FastQCFastQC Report
Fri 27 May 2016
SRR939362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707553
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178830.4823396995268847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151170.40773523669115447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108580.2928616259834991No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38430.1036532721177553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112950.041.3876881
GTATCAA179550.034.441351
ATCAACG224600.027.2073693
TCAACGC227700.026.8646834
TATCAAC229300.026.7578432
CAACGCA232600.026.2538855
AACGCAG238750.025.6173236
GTACATG156900.022.6255841
TACATGG156050.022.3501382
ACGCAGA275350.022.126137
CGCAGAG276900.021.9166628
GAGTACT162500.021.35147912-13
ACATGGG158550.020.6772253
GCAGAGT290000.020.3217249
GTACTTT170350.020.13106214-15
AGAGTAC248350.019.8898910-11
AGTACTT170450.019.72993512-13
CAGAGTA281850.018.06404910-11
CGTATAG2152.4144874E-517.6592671
TACTTTT193600.017.35845814-15
Produced by FastQC (version 0.11.5)