FastQCFastQC Report
Fri 27 May 2016
SRR939368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080978
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165201.528245718229233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140281.2977137370048235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA122581.1339731243374056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113101.046274762298585No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.38418913243377756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41130.3804887796051354No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.2940855410563397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.2254439960850267No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA21660.20037410567097574No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21450.1984314204359386No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20590.19047566185435783No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.14930923663571322No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11730.10851284669993284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1203.8398866E-935.580322
GGTATCA97900.033.5438351
ATCACGC1001.3133231E-633.2036863
CAACGCG1004.9938106E-528.4655724
AACGCGG1056.9910835E-527.0937635
GTATCAA136950.026.5780221
GTACATG91500.023.2870561
CATTCCG2052.7206625E-823.1288229
GAGTACG1252.270043E-422.7787841
TACATGG92550.022.6053242
GTATAGG3301.8189894E-1221.5708181
AGTACTT110500.021.38601912-13
GAGTACT108050.021.36639412-13
TATCAAC174950.020.825522
ACATGGG94300.020.8246143
TAAGGTG3900.020.6676795
ATCAACG178000.020.3592413
GTACTTT115950.020.13550414-15
TCAACGC181250.019.9717084
CATGGGA69150.019.8963994
Produced by FastQC (version 0.11.5)