##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939367_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2979292 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.822046647324264 31.0 30.0 31.0 28.0 34.0 2 31.086283922488967 31.0 31.0 34.0 28.0 34.0 3 31.05001356026868 31.0 31.0 34.0 27.0 34.0 4 35.023786523778135 35.0 35.0 37.0 32.0 37.0 5 34.629510299762494 35.0 35.0 37.0 31.0 37.0 6 34.597445299084484 35.0 35.0 37.0 30.0 37.0 7 34.45798129219963 35.0 35.0 37.0 30.0 37.0 8 34.47540388790357 35.0 35.0 37.0 30.0 37.0 9 35.7806911843485 37.0 35.0 39.0 30.0 39.0 10-11 35.63054510937498 37.0 35.0 39.0 30.0 39.0 12-13 35.57291866658253 37.0 35.0 39.0 30.0 39.0 14-15 36.3530612306548 38.0 35.0 40.0 30.0 41.0 16-17 36.37720052280878 38.0 35.0 40.0 30.0 41.0 18-19 36.37689810196517 38.0 35.0 40.0 30.0 41.0 20-21 36.28437679153302 38.0 34.0 40.0 30.0 41.0 22-23 36.131432065067806 38.0 34.0 40.0 29.0 41.0 24-25 35.88265634922659 38.0 34.0 40.0 28.0 41.0 26-27 35.459170668736064 38.0 34.0 40.0 27.0 41.0 28-29 35.142374765548325 38.0 33.0 40.0 27.0 41.0 30-31 34.85410980192609 37.0 33.0 40.0 26.0 41.0 32-33 34.57072485677806 37.0 33.0 39.5 25.0 40.5 34-35 34.438460882652656 37.0 33.0 40.0 25.0 41.0 36-37 34.37004781672961 37.0 33.0 40.0 24.5 41.0 38-39 34.29913080020354 37.0 33.0 40.0 24.0 41.0 40-41 34.0241557390145 37.0 33.0 40.0 24.0 41.0 42-43 33.6351844666451 37.0 32.0 40.0 23.0 41.0 44-45 33.34269685549452 37.0 32.0 40.0 22.0 41.0 46-47 32.88456468852331 36.5 31.0 39.5 19.0 41.0 48-49 32.45831895631579 36.0 31.0 39.0 16.5 40.5 50-51 32.12707901743099 36.0 30.5 39.0 14.5 40.0 52-53 31.715968928188307 36.0 30.0 39.0 10.5 40.0 54-55 31.457995389508646 35.0 30.0 39.0 9.0 40.0 56-57 31.03401814927842 35.0 29.0 39.0 8.0 40.0 58-59 30.56944418338317 35.0 28.0 38.0 7.5 40.0 60-61 30.240793785906185 34.0 28.0 38.0 6.5 40.0 62-63 29.84023922462115 34.0 28.0 38.0 2.0 40.0 64-65 29.38348322353096 34.0 27.0 37.5 2.0 40.0 66-67 27.656278404399437 33.0 22.5 36.5 2.0 39.0 68-69 24.057183216683697 28.5 11.0 35.0 2.0 38.0 70-71 23.430733711230722 28.5 10.5 34.0 2.0 37.0 72-73 25.18410045071111 30.0 18.5 34.0 2.0 37.0 74-75 25.768038681673367 31.0 20.0 35.0 2.0 37.0 76-77 25.91647361185141 30.5 23.0 34.0 2.0 36.0 78-79 26.73444731164317 32.0 25.0 35.0 2.0 36.0 80-81 26.592847730266115 32.0 24.5 35.0 2.0 36.0 82-83 26.179001252646604 32.0 23.5 35.0 2.0 36.0 84-85 25.826645223093273 32.0 22.5 34.0 2.0 35.5 86-87 25.35759989286045 31.0 20.5 34.0 2.0 35.0 88-89 24.839991514762566 31.0 19.5 34.0 2.0 35.0 90-91 24.353759550926867 31.0 17.5 34.0 2.0 35.0 92-93 23.753068514264463 31.0 9.5 34.0 2.0 35.0 94-95 22.350905517149712 29.0 2.0 34.0 2.0 35.0 96-97 21.499323161341685 29.0 2.0 34.0 2.0 35.0 98-99 21.0868033411965 29.0 2.0 34.0 2.0 35.0 100-101 19.83979129940939 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3584990395676044 1101 2 0.2050071341117281 1101 3 0.2803850635255678 1101 4 0.20787712740887798 1101 5 0.30652369635553356 1101 6 0.5561094116681318 1101 7 0.5234443686644212 1101 8 0.5124299772944809 1101 9 0.7083129638973205 1101 10-11 0.7432248987693981 1101 12-13 0.7554529700962931 1101 14-15 0.7896379441481685 1101 16-17 0.7178177494631726 1101 18-19 0.7220711114248886 1101 20-21 0.7844631633103347 1101 22-23 0.8028866386753677 1101 24-25 0.7838341455903759 1101 26-27 0.8403272388888254 1101 28-29 0.7252217550837088 1101 30-31 0.7390803119461324 1101 32-33 0.7153330130110902 1101 34-35 0.8070130057250111 1101 36-37 0.6943023613155006 1101 38-39 0.658166941803664 1101 40-41 1.0061223255545926 1101 42-43 1.0029492270632332 1101 44-45 0.7583201160936568 1101 46-47 0.644121525650192 1101 48-49 0.6319581110268544 1101 50-51 0.5667190187639655 1101 52-53 0.6295047626967509 1101 54-55 0.5200916392782169 1101 56-57 0.5349480831433944 1101 58-59 0.5304401907331737 1101 60-61 0.26548472993543726 1101 62-63 0.2302752528292693 1101 64-65 -0.14861541874763162 1101 66-67 -3.330937446882132 1101 68-69 -7.735563858229437 1101 70-71 -10.555552074712118 