##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3707553 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.85712625011699 31.0 31.0 31.0 28.0 34.0 2 31.130974796584162 31.0 31.0 34.0 28.0 34.0 3 31.133077800910737 31.0 31.0 34.0 28.0 34.0 4 35.04796371083569 35.0 35.0 37.0 32.0 37.0 5 34.61777835677602 35.0 35.0 37.0 30.0 37.0 6 34.67094442075407 35.0 35.0 37.0 31.0 37.0 7 34.51429851441099 35.0 35.0 37.0 30.0 37.0 8 34.41050336974279 35.0 35.0 37.0 30.0 37.0 9 35.91365760651298 37.0 35.0 39.0 30.0 39.0 10-11 35.70896518539317 37.0 35.0 39.0 30.0 39.0 12-13 35.69471198388803 37.0 35.0 39.0 30.0 39.0 14-15 36.52575256510157 38.0 35.0 40.0 30.0 41.0 16-17 36.443970726783945 38.0 35.0 40.0 30.0 41.0 18-19 36.44426485609242 38.0 35.0 40.0 30.0 41.0 20-21 36.32414344447672 38.0 34.5 40.0 29.5 41.0 22-23 36.159162660655156 38.0 34.0 40.0 29.0 41.0 24-25 36.00073067060673 38.0 34.0 40.0 29.0 41.0 26-27 35.532775526068 38.0 34.0 40.0 27.0 41.0 28-29 35.328980057736196 38.0 34.0 40.0 27.0 41.0 30-31 35.026254513421655 37.5 33.0 40.0 27.0 41.0 32-33 34.793282523540455 37.0 33.0 40.0 25.5 41.0 34-35 35.03512990913414 38.0 33.0 40.0 26.5 41.0 36-37 34.8701235019432 38.0 33.0 40.0 25.5 41.0 38-39 34.69149773448957 38.0 33.0 40.0 25.0 41.0 40-41 34.52945878319204 37.0 33.0 40.0 25.0 41.0 42-43 34.367362111883494 37.0 33.0 40.0 24.5 41.0 44-45 33.97760180366943 37.0 33.0 40.0 24.0 41.0 46-47 33.580093797715094 37.0 32.0 40.0 23.0 41.0 48-49 33.19221330079435 37.0 31.0 40.0 21.0 41.0 50-51 32.953643953302894 37.0 31.0 39.0 20.0 40.5 52-53 32.84306926967733 36.0 31.0 39.0 20.0 40.0 54-55 32.5095042471409 36.0 31.0 39.0 18.5 40.0 56-57 32.003687742292556 35.5 30.0 39.0 16.0 40.0 58-59 31.547336612585177 35.0 29.0 39.0 13.5 40.0 60-61 31.17679652859986 35.0 29.0 38.0 12.0 40.0 62-63 31.024459798686628 35.0 29.0 38.0 10.5 40.0 64-65 30.569060240001964 34.0 28.0 38.0 8.0 40.0 66-67 29.701577293702883 34.0 27.0 37.5 7.0 39.5 68-69 26.264982725803243 31.0 18.5 36.0 2.0 39.0 70-71 24.954589590492706 29.5 13.0 35.0 2.0 38.0 72-73 25.965224367662444 31.0 19.5 35.0 2.0 38.0 74-75 26.715042509169795 31.0 22.5 35.0 2.0 37.5 76-77 26.381530216830345 30.5 23.0 34.0 2.0 36.5 78-79 27.4980626035555 32.0 26.0 35.0 2.0 37.0 80-81 27.50463472808076 32.0 26.0 35.0 2.0 36.5 82-83 27.17493074272977 32.0 25.5 35.0 2.0 36.0 84-85 26.803276446756122 32.0 25.0 35.0 2.0 36.0 86-87 26.34976384693624 32.0 24.5 34.0 2.0 35.0 88-89 25.865920460206503 31.5 23.5 34.0 2.0 35.0 90-91 25.262284315288277 31.0 20.5 34.0 2.0 35.0 92-93 24.686346223506447 31.0 19.0 34.0 2.0 35.0 94-95 23.836361341294378 31.0 12.0 34.0 2.0 35.0 96-97 23.157684596821678 30.0 4.5 34.0 2.0 35.0 98-99 22.291895085518668 30.0 2.0 34.0 2.0 35.0 100-101 20.873933427249725 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2852940231715735 1101 2 0.26649512045593937 1101 3 0.2484472474449788 1101 4 0.3091773315146682 1101 5 0.49127859250142336 1101 6 0.3700604350467387 1101 7 0.5198301140327004 