FastQCFastQC Report
Fri 27 May 2016
SRR939362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707553
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129040.34804627202901756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89460.24129122361838118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.17340278075593255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95400.042.3502271
GTATCAA161100.038.5709151
ATCAACG206800.029.9743523
TATCAAC204100.029.8891892
TCAACGC210750.029.6842164
CAACGCA211750.029.090695
AACGCAG215850.028.3326636
ACGCAGA238550.025.0469957
GTACATG141450.024.7568171
CGCAGAG241850.024.5849178
TACATGG139750.024.2939622
GCAGAGT248050.022.0735939
GAGTACT134150.022.00343912-13
ACATGGG147950.021.8594263
AGAGTAC205150.021.02789310-11
GTACTTT147950.020.5254714-15
AGTACTT144600.020.16393712-13
CAGAGTA239400.018.86022610-11
CATGGGA89800.018.7969024
CATGGGG66200.017.934974
Produced by FastQC (version 0.11.5)