##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939369_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1106650 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.730324854289975 31.0 30.0 31.0 28.0 34.0 2 30.96669226946189 31.0 31.0 33.0 28.0 34.0 3 30.895233361948222 31.0 30.0 34.0 27.0 34.0 4 34.87684181990693 35.0 35.0 37.0 32.0 37.0 5 34.51898341842497 35.0 35.0 37.0 30.0 37.0 6 34.523899155107756 35.0 35.0 37.0 30.0 37.0 7 34.37786382325035 35.0 35.0 37.0 30.0 37.0 8 34.41328694709258 35.0 35.0 37.0 30.0 37.0 9 35.65761442190394 37.0 35.0 39.0 30.0 39.0 10-11 35.532525640446394 37.0 35.0 39.0 30.0 39.0 12-13 35.47100483441015 37.0 34.5 39.0 30.0 39.0 14-15 36.124145393755924 38.0 34.0 40.0 29.0 41.0 16-17 36.161833461347314 38.0 34.0 40.0 29.5 41.0 18-19 36.231258753896896 38.0 34.0 40.0 30.0 41.0 20-21 36.192776397234894 38.0 34.0 40.0 29.0 41.0 22-23 36.00501332851398 38.0 34.0 40.0 29.0 41.0 24-25 35.76261329236886 38.0 34.0 40.0 28.0 41.0 26-27 35.28644783806985 38.0 33.5 40.0 27.0 41.0 28-29 34.94992725794063 37.0 33.0 40.0 26.5 41.0 30-31 34.60886865766051 37.0 33.0 39.0 26.0 40.0 32-33 34.20487642886188 36.0 33.0 39.0 25.0 40.0 34-35 33.98256178556906 36.0 33.0 39.0 24.5 40.0 36-37 33.79823792526996 36.0 33.0 39.0 24.0 41.0 38-39 33.671357701170194 36.0 33.0 39.0 23.5 41.0 40-41 33.370034337866535 36.0 32.0 39.0 22.0 41.0 42-43 32.95458184611214 36.0 31.0 39.0 20.0 40.0 44-45 32.59731983915421 36.0 31.0 39.0 19.0 40.0 46-47 32.06756381873221 35.0 30.5 39.0 15.5 40.0 48-49 31.58928297112908 35.0 30.0 39.0 10.5 40.0 50-51 31.260083133782135 35.0 30.0 39.0 8.5 40.0 52-53 30.873208331450776 35.0 29.0 39.0 7.5 40.0 54-55 30.626255365291648 35.0 28.0 38.5 6.5 40.0 56-57 30.21596394524014 35.0 28.0 38.0 2.0 40.0 58-59 29.781445353092668 34.0 27.0 38.0 2.0 40.0 60-61 29.465623277458995 34.0 27.0 38.0 2.0 40.0 62-63 29.071928794108345 34.0 26.0 38.0 2.0 40.0 64-65 28.61175936384584 33.5 26.0 37.0 2.0 39.5 66-67 26.840331631500476 32.0 20.5 36.0 2.0 39.0 68-69 23.154271901685266 27.0 10.5 34.0 2.0 38.0 70-71 22.52899200289161 27.0 10.0 34.0 2.0 37.0 72-73 24.352849139294264 29.0 15.5 34.0 2.0 37.0 74-75 24.96226765463335 30.0 18.0 34.0 2.0 37.0 76-77 25.187215470112502 30.5 20.5 34.0 2.0 36.0 78-79 26.018815795418604 31.5 22.0 35.0 2.0 36.0 80-81 25.872586635340895 32.0 21.5 35.0 2.0 36.0 82-83 25.44818415939999 31.0 20.0 34.5 2.0 36.0 84-85 25.07654271901685 31.0 19.5 34.0 2.0 35.0 86-87 24.592239642163285 31.0 18.0 34.0 2.0 35.0 88-89 24.03690326661546 31.0 13.0 34.0 2.0 35.0 90-91 23.4957140920797 30.0 7.0 34.0 2.0 35.0 92-93 22.853953372791757 30.0 2.0 34.0 2.0 35.0 94-95 21.402891609813402 29.0 2.0 34.0 2.0 35.0 96-97 20.53719197578277 28.0 2.0 33.0 2.0 35.0 98-99 20.137422852753808 28.5 2.0 33.0 2.0 35.0 100-101 18.980072290245335 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3285904919649312 1101 2 0.27058959293643525 1101 3 0.2356820524822183 1101 4 0.23883587908893134 1101 5 0.3277080708895852 1101 6 0.4645993178881085 1101 7 0.48725607315058994 1101 8 0.37419446145691637 1101 9 0.606640570108361 1101 10-11 0.7833207207063921 1101 12-13 0.6595582842612444 1101 14-15 0.4876815942439663 1101 16-17 0.6495444681864839 1101 18-19 0.7716870845538466 1101 20-21 0.7364475329812095 1101 22-23 0.7068662577353777 1101 24-25 0.8012183566799251 1101 26-27 0.8512969570586293 1101 28-29 0.5692467690542884 1101 30-31 0.7312414838763033 1101 32-33 0.6824617644453284 1101 34-35 0.7185866373696612 1101 36-37 1.0383102168684388 1101 38-39 0.9271624076974092 1101 40-41 0.9536665417664736 1101 42-43 1.0291149714494878 1101 44-45 0.28822527092894745 1101 46-47 0.4683823235258693 1101 48-49 0.5077577250766332 1101 50-51 0.5650695927929803 1101 52-53 0.599885017402034 1101 54-55 0.2830954252794555 1101 56-57 0.2845673049915476 1101 58-59 0.276183157561551 1101 60-61 0.17389638623402348 1101 62-63 0.14077835421102947 1101 64-65 -0.5029929388280934 1101 66-67 -3.3775911852961116 1101 68-69 -7.532313803987034 1101 70-71 -10.527186250135156 1101 72-73 -10.534104179206235 1101 74-75 -9.158449841878038 1101 76-77 -4.583288914001415 1101 78-79 -1.9872853409350313 1101 80-81 -1.4684191090092291 1101 82-83 -1.195934331585633 1101 84-85 -1.2390998057581335 1101 86-87 -1.3953131548947937 1101 88-89 -1.1472112322324968 1101 90-91 -1.2755476913812807 1101 92-93 -1.831751537878855 1101 94-95 -5.172621778895923 1101 96-97 -2.1974583863912933 1101 98-99 -1.3306769512047119 1101 100-101 -1.075377397325795 