FastQCFastQC Report
Fri 27 May 2016
SRR939369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106650
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171871.5530655582162383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148891.345411828491393No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122501.1069443816924953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118521.0709799846383228No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43900.3966927212759228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.39289748339583425No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34600.31265531107396194No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27250.24623864817241223No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA23420.21162969321827135No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23200.2096417114715583No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22390.20232232413138754No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17360.15686983237699362No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12240.11060407536258075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA352.824108E-454.144057
GGTATCA106300.031.1686941
GTATCAA149600.026.8682251
GGGACCG1201.7413362E-423.6880235
GTACATG98750.023.5198041
TACATGG99200.023.3091162
GAGTACT115950.021.53368212-13
ACATGGG102400.021.5135353
TATCAAC186550.021.513242
AGTACTT116750.021.22380412-13
ATCAACG189150.021.1645553
TCAACGC191350.020.9212234
CAACGCA194300.020.5791995
GTACTTT122350.020.36855314-15
AACGCAG196850.020.2885466
CATGGGA75050.019.758434
CATGGGG25350.019.6232134
ACTTTTT134750.019.07433516-17
TACTTTT130350.018.97308314-15
CATATAG3558.185452E-1118.683513
Produced by FastQC (version 0.11.5)