776 4 GTACTTT 13720 0.0 19.572378 14-15 ACGCAGA 25795 0.0 19.262112 7 CGCAGAG 25885 0.0 19.121876 8 CATGGGG 5015 0.0 18.17049 4 GCAGAGT 26595 0.0 17.862648 9 TACTTTT 15495 0.0 17.63622 14-15 >>END_MODULE