1101 72-73 -10.640743343836823 1101 74-75 -9.503389499901374 1101 76-77 -4.762706186220338 1101 78-79 -1.8191641887406753 1101 80-81 -1.1993545763377647 1101 82-83 -1.012014076639705 1101 84-85 -1.102088534062748 1101 86-87 -1.1357209327599165 1101 88-89 -0.981295743858599 1101 90-91 -1.0777288271912226 1101 92-93 -1.5982577101631676 1101 94-95 -4.859852943407141 1101 96-97 -2.115854169147571 1101 98-99 -1.465935081030949 1101 100-101 -1.1855124199927793 1102 1 0.49562593567084434 1102 2 0.3244746783163457 1102 3 0.5048315625212538 1102 4 0.2824459255788554 1102 5 0.5092857971266085 1102 6 0.5761906385387476 1102 7 0.5400907148712832 1102 8 0.6800498414192617 1102 9 0.8345109060877931 1102 10-11 0.7274073835487727 1102 12-13 0.6945820200129234 1102 14-15 0.6957418254934069 1102 16-17 0.8305557470978471 1102 18-19 0.8511325445783413 1102 20-21 0.898392157374083 1102 22-23 0.8421388391822049 1102 24-25 0.8546139636992578 1102 26-27 0.7694376329384482 1102 28-29 0.831752930708042 1102 30-31 0.6828156563895789 1102 32-33 0.5569200917080863 1102 34-35 0.705690613414788 1102 36-37 1.11110818220412 1102 38-39 1.087361670840103 1102 40-41 1.047099046588798 1102 42-43 1.1786864905085253 1102 44-45 1.1056194785892757 1102 46-47 1.1538285632928194 1102 48-49 0.9526067214623097 1102 50-51 0.999217958527101 1102 52-53 1.0848875664613296 1102 54-55 0.7956342959324765 1102 56-57 0.7710600556284994 1102 58-59 0.751140504520265 1102 60-61 0.6904515516016225 1102 62-63 0.5881763566586322 1102 64-65 0.4558735685908317 1102 66-67 -2.586374544095186 1102 68-69 -7.270363332753458 1102 70-71 -10.324631319486485 1102 72-73 -8.973348836060975 1102 74-75 -7.656332790941146 1102 76-77 -3.7918561576665404 1102 78-79 -0.9832794905308724 1102 80-81 -0.2738298531913337 1102 82-83 -0.0840033086482137 1102 84-85 -0.02275171715373503 1102 86-87 -0.09531129953555251 1102 88-89 0.030896487213173174 1102 90-91 -0.06459546785534798 1102 92-93 -0.5824406878675426 1102 94-95 -4.206094981772125 1102 96-97 -1.1179519235246538 1102 98-99 -0.22499773126104472 1102 100-101 -0.05586920090213354 1103 1 0.43557272859831997 1103 2 0.35445369722213727 1103 3 0.47772770785918794 1103 4 0.34399219362811806 1103 5 0.43275488772950155 1103 6 0.5147386626143771 1103 7 0.6118179010013023 1103 8 0.5295464530427125 1103 9 0.8439976587020936 1103 10-11 0.7738968219536488 1103 12-13 0.6740857627524335 1103 14-15 0.9103469300743399 1103 16-17 0.8903898894429929 1103 18-19 0.7017631615382882 1103 20-21 0.8638092706491918 1103 22-23 0.9175845558637548 1103 24-25 0.8301350263671239 1103 26-27 0.7881812846663223 1103 28-29 0.9368022520678849 1103 30-31 0.8786970202308737 1103 32-33 0.9466961062474937 1103 34-35 0.9660241531674743 1103 36-37 0.9015727046824864 1103 38-39 0.9942730643110522 1103 40-41 1.14507290618036 1103 42-43 1.0747282386757533 1103 44-45 0.9628819457624616 1103 46-47 0.9274279085984745 1103 48-49 0.8970420388189027 1103 50-51 0.8960338231113276 1103 52-53 0.9763456647952999 1103 54-55 1.0260324104408305 1103 56-57 1.2160414340302488 1103 58-59 1.1758402608518708 1103 60-61 1.1571949822912089 1103 62-63 1.074630876277535 1103 64-65 0.7482634046891903 1103 66-67 -2.5861578914919328 1103 68-69 -7.173341560482534 1103 70-71 -10.74221574821655 1103 72-73 -8.652628821395261 1103 74-75 -7.2052065044018505 1103 76-77 -3.74430038497108 1103 78-79 -0.863296577141039 1103 80-81 -0.16153376539352138 1103 82-83 -0.0071689101793417365 1103 84-85 -0.10686358182217148 1103 86-87 0.005311028488769409 1103 88-89 0.07091388959231537 1103 90-91 0.08027078644042618 1103 92-93 0.25077851544732965 1103 94-95 1.230097186455552 1103 96-97 1.43712283478051 1103 98-99 1.0546114870879393 1103 100-101 0.9514408509015198 1104 1 0.4028596505033555 1104 2 0.23141656586398796 1104 3 0.33481719287564005 1104 4 0.2310656570118681 1104 5 0.4265298012808998 1104 6 0.48573513470682883 1104 7 0.4170122507521441 1104 8 0.26946690458689915 1104 9 0.6389386007950861 1104 10-11 0.6601105218488712 1104 12-13 0.6034425624138038 