FastQCFastQC Report
Fri 27 May 2016
SRR939363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736757
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129680.3470388896040069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95810.2563987971388024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.1715658791834738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99650.039.96011
GTATCAA165750.038.9639971
TATCAAC209400.030.8803672
ATCAACG209850.030.4051783
TCAACGC212950.029.93974
CAACGCA213950.029.7076725
AACGCAG218800.029.1234426
ACGCAGA245000.025.309887
GTACATG147450.025.0720841
CGCAGAG245650.025.0713738
TACATGG144550.024.7473752
GCAGAGT248000.022.8648669
ACATGGG153400.022.4208153
GAGTACT136700.022.2832612-13
GTACTTT147850.020.9838914-15
AGAGTAC210800.020.89682810-11
AGTACTT143200.020.5192612-13
CATGGGA91050.019.6087464
CAGAGTA242300.019.0608210-11
ACTTTTT186200.018.53331416-17
Produced by FastQC (version 0.11.5)