##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939368_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1080978 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.61344726719693 31.0 30.0 31.0 28.0 34.0 2 30.84328358208955 31.0 31.0 33.0 27.0 34.0 3 30.795016179792743 31.0 30.0 34.0 27.0 34.0 4 34.75264158937555 35.0 35.0 37.0 32.0 37.0 5 34.3329549722566 35.0 35.0 37.0 30.0 37.0 6 34.43962689342429 35.0 35.0 37.0 30.0 37.0 7 34.262250480583326 35.0 35.0 37.0 30.0 37.0 8 34.177956443146854 35.0 35.0 37.0 30.0 37.0 9 35.581288425851405 37.0 35.0 39.0 30.0 39.0 10-11 35.367428846840546 37.0 34.5 39.0 29.5 39.0 12-13 35.332904092405215 37.0 34.0 39.0 30.0 39.0 14-15 35.9379094671677 38.0 34.0 40.0 28.5 41.0 16-17 35.891563473077156 38.0 34.0 40.0 28.5 41.0 18-19 36.00247877385109 38.0 34.0 40.0 29.0 41.0 20-21 35.9373548767875 38.0 34.0 40.0 28.5 41.0 22-23 35.739188494122914 38.0 34.0 40.0 27.5 41.0 24-25 35.53719594663351 38.0 34.0 40.0 27.0 41.0 26-27 34.97878772740981 37.0 33.0 40.0 26.5 41.0 28-29 34.695682983372464 37.0 33.0 39.5 25.5 40.5 30-31 34.277652736688445 36.5 33.0 39.0 25.0 40.0 32-33 33.822458458914056 36.0 32.0 39.0 24.0 40.0 34-35 33.83875435022729 36.0 32.0 39.0 24.0 40.0 36-37 33.5477373267541 36.0 32.0 39.0 23.5 40.0 38-39 33.27419660714649 36.0 31.0 39.0 22.0 40.0 40-41 33.028111117895094 35.5 31.0 39.0 21.5 40.0 42-43 32.733762389243815 35.0 31.0 39.0 20.0 40.0 44-45 32.203802482566715 35.0 30.5 39.0 17.0 40.0 46-47 31.576267509607042 35.0 30.0 39.0 13.5 40.0 48-49 31.032017302849827 35.0 29.5 39.0 8.5 40.0 50-51 30.72633948146956 35.0 28.0 38.5 8.0 40.0 52-53 30.55389656403738 35.0 28.0 38.0 6.5 40.0 54-55 30.186693901263485 34.5 27.5 38.0 2.0 40.0 56-57 29.68061745937475 34.0 26.5 38.0 2.0 40.0 58-59 29.21023832122393 34.0 26.0 38.0 2.0 40.0 60-61 28.832426746890317 34.0 26.0 38.0 2.0 40.0 62-63 28.59854409617957 33.0 26.0 37.0 2.0 39.5 64-65 28.105187617139293 33.0 25.5 37.0 2.0 39.0 66-67 27.160063849588056 32.5 23.0 36.0 2.0 39.0 68-69 23.774684128631662 29.0 10.0 35.0 2.0 38.0 70-71 22.455395484459444 27.0 8.0 34.0 2.0 37.0 72-73 23.432694282399826 28.5 10.5 34.0 2.0 37.0 74-75 24.130349091285854 29.0 14.0 34.0 2.0 36.0 76-77 23.91993176549384 29.5 15.5 33.5 2.0 36.0 78-79 25.030965477558286 31.0 20.0 34.0 2.0 36.0 80-81 25.043743720963796 31.0 19.5 34.0 2.0 36.0 82-83 24.69358812112735 31.0 18.0 34.0 2.0 35.5 84-85 24.312865294205803 31.0 16.5 34.0 2.0 35.0 86-87 23.81554434965374 30.0 10.0 34.0 2.0 35.0 88-89 23.246702523085574 30.0 6.0 34.0 2.0 35.0 90-91 22.561639089787214 29.0 2.0 34.0 2.0 35.0 92-93 21.939373881799632 29.0 2.0 34.0 2.0 35.0 94-95 21.021775651308353 29.0 2.0 33.0 2.0 35.0 96-97 20.280935412191553 27.5 2.0 33.0 2.0 35.0 98-99 19.403435130039647 26.5 2.0 33.0 2.0 34.0 100-101 18.121897485425237 24.0 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25679366749749377 1101 2 0.25772315766787557 1101 3 0.5092371963731566 1101 4 0.3925831723907791 1101 5 0.6431912302170559 1101 6 0.5958669106103045 1101 7 0.6356291576484239 1101 8 0.8927434625707207 1101 9 0.7000850818257476 1101 10-11 0.8282132051169739 1101 12-13 0.9095503383886978 1101 14-15 0.8641924392374634 1101 16-17 0.9771112961927031 1101 18-19 0.7083210156208608 1101 20-21 0.8831838725679191 1101 22-23 0.8458755872380195 1101 24-25 0.890572598214554 1101 26-27 0.8071316209122656 1101 28-29 0.6996580940208545 1101 30-31 0.5098827818441265 1101 32-33 0.9973541759396625 1101 34-35 1.1944781543432157 1101 36-37 1.2799886134820042 1101 38-39 1.3295122706104223 1101 40-41 1.4331021187077368 1101 42-43 1.3898874866563347 1101 44-45 1.5541703262447726 1101 46-47 1.5428771078629957 1101 48-49 1.4472276060121168 1101 50-51 0.8287423243095624 1101 52-53 0.801181285236293 1101 54-55 0.6451228745705855 1101 56-57 0.8241651022930938 1101 58-59 0.7197625574250957 1101 60-61 0.7644584357338005 1101 62-63 0.41819450022573434 1101 64-65 0.4522486361069902 1101 66-67 0.26377771784500936 1101 68-69 -6.031924573903062 1101 70-71 -7.0664545122908 1101 72-73 -4.485088945891267 1101 74-75 -2.5757739325544087 1101 76-77 0.13343998171578875 1101 78-79 0.21879053951079896 1101 80-81 0.18829390464443918 1101 82-83 0.07536375437146958 1101 84-85 0.0436930733722356 1101 86-87 -0.23835058266933729 