1104 14-15 0.7491032770721233 1104 16-17 0.7153956614285661 1104 18-19 0.624930731025124 1104 20-21 0.6339649131149088 1104 22-23 0.5985496843254126 1104 24-25 0.7603025713667435 1104 26-27 0.8278403789543276 1104 28-29 0.7689459150399003 1104 30-31 0.7831192794559882 1104 32-33 0.6520777831667246 1104 34-35 0.65274832396927 1104 36-37 0.8553631063951954 1104 38-39 0.7292942370036144 1104 40-41 0.8054292038539046 1104 42-43 0.9650431092346068 1104 44-45 0.800626927195907 1104 46-47 0.6362684041496749 1104 48-49 0.5418518431485992 1104 50-51 0.5680442843979172 1104 52-53 0.8650397443122664 1104 54-55 0.7486116309429214 1104 56-57 0.7917885126184565 1104 58-59 0.5616785630485523 1104 60-61 0.42316672229998886 1104 62-63 0.49298032452818674 1104 64-65 0.4234330660835255 1104 66-67 -2.868338022918298 1104 68-69 -7.116977246745385 1104 70-71 -8.666629279499837 1104 72-73 -8.035810873318646 1104 74-75 -7.047255113265873 1104 76-77 -3.7758566172287864 1104 78-79 -0.9037253132336183 1104 80-81 -0.10919965313365054 1104 82-83 -0.036771237863707285 1104 84-85 0.0918346167936015 1104 86-87 0.19236805045904504 1104 88-89 0.20101549402939511 1104 90-91 0.27417736248109037 1104 92-93 0.43380900902205966 1104 94-95 1.2671027415544032 1104 96-97 1.2317177201016527 1104 98-99 0.8744825094465121 1104 100-101 0.8656324143575027 1105 1 0.4973869637706585 1105 2 0.3064197323474396 1105 3 0.5082624495005774 1105 4 0.41158316974490106 1105 5 0.57075079532774 1105 6 0.5067153922291894 1105 7 0.6316570373397354 1105 8 0.6250990560265279 1105 9 0.8946802692561633 1105 10-11 0.9459385424789986 1105 12-13 0.9036033691780503 1105 14-15 1.2051300619633167 1105 16-17 1.1875210454263296 1105 18-19 1.092739321238092 1105 20-21 1.1623537128620356 1105 22-23 1.1821853664476905 1105 24-25 1.2468941325618559 1105 26-27 1.2851515941542075 1105 28-29 1.2855610561390023 1105 30-31 1.204173637407635 1105 32-33 1.3826806601774138 1105 34-35 1.5593988053390717 1105 36-37 1.499250515590866 1105 38-39 1.4137812379566057 1105 40-41 1.447705029474271 1105 42-43 1.6059301413679137 1105 44-45 1.1884031948376332 1105 46-47 1.161399048926313 1105 48-49 1.1550668970697089 1105 50-51 1.2394303191639757 1105 52-53 1.4972129655782531 1105 54-55 1.3588850067298601 1105 56-57 1.4633491005887684 1105 58-59 1.3952576686655433 1105 60-61 1.4519363576059945 1105 62-63 1.7044059744247981 1105 64-65 1.478379507706478 1105 66-67 2.199575522178815 1105 68-69 -4.473787192822119 1105 70-71 -5.339352912340058 1105 72-73 -1.6061562434053123 1105 74-75 1.1938767821538185 1105 76-77 1.3531208354366804 1105 78-79 1.3418946276283954 1105 80-81 1.4487652597428173 1105 82-83 1.6484066438999285 1105 84-85 1.5819123969467803 1105 86-87 1.6019681851278484 1105 88-89 1.550505782379627 1105 90-91 1.6658097509460532 1105 92-93 1.7631926634158042 1105 94-95 2.3893870157042087 1105 96-97 2.4130604908848667 1105 98-99 2.0890168442890378 1105 100-101 2.072374412887502 1106 1 0.18635729883548535 1106 2 0.18142529876568148 1106 3 0.15688860519140135 1106 4 0.08040494440531631 1106 5 0.29487893301203627 1106 6 0.24589573485562966 1106 7 0.2427784751915354 1106 8 0.13346201664818125 1106 9 0.3649203019252667 1106 10-11 0.4167216658483426 1106 12-13 0.21246398284090873 1106 14-15 0.37108100271196065 1106 16-17 0.31817392288765234 1106 18-19 0.3221326152008359 1106 20-21 0.4306698432397056 1106 22-23 0.3554080755517717 1106 24-25 0.47301106107022406 1106 26-27 0.6647705992960482 1106 28-29 0.6145090104988142 1106 30-31 0.5073456984237694 1106 32-33 0.6414375837777513 1106 34-35 0.6032444033981008 1106 36-37 0.6030026257099976 1106 38-39 0.7744464909491171 1106 40-41 0.8108130577264916 1106 42-43 0.9154015577775638 1106 44-45 0.6845546320890037 1106 46-47 0.7076544812494276 1106 48-49 0.5948021298958892 1106 50-51 0.5669579327378678 1106 52-53 0.7643645271862276 1106 54-55 0.7598102565520186 1106 56-57 0.5346685783118978 1106 58-59 0.758013106260961 1106 60-61 0.7293485600488054 1106 62-63 0.8259991194907563 1106 64-65 