1101 8 0.6045620305581707 1101 9 0.6919332704710328 1101 10-11 0.7037702671369672 1101 12-13 0.6958692072867336 1101 14-15 0.6982605011592184 1101 16-17 0.6530855147122878 1101 18-19 0.4865631097765615 1101 20-21 0.7119386830365784 1101 22-23 0.7148675449688042 1101 24-25 0.6449786620394704 1101 26-27 0.7741961965732571 1101 28-29 0.7830934864055408 1101 30-31 0.5215150588123265 1101 32-33 0.8290571889316709 1101 34-35 0.7176276631243113 1101 36-37 0.9120972413685706 1101 38-39 0.8887398079490723 1101 40-41 0.7886878131868755 1101 42-43 0.4553362804073018 1101 44-45 0.7058387583256405 1101 46-47 0.7022789327722947 1101 48-49 0.7765462572532797 1101 50-51 0.5345740621815622 1101 52-53 0.42499159649043605 1101 54-55 0.43064623631326526 1101 56-57 0.6127105281751746 1101 58-59 0.6018340094432624 1101 60-61 0.45188304235387733 1101 62-63 0.13678091266996262 1101 64-65 0.0791730736637426 1101 66-67 -0.009890987357355385 1101 68-69 -6.990411916149487 1101 70-71 -7.750991872802132 1101 72-73 -4.956212149642191 1101 74-75 -2.8946521382628916 1101 76-77 -0.11072878616472082 1101 78-79 0.06318705379107925 1101 80-81 3.1425881927305E-4 1101 82-83 -0.17434705131293882 1101 84-85 -0.3265795487358538 1101 86-87 -0.5191410706186765 1101 88-89 -0.4911421189201839 1101 90-91 -0.4907150691557298 1101 92-93 -0.41036396111872975 1101 94-95 -0.5311640479216173 1101 96-97 -0.7133054838283321 1101 98-99 -0.6821864680971359 1101 100-101 -0.6641266048529033 1102 1 0.3940418955375051 1102 2 0.3235642595732422 1102 3 0.40752415392497454 1102 4 0.3695260237688771 1102 5 0.4669600347376601 1102 6 0.3924173741304742 1102 7 0.4386150252114689 1102 8 0.5862649768818571 1102 9 0.5726305351127579 1102 10-11 0.5717493743697233 1102 12-13 0.6203789524501104 1102 14-15 0.704746788407796 1102 16-17 0.6007774812455082 1102 18-19 0.5718833336875733 1102 20-21 0.7727200941074699 1102 22-23 0.7107884808880627 1102 24-25 0.5866014774687187 1102 26-27 0.6340150779771321 1102 28-29 0.5252598973565554 1102 30-31 0.6129200164993875 1102 32-33 0.6958801817636413 1102 34-35 0.534985860808483 1102 36-37 0.6854490741305383 1102 38-39 0.794121523481401 1102 40-41 0.7283942596184474 1102 42-43 0.517375992247743 1102 44-45 0.5220869902916903 1102 46-47 0.6349183377539589 1102 48-49 0.745443843137771 1102 50-51 0.5978304211104302 1102 52-53 0.4379683663223872 1102 54-55 0.1898034534877624 1102 56-57 0.5824121200050563 1102 58-59 0.501485796655885 1102 60-61 0.722010160997943 1102 62-63 0.5038745045957427 1102 64-65 0.4495246427189521 1102 66-67 0.33833888102899934 1102 68-69 -6.763651973206041 1102 70-71 -7.536480809104962 1102 72-73 -1.292660918611098 1102 74-75 0.45506947130726516 1102 76-77 0.8807184245369797 1102 78-79 0.673465923687754 1102 80-81 0.38218142253322185 1102 82-83 0.30212581086432166 1102 84-85 0.13902290133966488 1102 86-87 0.12707713473696458 1102 88-89 -0.01999493458916035 1102 90-91 0.0027911519264272044 1102 92-93 0.0015061393295745518 1102 94-95 