1101 88-89 -0.49742513032929025 1101 90-91 -0.6227583140607287 1101 92-93 -0.6689716024095311 1101 94-95 -0.8224090009736855 1101 96-97 -0.8299767322115947 1101 98-99 -0.9016156866073786 1101 100-101 -0.7444093595493868 1102 1 0.3823106695631644 1102 2 0.39230960274998594 1102 3 0.3596045307221871 1102 4 0.4789780147548015 1102 5 0.5772632436392513 1102 6 0.43424038121888486 1102 7 0.5769316056093885 1102 8 0.8072634509805283 1102 9 0.8861316342106136 1102 10-11 0.6766568244352129 1102 12-13 0.6768988246533851 1102 14-15 0.8786679128931425 1102 16-17 0.8824604858138798 1102 18-19 0.6767908998124028 1102 20-21 0.6671129363794037 1102 22-23 0.6219774734484886 1102 24-25 0.6189207269591677 1102 26-27 0.6311484991311076 1102 28-29 0.6627710189556595 1102 30-31 0.576885665372032 1102 32-33 0.8211455594722352 1102 34-35 0.7594273420949946 1102 36-37 1.002899749881749 1102 38-39 1.0975127052426714 1102 40-41 1.017280862386876 1102 42-43 0.7482517038042076 1102 44-45 0.8257617345467168 1102 46-47 0.9350123672870403 1102 48-49 1.0187322047016742 1102 50-51 1.0757261868349168 1102 52-53 0.760517758359196 1102 54-55 0.5191297632579968 1102 56-57 0.8253679353475789 1102 58-59 0.7095630121532395 1102 60-61 0.7007889011174449 1102 62-63 0.6981154836162382 1102 64-65 0.4593759623241951 1102 66-67 0.41944032171277 1102 68-69 -6.142342194735498 1102 70-71 -6.547627166445043 1102 72-73 -1.1385687495122703 1102 74-75 0.5333868182844768 1102 76-77 0.9824794678215767 1102 78-79 0.7379675189568999 1102 80-81 0.5524625653228377 1102 82-83 0.38874002905904703 1102 84-85 0.14105011207786688 1102 86-87 0.489538670230548 1102 88-89 0.3950726147238477 1102 90-91 0.4862277660237666 1102 92-93 0.5481797804687609 1102 94-95 0.6608626231488692 1102 96-97 0.41145125670295 1102 98-99 0.20711247662740817 1102 100-101 0.10796026246076451 1103 1 0.3903942909525817 1103 2 0.28432277276539963 1103 3 0.3207671220412891 1103 4 0.36784346900176956 1103 5 0.3900870217190189 1103 6 0.4815114108331784 1103 7 0.31146892217314814 1103 8 0.5166771846434131 1103 9 0.5601412609081677 1103 10-11 0.7100521392295889 1103 12-13 0.6885200635833613 1103 14-15 0.8456682001891807 1103 16-17 0.7319438659658246 1103 18-19 0.5697235311705313 1103 20-21 0.668939603380494 1103 22-23 0.7446454732295891 1103 24-25 0.6785708528221903 1103 26-27 0.7038381031185921 1103 28-29 0.6899130845686017 1103 30-31 0.8260448684219313 1103 32-33 1.0023977255931982 1103 34-35 0.9111085369729182 1103 36-37 1.2094191469248798 1103 38-39 1.136009804920711 1103 40-41 1.2175167985615545 1103 42-43 0.9381424173016555 1103 44-45 0.8754799108096734 1103 46-47 1.0167834333833703 1103 48-49 1.115751052234117 1103 50-51 0.9588462902614907 1103 52-53 1.1033118149793175 1103 54-55 0.8694633458852472 1103 56-57 1.3412678331978753 1103 58-59 1.3164756171531025 1103 60-61 1.2917551506728238 1103 62-63 0.7530810878955805 1103 64-65 0.5608276623871227 1103 66-67 0.5676178159440468 1103 68-69 -6.259351488534463 1103 70-71 -6.828705586864039 1103 72-73 -0.46761534847435726 1103 74-75 1.0035054647096935 1103 76-77 1.169092498293196 1103 78-79 0.9590697553057801 1103 80-81 0.6684315510310022 1103 82-83 0.4296939659268837 1103 84-85 0.13400508868376093 1103 86-87 0.31668506808540187 1103 88-89 0.26779904276759225 1103 90-91 -0.1634816887521886 1103 92-93 -0.08008080160213993 1103 94-95 -0.11826856853687318 1103 96-97 0.13637983171457435 1103 98-99 0.19645516152293396 1103 100-101 0.0622211933911494 1104 1 0.2692937872189738 1104 2 0.3484582714990587 1104 3 0.27718224900271693 1104 4 0.1753096375422487 1104 5 0.43238744414497177 1104 6 0.19187276259624753 1104 7 0.45913635051493884 1104 8 0.5094611305878445 1104 9 0.6676279900041635 1104 10-11 0.5020742348174849 1104 12-13 0.3115717757168994 1104 14-15 0.6089408565194887 1104 16-17 0.4213010331885556 1104 18-19 0.4438266353471292 1104 20-21 0.5425777586313814 1104 22-23 0.5579807457971455 1104 24-25 0.554770607964663 1104 26-27 0.5206695462826829 1104 28-29 0.618166675656532 1104 30-31 0.5703223751742073 1104 32-33 0.4956816668647761 1104 34-35 0.43258292739917437 1104 36-37 0.9094239950104139 1104 38-39 0.8553774977460122 1104 40-41 0.8853224732748686 1104 42-43 0.9625440584078078 1104 44-45 1.2148365523363935 1104 46-47 