0.7178691732534119 1106 66-67 1.4910309154956103 1106 68-69 -5.100667498403016 1106 70-71 -6.211591957999328 1106 72-73 -2.4035501226371743 1106 74-75 0.28940375224540205 1106 76-77 0.5920860594184596 1106 78-79 0.6504003412789423 1106 80-81 0.775147781564268 1106 82-83 0.6416624107745861 1106 84-85 0.6145746577117102 1106 86-87 0.7365106708640958 1106 88-89 1.044697796837415 1106 90-91 0.8726225152855598 1106 92-93 0.9603709708464514 1106 94-95 2.0166016985342736 1106 96-97 2.052188485214831 1106 98-99 1.7665062402078213 1106 100-101 1.5886979833986175 1107 1 0.005884478804041748 1107 2 -0.057084764316325476 1107 3 -0.013804896731127059 1107 4 -0.005049346913459374 1107 5 0.0940918228122527 1107 6 0.1396018636463694 1107 7 0.06826455807060228 1107 8 0.10990706588749788 1107 9 0.25482370734213333 1107 10-11 0.1314001928918671 1107 12-13 0.25354662246483173 1107 14-15 0.26534303456520547 1107 16-17 0.2006221694379562 1107 18-19 0.344697009042342 1107 20-21 0.31536587777933534 1107 22-23 0.2833375568654546 1107 24-25 0.4310002490497524 1107 26-27 0.47449641584553603 1107 28-29 0.4947778280467361 1107 30-31 0.446074047127226 1107 32-33 0.36406159258402226 1107 34-35 0.534352021840462 1107 36-37 0.49868532959877854 1107 38-39 0.33140967352124306 1107 40-41 0.44376818431241816 1107 42-43 0.620814848524553 1107 44-45 0.3793682968837615 1107 46-47 0.44130258085569096 1107 48-49 0.5732014773547078 1107 50-51 0.6460224650109474 1107 52-53 0.5995922751549863 1107 54-55 0.5344593910837041 1107 56-57 0.5633459606665916 1107 58-59 0.5196717175898264 1107 60-61 0.5633838697812443 1107 62-63 0.6292267103635147 1107 64-65 0.5800489801753983 1107 66-67 1.3857105114588002 1107 68-69 -4.906739119540095 1107 70-71 -5.557678410628263 1107 72-73 -2.1306050998429704 1107 74-75 0.5217038459750221 1107 76-77 0.5466748972480637 1107 78-79 0.6315175605465058 1107 80-81 0.46506266351024195 1107 82-83 0.5623984054955677 1107 84-85 0.6376268271971632 1107 86-87 0.6390858224464431 1107 88-89 0.606084954443876 1107 90-91 0.5199280272522273 1107 92-93 0.8088188189138741 1107 94-95 1.5698030527091333 1107 96-97 1.7623683795770404 1107 98-99 1.3829142310955156 1107 100-101 1.2015407474470159 1108 1 0.04958238956203331 1108 2 0.03168887814859289 1108 3 0.10245414301412126 1108 4 0.02826189914201649 1108 5 0.14020512979512745 1108 6 0.29876666352415526 1108 7 0.23382385065309563 1108 8 0.23481485526517076 1108 9 0.2720949615025461 1108 10-11 0.38125734991303517 1108 12-13 0.3337935691432463 1108 14-15 0.18721314594417038 1108 16-17 0.13953146805023664 1108 18-19 0.17230002013658208 1108 20-21 0.3496718979547708 1108 22-23 0.35764831244790685 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1.094759602685631 2312 86-87 1.025476440000336 2312 88-89 1.0430655840516998 2312 90-91 1.2281621258702273 2312 92-93 1.3977863153397436 2312 94-95 2.1353311832280113 2312 96-97 2.2814248788902454 2312 98-99 2.0378040637420547 2312 100-101 1.917218150524974 2313 1 -0.16745623733010007 2313 2 -0.14544941845467108 2313 3 -0.06460825716308705 2313 4 -0.0762682229153242 2313 5 -0.07007439475868438 2313 6 0.1140381144312812 2313 7 -3.526901877359542 2313 8 -1.1794539704565281 2313 9 -0.8911345605995322 2313 10-11 -0.11509054168077881 2313 12-13 -1.4661052022816747 2313 14-15 -0.43140737412114305 2313 16-17 -0.10996848791612734 2313 18-19 0.3488103190066667 2313 20-21 0.19111604024903528 2313 22-23 -1.1368231485101106 2313 24-25 -2.8484910380000272 2313 26-27 -2.1625775242361556 2313 28-29 -0.3289352399175627 2313 30-31 -0.10552023961075463 2313 32-33 -2.539997382037665 2313 34-35 -2.6881325273555845 2313 36-37 -1.0365193366075047 2313 38-39 -0.6266592728132707 2313 40-41 -0.4419758837711072 2313 42-43 -0.5182408458797738 2313 44-45 -0.07120152144237579 2313 46-47 0.030319887901427478 2313 48-49 -0.0552688119887037 2313 50-51 -0.024412968586197792 2313 52-53 0.1792817684603456 2313 54-55 0.5003417399634564 2313 56-57 0.7598465155479381 2313 58-59 0.17873667625021028 2313 60-61 0.6135155901427432 2313 