FastQCFastQC Report
Fri 27 May 2016
SRR939366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940371
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184110.6261454762001122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125720.4275650929763625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96350.32767973837315084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39380.13392867770767702No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.10515679824076622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA190900.041.4811481
GGTATCA113400.040.3650741
TATCAAC241700.032.576192
ATCAACG251050.031.5241133
TCAACGC255800.030.9568584
CAACGCA257350.030.4831165
AACGCAG262450.029.8826056
GTACATG147850.026.9156171
ACGCAGA292450.026.4037657
CGCAGAG294050.025.9958848
TACATGG152050.025.7149092
GCAGAGT290650.024.8657449
ACATGGG157150.024.7056523
GAGTACT168450.023.29402712-13
AGTACTT170950.023.10798612-13
AGAGTAC247250.022.18259410-11
GTACTTT181500.021.76263814-15
CATGGGG75800.021.4473154
TACTTTT198250.020.27095214-15
ACTTTTT216000.019.86934516-17
Produced by FastQC (version 0.11.5)