1.3354892749175669 1104 48-49 1.2781216141914804 1104 50-51 1.0788404959231208 1104 52-53 0.7509377717460772 1104 54-55 0.6530915362759941 1104 56-57 0.6486285704807031 1104 58-59 1.0320288012354943 1104 60-61 1.259742307334907 1104 62-63 0.7961650894469692 1104 64-65 0.8890018733758076 1104 66-67 0.5236906563847903 1104 68-69 -5.469195905876358 1104 70-71 -3.74011470920869 1104 72-73 0.44708404685839653 1104 74-75 1.1663073894580585 1104 76-77 1.25298277735601 1104 78-79 1.0224619782756577 1104 80-81 0.6326258108977321 1104 82-83 0.8686457631063469 1104 84-85 1.197233177673585 1104 86-87 0.729191726901675 1104 88-89 0.9646704505114414 1104 90-91 0.9930048744575011 1104 92-93 1.0269792375563718 1104 94-95 1.1560749539535742 1104 96-97 1.2626616538470792 1104 98-99 1.0863005251176645 1104 100-101 1.2211677219282642 1105 1 0.1166164975261772 1105 2 0.12822068895527394 1105 3 0.1493542271824495 1105 4 0.13670426860742424 1105 5 0.3083959138549943 1105 6 0.2323479276838185 1105 7 0.10088556096570045 1105 8 0.321455101302746 1105 9 0.4914400772852403 1105 10-11 0.35953368069746716 1105 12-13 0.4272712445655955 1105 14-15 0.2093966417621047 1105 16-17 0.16954428613142625 1105 18-19 0.48304031608213194 1105 20-21 0.3900027514536575 1105 22-23 0.43971703635126147 1105 24-25 0.5953677942982907 1105 26-27 0.6733270972444956 1105 28-29 0.6727352946631342 1105 30-31 0.7582027943530747 1105 32-33 0.5805582100746491 1105 34-35 0.6782637341749407 1105 36-37 1.0100825508530775 1105 38-39 0.7179420972292192 1105 40-41 0.7119589935160491 1105 42-43 0.5081225826685056 1105 44-45 0.8579602962353334 1105 46-47 0.8994912129020527 1105 48-49 0.8039649963333133 1105 50-51 0.12919938194092495 1105 52-53 0.19712640223703204 1105 54-55 0.45579035912985333 1105 56-57 0.7579972899753926 1105 58-59 0.6494078566474961 1105 60-61 0.6447893214032412 1105 62-63 0.155110323440649 1105 64-65 0.26122230116794043 1105 66-67 0.33015060470520297 1105 68-69 -3.4889238702748635 1105 70-71 -3.264745056610355 1105 72-73 0.28878660212684437 1105 74-75 0.9907529105605555 1105 76-77 0.8817708192738962 1105 78-79 0.5721192421286574 1105 80-81 0.40212049938466876 1105 82-83 0.4062157458798197 1105 84-85 0.5194392500250054 1105 86-87 0.3628049913288933 1105 88-89 0.3369465056363339 1105 90-91 0.49092154112416964 1105 92-93 0.4037252180329709 1105 94-95 0.546817489124404 1105 96-97 0.6278371491097836 1105 98-99 0.47516608641539904 1105 100-101 0.41847966565493877 1106 1 0.0811903286352873 1106 2 0.32786781623346073 1106 3 0.20128845771344572 1106 4 0.23857937418769382 1106 5 0.2326164968457789 1106 6 0.21166545682397242 1106 7 0.11582446518328027 1106 8 0.27054758023361813 1106 9 0.34948200907569316 1106 10-11 0.4596331607732935 1106 12-13 0.022949492214671352 1106 14-15 0.16712136349944018 1106 16-17 0.3262413599156062 1106 18-19 0.1773324121085622 1106 20-21 0.3692067231487641 1106 22-23 0.2806831192763539 1106 24-25 0.47508418223304005 1106 26-27 0.5802484704449355 1106 28-29 0.6294567692206456 1106 30-31 0.515822405066892 1106 32-33 0.7514621648769051 1106 34-35 0.4776340065251148 1106 36-37 0.6031855784259506 1106 38-39 0.48429530780789065 1106 40-41 0.5406336561284277 1106 42-43 0.49508224631323117 1106 44-45 0.41619368024718995 1106 46-47 0.4753954492517032 1106 48-49 0.7680010719381727 1106 50-51 0.6681448931371214 1106 52-53 0.3980658320097312 1106 54-55 0.271795389443124 1106 56-57 0.3873805135052457 1106 58-59 0.8742243816019801 1106 60-61 0.8654736692456488 1106 62-63 0.4032061246306604 1106 64-65 0.18566155262729467 1106 66-67 0.8472715011520755 1106 68-69 -2.686521332594438 1106 70-71 -3.0682784762848883 1106 72-73 0.8793397488881105 1106 74-75 1.5828367366018128 1106 76-77 1.6291308432992722 1106 78-79 1.3469573523826135 1106 80-81 1.1089433310005639 1106 82-83 0.6770962384000434 1106 84-85 0.5302722548863912 1106 86-87 0.7906772438956686 1106 88-89 0.512283893252004 1106 90-91 0.8055300964106884 1106 92-93 0.4626305618023281 1106 94-95 0.7388431287062112 1106 96-97 0.8843127389515537 1106 98-99 0.7473557742731955 1106 100-101 0.5749271007582628 1107 1 0.028413466210444938 1107 2 0.11923420733909751 1107 3 0.14270905363208541 1107 4 -0.20599560754504864 1107 5 0.132550113858926 1107 6 0.2979681154864693 1107 7 0.02433533718828329 