FastQCFastQC Report
Fri 27 May 2016
SRR939367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2979292
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241150.8094204931909997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201090.6749590171087627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153140.5140147390722359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57240.19212618299918235No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.15436553382481474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42900.14399394218492179No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG35010.11751114023063197No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC30760.1032460060980931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125900.044.1522871
GTATCAA206300.037.1442571
TATCAAC271700.028.1788522
ATCAACG277450.027.4550253
TCAACGC281550.027.0552164
CAACGCA285800.026.6860435
AACGCAG290350.026.2347816
ACGCAGA329650.022.9921787
AGTACTT206350.022.95743412-13
CGCAGAG330500.022.8757138
TAAGGTG11950.022.5957875
GAGTACT202250.022.41564412-13
GTAAGGT10800.022.3700524
GTACATG157050.022.0275331
GCAGAGT336400.021.7000069
AGAGTAC297100.021.29422210-11
GTACTTT213300.021.15445914-15
TACATGG160500.021.0763192
GGTAAGG10350.021.0541653
TACTTTT227300.020.78936814-15
Produced by FastQC (version 0.11.5)