1102 1 0.24286010112897927 1102 2 0.23550619590276511 1102 3 0.3013520608961606 1102 4 0.23380016107154233 1102 5 0.5082790092368228 1102 6 0.48091961969022634 1102 7 0.318075855267935 1102 8 0.48972616222847165 1102 9 0.4021872290026707 1102 10-11 0.5753640442692856 1102 12-13 0.39028650286980593 1102 14-15 0.5641976164831775 1102 16-17 0.6840196415522328 1102 18-19 0.46721999517356494 1102 20-21 0.7563509101073862 1102 22-23 0.7974346664000933 1102 24-25 0.6596872938283127 1102 26-27 0.9154634541632447 1102 28-29 0.5461185966399213 1102 30-31 0.19023258049367797 1102 32-33 0.14165468573370532 1102 34-35 0.601018267200125 1102 36-37 0.6168711574703991 1102 38-39 0.5812533442848604 1102 40-41 0.8118528143892547 1102 42-43 0.7840934966445801 1102 44-45 0.7847002133236813 1102 46-47 0.7345156487935505 1102 48-49 0.5210965760508373 1102 50-51 0.36064402320196365 1102 52-53 0.5652786854966863 1102 54-55 0.42507578174472016 1102 56-57 0.43265794461627394 1102 58-59 0.3334425215113761 1102 60-61 0.054724417316119656 1102 62-63 0.06317236876220278 1102 64-65 0.14032897427181368 1102 66-67 -3.1725903878961823 1102 68-69 -7.847144617719909 1102 70-71 -10.529355452900209 1102 72-73 -9.081509054894337 1102 74-75 -7.578382915277327 1102 76-77 -4.1559532196310585 1102 78-79 -1.6764286034555838 1102 80-81 -1.2036804087161173 1102 82-83 -0.9602751568120667 1102 84-85 -0.972678591620511 1102 86-87 -0.6448382894903979 1102 88-89 -0.6712558646311315 1102 90-91 -0.7461508556841601 1102 92-93 -1.1606891812046136 1102 94-95 -4.825590856276712 1102 96-97 -1.8533376985376186 1102 98-99 -1.338000382114128 1102 100-101 -0.9843396225665266 1103 1 0.35358900605466204 1103 2 0.2954472207192147 1103 3 0.505298819713822 1103 4 0.30548280699697017 1103 5 0.3989547165847398 1103 6 0.36491565167418116 1103 7 0.46624310060360585 1103 8 0.2953707740660221 1103 9 0.7056968519866373 1103 10-11 0.8670682744578144 1103 12-13 0.5237862603193761 1103 14-15 0.8072276981872477 1103 16-17 0.7764940374926468 1103 18-19 0.7719066690713134 1103 20-21 0.8270979654361312 1103 22-23 0.8533065670688771 1103 24-25 0.8722162499893713 1103 26-27 0.8187351597015677 1103 28-29 0.868754547519373 1103 30-31 1.170776493022906 1103 32-33 1.0288131392975686 1103 34-35 1.2475514312023606 1103 36-37 1.2318621367745806 1103 38-39 1.2053704879673859 1103 40-41 1.4658020259640878 1103 42-43 1.0521493322989457 1103 44-45 1.1733055210901924 1103 46-47 1.1503689085078257 1103 48-49 1.2669549593023213 1103 50-51 1.1205485291894206 1103 52-53 1.1883863833388055 1103 54-55 1.1335726776442527 1103 56-57 1.14085941954092 1103 58-59 0.9083009429168563 1103 60-61 0.5999993168893418 1103 62-63 0.561987687048326 1103 64-65 0.4882247811471636 1103 66-67 -2.886265599242094 1103 68-69 -7.160489524433377 1103 70-71 -9.983627065778819 1103 72-73 -8.509181061304208 1103 74-75 -7.1168416026707 1103 76-77 -3.5101166386655436 1103 78-79 -0.8450270435020251 1103 80-81 0.10910908476227377 1103 82-83 0.3568050265451568 1103 84-85 0.27285957055579146 1103 86-87 0.30586973974646625 1103 88-89 0.6161982824361907 1103 90-91 0.5098455905432289 1103 92-93 0.476393781944072 1103 94-95 1.1165341580310404 1103 96-97 1.192633784072335 1103 98-99 0.6396912463531166 1103 100-101 0.5496239785170474 1104 1 0.3984285613208378 1104 2 0.01959083277663609 1104 3 0.33467515195747666 1104 4 0.1250774099784877 1104 5 0.4256554465611657 1104 6 0.4662586999717888 1104 7 0.5987853346316356 1104 8 0.30773151264102694 1104 9 0.6289201227639936 1104 10-11 0.5791885462270656 1104 12-13 0.5758471545038262 1104 14-15 0.7608252779469566 1104 16-17 0.8606241310501375 1104 18-19 0.7961770593813355 1104 20-21 0.9014308139239873 1104 22-23 0.5914953701851289 1104 24-25 0.6930360375525737 1104 26-27 0.8328621613817901 1104 28-29 1.0102573014341587 1104 30-31 1.0016894544207062 1104 32-33 0.7904530980875819 1104 34-35 0.9426313968531943 1104 36-37 0.9989171123837224 1104 38-39 0.8520800647751372 1104 40-41 0.9303193489400101 1104 42-43 1.2665913330820757 1104 44-45 1.0260241162494879 1104 46-47 0.8109132256035387 1104 48-49 0.7648273189350441 1104 50-51 0.7024257274972356 1104 52-53 0.767066196878293 1104 54-55 0.78452625096293 1104 56-57 0.839167237363899 1104 58-59 0.9010375702655296 1104 60-61 1.0195081033500806 1104 62-63 1.0144430212701039 1104 64-65 0.7097273206616954 1104 66-67 -2.4774390230899463 1104 68-69 -6.463167665650921 1104 70-71 -8.09112255491042 1104 72-73 -7.557539037501268 1104 74-75 -6.875068083809683 