1107 8 0.13025024176157274 1107 9 0.2175135863641131 1107 10-11 0.08919221536494604 1107 12-13 0.15455734546528532 1107 14-15 0.15352240270360795 1107 16-17 0.07703771697011064 1107 18-19 0.06198361488231541 1107 20-21 0.0531640088009695 1107 22-23 0.4021902518656262 1107 24-25 0.23909743972739506 1107 26-27 0.7618489212761617 1107 28-29 0.6268969648355451 1107 30-31 0.485133406972416 1107 32-33 0.6272615067779483 1107 34-35 0.5762700510542444 1107 36-37 0.847575260886039 1107 38-39 0.9221627424158783 1107 40-41 0.8571181277628313 1107 42-43 0.7508621031361145 1107 44-45 0.893358410345428 1107 46-47 1.1346668601528371 1107 48-49 0.9406589225621254 1107 50-51 1.0109079536316052 1107 52-53 0.6447693675007464 1107 54-55 0.8161525967729517 1107 56-57 1.1756331127096047 1107 58-59 1.1016744630285942 1107 60-61 0.963574829276407 1107 62-63 0.6342810036846771 1107 64-65 0.5650474861201396 1107 66-67 0.6058499500825434 1107 68-69 -2.543265414240736 1107 70-71 -2.9462101925477633 1107 72-73 0.8143679974756814 1107 74-75 1.254842696743367 1107 76-77 1.0317842524715886 1107 78-79 0.616367212405251 1107 80-81 0.6759113477730132 1107 82-83 0.5325054966776186 1107 84-85 0.3957368641580423 1107 86-87 0.5752477364192252 1107 88-89 0.5063977758926157 1107 90-91 0.48156679330788066 1107 92-93 0.5854128208296991 1107 94-95 1.0342856262347588 1107 96-97 1.1561009246901968 1107 98-99 0.8704556209714802 1107 100-101 0.6453241092256796 1108 1 -0.07779007825569906 1108 2 0.1150557597919466 1108 3 0.14296556105039926 1108 4 0.12690074013909936 1108 5 0.05550898522628245 1108 6 -0.03413830594355716 1108 7 0.026734264520897 1108 8 0.15230915102207376 1108 9 0.21699474181344414 1108 10-11 0.3691267039532846 1108 12-13 0.26951930658109546 1108 14-15 0.05735507533601236 1108 16-17 0.22127176541503957 1108 18-19 0.22078757617301648 1108 20-21 0.3114341868987296 1108 22-23 0.4022070122935233 1108 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7 -3.284834144822817 2311 8 -0.8300879568755875 2311 9 -3.3407360701999096 2311 10-11 -1.5214322504404905 2311 12-13 -1.254396155042933 2311 14-15 -2.1944663929620987 2311 16-17 -0.03238507551932912 2311 18-19 0.41768690876478587 2311 20-21 -0.532364478284812 2311 22-23 0.05818587769952899 2311 24-25 0.30329434490145246 2311 26-27 0.5324440316505061 2311 28-29 0.8023948647280648 2311 30-31 0.7197845691071052 2311 32-33 1.0485782323215957 2311 34-35 0.5320474387217686 2311 36-37 0.8508897354078329 2311 38-39 0.5224535699249842 2311 40-41 0.8759344701438181 2311 42-43 0.707933311212706 2311 44-45 0.8915074761337536 2311 46-47 1.0116688262709168 2311 48-49 0.8803966042352904 2311 50-51 0.5607270914545062 2311 52-53 1.2044200359902995 2311 54-55 1.0502268992993784 2311 56-57 1.264127701888718 2311 58-59 1.1407302562129047 2311 60-61 1.4941930516461461 2311 62-63 1.441163733344851 2311 64-65 1.20458357252026 2311 66-67 1.400930799753965 2311 68-69 3.7204069106962407 2311 70-71 5.133737753747408 2311 72-73 4.0930109340718985 2311 74-75 2.9702163108933988 2311 76-77 2.607027995636951 2311 78-79 1.9844520667141161 2311 80-81 2.024044946013568 2311 82-83 2.1792158924681893 2311 84-85 1.9220006662411286 2311 86-87 1.9503376503557206 2311 88-89 2.133976366549838 2311 90-91 2.0031202804641772 2311 92-93 1.896912307908888 2311 94-95 2.2161369741680765 2311 96-97 2.1965375138561427 2311 98-99 2.304824794195792 2311 100-101 2.5909982263295888 2312 1 -0.06693631838864178 2312 2 -0.04022051773194235 2312 3 -0.10364848132600457 2312 4 -0.07192559970302881 2312 5 -1.493369286481233 2312 6 -0.3026572091425592 2312 7 0.036871364645534754 2312 8 0.26328754736606896 2312 9 -2.4122117392833573 2312 10-11 -0.17379498279370864 2312 12-13 -1.102167440406852 2312 14-15 -4.026000761805655 2312 16-17 -1.2337292732222664 2312 18-19 -0.29414283512735295 2312 20-21 -0.23732983470492997 2312 22-23 0.15917626862921708 2312 24-25 0.26733940165795644 2312 26-27 0.20170572804676823 2312 28-29 0.33671680208818344 2312 30-31 0.12824108705877535 2312 32-33 0.38778712573729734 2312 34-35 0.3736592698058345 2312 36-37 0.26140878602632966 2312 38-39 0.45579583777279 2312 40-41 0.050036886483255216 2312 42-43 -0.23563383836445695 2312 44-45 0.24383402370334295 2312 46-47 0.36181959620270376 2312 48-49 