62-63 0.3523510899672111 2313 64-65 0.32583373750281197 2313 66-67 1.6804585388590034 2313 68-69 4.761533010399173 2313 70-71 4.7934724025586455 2313 72-73 3.0780110528325615 2313 74-75 2.244045806508108 2313 76-77 1.332323758271027 2313 78-79 0.9206823756565399 2313 80-81 1.0599510726239352 2313 82-83 0.9302119954465766 2313 84-85 1.0818890236445178 2313 86-87 1.057700368610707 2313 88-89 0.9916726866652006 2313 90-91 0.9637644304029607 2313 92-93 1.1888821497332565 2313 94-95 1.8530042958011634 2313 96-97 1.779766723970397 2313 98-99 1.5149333938282012 2313 100-101 1.4116378371957587 2314 1 -0.286086757306947 2314 2 -0.2396778553885568 2314 3 -0.15952677248463942 2314 4 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-0.8730777252786126 2314 66-67 -0.6966248135335533 2314 68-69 1.3881621254646745 2314 70-71 1.5042196873145492 2314 72-73 -0.536221047059648 2314 74-75 -0.03978691432908832 2314 76-77 -0.2296153960806926 2314 78-79 0.19147338630476796 2314 80-81 0.5921235782291383 2314 82-83 0.27143441181874195 2314 84-85 0.12199796885064629 2314 86-87 0.10949750529663405 2314 88-89 0.16125025716235086 2314 90-91 0.611028270492767 2314 92-93 0.6551188941561712 2314 94-95 0.5374171165858144 2314 96-97 0.6865940334234644 2314 98-99 0.333384837835613 2314 100-101 0.21033450926717379 2315 1 -0.3861584677240337 2315 2 -0.3001293837388985 2315 3 -0.3700848067007527 2315 4 -0.23991887813879487 2315 5 -1.3828681224119848 2315 6 -0.35430263325914524 2315 7 -0.4104770562118816 2315 8 -0.21018393568827776 2315 9 -0.07720627364659549 2315 10-11 -1.9966012444862784 2315 12-13 -1.5046943860307493 2315 14-15 -0.1785882917803292 2315 16-17 -2.025607796497148 2315 18-19 -3.3860643400535935 2315 20-21 -2.3841162290479687 2315 22-23 -1.6282454772164314 2315 24-25 -1.1362318996606149 2315 26-27 -0.5294750696799184 2315 28-29 -0.47911506300145845 2315 30-31 -3.074101206174255 2315 32-33 -2.626823439016448 2315 34-35 -1.7582292130907362 2315 36-37 -5.152332853720345 2315 38-39 -2.707481117303818 2315 40-41 -1.885268068209669 2315 42-43 -1.9812525965600827 2315 44-45 -1.8187674819348203 2315 46-47 -1.689791647536893 2315 48-49 -4.954587509706464 2315 50-51 -4.437702141106165 2315 52-53 -2.9614154434685354 2315 54-55 -4.463051905203148 2315 56-57 -6.777264099523617 2315 58-59 -7.579398599823087 2315 60-61 -6.179643996416331 2315 62-63 -7.091050761890475 2315 64-65 -6.4544439676880785 2315 66-67 -4.360839902394417 2315 68-69 -2.406622089765488 2315 70-71 -0.05311861982505306 2315 72-73 -1.4886392424299117 2315 74-75 -3.531207451768637 2315 76-77 -5.734985905789859 2315 78-79 -4.955534381734992 2315 80-81 -4.699905614622427 2315 82-83 -7.765105048339585 2315 84-85 -5.8483524230838775 2315 86-87 -6.205539427334433 2315 88-89 -4.6522267089571265 2315 90-91 -4.260896571338073 2315 92-93 -4.928737783920578 2315 94-95 -3.0292106922193724 2315 96-97 -1.939147180284177 2315 98-99 -3.134611827453366 2315 100-101 -2.286675908172235 2316 1 -0.3481021912390396 2316 2 -0.3414423062157681 2316 3 -0.9138229846799 2316 4 -0.363186081470225 2316 5 -0.40606201088542093 2316 6 -2.424906307799823 2316 7 -0.4811525793133953 2316 8 -0.5922247280752657 2316 9 -0.09352176214349583 2316 10-11 -0.07403713064296369 2316 12-13 -0.721416038840907 2316 14-15 -1.150961786992866 2316 16-17 -1.5722353130296582 2316 18-19 -0.2818795279595747 2316 20-21 -4.276338988912478 2316 22-23 -4.520496829875558 2316 24-25 -2.9249524087589904 2316 26-27 -2.5103435574876585 2316 28-29 -1.6809442687082807 2316 30-31 -0.580278716108424 2316 32-33 -2.060450764930323 2316 34-35 -2.589322777512507 2316 36-37 -5.016110181833664 2316 38-39 -2.490147285429451 2316 40-41 -2.0575741137528603 2316 42-43 -3.5292716290972166 2316 44-45 -0.9585561885140947 2316 46-47 -0.996270481537394 2316 48-49 -0.4554892824597303 2316 50-51 -0.2964449379213683 2316 52-53 -2.890468655392464 2316 54-55 -1.1478874599766762 2316 56-57 0.022044541365545456 2316 58-59 0.2609143079908094 2316 60-61 -1.2345201676211914 2316 62-63 -0.897926229272695 