1104 76-77 -3.525100959236795 1104 78-79 -0.8402828737782748 1104 80-81 -0.199264204126262 1104 82-83 0.13294267376196345 1104 84-85 0.24932209057118726 1104 86-87 0.5059630038548484 1104 88-89 0.5356151024742637 1104 90-91 0.4620100739006894 1104 92-93 0.6300482341909266 1104 94-95 1.6505851491808237 1104 96-97 1.579655290674168 1104 98-99 1.6188715716321447 1104 100-101 1.5182493231090248 1105 1 0.5272986964757322 1105 2 0.42585821010699476 1105 3 0.5425290153251972 1105 4 0.330762758676002 1105 5 0.36119601035149174 1105 6 0.5126289775217003 1105 7 0.6412090681527118 1105 8 0.625399827958212 1105 9 0.887513588799699 1105 10-11 0.828051588054791 1105 12-13 0.8068647239940674 1105 14-15 0.9411287877933532 1105 16-17 1.0624654014133625 1105 18-19 1.009472761283078 1105 20-21 1.1377818842941494 1105 22-23 1.1903092930710244 1105 24-25 1.0476712743598924 1105 26-27 1.1380722336714868 1105 28-29 1.329631421007214 1105 30-31 1.1763576446651527 1105 32-33 0.9979017962447827 1105 34-35 1.2669820021641414 1105 36-37 1.1704302987497428 1105 38-39 1.1273325737166715 1105 40-41 1.4918301806502328 1105 42-43 1.7185058561409647 1105 44-45 1.372145011676345 1105 46-47 1.0896478065717012 1105 48-49 1.0631269756015662 1105 50-51 0.9922180891347807 1105 52-53 1.093059189281739 1105 54-55 1.3574040220140553 1105 56-57 1.1988831871915302 1105 58-59 1.7122652267253606 1105 60-61 1.6091591269207122 1105 62-63 1.606124568460988 1105 64-65 1.3492066187746232 1105 66-67 1.980309879000412 1105 68-69 -3.940938775492384 1105 70-71 -4.447513214032011 1105 72-73 -1.2146116072880382 1105 74-75 1.058092574765812 1105 76-77 1.4558741853455288 1105 78-79 1.1627385430198771 1105 80-81 1.2570548758081337 1105 82-83 1.4299129187812163 1105 84-85 1.4245299139003613 1105 86-87 1.622239547314102 1105 88-89 1.3952294674012826 1105 90-91 1.661563335216396 1105 92-93 1.659416839993952 1105 94-95 2.7850260123823674 1105 96-97 2.371297906773087 1105 98-99 2.105812920738348 1105 100-101 2.188821527953511 1106 1 0.11344028714806242 1106 2 0.19793423445777947 1106 3 0.14890687469100072 1106 4 0.24376755477185696 1106 5 0.40691664417795437 1106 6 0.30669262373524475 1106 7 0.3613789678956465 1106 8 0.3699628513292623 1106 9 0.39817943097278885 1106 10-11 0.6524445924215456 1106 12-13 0.6265722474184869 1106 14-15 0.7780379744367991 1106 16-17 0.6373803233359254 1106 18-19 0.522704809913094 1106 20-21 0.3356680958316076 1106 22-23 0.5068501290574332 1106 24-25 0.6673968658813649 1106 26-27 0.8386385462710351 1106 28-29 0.9317191508611344 1106 30-31 0.8371109352353372 1106 32-33 0.9089378853306371 1106 34-35 1.003104898617245 1106 36-37 0.8589610523802094 1106 38-39 0.8757486336908258 1106 40-41 0.804884112429157 1106 42-43 0.9524734015452623 1106 44-45 1.0642384004821182 1106 46-47 1.2107070383257472 1106 48-49 1.0857890204927365 1106 50-51 0.9456760426455091 1106 52-53 1.330908095863716 1106 54-55 1.4201235747362269 1106 56-57 1.4048973590197278 1106 58-59 1.5950002953513298 1106 60-61 1.5209355309400578 1106 62-63 1.2649816033014076 1106 64-65 1.4063188001902418 1106 66-67 1.931077892386309 1106 68-69 -4.592105241104264 1106 70-71 -5.621697123407685 1106 72-73 -1.8553501571533033 1106 74-75 1.080017286257661 1106 76-77 1.324295206592538 1106 78-79 1.2478297392054216 1106 80-81 1.448856670572443 1106 82-83 1.2759959879009308 1106 84-85 1.3207832584363146 1106 86-87 1.3117732890381042 1106 88-89 1.2469383933393772 1106 90-91 1.038462348865263 1106 92-93 1.2301424082594572 1106 94-95 2.3125470635043506 1106 96-97 2.616170115237182 1106 98-99 2.30081628689042 1106 100-101 2.1988436569000918 1107 1 0.09415861081646781 1107 2 0.11328110060976826 1107 3 0.034604677841127085 1107 4 -0.04626664289410343 1107 5 -0.02887111411931187 1107 6 0.16447203312038283 1107 7 0.07720192797960834 1107 8 0.01955157754775172 1107 9 0.11874798076022586 1107 10-11 0.34608863609887663 1107 12-13 0.12501969768732835 1107 14-15 0.2129261812626453 1107 16-17 0.01573205595584426 1107 18-19 0.10489305121161863 1107 20-21 0.005233708086230138 1107 22-23 0.20747473052703214 1107 24-25 0.3414823005665113 1107 26-27 0.3686739079495638 1107 28-29 0.1533477835237207 1107 30-31 0.23328037453303807 1107 32-33 0.3046238117151603 1107 34-35 0.557026821514846 1107 36-37 0.6144685266839929 1107 38-39 0.36670675034407196 1107 40-41 0.29743155393592957 1107 42-43 0.7293197205473803 1107 44-45 0.5941522443650129 1107 46-47 0.7475202149480289 1107 48-49 0.751681597482257 1107 50-51 