0.440870758918539 2312 50-51 0.4526840181602161 2312 52-53 0.5306231800459393 2312 54-55 0.6381056668724518 2312 56-57 0.41166311607047135 2312 58-59 0.6411142169725181 2312 60-61 0.32985516617768695 2312 62-63 0.5341847607246706 2312 64-65 0.6656259623241958 2312 66-67 1.02335598436337 2312 68-69 3.6962120221319736 2312 70-71 4.471875845603147 2312 72-73 2.577621009523874 2312 74-75 2.0095163363567643 2312 76-77 2.249497540110731 2312 78-79 1.3983590852219585 2312 80-81 1.728215577371035 2312 82-83 1.5768424386976 2312 84-85 1.315899509668231 2312 86-87 1.025231441314883 2312 88-89 1.0455997231575864 2312 90-91 0.8375078865056942 2312 92-93 1.235830382878401 2312 94-95 0.985787321944052 2312 96-97 1.0427012567029479 2312 98-99 1.3245823561454806 2312 100-101 1.2191801419788355 2313 1 -0.2103919591334389 2313 2 -0.15387057249645153 2313 3 -0.21138396873016774 2313 4 0.049706382772328084 2313 5 0.09018766861186833 2313 6 0.1666062081630244 2313 7 -3.1802014721562166 2313 8 -3.7192425731158565 2313 9 -1.24969804815521 2313 10-11 -0.12689602331944627 2313 12-13 -0.045821602509811044 2313 14-15 -0.554772667610699 2313 16-17 -0.11274077376991443 2313 18-19 0.1604231561103191 2313 20-21 -2.45682600118905 2313 22-23 -0.47763232939926326 2313 24-25 0.09001190987263641 2313 26-27 -1.019453910507444 2313 28-29 -1.6233667137935228 2313 30-31 -0.8073010816159183 2313 32-33 -1.0120554262889883 2313 34-35 -1.7692829536333718 2313 36-37 -2.539741268738183 2313 38-39 -2.280102300233782 2313 40-41 -1.2557339240315244 2313 42-43 -0.17036138491430108 2313 44-45 -0.14938202229885178 2313 46-47 -0.15049557356728727 2313 48-49 -1.7694934250902215 2313 50-51 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0.727621009523876 2314 74-75 0.7312030833447167 2314 76-77 0.5699794678215753 2314 78-79 -2.1498336858623794 2314 80-81 -1.3784109286530644 2314 82-83 -0.027374428772279913 2314 84-85 -0.03169085177755093 2314 86-87 -0.1657324141068024 2314 88-89 -0.001990638288198454 2314 90-91 0.3730500551803928 2314 92-93 0.018962912998883752 2314 94-95 0.3303656351970652 2314 96-97 0.5529422205583678 2314 98-99 0.483618500723793 2314 100-101 0.5944813467981156 2315 1 -0.3737860523498675 2315 2 -0.3182279790492899 2315 3 -0.48471691750727786 2315 4 -0.12648189595402215 2315 5 -0.0714470798883653 2315 6 -0.08346878030028648 2315 7 -1.0960471112886552 2315 8 -2.5743187152478377 2315 9 -0.7603464099457042 2315 10-11 -1.126875834117783 2315 12-13 -0.7298461756524048 2315 14-15 -0.614898994329657 2315 16-17 -0.41060071839382317 2315 18-19 -0.26736006954727287 2315 20-21 -0.8280765780233779 2315 22-23 -0.528858102357276 2315 24-25 -5.108903641580987 2315 26-27 -0.8356023876648067 2315 28-29 -0.7337987627090783 2315 30-31 -1.3876310308545001 2315 32-33 -0.25827427723499596 2315 34-35 -2.353469040389257 2315 36-37 -3.5879184722453346 2315 38-39 -0.8866205281987121 2315 40-41 0.10644016457023042 2315 42-43 -1.6908995713471562 2315 44-45 -5.1456008732448595 2315 46-47 -3.376704041495298 2315 48-49 -2.815219082681363 2315 50-51 -1.2091914023033645 2315 52-53 -0.013374128987145184 2315 54-55 -1.034177373920162 2315 56-57 -0.8419574575947486 2315 58-59 -0.2554410395867137 2315 60-61 -1.2387751928217625 2315 62-63 -0.24085040532022006 2315 64-65 0.35949483884551015 2315 66-67 0.6563753715568126 2315 68-69 3.296216609007395 2315 70-71 3.4560021987313974 2315 72-73 -1.8493375862806651 2315 74-75 -1.3938366695755917 2315 76-77 -0.2507440353280792 2315 78-79 0.07880676426299615 2315 80-81 0.05188538349909777 2315 82-83 -0.906024052648494 2315 84-85 -0.39538879074153144 2315 86-87 -0.005274338148144153 2315 88-89 0.4454116612653465 2315 90-91 0.18922949374683995 2315 92-93 -0.933854957467755 2315 94-95 -0.04942459170037239 2315 96-97 0.19345809114732404 2315 98-99 -2.2002097055021252 2315 100-101 -3.681018622622716 2316 1 -0.4842744491924016 2316 2 -0.406796323216323 2316 3 -0.8121912496845063 2316 4 -0.5841137186948302 2316 5 -0.11456174863754853 2316 6 -0.49294233153257494 2316 7 -0.5078941226429876 2316 8 -1.2245970225879574 2316 9 -5.6616238674339705 2316 10-11 -1.7721440351115803 2316 12-13 -0.797599848773352 2316 14-15 -0.48905297064100495 2316 16-17 -0.5792034777326194 2316 18-19 -0.7318356879867594 2316 20-21 -3.885319076740945 