0.13553038786578853 1102 96-97 -0.14297039644550225 1102 98-99 0.06525617568297903 1102 100-101 -0.13954493966459225 1103 1 0.41704431684500776 1103 2 0.2593996106628289 1103 3 0.35934013455451463 1103 4 0.3666204547616161 1103 5 0.40037407832119243 1103 6 0.5185674951958532 1103 7 0.441520594218737 1103 8 0.47972744661551303 1103 9 0.5268678232483452 1103 10-11 0.5850665656530154 1103 12-13 0.5144467490602835 1103 14-15 0.6043835922818843 1103 16-17 0.6760801446837661 1103 18-19 0.6819317598376884 1103 20-21 0.6738096824851922 1103 22-23 0.5984398126071895 1103 24-25 0.634059104587358 1103 26-27 0.7917632619965076 1103 28-29 0.7560105026834307 1103 30-31 0.7531137210998651 1103 32-33 0.8151295764367603 1103 34-35 0.7529035363532799 1103 36-37 0.8354490741305369 1103 38-39 1.0091336300189298 1103 40-41 0.9415903855264318 1103 42-43 0.7404994789305519 1103 44-45 0.9464211307275292 1103 46-47 0.9442403716522634 1103 48-49 1.0513760465276007 1103 50-51 0.7589684356382804 1103 52-53 0.6558860418671841 1103 54-55 0.48931919198656004 1103 56-57 0.841249892402157 1103 58-59 0.9973695738955932 1103 60-61 0.9359326791577516 1103 62-63 0.5213079186393266 1103 64-65 0.49782972746471543 1103 66-67 0.3562565565737934 1103 68-69 -6.422852941729044 1103 70-71 -7.561420276417312 1103 72-73 -0.8799490542043173 1103 74-75 0.5285561541159822 1103 76-77 0.8552946957234191 1103 78-79 0.5950155604916283 1103 80-81 0.48084970340489264 1103 82-83 0.5268231474260645 1103 84-85 0.14628682385782454 1103 86-87 0.1594015899427781 1103 88-89 0.2927169298176189 1103 90-91 0.0748250502315102 1103 92-93 0.2500533548259405 1103 94-95 0.5653124701902428 1103 96-97 0.5381434050072826 1103 98-99 0.6176774831890341 1103 100-101 0.7087601450811718 1104 1 0.21722221358329818 1104 2 0.30520825392624573 1104 3 0.21918653249529285 1104 4 0.2390210465072542 1104 5 0.5045464912701192 1104 6 0.2671291079708453 1104 7 0.32772695210360325 1104 8 0.47158546496709164 1104 9 0.5615258616511909 1104 10-11 0.4416505519155649 1104 12-13 0.32590097912063243 1104 14-15 0.5974256265066131 1104 16-17 0.5152406842494628 1104 18-19 0.5482880009705369 1104 20-21 0.5738953084054259 1104 22-23 0.4639594377883185 1104 24-25 0.4175455425840511 1104 26-27 0.6197344347976497 1104 28-29 0.5449503034064449 1104 30-31 0.6892497246717966 1104 32-33 0.6529393947978974 1104 34-35 0.3240166500008499 1104 36-37 0.6718455693549572 1104 38-39 0.6191651524815995 1104 40-41 0.5594030250547135 1104 42-43 0.32628406066920235 1104 44-45 0.40980278917744783 1104 46-47 0.46927001139388125 1104 48-49 0.5602309557664569 1104 50-51 0.3545343652653088 1104 52-53 0.4548068288562348 1104 54-55 0.3762659896704008 1104 56-57 0.4644114415260212 1104 58-59 0.5939007040761375 1104 60-61 0.605692623480504 1104 62-63 0.3696943159505892 1104 64-65 0.4362905126584806 1104 66-67 0.20294961242241172 1104 68-69 -6.2707384652945315 1104 70-71 -4.149512444647474 1104 72-73 0.33082628879481746 1104 74-75 1.0563293206196356 1104 76-77 