0.8749223061331861 1107 52-53 0.5971590224369088 1107 54-55 0.8337989316307919 1107 56-57 1.1140109378659098 1107 58-59 0.6338373828383901 1107 60-61 0.7409687628593282 1107 62-63 0.9644284415042996 1107 64-65 0.9175185837606534 1107 66-67 1.6935221141191903 1107 68-69 -4.834942246726705 1107 70-71 -5.604701260768842 1107 72-73 -2.094816003297705 1107 74-75 0.48190157957091984 1107 76-77 0.7782658645218632 1107 78-79 1.0530974716897497 1107 80-81 1.006880458640289 1107 82-83 1.0561079377690845 1107 84-85 1.1230603740131677 1107 86-87 1.2968198847170633 1107 88-89 1.2061010589793248 1107 90-91 1.2092693613374657 1107 92-93 1.1131501911544852 1107 94-95 2.044147190650339 1107 96-97 1.99154690034959 1107 98-99 1.5963063137970188 1107 100-101 1.6249873791948346 1108 1 0.017893275106040107 1108 2 -0.01413237437456516 1108 3 0.1566196037193741 1108 4 -0.09954347285740539 1108 5 0.0596438381560489 1108 6 0.23912646776856405 1108 7 0.20783585139006533 1108 8 0.18222551086727634 1108 9 0.23340894023456116 1108 10-11 0.25767216538005044 1108 12-13 0.25353609841994995 1108 14-15 0.16484958359398405 1108 16-17 -0.03288060626798739 1108 18-19 0.204714769136082 1108 20-21 1.417716613190123E-4 1108 22-23 0.19408969945288845 1108 24-25 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2311 8 -0.20618561044857842 2311 9 0.08198445070370042 2311 10-11 -1.7490374539829858 2311 12-13 -0.45077679759370426 2311 14-15 -0.14165918784757991 2311 16-17 -0.04231999472548864 2311 18-19 0.03069140954872296 2311 20-21 0.15739920025129805 2311 22-23 0.09179641510232273 2311 24-25 0.016949578315568203 2311 26-27 0.11109204604237988 2311 28-29 -0.23751633292970808 2311 30-31 -0.001064908752404392 2311 32-33 0.25208407816322875 2311 34-35 0.3909710819825065 2311 36-37 0.4459213378027087 2311 38-39 0.48709277556054076 2311 40-41 0.46301958876886573 2311 42-43 0.21219823531908588 2311 44-45 0.29155040894428197 2311 46-47 0.14707393539336167 2311 48-49 0.26020158574500485 2311 50-51 0.09824800093665687 2311 52-53 0.6119190841040627 2311 54-55 0.43854615373657424 2311 56-57 0.5876537403369433 2311 58-59 0.5782472831183121 2311 60-61 0.8363145383020765 2311 62-63 0.8004705807273744 2311 64-65 0.7593688002632959 2311 66-67 1.5556043432036688 2311 68-69 4.04897771638479 2311 70-71 4.3208867274876965 2311 72-73 2.6328094696468405 2311 74-75 1.8303671976475293 2311 76-77 1.2838585259440265 2311 78-79 1.1680061120780145 2311 80-81 0.9606071917870089 2311 82-83 0.6288816245862563 2311 84-85 0.6529247745826439 2311 86-87 0.7584486925319602 2311 88-89 0.911968365916259 2311 90-91 1.189745698196635 2311 92-93 0.895174572348175 2311 94-95 1.4764053077527244 2311 96-97 1.4751919850642992 2311 98-99 1.09540661297277 2311 100-101 1.010582928353898 2312 1 -0.19825242597567438 2312 2 -0.1978372818619114 2312 3 -0.14152845038317707 2312 4 0.023435722375609203 2312 5 -0.11353595633551095 2312 6 0.14307581857180907 2312 7 -0.04521969005865145 2312 8 0.06187018779243658 2312 9 -1.5524196108801647 2312 10-11 -1.1930656928631436 2312 12-13 -2.045575805495538 2312 14-15 -1.9966000211323802 2312 16-17 0.02007929080851767 2312 18-19 -2.3143612046590505 2312 20-21 -2.083486747289804 2312 22-23 -0.7113662618153782 2312 24-25 -1.4788750183411992 2312 26-27 -2.0100444014307755 2312 28-29 -0.023319718877253592 2312 30-31 0.19099151076338927 2312 32-33 0.18941783862297257 2312 34-35 0.30593895042757424 2312 36-37 0.3965336332188656 2312 38-39 0.18450162290167782 2312 40-41 0.5012182802088674 2312 42-43 0.7230899587741533 2312 44-45 0.8366959716833193 2312 46-47 0.608634985347738 2312 48-49 0.9245930762220276 2312 50-51 0.9815150811393423 2312 52-53 1.0810640546905823 2312 54-55 1.071953991506195 2312 56-57 1.0201289378829053 2312 58-59 0.950011072506161 2312 60-61 0.9947784364130143 2312 62-63 0.5473860485278692 2312 64-65 1.1158239908199192 2312 66-67 2.338877733228326 2312 68-69 4.1963436083994665 2312 70-71 4.866908384738167 2312 72-73 2.7331659745879158 2312 74-75 1.9921024957623494 2312 76-77 1.1925274932688552 2312 78-79 1.108436539010274 2312 80-81 1.321871194738062 2312 82-83 1.1854503919382644 2312 84-85 1.242618893828574 2312 86-87 1.0887581650308427 2312 88-89 1.1635094185887134 2312 90-91 1.3653310555267062 2312 92-93 1.7972776845465148 2312 94-95 2.4817273998955436 2312 96-97 2.196377940371349 2312 98-99 2.0885398278755964 2312 100-101 2.2016249226459372 2313 1 -0.11916488754195953 2313 2 -0.13633731200599897 2313 3 -0.14197458289659082 2313 4 