FastQCFastQC Report
Fri 27 May 2016
SRR939366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2940371
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230330.7833365245406108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191720.6520265639948156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148960.5066027382258906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58220.19800222488930821No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44980.15297389343045487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41540.14127468948646277No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG33910.11532558306417795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117250.044.969151
GTATCAA192700.037.656441
TATCAAC257300.028.2152442
ATCAACG263700.027.5091883
TCAACGC267050.027.1336484
CAACGCA272350.026.6187175
AACGCAG279100.026.076886
GTACATG161500.024.2140921
TACATGG164550.023.212942
ACGCAGA320100.022.6923857
CGCAGAG321050.022.59578
AGTACTT195450.022.49555212-13
ACATGGG165500.021.9589313
GAGTACT194600.021.75339312-13
GCAGAGT327550.021.1050789
AGAGTAC286100.021.02841210-11
GTACTTT204900.020.60204714-15
CATGGGG80400.019.8900384
TACTTTT221800.019.73757614-15
CATGGGA81400.019.0627294
Produced by FastQC (version 0.11.5)