FastQCFastQC Report
Fri 27 May 2016
SRR939369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106650
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129021.1658609316405368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93960.8490489314598112No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86770.7840780734649617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70560.6375999638548773No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.23340712962544616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.21741291284507297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22850.20647901323815118No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.18659919577102066No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18470.16690010391722768No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.14295396015000225No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15730.1421406948899833No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.10491121854244792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82100.034.3496321
GTATCAA125500.033.7855571
GTACATG93800.027.6302361
GTATCGA906.908467E-427.5831851
TATCAAC154650.027.3645232
ATCAACG158650.026.7320043
TACATGG95000.026.1346652
TCAACGC160700.026.132584
CAACGCA162100.025.8395045
AACGCAG163750.025.3080226
ACATGGG98900.023.8824353
GAGTACT89600.023.71383512-13
ACGCAGA179600.022.9775147
CGCAGAG180000.022.8932178
AGTACTT92450.022.25811412-13
GCAGAGT177900.022.1761889
CATGGGG23850.022.0350274
GTACTTT99000.021.85513314-15
CATGGGA73000.021.5973434
ACTTTTT114450.020.10096416-17
Produced by FastQC (version 0.11.5)