2316 64-65 0.147732017297038 2316 66-67 1.0209112963035132 2316 68-69 3.6310434185264207 2316 70-71 3.6771794783875293 2316 72-73 1.9066378144184277 2316 74-75 1.0323550638107726 2316 76-77 -0.29904402980927003 2316 78-79 0.11844617516466727 2316 80-81 -0.3238465814987741 2316 82-83 -0.14086862258554333 2316 84-85 0.18649779466125338 2316 86-87 -0.2233871963633014 2316 88-89 0.44807828085210843 2316 90-91 0.5263312472516404 2316 92-93 0.8575692000527972 2316 94-95 1.321387557021815 2316 96-97 1.6708192415108662 2316 98-99 0.6537487056922835 2316 100-101 0.19119986713238646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 55.0 4 124.0 5 297.0 6 809.0 7 2096.0 8 4389.0 9 7063.0 10 11110.0 11 16669.0 12 20738.0 13 26439.0 14 32003.0 15 39829.0 16 47252.0 17 52519.0 18 45829.0 19 37785.0 20 36030.0 21 36492.0 22 37776.0 23 42913.0 24 51522.0 25 63366.0 26 78793.0 27 96152.0 28 116413.0 29 137071.0 30 159365.0 31 182564.0 32 208214.0 33 237251.0 34 268930.0 35 296934.0 36 291393.0 37 216590.0 38 73370.0 39 3142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.60116671165382 20.432460851103258 15.992799369374088 21.97357306786883 2 16.405872591763025 22.098626051476657 37.720118961174464 23.775382395585854 3 19.181436394955583 25.720674576375863 30.89401777335018 24.203871255318376 4 13.598465675737724 18.291661240321528 35.80736631387592 32.30250677006483 5 13.579917893161758 38.0825425126599 33.09014331942957 15.247396274748773 6 31.30841152864506 36.685024495752685 16.618679874278854 15.387884101323401 7 27.556714816808825 34.28233956255379 20.04999845600901 18.110947164628374 8 27.047835526024304 33.987336588692884 19.262126706613518 19.702701178669294 9 26.87943981321737 16.379562661196015 20.048320204934594 36.692677320652024 10-11 25.412446984048557 26.982601906761737 26.177477736321247 21.42747337286845 12-13 26.490823994425522 24.553215998968884 27.424233676994398 21.531726329611196 14-15 23.064691208515313 25.567970511114723 26.735026308263844 24.632311972106123 16-17 21.58041275003902 28.8894315246779 27.186791461709774 22.343364263573307 18-19 21.4072849932777 26.208541949070263 31.17205387979538 21.21211917785666 20-21 22.439617345200585 25.263158248052974 31.29721131638366 21.000013090362774 22-23 22.015381506747243 25.279059588653947 31.33081618048852 21.374742724110295 24-25 21.97688242710013 25.624376529725858 31.26687481455326 21.131866228620762 26-27 21.564313671654336 25.95951842071963 31.256501149102757 21.21966675852328 28-29 21.282120317809987 26.1431011081453 31.449031996860437 21.125746577184273 30-31 21.329586192080992 25.76803528511393 31.409424132977083 21.492954389828007 32-33 21.45363820757844 25.89026507678222 31.044169393353737 21.611927322285602 34-35 22.12691479010928 25.964509404032775 30.68291544065999 21.225660365197953 36-37 22.07146695550756 25.618559012959707 30.86034595394011 21.449628077592617 38-39 21.56135596283948 26.022308204923434 30.956157837630442 21.460177994606642 40-41 21.709725725001885 25.829117816996217 31.0499680864594 21.411188371542497 42-43 21.99575195360074 25.962471110495905 30.326747472724847 21.715029463178503 44-45 21.719903412073492 26.373543307086617 30.15402834645669 21.752524934383203 46-47 22.048385706863336 26.61514564241894 29.48142956899829 21.855039081719436 48-49 22.28772328980395 26.66603280343039 29.17433378006133 21.871910126704332 50-51 22.138464533709705 27.0462264608932 28.83575543770312 21.979553567693973 52-53 22.652670803823302 27.10574873986683 28.425678720245816 21.815901736064053 54-55 21.90841630296608 26.94884458244262 28.81779818270056 22.324940931890733 56-57 22.110277908306728 27.399999294891842 28.500807432780107 21.98891536402132 58-59 22.280270818100824 26.849638078894166 28.63722150941344 22.23286959359157 60-61 22.187527798524684 26.941465748021585 29.064022624852093 21.806983828601638 62-63 21.979313149296186 26.996036399270885 28.927773584867662 22.096876866565264 64-65 21.849339461680657 26.929796677655222 28.762963800882197 