1.1476705497967998 1104 78-79 0.5934082529692581 1104 80-81 0.3681142321242561 1104 82-83 0.21094143360199524 1104 84-85 0.302008547194589 1104 86-87 0.23580584920570757 1104 88-89 0.2995242196202632 1104 90-91 0.27854240425651255 1104 92-93 0.35086933662125475 1104 94-95 0.4866824033099313 1104 96-97 0.20456796035286828 1104 98-99 0.43686864757331634 1104 100-101 0.5170068130146426 1105 1 0.1461028997581586 1105 2 0.19035242554128828 1105 3 0.15655346129216952 1105 4 0.16716170522085605 1105 5 0.3185766986561305 1105 6 0.19599552855608948 1105 7 0.18065407277266132 1105 8 0.28368803455641967 1105 9 0.35083558543371396 1105 10-11 0.345119764989974 1105 12-13 0.3303313362953304 1105 14-15 0.33945740310748107 1105 16-17 0.39466346282968345 1105 18-19 0.4259471960346488 1105 20-21 0.40423643431708456 1105 22-23 0.3634066461907466 1105 24-25 0.35129971081101274 1105 26-27 0.5665897638869737 1105 28-29 0.4911217757936157 1105 30-31 0.4662259546330958 1105 32-33 0.4504482662114384 1105 34-35 0.5565297119313399 1105 36-37 0.6450966972281336 1105 38-39 0.5297867755490557 1105 40-41 0.629111828706705 1105 42-43 0.502030775125597 1105 44-45 0.5117865977332414 1105 46-47 0.5648797761899758 1105 48-49 0.6580126565813771 1105 50-51 0.621268793482642 1105 52-53 0.38266752537364823 1105 54-55 0.4466194196531035 1105 56-57 0.6486943281680801 1105 58-59 0.705269487399665 1105 60-61 0.6010616564518436 1105 62-63 0.4128833885089449 1105 64-65 0.3863217186357666 1105 66-67 0.37877211282363277 1105 68-69 -3.18214112939339 1105 70-71 -3.605047493417956 1105 72-73 0.6243604103019749 1105 74-75 0.964556107830532 1105 76-77 0.9049174693210418 1105 78-79 0.4979554096589247 1105 80-81 0.2998195050428194 1105 82-83 0.3515742908212758 1105 84-85 0.2966874822683252 1105 86-87 0.2874838391841301 1105 88-89 0.27593339239438563 1105 90-91 0.2559162875283256 1105 92-93 0.17723303782847566 1105 94-95 0.29484142897015886 1105 96-97 0.35318241496154457 1105 98-99 0.37626574809222646 1105 100-101 0.5152726522771118 1106 1 0.09848489158850171 1106 2 0.17659177872103626 1106 3 0.14464211967276341 1106 4 0.146991938188485 1106 5 0.2294209207415605 1106 6 0.1671959616322809 1106 7 0.30914614977756827 1106 8 0.3402993588586014 1106 9 0.3342750777333734 1106 10-11 0.3928594303163777 1106 12-13 0.15570718560344687 1106 14-15 0.21521474073664848 1106 16-17 0.3473821946847977 1106 18-19 0.19575031808326315 1106 20-21 0.29134528691255923 1106 22-23 0.3217831634490409 1106 24-25 0.2704723548314121 1106 26-27 0.4755248708535902 1106 28-29 0.3755608649947959 1106 30-31 0.4458083620768889 1106 32-33 0.5229904205617757 1106 34-35 0.4243601253473912 1106 36-37 0.4956776974653394 1106 38-39 0.38697291536455936 1106 40-41 0.4309849996655757 1106 42-43 0.375743806848142 1106 44-45 0.44421157703260405 1106 46-47 0.4896643658953863 1106 48-49 0.5726500789630933 1106 50-51 0.3541265579305275 1106 52-53 0.12207134709643697 1106 54-55 0.2132034293041798 1106 56-57 0.3908507158236567 1106 