0.02228120377670706 2313 5 -0.1180907281447574 2313 6 0.27076014967448003 2313 7 -3.4208421654912193 2313 8 -1.229101657628675 2313 9 -0.8130001860293774 2313 10-11 0.06923987776445983 2313 12-13 -1.5356365458343646 2313 14-15 -0.5295271628698828 2313 16-17 0.03859981893295128 2313 18-19 0.3314050580768253 2313 20-21 0.32448711778524597 2313 22-23 -1.2735866753922735 2313 24-25 -2.9711860149047737 2313 26-27 -2.1547767219227225 2313 28-29 -0.485415034485861 2313 30-31 0.08640659486540159 2313 32-33 -2.4720610303708206 2313 34-35 -2.6037162011971944 2313 36-37 -0.807815405184698 2313 38-39 -0.43417791529088845 2313 40-41 -0.3108182544266924 2313 42-43 -0.2436249384345075 2313 44-45 0.27568659687172214 2313 46-47 0.09142421787923638 2313 48-49 0.15106787576383596 2313 50-51 0.46397800391019217 2313 52-53 0.5482160411860306 2313 54-55 0.8214483244175135 2313 56-57 0.7471591422598287 2313 58-59 0.6885902308832037 2313 60-61 1.226194605702215 2313 62-63 0.8993951520426684 2313 64-65 0.4917456463332712 2313 66-67 1.9699615639391226 2313 68-69 5.01718070974389 2313 70-71 4.975461016317116 2313 72-73 3.205685869686487 2313 74-75 2.5133932656423745 2313 76-77 1.7374565039398604 2313 78-79 1.3584907983700134 2313 80-81 1.3816846028287344 2313 82-83 1.0950279225345163 2313 84-85 1.190287248564207 2313 86-87 1.2385953869516264 2313 88-89 1.084250058849154 2313 90-91 1.157467969977784 2313 92-93 1.737750645298913 2313 94-95 2.528367841205 2313 96-97 2.7310994089913514 2313 98-99 2.5488212229440244 2313 100-101 2.1388860307426363 2314 1 -0.3414105015770481 2314 2 -0.3113080722399175 2314 3 -0.21589271155156453 2314 4 -0.5351690886209539 2314 5 -1.819962073173997 2314 6 -3.152374353249499 2314 7 -1.0990409959005802 2314 8 -0.22507826581581014 2314 9 -0.20850895795920366 2314 10-11 -0.02349696434080073 2314 12-13 -0.14571841717938838 2314 14-15 -2.9485330108230983 2314 16-17 -3.221645795102134 2314 18-19 -1.0645364623910183 2314 20-21 -0.3498286716884351 2314 22-23 -0.34578550580162926 2314 24-25 -2.5445193482381043 2314 26-27 -1.5990925113964138 2314 28-29 -0.7382922274683139 2314 30-31 -0.18422498408197185 2314 32-33 -0.287558106394215 2314 34-35 -0.6915524585071395 2314 36-37 -0.18424815372271297 2314 38-39 -1.9393592018405919 2314 40-41 -4.8307129912688005 2314 42-43 -6.620174646036848 2314 44-45 -3.0782198358768156 2314 46-47 -3.5654646710096536 2314 48-49 -1.419821013125052 2314 50-51 -1.2700570838091068 2314 52-53 -3.046544192732096 2314 54-55 -1.758387932892429 2314 56-57 -4.270139103354207 2314 58-59 -4.869526728181125 2314 60-61 -2.618156271490765 2314 62-63 -1.9041019239807255 2314 64-65 -0.7259268682866136 2314 66-67 -0.6376875588678956 2314 68-69 1.305192405650324 2314 70-71 1.1397417180715017 2314 72-73 -0.6989457092608795 2314 74-75 -0.17665351798335394 2314 76-77 -0.4800171802706643 2314 78-79 -0.09280744724402368 2314 80-81 0.15601208820885049 2314 82-83 -0.13484342249472192 2314 84-85 -0.35935017833637417 2314 86-87 -0.07417654287293374 2314 88-89 0.15316233955091008 2314 90-91 0.4293276191005866 2314 92-93 0.8116453821410197 2314 94-95 0.825139771029562 2314 96-97 1.1472748475878447 2314 98-99 0.4295346732364216 2314 100-101 0.3962778436081358 2315 1 -0.23462149172738478 2315 2 -0.25590112563546086 2315 3 -0.1349388494468471 2315 4 -0.3066175074908344 2315 5 -0.9471886154238689 2315 6 -0.2801898016683708 2315 7 -0.1712919031063862 2315 8 -0.08402694388735199 2315 9 0.15777823747885122 2315 10-11 -1.5585710960152497 2315 12-13 -1.1187738863343881 2315 14-15 0.13288223023444345 2315 16-17 -1.430667049638167 2315 18-19 -2.7029532586585105 2315 20-21 -1.792549252300148 2315 22-23 -1.0319679837694906 2315 24-25 -0.7234929096688987 2315 26-27 -0.27461246992414345 2315 28-29 -0.4328189252391823 2315 30-31 -2.9071270058549104 2315 32-33 -2.2637374739421645 2315 34-35 -1.137700721856021 2315 36-37 -4.567695535785958 2315 38-39 -1.805396526879477 2315 40-41 -1.261351664156308 2315 42-43 -1.2661518362908666 2315 44-45 -1.5447403128078676 2315 46-47 -1.2561479265824858 2315 48-49 -4.527452946769426 2315 50-51 -4.001067970278783 2315 52-53 -2.406370009217323 2315 54-55 -3.7133138012179856 2315 56-57 -6.209308621239121 2315 58-59 -7.012418381887709 2315 60-61 -5.563197225352869 2315 62-63 -6.278336242280361 2315 64-65 -5.981355587830418 2315 66-67 -3.929449093341713 2315 68-69 -1.564144038102917 2315 70-71 0.8125732369932237 2315 72-73 -0.881419529893332 2315 74-75 -2.363018473919073 2315 76-77 -4.784313706968433 