2316 22-23 -1.3090622964909997 2316 24-25 -0.6822971536129003 2316 26-27 -0.3934640915029135 2316 28-29 0.12007648516438252 2316 30-31 0.08930525322571015 2316 32-33 -1.020120522957022 2316 34-35 -0.4951253710199026 2316 36-37 -0.47952599381702754 2316 38-39 -5.5077760970252285 2316 40-41 -5.337004491699151 2316 42-43 -3.7821629412011504 2316 44-45 -2.667628114442003 2316 46-47 -3.091594058415769 2316 48-49 -3.622474022923761 2316 50-51 -1.537158610272062 2316 52-53 -1.3021668034173253 2316 54-55 -0.4724042460825366 2316 56-57 -4.106973421028151 2316 58-59 -1.5996415663417345 2316 60-61 -0.8311726555163297 2316 62-63 -0.2092623308088868 2316 64-65 -0.40912916678954403 2316 66-67 -0.16949687503803545 2316 68-69 2.325860934523618 2316 70-71 3.2563903743061964 2316 72-73 2.09491098390102 2316 74-75 0.8788461442762276 2316 76-77 -1.4345608654572395 2316 78-79 0.11085613223353619 2316 80-81 0.013288902345134801 2316 82-83 0.36010399169692775 2316 84-85 0.3761704577138083 2316 86-87 0.6203640077798376 2316 88-89 0.37592191690996657 2316 90-91 0.15547659391451774 2316 92-93 0.41083174579613413 2316 94-95 0.5695068153021019 2316 96-97 0.378135300570726 2316 98-99 0.40931495168601373 2316 100-101 0.5061133953543298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 27.0 4 86.0 5 283.0 6 612.0 7 1295.0 8 2240.0 9 3722.0 10 5640.0 11 7646.0 12 9440.0 13 11973.0 14 14371.0 15 17962.0 16 21278.0 17 22703.0 18 19768.0 19 16614.0 20 15875.0 21 16141.0 22 16889.0 23 18837.0 24 21634.0 25 25912.0 26 31657.0 27 38644.0 28 45519.0 29 53190.0 30 60807.0 31 69227.0 32 77445.0 33 87217.0 34 96079.0 35 96999.0 36 81743.0 37 54354.0 38 16537.0 39 605.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.16247076066681 22.294621443164658 16.6839080034347 18.85899979273384 2 16.31401938334143 24.200124904586772 38.03881294381606 21.44704276825573 3 19.714331968824442 28.75394898124378 31.409977104003328 20.121741945928445 4 12.815492457064142 19.706058788242352 36.552411739874245 30.926037014819258 5 11.814394002468136 40.84995254297497 32.79761475256666 14.538038701990235 6 28.133412520883866 38.847506609755236 17.15196794014309 15.8671129292178 7 25.148707929301057 37.14303158806192 20.310404097030652 17.397856385606367 8 26.52172384636875 34.78692443324471 19.318802047775254 19.372549672611285 9 26.781303597298 17.404332003056492 20.84677023954234 34.96759416010317 10-11 24.35880177983535 29.29396379730897 25.54995367228659 20.797280750569094 12-13 26.428474955086966 27.247918088989785 27.390474181713227 18.93313277421002 14-15 22.97290046605944 27.412722553095435 28.3666735123194 21.247703468525724 16-17 19.333673459476035 30.957800460049135 28.776799388328 20.93172669214683 18-19 19.018961096749095 27.75920660624907 34.254722466214574 18.96710983078726 20-21 20.71707287289843 26.396836938402075 34.45417020512906 18.43191998357043 22-23 19.961312739598288 26.80785697369784 34.299420153638934 18.931410133064933 24-25 19.500712342960757 27.107610043110625 34.755953151886324 18.635724462042297 26-27 19.527833169816958 27.092882388939536 34.78828214368811 18.591002297555395 28-29 19.039762285705827 27.204807941818466 35.030017257783925 18.72541251469178 30-31 19.039081793174965 27.56530784896804 34.814355353701025 18.581255004155963 32-33 19.06417600817782 27.584513019526398 34.30281528901059 19.04849568328519 34-35 19.339407291803024 27.369401181456926 34.46762511060836 18.823566416131683 36-37 19.622869824169793 27.223893613997536 34.36130485522465 18.79193170660802 38-39 19.494439763331144 27.329466013574198 34.463143177251645 18.712951045843017 40-41 19.30757163574791 27.463467539122334 34.4308925589919 18.79806826613786 42-43 19.190085345536705 27.767272437747565 33.83922524750298 19.203416969212743 44-45 19.34478772861073 28.08005640017461 33.231710563043535 19.343445308171127 46-47 19.596751757967734 28.249873744796457 32.48265177125288 19.670722725982927 48-49 19.851185654574827 28.38549164279003 32.019292634268496 19.74403006836665 50-51 19.9568603399794 28.790538898596342 31.636830716178533 19.61577004524573 52-53 19.954117188606403 29.061119385094425 31.39487634225967 19.5898870840395 54-55 19.50861715508473 29.001926029722497 31.49308489616932 19.996371919023453 56-57 19.927193753172205 