22.45790005978193 66-67 21.741851341721492 26.994261370569706 29.158129848626118 22.105757439082684 68-69 21.92573790395932 26.443195038476347 29.72084660650904 21.910220451055295 70-71 21.590560151023492 26.6852657078879 29.820873008869718 21.903301132218893 72-73 22.087645440794734 26.991367678267487 28.59685261668462 22.32413426425316 74-75 21.733412867731037 27.40196990500413 28.25598982927837 22.60862739798646 76-77 21.766187588319703 27.475884808365024 28.2086939065511 22.54923369676417 78-79 21.848009468777672 27.48107285064319 28.175521491050514 22.49539618952862 80-81 21.680431612489937 27.94070581269459 27.906620012811967 22.472242562003515 82-83 21.94081681151092 27.657846226553158 27.765069687697615 22.636267274238307 84-85 21.627302728299206 27.501332531353086 27.751626896591542 23.119737843756166 86-87 21.971429386581008 27.35275410039657 27.782152429190226 22.893664083832192 88-89 21.645771415807015 27.38042312778099 27.64053757545274 23.333267880959248 90-91 21.523181197589615 27.624834581853975 27.822958737980173 23.029025482576234 92-93 21.629805737271454 27.295683108284212 27.89217781657964 23.182333337864694 94-95 21.205140009981548 27.021772527325204 28.239575345136362 23.533512117556878 96-97 21.178241889493563 26.985137397067856 28.218640917158023 23.617979796280565 98-99 21.32259328791921 27.191186627871357 27.569885686998397 23.916334397211035 100-101 21.14532099282412 26.82607283630587 28.0135545315622 24.015051639307806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4922.0 1 3356.5 2 1839.0 3 2011.5 4 1970.0 5 1951.0 6 2988.5 7 5782.5 8 8688.5 9 10005.0 10 9389.5 11 8022.0 12 7577.5 13 7571.0 14 7721.0 15 8013.5 16 8359.5 17 8793.5 18 9170.0 19 9587.0 20 10220.5 21 11253.5 22 12506.0 23 13928.0 24 15579.5 25 17752.0 26 20974.5 27 24747.5 28 28062.5 29 31552.0 30 35480.0 31 39841.0 32 44806.0 33 48401.5 34 51578.0 35 55328.0 36 59672.0 37 64231.0 38 67298.0 39 68500.0 40 71095.5 41 78791.0 42 87036.0 43 92035.5 44 94371.0 45 96809.0 46 98997.5 47 103189.5 48 113599.0 49 117595.5 50 112965.5 51 109427.0 52 107098.5 53 106926.5 54 105179.5 55 96705.0 56 85219.5 57 74890.5 58 67066.5 59 59693.5 60 51694.5 61 43755.5 62 36038.5 63 28427.0 64 21663.0 65 16537.5 66 12789.0 67 10369.5 68 7688.0 69 5380.0 70 4176.5 71 3448.0 72 3026.5 73 2573.0 74 2076.5 75 1641.5 76 1067.0 77 559.0 78 302.5 79 170.0 80 98.0 81 61.0 82 37.0 83 24.0 84 13.5 85 7.0 86 5.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022287174268248968 2 0.016950335851605013 3 0.0 4 0.0 5 1.0069506446498028E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.349551504182873E-4 18-19 0.001023733155393966 20-21 3.356502148832676E-5 22-23 0.0 24-25 0.0 26-27 3.6921523637159433E-4 28-29 0.017823026410301507 30-31 0.004430582836459132 32-33 0.008374472861337526 34-35 0.009314293463010674 36-37 0.0938981476135941 38-39 0.0957777888169404 40-41 0.11017718303543257 42-43 0.09616378656405616 44-45 0.09161572615238789 46-47 0.09305902207638593 48-49 0.09515683591940635 50-51 0.0933946722912692 52-53 0.07137601819492685 54-55 0.06031634361452318 56-57 0.03442092953627909 58-59 0.015641300013560268 60-61 0.008961860737383245 62-63 0.0018964237140904616 64-65 0.004397017814970806 66-67 0.4272827235463996 68-69 0.8241723201351194 70-71 0.9119112863056054 72-73 0.286594935978078 74-75 0.1503209487354714 76-77 0.013845571363934786 78-79 0.007988475114221768 80-81 0.0015272084777188677 82-83 0.0 84-85 0.0 86-87 2.349551504182873E-4 88-89 8.894730694406591E-4 90-91 0.0033397196380885123 92-93 0.0017957286496254816 94-95 0.2284938837817844 96-97 0.2372040068580052 98-99 0.013744876299469808 100-101 0.00719969710924609 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2979292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.68164096681403 #Duplication Level Percentage of deduplicated Percentage of total 1 66.04571021089282 23.566193191433207 2 14.297114253809983 10.20288995331934 3 6.427997943333709 6.880845442483571 4 3.620647423506549 5.167625657319237 5 