58-59 0.6186672000704867 1106 60-61 0.7889871836449842 1106 62-63 0.39274592756365934 1106 64-65 0.5800754385652702 1106 66-67 0.5998256405173699 1106 68-69 -2.9838516057335482 1106 70-71 -3.02133834715724 1106 72-73 0.9081546874678281 1106 74-75 1.3824970459003474 1106 76-77 1.3752070744737175 1106 78-79 0.9368488825196302 1106 80-81 0.7089209447010134 1106 82-83 0.5053434070751663 1106 84-85 0.5459295261667663 1106 86-87 0.6763516272503765 1106 88-89 0.6164284904550037 1106 90-91 0.6048032555147387 1106 92-93 0.40464897287869306 1106 94-95 0.622562089003889 1106 96-97 0.7343300668765558 1106 98-99 0.7444638979138851 1106 100-101 0.8092634290347718 1107 1 0.042243310235395626 1107 2 0.09029487187045859 1107 3 0.02282728123862654 1107 4 -0.020460310217742972 1107 5 0.20401086144296698 1107 6 0.12673524230563515 1107 7 0.16205776192983024 1107 8 0.10781491886691441 1107 9 0.2617637656203726 1107 10-11 0.13763851059100318 1107 12-13 0.16916292750826756 1107 14-15 0.041119502524445295 1107 16-17 -0.04258614030297281 1107 18-19 0.17304549415062098 1107 20-21 0.24341226334141908 1107 22-23 0.3008485466485027 1107 24-25 0.2896895753415478 1107 26-27 0.4576533442794499 1107 28-29 0.3964586063180846 1107 30-31 0.339938774765983 1107 32-33 0.42251862323995937 1107 34-35 0.19454886576890118 1107 36-37 0.5464687393956069 1107 38-39 0.5691279170271812 1107 40-41 0.49010339021218385 1107 42-43 0.21224647640548255 1107 44-45 0.49045999616154745 1107 46-47 0.559943683051209 1107 48-49 0.5265338404582778 1107 50-51 0.4616679768288918 1107 52-53 0.3226224220887488 1107 54-55 0.29755011160122535 1107 56-57 0.6392297760793006 1107 58-59 0.8151115566333225 1107 60-61 0.6598708164905034 1107 62-63 0.42233381570415673 1107 64-65 0.19407715860210573 1107 66-67 0.3656563811556488 1107 68-69 -2.476824417054228 1107 70-71 -2.6874508274951836 1107 72-73 1.1367994722283399 1107 74-75 1.514925297612784 1107 76-77 1.0188809957738982 1107 78-79 0.8540254245578289 1107 80-81 0.6145717968745643 1107 82-83 0.6365895556449601 1107 84-85 0.5045971516163128 1107 86-87 0.4714764496662447 1107 88-89 0.5193341306835997 1107 90-91 0.5984687792770096 1107 92-93 0.5609809774462171 1107 94-95 0.79520991100188 1107 96-97 0.910802222820827 1107 98-99 0.9671021556228112 1107 100-101 1.255820945107537 1108 1 0.013177858213339277 1108 2 0.03364611991906585 1108 3 0.0513922613662956 1108 4 0.006772939120821775 1108 5 -0.08387154546318953 1108 6 -0.14224182607470937 1108 7 0.18078394270686715 1108 8 0.24726207925156274 1108 9 0.28931038207879567 1108 10-11 0.3674201803628421 1108 12-13 0.2777680623676986 1108 14-15 0.2902974144974664 1108 16-17 0.3469972797853984 1108 18-19 0.21847304572602155 1108 20-21 0.2560271177312501 1108 22-23 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30-31 0.03378416362978953 2312 32-33 0.6595404698178982 2312 34-35 0.594021029205102 2312 36-37 0.5784866419365358 2312 38-39 0.4956167747155149 2312 40-41 0.33444823225203635 2312 42-43 