2315 78-79 -3.5333320714016168 2315 80-81 -3.316299990588451 2315 82-83 -6.534121805076371 2315 84-85 -4.590759198554107 2315 86-87 -4.830415008399115 2315 88-89 -3.2465349129115033 2315 90-91 -3.035634537457213 2315 92-93 -3.727475405832024 2315 94-95 -1.9799675384572204 2315 96-97 -0.8685540793172919 2315 98-99 -2.4528385771523773 2315 100-101 -1.7424396265836748 2316 1 -0.3187255387411412 2316 2 -0.3465968086607276 2316 3 -1.2422163250449891 2316 4 -0.4094080823316446 2316 5 -0.458052410509616 2316 6 -2.528609916428458 2316 7 -0.7171839347227618 2316 8 -0.5554796380285012 2316 9 -0.20164332045205668 2316 10-11 -0.2755255521052433 2316 12-13 -0.8958293377054005 2316 14-15 -1.2438160296910183 2316 16-17 -1.5829162353537072 2316 18-19 -0.5399429804013067 2316 20-21 -4.827614835210447 2316 22-23 -4.998058804829647 2316 24-25 -3.597658284773267 2316 26-27 -2.6255750728601086 2316 28-29 -1.9101630107730614 2316 30-31 -0.7269453957457799 2316 32-33 -2.1334986438441845 2316 34-35 -2.9035135791475 2316 36-37 -5.426540892430548 2316 38-39 -2.8532905912059476 2316 40-41 -2.6962715961858947 2316 42-43 -4.263851032543421 2316 44-45 -2.103010001685849 2316 46-47 -1.8685338533995868 2316 48-49 -1.1970548610381435 2316 50-51 -1.0757860718022485 2316 52-53 -2.9295767827835526 2316 54-55 -1.7913850741159827 2316 56-57 -0.7963243520677565 2316 58-59 -0.16722712840982368 2316 60-61 -1.503064218889282 2316 62-63 -0.4940035820710591 2316 64-65 0.03194505852871288 2316 66-67 1.2465617264379958 2316 68-69 3.5890974942723943 2316 70-71 3.682195691770758 2316 72-73 1.7445351369369462 2316 74-75 0.7639765563448329 2316 76-77 -0.4137095759253242 2316 78-79 0.31512165510017454 2316 80-81 -0.21293588205987035 2316 82-83 -0.22938201597671437 2316 84-85 0.006042617546984985 2316 86-87 -0.5235784868409148 2316 88-89 0.03949967517126396 2316 90-91 0.11236249617320127 2316 92-93 0.3473320602427883 2316 94-95 1.0534347967585482 2316 96-97 1.3523897704008796 2316 98-99 0.8598159768384832 2316 100-101 0.2527867058151081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 30.0 4 68.0 5 241.0 6 568.0 7 1183.0 8 2114.0 9 3402.0 10 5240.0 11 7208.0 12 9085.0 13 11303.0 14 13819.0 15 17267.0 16 20474.0 17 22570.0 18 19057.0 19 15225.0 20 14518.0 21 14391.0 22 15421.0 23 17144.0 24 20350.0 25 24864.0 26 30688.0 27 37711.0 28 44826.0 29 52595.0 30 60190.0 31 68919.0 32 78759.0 33 88687.0 34 100096.0 35 106306.0 36 94505.0 37 66278.0 38 20761.0 39 781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57661103719558 22.098433794180597 16.44479956036328 18.880155608260544 2 16.376906816232577 24.001460472791916 38.06221650243879 21.559416208536717 3 19.808340487055528 28.585731712827002 31.299868973930327 20.306058826187144 4 12.821307549812497 19.58171056793024 36.610129670627565 30.986852211629696 5 11.777989226936453 40.73830094275601 32.85007260658077 14.633637223726764 6 28.187141372611034 38.69850449554963 17.18049970632088 15.933854425518456 7 25.229476347535357 37.1526679618669 20.24126869380563 17.37658699679212 8 26.51922468711878 34.75172818867754 19.261193692676095 19.467853431527583 9 26.91600777120137 17.421949125739847 20.735462883477158 34.92658021958162 10-11 24.399674693895992 29.164957303573846 25.56241810870646 20.8729498938237 12-13 26.532779108119097 27.20245786834139 27.306465458817154 18.958297564722358 14-15 23.099941264175666 27.391451678489133 28.286811548366693 21.22179550896851 16-17 19.316096280024254 30.890654382111006 28.864940672138452 20.928308665726288 18-19 19.068120925146957 27.637522760440437 34.25203432025916 19.042321994153447 20-21 20.719649392310124 26.28360366872995 34.51664934712872 18.480097591831203 22-23 19.972484525369357 26.69046220575611 34.385442551845664 18.95161071702887 24-25 19.518863235892105 27.026295576740612 34.81782858175575 18.63701260561153 26-27 19.58112131713422 27.047969312652203 34.83352836727225 18.537381002941324 28-29 19.03656489291274 27.14758587654745 35.10112227536982 18.71472695516999 30-31 19.0566450357087 27.415906299786823 34.88305998682442 18.644388677680052 32-33 19.03685622700609 27.47706144270941 34.4822569028045 19.00382542748 34-35 19.457775604827503 27.26019839417569 34.49309533503022 18.788930665966582 36-37 19.66466585983136 27.10410346306015 34.41767656758512 18.81355410952337 38-39 19.58595031566607 27.185363984986243 34.501291022003684 18.727394677344 40-41 19.348869398444883 27.380279269213894 34.45573261804711 18.815118714294115 42-43 19.23391949605775 