FastQCFastQC Report
Fri 27 May 2016
SRR939367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979292
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183530.616018839375261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135390.454436825930456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97180.32618487882355945No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40220.1349985164260502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30330.10180271017409506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115050.042.795211
GTATCAA192650.040.087131
TATCAAC245150.031.552942
ATCAACG253050.030.6185673
TCAACGC258300.030.143514
CAACGCA260150.029.7844335
AACGCAG266450.029.047786
GTACATG152450.027.3781811
TACATGG156850.026.1815952
ACGCAGA294550.025.9782037
CGCAGAG294950.025.8471818
GCAGAGT290200.024.7136179
ACATGGG159750.024.7001173
GAGTACT166450.024.21348412-13
AGTACTT171450.023.15815512-13
GTACTTT180750.022.60388814-15
AGAGTAC252150.022.30798510-11
CATGGGG75500.021.931074
CAGAGTA286800.020.60414910-11
CATGGGA77000.020.5715944
Produced by FastQC (version 0.11.5)