29.429528633221874 30.766283897169966 19.876993716435955 58-59 20.056203765948418 28.855368759718726 31.000022670271086 20.08840480406177 60-61 20.08080916491028 28.815859839491942 31.309341628285242 19.793989367312538 62-63 20.32647057463077 28.762113131443957 31.20333408883811 19.70808220508717 64-65 20.087233426435365 28.79956665460874 31.26003221410445 19.85316770485145 66-67 19.907748051635142 29.02864397512765 31.237130598363468 19.82647737487374 68-69 21.061849134354517 28.18480155881448 31.33725212332535 19.416097183505656 70-71 20.928945775931627 28.191864895556286 31.46628365657592 19.41290567193617 72-73 20.614043844355557 28.54349728729591 30.776388215001244 20.066070653347293 74-75 19.929317762753534 29.297444850082567 30.552526640057465 20.220710747106434 76-77 19.556495817262075 29.171300118341332 31.120849535928624 20.151354528467973 78-79 19.743222500714737 29.14345510210313 30.731648869977302 20.381673527204832 80-81 19.420161276520357 29.600111384690248 30.660439978777703 20.319287360011693 82-83 19.56668146832445 29.60548956483126 30.253524644760216 20.57430432208407 84-85 19.457490769923876 29.33327906144164 30.18574863294964 21.023481535684848 86-87 19.59582129732205 29.261649972362463 30.226118973225123 20.916409757090356 88-89 19.75402693131737 28.964117479453677 30.271320626251192 21.010534962977765 90-91 19.395253355437713 29.238518241665524 30.443080600814852 20.923147802081907 92-93 19.369775050500426 29.47397684312096 30.237253702015344 20.91899440436327 94-95 19.19733937135056 29.195899617192055 30.296084748281437 21.31067626317595 96-97 19.23289960838427 29.092333484648876 30.055524012203342 21.61924289476351 98-99 19.316711971858354 28.941690460373533 29.86464881228847 21.876948755479646 100-101 19.270224471942974 28.849707931576376 30.27981852759766 21.600249068883 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10674.0 1 7244.0 2 3716.5 3 3691.0 4 3414.5 5 3136.5 6 4357.0 7 7489.0 8 10327.0 9 11011.5 10 9209.0 11 6670.0 12 5344.0 13 4671.0 14 4229.0 15 4014.5 16 3940.0 17 3827.5 18 3958.0 19 4175.5 20 4345.0 21 4788.5 22 5342.5 23 5880.5 24 6507.0 25 7417.0 26 8740.0 27 10361.0 28 11755.0 29 13115.0 30 14756.0 31 16671.0 32 18975.0 33 21281.0 34 23614.5 35 25626.5 36 27711.5 37 29507.5 38 29979.0 39 30024.0 40 30067.5 41 30544.0 42 31247.0 43 32098.0 44 33019.0 45 33623.0 46 34470.0 47 36103.5 48 41226.0 49 41661.5 50 35886.5 51 31821.5 52 28976.0 53 27109.5 54 25732.0 55 24039.0 56 21533.0 57 19353.0 58 17736.5 59 16140.0 60 14149.0 61 11715.0 62 9221.5 63 6900.5 64 4998.5 65 3726.0 66 2868.0 67 2148.5 68 1527.5 69 1072.0 70 795.5 71 676.0 72 617.5 73 556.0 74 446.0 75 335.0 76 244.0 77 157.5 78 111.0 79 66.0 80 35.5 81 25.5 82 16.5 83 13.0 84 6.5 85 2.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022387134613285377 2 0.014153849569556457 3 2.775264621481658E-4 4 0.07049172138563412 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008464557095519059 12-13 0.0 14-15 0.0 16-17 6.013073346543594E-4 18-19 1.387632310740829E-4 20-21 0.0 22-23 1.8501764143211057E-4 24-25 0.0035153351872101004 26-27 0.00430166016329657 28-29 0.001526395541814912 30-31 1.387632310740829E-4 32-33 5.08798513938304E-4 34-35 0.0022664661075433544 36-37 0.08080645489547428 38-39 0.09139871486746261 40-41 0.0747933815489307 42-43 0.0776611549911284 44-45 0.0776611549911284 46-47 0.07627352268038758 48-49 0.07150931841351073 50-51 0.07081550225814032 52-53 0.05707794238180611 54-55 0.04782706031020058 56-57 0.027891409445890664 58-59 0.024746109541544787 60-61 0.014801411314568845 62-63 0.0021277028764692717 64-65 0.007863249760864699 66-67 0.17280647709759125 68-69 0.7741138117519505 70-71 0.9400746361165536 72-73 0.37933241934618467 74-75 0.060130733465435925 76-77 0.21448170083017415 78-79 0.01470890249385279 80-81 0.003654098418284184 82-83 0.001665158772888995 84-85 9.250882071605529E-5 86-87 0.0018964308246791333 88-89 5.550529242963316E-4 90-91 0.004255405752938543 92-93 0.00430166016329657 94-95 0.08626447531772155 96-97 0.042045259015447124 98-99 0.014801411314568845 100-101 0.01410759515919843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1080978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.57375805198228 