27.755312302271278 33.87987681853891 19.130891383132067 44-45 19.284820757456114 27.984232553553873 33.3980511392776 19.33289554971241 46-47 19.564380705574813 28.241716245949423 32.505195366117846 19.68870768235792 48-49 19.773464993313127 28.37978715080489 32.09187132442565 19.754876531456336 50-51 19.92023890338894 28.829593193985083 31.658633722626217 19.591534179999755 52-53 20.044681996844858 29.033395745850683 31.33341984573362 19.588502411570836 54-55 19.48533002029058 28.90137006615235 31.621474014677215 19.99182589887986 56-57 19.940139490715612 29.307016524917547 30.874806834150142 19.878037150216706 58-59 20.025685078523857 28.75196908391393 31.011704695705927 20.210641141856282 60-61 20.02074923161872 28.805147906053698 31.35262216607157 19.82148069625601 62-63 20.330192408918087 28.64889043512835 31.300957187490823 19.71995996846274 64-65 20.149350168173694 28.743513881333172 31.375734912714147 19.731401037778987 66-67 20.074560175299013 28.68115903220638 31.501583291767293 19.74269750072731 68-69 20.915366890527128 28.07648626575956 31.65510904994203 19.353037793771282 70-71 20.66295585114949 28.129021779941432 31.76734546214343 19.440676906765646 72-73 20.39680712605091 28.680034658652154 30.801245681263662 20.121912534033275 74-75 19.789072958273483 29.277667203397424 30.757840296803963 20.17541954152513 76-77 19.796345994905487 29.196161356078644 30.80359462785064 20.20389802116523 78-79 19.606291776598646 29.167764291985353 30.859274568695376 20.36666936272062 80-81 19.36874312950042 29.59371824547151 30.699007394950538 20.33853123007753 82-83 19.522839199385533 29.630054669498033 30.23720236750553 20.609903763610898 84-85 19.460985858220756 29.29313694483351 30.227126914561964 21.01875028238377 86-87 19.699343782926064 29.195379552268747 30.247929328800723 20.857347336004466 88-89 19.793853745770424 28.974559511820225 30.222705365739493 21.008881376669862 90-91 19.33335923832107 29.235736593265184 30.51952452901118 20.911379639402572 92-93 19.335991859956554 29.443543810566876 30.38274831289286 20.837716016583713 94-95 19.19277970033474 29.145872933704165 30.50384579326874 21.157501572692354 96-97 19.116322970204116 28.961627858526168 30.3644637846044 21.557585386665316 98-99 19.176705511600385 28.892623408994268 30.116625176740758 21.81404590266459 100-101 19.239929566041326 28.826457648125714 30.381038318007427 21.552574467825533 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10773.0 1 7218.0 2 3728.0 3 3894.5 4 3584.0 5 3225.5 6 4397.5 7 7550.0 8 10489.5 9 11295.0 10 9633.0 11 7026.0 12 5565.0 13 4930.0 14 4615.0 15 4341.0 16 4082.0 17 4002.5 18 4158.5 19 4316.5 20 4419.0 21 4878.0 22 5417.0 23 5906.0 24 6555.0 25 7469.0 26 8782.0 27 10391.5 28 11938.0 29 13527.5 30 15169.0 31 17038.0 32 19353.0 33 21815.5 34 24189.5 35 25885.0 36 28000.0 37 29942.5 38 30589.0 39 30716.5 40 30643.0 41 31110.5 42 31973.5 43 32942.0 44 33973.0 45 34567.5 46 35025.0 47 37134.0 48 42919.5 49 42836.0 50 36601.0 51 32605.0 52 29669.0 53 27930.5 54 26515.5 55 24540.5 56 22094.0 57 19895.0 58 18322.5 59 16716.0 60 14415.0 61 11783.5 62 9290.5 63 6969.0 64 5102.0 65 3814.5 66 2847.0 67 2141.0 68 1575.0 69 1124.5 70 842.0 71 694.0 72 608.5 73 557.5 74 458.0 75 355.5 76 255.5 77 143.0 78 90.0 79 67.0 80 37.5 81 22.0 82 13.0 83 5.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02548231148059459 2 0.014367686260335246 3 0.0 4 0.0 5 9.036280666877514E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.710884200063254E-4 18-19 0.001355442100031627 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.5181403334387566E-4 28-29 0.017394840283739214 30-31 0.005150679980120183 32-33 0.009668820313558939 34-35 0.010436904170243528 36-37 0.09903763610897756 38-39 0.1021551529390503 40-41 0.11430895043600055 42-43 0.10179370171237519 44-45 0.09768219400894591 46-47 0.0991731803189807 48-49 0.10170333890570642 50-51 0.09926354312564949 52-53 0.07549812497176162 54-55 0.06488049518818055 56-57 0.03718429494420097 58-59 0.015452039940360547 60-61 0.009307369086883838 62-63 0.0020331631500474408 64-65 0.005060317173451407 66-67 0.4508652238738535 68-69 0.8618804500067773 70-71 0.9636741517191525 72-73 0.29982379252699587 74-75 0.1638277684904893 76-77 0.014955044503682286 78-79 0.008539285230199251 80-81 0.0014909863100347896 82-83 0.0 84-85 0.0 86-87 3.6145122667510056E-4 88-89 7.680838566845886E-4 90-91 0.0037952378800885553 92-93 0.0019879817467130527 94-95 0.23959698188225725 96-97 0.24144941941896714 