0.02846481419091873 2312 44-45 0.6442492272795093 2312 46-47 0.8953168993596492 2312 48-49 1.066086897363796 2312 50-51 1.0866597940105578 2312 52-53 0.7516503960913283 2312 54-55 0.8987612745612807 2312 56-57 0.8023567020948938 2312 58-59 1.0576895368564685 2312 60-61 1.045455393775061 2312 62-63 0.9952824604991655 2312 64-65 1.150034869145614 2312 66-67 1.494021758568401 2312 68-69 4.28644164129928 2312 70-71 5.0120300398542525 2312 72-73 2.9283002841593273 2312 74-75 2.300906167567689 2312 76-77 2.3868771953636667 2312 78-79 1.7121662540366849 2312 80-81 1.5150531745713138 2312 82-83 1.4868456086507358 2312 84-85 1.606066215840606 2312 86-87 1.5854173503396964 2312 88-89 1.5865784147610889 2312 90-91 1.7170096858779118 2312 92-93 2.1022358163008725 2312 94-95 2.313962528689533 2312 96-97 2.3823813813698145 2312 98-99 2.3173520613372034 2312 100-101 2.2184206615016393 2313 1 -0.27551052097869544 2313 2 -0.2590545403063338 2313 3 -0.10942718677313934 2313 4 0.05537544696348107 2313 5 5.304182760852427E-4 2313 6 0.13133017763333754 2313 7 -2.75396365596443 2313 8 -2.993422675204396 2313 9 -0.9478764077544994 2313 10-11 -0.03002820035391096 2313 12-13 -0.0718039366247254 2313 14-15 -0.42635648726134434 2313 16-17 -0.19196576433272128 2313 18-19 0.030655876407536198 2313 20-21 -2.594143607180598 2313 22-23 -0.6400354352014475 2313 24-25 -0.08460393980126213 2313 26-27 -1.5374055330917713 2313 28-29 -1.683420441007776 2313 30-31 -0.921381852411983 2313 32-33 -0.9504293804014878 2313 34-35 -1.2591714615528815 2313 36-37 -2.0253155580291633 2313 38-39 -1.7832580770217277 2313 40-41 -0.9589186247707246 2313 42-43 -5.487382129700791E-4 2313 44-45 -0.027828426170934506 2313 46-47 -0.2902660520939051 2313 48-49 -2.161539618177521 2313 50-51 -0.44774033667808055 2313 52-53 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2314 10-11 -0.23516191247885843 2314 12-13 -2.271517872651259 2314 14-15 -0.6462779937163106 2314 16-17 -1.7502040713846938 2314 18-19 -0.3911545753116812 2314 20-21 -0.7518873646136797 2314 22-23 -0.43514084306799106 2314 24-25 -0.29218830183294386 2314 26-27 -1.8116764077878997 2314 28-29 -0.779417852977538 2314 30-31 -0.19765696428894586 2314 32-33 0.1722045123712661 2314 34-35 0.010004607517835495 2314 36-37 -0.4614896850994086 2314 38-39 -0.4383274159524859 2314 40-41 -0.44555875470806683 2314 42-43 -0.7182162784099191 2314 44-45 -3.751221988147499 2314 46-47 -4.151580612658243 2314 48-49 -3.8514341364873843 2314 50-51 -4.146905191407434 2314 52-53 -1.2126761254119671 2314 54-55 -1.447533761636322 2314 56-57 -1.2393396089847606 2314 58-59 -1.9562553977089365 2314 60-61 -1.2733013002881073 2314 62-63 -1.2728671159860525 2314 64-65 -1.3403832457899831 2314 66-67 -0.9015899764435886 2314 68-69 -1.3565114712728672 2314 70-71 1.6355266880592865 2314 72-73 1.221938684346135 2314 74-75 0.6393688458091589 2314 76-77 0.7833601720721788 2314 78-79 -1.5614837545981963 2314 80-81 -0.9489187794829874 2314 82-83 0.017348442757963767 