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8175974761845 41.68981139375655 2 12.327378992509946 13.208480391196337 3 4.233057985517964 6.803424730084527 4 1.9573591123931902 4.194523340327822 5 0.9939855204934008 2.662576989104357 6 0.5995989026287092 1.9273659922598725 7 0.39972295168388255 1.4990262490935569 8 0.26761636359917673 1.1469771451370891 9 0.18982623657483663 0.9152734383160782 >10 1.078852713806547 10.550450317131867 >50 0.0865088801466178 3.1521346003084068 >100 0.04359285706790053 4.015537983313189 >500 0.002451003696671322 0.9004078562056017 >1k 0.0017507169261938014 2.2583354543456746 >5k 0.0 0.0 >10k+ 7.002867704775206E-4 5.075674119418993 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16520 1.528245718229233 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14028 1.2977137370048235 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12258 1.1339731243374056 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11310 1.046274762298585 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4153 0.38418913243377756 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4113 0.3804887796051354 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3179 0.2940855410563397 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.2254439960850267 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2166 0.20037410567097574 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2145 0.1984314204359386 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2059 0.19047566185435783 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1614 0.14930923663571322 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1173 0.10851284669993284 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 9.250882071605527E-5 0.0 6 0.0 0.0 0.0 9.250882071605527E-5 0.0 7 0.0 0.0 0.0 9.250882071605527E-5 0.0 8 0.0 0.0 0.0 9.250882071605527E-5 0.0 9 0.0 0.0 0.0 9.250882071605527E-5 0.0 10-11 0.0 0.0 0.0 9.250882071605527E-5 0.0 12-13 0.0 0.0 0.0 9.250882071605527E-5 0.0 14-15 0.0 0.0 0.0 1.8501764143211055E-4 0.0 16-17 0.0 0.0 0.0 3.2378087250619345E-4 0.0 18-19 0.0 0.0 0.0 3.700352828642211E-4 0.0 20-21 0.0 0.0 0.0 6.013073346543593E-4 0.0 22-23 0.0 0.0 0.0 0.001526395541814912 0.0 24-25 0.0 0.0 0.0 0.0025439925696915198 0.0 26-27 0.0 0.0 0.0 0.0035153351872101004 0.0 28-29 0.0 0.0 0.0 0.0055042748326052885 0.0 30-31 0.0 0.0 0.0 0.011609856999864937 0.0 32-33 0.0 0.0 0.0 0.02155455522684088 0.0 34-35 0.0 0.0 0.0 0.033441938688853985 0.0 36-37 0.0 0.0 0.0 0.05074108816275632 0.0 38-39 0.0 0.0 0.0 0.07853998878793092 0.0 40-41 0.0 0.0 0.0 0.11336955978752575 0.0 42-43 0.0 0.0 0.0 0.1488004381217749 0.0 44-45 0.0 0.0 0.0 0.18968933687827133 0.0 46-47 0.0 0.0 0.0 0.24403826904895382 0.0 48-49 0.0 0.0 0.0 0.3049553274904762 0.0 50-51 0.0 0.0 0.0 0.3725792754339126 0.0 52-53 0.0 0.0 0.0 0.43585530880369444 0.0 54-55 0.0 0.0 0.0 0.5006114833049331 0.0 56-57 0.0 0.0 0.0 0.5765612251128145 0.0 58-59 0.0 0.0 0.0 0.6580614961636593 0.0 60-61 0.0 0.0 0.0 0.7503390448279244 0.0 62-63 0.0 0.0 0.0 0.836881046607794 0.0 64-65 0.0 0.0 0.0 0.923885592491244 0.0 66-67 0.0 0.0 0.0 1.0220837056813368 0.0 68-69 0.0 0.0 0.0 1.1293476833016027 0.0 70-71 0.0 0.0 0.0 1.2725975921804145 0.0 72-73 0.0 0.0 0.0 1.4418887340907958 0.0 74-75 0.0 0.0 0.0 1.6180255287341647 0.0 76-77 0.0 0.0 0.0 1.8164106947597451 0.0 78-79 0.0 0.0 0.0 2.0420397084862043 0.0 80-81 0.0 0.0 0.0 2.3051810490130236 0.0 82-83 0.0 0.0 0.0 2.5804410450536457 0.0 84-85 0.0 0.0 0.0 2.8553310058114043 0.0 86-87 0.0 0.0 0.0 3.1387317780750394 0.0 88-89 0.0 0.0 0.0 3.448775090704899 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 120 3.8398866E-9 35.58032 2 GGTATCA 9790 0.0 33.543835 1 ATCACGC 100 1.3133231E-6 33.203686 3 CAACGCG 100 4.9938106E-5 28.465572 4 AACGCGG 105 6.9910835E-5 27.093763 5 GTATCAA 13695 0.0 26.578022 1 GTACATG 9150 0.0 23.287056 1 CATTCCG 205 2.7206625E-8 23.128822 9 GAGTACG 125 2.270043E-4 22.778784 1 TACATGG 9255 0.0 22.605324 2 GTATAGG 330 1.8189894E-12 21.570818 1 AGTACTT 11050 0.0 21.386019 12-13 GAGTACT 10805 0.0 21.366394 12-13 TATCAAC 17495 0.0 20.82552 2 ACATGGG 9430 0.0 20.824614 3 TAAGGTG 390 0.0 20.667679 5 ATCAACG 17800 0.0 20.359241 3 GTACTTT 11595 0.0 20.135504 14-15 TCAACGC 18125 0.0 19.971708 4 CATGGGA 6915 0.0 19.896399 4 >>END_MODULE