98-99 0.01414177924366331 100-101 0.007364568743505173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1106650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.32062574486625 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76703720730525 40.1649942326564 2 12.87331287260752 13.470795698085341 3 4.374167660396237 6.865775673146662 4 2.0495830080817647 4.2894186199553275 5 1.1121698168524714 2.9094710376137294 6 0.6707808145274916 2.1057403172237654 7 0.40692658944405363 1.4903457654356018 8 0.2808397828813281 1.175497053952277 9 0.20832780139315799 0.9809856836048018 >10 1.1141460130858805 10.652561425186432 >50 0.09166415689506728 3.2827537914278446 >100 0.046166325966163664 4.224669194124629 >500 0.002264762761670999 0.8199771295917424 >1k 0.001916337721413922 2.4464413248379686 >5k 0.0 0.0 >10k+ 6.968500805141534E-4 5.1205730531575036 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17187 1.5530655582162383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14889 1.345411828491393 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12250 1.1069443816924953 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11852 1.0709799846383228 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4390 0.3966927212759228 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4348 0.39289748339583425 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3460 0.31265531107396194 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2725 0.24623864817241223 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2342 0.21162969321827135 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2320 0.2096417114715583 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2239 0.20232232413138754 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1736 0.15686983237699362 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1224 0.11060407536258075 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.036280666877513E-5 0.0 14-15 0.0 0.0 0.0 2.2590701667193783E-4 0.0 16-17 0.0 0.0 0.0 4.5181403334387566E-4 0.0 18-19 0.0 0.0 0.0 8.132652600189762E-4 0.0 20-21 0.0 0.0 0.0 9.036280666877513E-4 0.0 22-23 0.0 0.0 0.0 0.0018072561333755026 0.0 24-25 0.0 0.0 0.0 0.0023042515700537657 0.0 26-27 0.0 0.0 0.0 0.003750056476754168 0.0 28-29 0.0 0.0 0.0 0.005918763836804772 0.0 30-31 0.0 0.0 0.0 0.011476076446934443 0.0 32-33 0.0 0.0 0.0 0.020602719920480732 0.0 34-35 0.0 0.0 0.0 0.034021596710793835 0.0 36-37 0.0 0.0 0.0 0.0534044187412461 0.0 38-39 0.0 0.0 0.0 0.08263678669859487 0.0 40-41 0.0 0.0 0.0 0.1195499932227895 0.0 42-43 0.0 0.0 0.0 0.15637283694031537 0.0 44-45 0.0 0.0 0.0 0.1980752722179551 0.0 46-47 0.0 0.0 0.0 0.2511182397325261 0.0 48-49 0.0 0.0 0.0 0.3147336556273438 0.0 50-51 0.0 0.0 0.0 0.3839967469389599 0.0 52-53 0.0 0.0 0.0 0.4514074007138662 0.0 54-55 0.0 0.0 0.0 0.5208963990421542 0.0 56-57 0.0 0.0 0.0 0.5957168029639001 0.0 58-59 0.0 0.0 0.0 0.6747842587990783 0.0 60-61 0.0 9.036280666877513E-5 0.0 0.7586409433877016 0.0 62-63 0.0 9.036280666877513E-5 0.0 0.8425428093796593 0.0 64-65 0.0 9.036280666877513E-5 0.0 0.9248181448515791 0.0 66-67 0.0 9.036280666877513E-5 0.0 1.0108887182035873 0.0 68-69 0.0 9.036280666877513E-5 0.0 1.120318077079474 0.0 70-71 0.0 9.036280666877513E-5 0.0 1.2709076943929878 0.0 72-73 0.0 9.036280666877513E-5 0.0 1.437401165680206 0.0 74-75 0.0 9.036280666877513E-5 0.0 1.6218768354945103 0.0 76-77 0.0 9.036280666877513E-5 0.0 1.814982153345683 0.0 78-79 0.0 9.036280666877513E-5 0.0 2.0445940450910403 0.0 80-81 0.0 9.036280666877513E-5 0.0 2.2982424434102926 0.0 82-83 0.0 9.036280666877513E-5 0.0 2.56955677043329 0.0 84-85 0.0 9.036280666877513E-5 0.0 2.835991505896173 0.0 86-87 0.0 9.036280666877513E-5 0.0 3.1127727827226312 0.0 88-89 0.0 9.036280666877513E-5 0.0 3.4067229928161566 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGTA 35 2.824108E-4 54.14405 7 GGTATCA 10630 0.0 31.168694 1 GTATCAA 14960 0.0 26.868225 1 GGGACCG 120 1.7413362E-4 23.688023 5 GTACATG 9875 0.0 23.519804 1 TACATGG 9920 0.0 23.309116 2 GAGTACT 11595 0.0 21.533682 12-13 ACATGGG 10240 0.0 21.513535 3 TATCAAC 18655 0.0 21.51324 2 AGTACTT 11675 0.0 21.223804 12-13 ATCAACG 18915 0.0 21.164555 3 TCAACGC 19135 0.0 20.921223 4 CAACGCA 19430 0.0 20.579199 5 GTACTTT 12235 0.0 20.368553 14-15 AACGCAG 19685 0.0 20.288546 6 CATGGGA 7505 0.0 19.75843 4 CATGGGG 2535 0.0 19.623213 4 ACTTTTT 13475 0.0 19.074335 16-17 TACTTTT 13035 0.0 18.973083 14-15 CATATAG 355 8.185452E-11 18.68351 3 >>END_MODULE