FastQCFastQC Report
Fri 27 May 2016
SRR939368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080978
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125971.1653336145601483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.8035316167396561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83450.7719861088754814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.647561745012387No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26380.2440382690489538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24520.22683162839576754No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.21397290231623586No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.18233488563134495No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17370.160687821583788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.14533135734492283No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA15370.14218605744057694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA123200.032.0574341
GGTATCA84350.031.8463861
GTACATG90150.028.9666921
TACATGG90900.027.5580982
TATCAAC155100.025.48352
ATCAACG159000.025.2422123
ACATGGG96150.025.1952863
TCAACGC159700.025.0145844
CAACGCA160750.024.6921145
AACGCAG164000.024.094826
GAGTACT89000.023.78114912-13
CATGGGG24050.023.389514
CCGAGTA1251.9957639E-423.2159659
AGTACTT90600.022.93738612-13
CATGGGA72750.022.9344984
CGCAGAG179100.021.9013428
ACGCAGA179900.021.8308357
GTACTTT98350.021.64514214-15
ATGGGAT28900.021.2716545
GCAGAGT176800.020.9825659
Produced by FastQC (version 0.11.5)