2.271134791279391 4.051890810483567 6 1.4787187821485737 3.165786760530594 7 1.098151680042522 2.7428697782066633 8 0.8056411809105004 2.2997279492262837 9 0.6082096334337352 1.9531725995466098 >10 3.0074219715110924 18.631893333736485 >50 0.19264018343226752 4.760457504740067 >100 0.1301076896689655 9.032783145522663 >500 0.012666056953174689 3.0896308880026027 >1k 0.003454379169038989 2.2197723751536986 >5k 9.59549769177497E-5 0.19598008846389603 >10k+ 2.878649307532491E-4 2.038480521831489 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24115 0.8094204931909997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20109 0.6749590171087627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15314 0.5140147390722359 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5724 0.19212618299918235 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4599 0.15436553382481474 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4290 0.14399394218492179 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 3501 0.11751114023063197 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 3076 0.1032460060980931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.356502148832676E-5 0.0 14-15 0.0 0.0 0.0 3.356502148832676E-5 0.0 16-17 0.0 0.0 0.0 6.713004297665352E-5 0.0 18-19 0.0 0.0 0.0 1.0069506446498027E-4 0.0 20-21 0.0 0.0 0.0 1.8460761818579716E-4 0.0 22-23 0.0 0.0 0.0 6.880829405106985E-4 0.0 24-25 0.0 0.0 0.0 0.0011412107306031098 3.356502148832676E-5 26-27 0.0 0.0 0.0 0.0018125111603696448 3.356502148832676E-5 28-29 0.0 0.0 0.0 0.003977455046366721 3.356502148832676E-5 30-31 0.0 0.0 0.0 0.009767421253103085 3.356502148832676E-5 32-33 0.0 0.0 0.0 0.018997802162392944 3.356502148832676E-5 34-35 0.0 0.0 0.0 0.031819640370933766 3.356502148832676E-5 36-37 0.0 0.0 0.0 0.05328447161271872 3.356502148832676E-5 38-39 0.0 0.0 0.0 0.08517124202662915 3.356502148832676E-5 40-41 0.0 0.0 0.0 0.12157250783071952 3.356502148832676E-5 42-43 0.0 0.0 0.0 0.1595681121555054 6.713004297665352E-5 44-45 0.0 0.0 0.0 0.2069954875185111 6.713004297665352E-5 46-47 0.0 0.0 0.0 0.26367002630155084 6.713004297665352E-5 48-49 0.0 0.0 0.0 0.3268729617640701 6.713004297665352E-5 50-51 0.0 0.0 0.0 0.397426636932533 6.713004297665352E-5 52-53 0.0 0.0 0.0 0.4691383053423431 6.713004297665352E-5 54-55 0.0 0.0 0.0 0.5470595027274936 6.713004297665352E-5 56-57 0.0 0.0 0.0 0.6356208119244438 6.713004297665352E-5 58-59 0.0 0.0 0.0 0.7221850023428384 6.713004297665352E-5 60-61 0.0 0.0 0.0 0.8124413451249491 6.713004297665352E-5 62-63 0.0 0.0 0.0 0.9019256924128283 6.713004297665352E-5 64-65 0.0 0.0 0.0 0.999381732304185 6.713004297665352E-5 66-67 0.0 0.0 0.0 1.104356337008927 6.713004297665352E-5 68-69 0.0 0.0 0.0 1.2285804815372243 6.713004297665352E-5 70-71 0.0 0.0 0.0 1.3913037057126325 6.713004297665352E-5 72-73 0.0 0.0 0.0 1.5711954383793196 8.39125537208169E-5 74-75 0.0 0.0 0.0 1.773676430507651 1.0069506446498027E-4 76-77 0.0 0.0 0.0 1.9998039802745082 1.0069506446498027E-4 78-79 0.0 0.0 0.0 2.2562239619345803 1.0069506446498027E-4 80-81 0.0 0.0 0.0 2.539495960785314 1.0069506446498027E-4 82-83 0.0 0.0 0.0 2.8357744054627743 1.0069506446498027E-4 84-85 0.0 0.0 0.0 3.1341674464939993 1.0069506446498027E-4 86-87 0.0 0.0 0.0 3.438283323688984 1.0069506446498027E-4 88-89 0.0 0.0 0.0 3.7664317562696104 1.0069506446498027E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12590 0.0 44.152287 1 GTATCAA 20630 0.0 37.144257 1 TATCAAC 27170 0.0 28.178852 2 ATCAACG 27745 0.0 27.455025 3 TCAACGC 28155 0.0 27.055216 4 CAACGCA 28580 0.0 26.686043 5 AACGCAG 29035 0.0 26.234781 6 ACGCAGA 32965 0.0 22.992178 7 AGTACTT 20635 0.0 22.957434 12-13 CGCAGAG 33050 0.0 22.875713 8 TAAGGTG 1195 0.0 22.595787 5 GAGTACT 20225 0.0 22.415644 12-13 GTAAGGT 1080 0.0 22.370052 4 GTACATG 15705 0.0 22.027533 1 GCAGAGT 33640 0.0 21.700006 9 AGAGTAC 29710 0.0 21.294222 10-11 GTACTTT 21330 0.0 21.154459 14-15 TACATGG 16050 0.0 21.076319 2 GGTAAGG 1035 0.0 21.054165 3 TACTTTT 22730 0.0 20.789368 14-15 >>END_MODULE