FastQCFastQC Report
Fri 27 May 2016
SRR939363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736757
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183180.49021116438665935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158140.42320118755380665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110440.29555039302796515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39710.10626861741344165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113100.041.152751
GTATCAA180200.034.824361
ATCAACG229900.026.933263
TATCAAC232600.026.8493392
TCAACGC233350.026.5147614
CAACGCA236900.026.1174325
AACGCAG242600.025.5233176
ACGCAGA279300.022.1695567
CGCAGAG279300.022.0677818
GTACATG161250.021.8687111
TACATGG160900.021.1149962
GAGTACT159750.021.01234812-13
ACATGGG163200.020.1751613
AGTACTT167350.020.17133912-13
GCAGAGT296650.020.0744329
AGAGTAC248700.020.04001810-11
GTACTTT169650.019.64652814-15
AATACCG2702.3734174E-819.3010645
CAGAGTA288450.017.8368210-11
TACTTTT192850.017.27075214-15
Produced by FastQC (version 0.11.5)