FastQCFastQC Report
Fri 27 May 2016
SRR939370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625317
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA195203.121616716001644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181312.899489378986978No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA161122.5766131418144718No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131172.0976560688418835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91581.464537186738886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80851.2929442186922793No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG80191.282389572009077No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT80091.2807903831176828No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72641.1616508107088084No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA72211.1547742984758131No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71831.1486973806885148No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT65861.053225803872276No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC54770.8758757558166498No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG51540.8242219546246145No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.7823232056700842No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43350.6932483844194225No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42950.6868516288538453No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.6854123588515905No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG42340.6770965766163403No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT33930.5426047908500808No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33690.5387667375107346No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33190.5307707930537632No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT31440.5027849874543631No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG31170.49846717744759855No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA31010.4959084752213678No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG28050.4485724840360969No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27640.44201580958138037No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26890.43002189289592324No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.4140300039819803No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23350.3734106061405655No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA21860.3495826916587907No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC20700.3310321005186169No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18790.30048759269298614No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT17890.28609489267043753No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17610.28161716377453355No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC15590.24931354816836898No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA14990.2397184148200033No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT14690.23492084814582043No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT13970.22340668812778158No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13680.21876904034273817No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA13570.21700993256220444No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC13570.21700993256220444No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13320.21301196033371872No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT13250.21189252810974274No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.2037366647636319No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC12230.19558080141752104No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12090.19334193696956903No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11730.1875848569605496No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT11680.18678526251485247No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT10690.17095329249004906No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG10620.16983386026607306No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC10490.16775491470726048No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10260.16407678025705363No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC9660.15448164690868793No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9570.15304237690643305No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9520.15224278246073591No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC9330.14920432356708677No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.14888448578880792No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG8990.14376708133634622No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7750.12393713908305708No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT7610.12169827463510507No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA7590.12137843685682621No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA7300.11674078907178279No Hit
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC6760.10810516905825364No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6740.1077853312799748No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6580.10522662905374394No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6530.10442703460804681No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6490.10378735905148909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAATC300.00960412148.3756148
GTACCGT150.009898677548.00999512-13
GATGGAA458.7906973E-443.0040669
GGGATTC1301.6916601E-1037.208967
TACCTGG1451.2732926E-1136.9498752
TGCATGG550.00228011735.423022
ACCTGGG1502.1827873E-1135.2245063
ATATGGG701.9219109E-434.309583
TACAGGG1158.302959E-833.882892
TCTTCGG600.00361018832.2530489
CATTCCG600.00361018832.2530489
GGATGGA600.00361163632.250418
TCACGCA600.003890660831.7640134
AAAAAGT3250.030.736091
ATGGAGT953.0809475E-530.5505166
GGAGATA650.005343022329.769618
TATGGGA650.005755054829.320634
TCTATAG1153.5391968E-629.2377323
GCTTATG2153.092282E-1127.8769191
GGATGAC700.007673328327.6432088
Produced by FastQC (version 0.11.5)