2314 84-85 0.05339564442199318 2314 86-87 -0.23162635272078447 2314 88-89 -0.2149792496523979 2314 90-91 0.22047402826044404 2314 92-93 -0.36163843787609906 2314 94-95 0.1612350704025154 2314 96-97 0.4138699302144815 2314 98-99 0.13721451734185663 2314 100-101 0.1624309293128121 2315 1 -0.29999509745646336 2315 2 -0.21795280658528426 2315 3 -0.31336022028404287 2315 4 -0.10242183136020344 2315 5 -0.21014308812817717 2315 6 -0.19690387972793388 2315 7 -0.8274871472792285 2315 8 -2.5095598993863035 2315 9 -0.6229076366966027 2315 10-11 -1.170794001420063 2315 12-13 -0.5747707117347574 2315 14-15 -0.297121884712503 2315 16-17 -0.26038839184995766 2315 18-19 -0.04355150671467101 2315 20-21 -0.4358192732324184 2315 22-23 -0.15913405637476075 2315 24-25 -4.958145829403755 2315 26-27 -0.9775602364665019 2315 28-29 -0.741831572668417 2315 30-31 -0.9716198315997886 2315 32-33 -0.06982878694402217 2315 34-35 -2.004430112179847 2315 36-37 -3.367726643386767 2315 38-39 -1.0181125266926827 2315 40-41 -0.008721232451414096 2315 42-43 -1.4215632808505916 2315 44-45 -4.8094618027955605 2315 46-47 -3.171729910419714 2315 48-49 -2.414712913042454 2315 50-51 -0.9538190448806247 2315 52-53 0.0022036963986593605 2315 54-55 -0.9341162310577289 2315 56-57 -1.0125610004569765 2315 58-59 -0.4709826953041336 2315 60-61 -1.2895617308216671 2315 62-63 -0.24176515199567206 2315 64-65 0.24631867875679347 2315 66-67 0.38461928467576456 2315 68-69 3.3291566161475323 2315 70-71 3.8522485695852993 2315 72-73 -2.2104624637268557 2315 74-75 -1.6421411697104134 2315 76-77 -0.16563281946746145 2315 78-79 -0.1819909329425009 2315 80-81 -0.28309637514352914 2315 82-83 -0.8873803442872052 2315 84-85 -0.4616523903395411 2315 86-87 -0.015497363052656254 2315 88-89 -0.02864374630185651 2315 90-91 0.10327159282407905 2315 92-93 -0.7628640262139932 2315 94-95 -0.030722539273707383 2315 96-97 -0.04086755216534499 2315 98-99 -2.3429989546712378 2315 100-101 -3.6657857204686053 2316 1 -0.5027540673517628 2316 2 -0.3910367041550913 2316 3 -0.9336248883400273 2316 4 -0.3254407731529483 2316 5 -0.29365775827650964 2316 6 -0.6057174998896144 2316 7 -0.6340949213279785 2316 8 -1.231258337192891 2316 9 -5.266305767718496 2316 10-11 -1.583093240642384 2316 12-13 -0.7984023627274155 2316 14-15 -0.5355973489115016 2316 16-17 -0.43467969037964593 2316 18-19 -0.5621108647637527 2316 20-21 -3.692516762507978 2316 22-23 -1.0996656940693867 2316 24-25 -0.5006896547922111 2316 26-27 -0.6777486407676179 2316 28-29 -0.04305573565952869 2316 30-31 -0.09110870356390421 2316 32-33 -1.4171768987628113 2316 34-35 -0.594129762618266 2316 36-37 -0.36658026923963405 2316 38-39 -5.41209116800567 2316 40-41 -5.189812894026705 2316 42-43 -3.823924681403156 2316 44-45 -2.2979271539798773 2316 46-47 -2.5921517602970106 2316 48-49 -3.0291114513747317 2316 50-51 -1.5224450325850611 2316 52-53 -1.1442446128447799 2316 54-55 -0.32391529126805807 2316 56-57 -3.78543303618126 2316 58-59 -0.9950188901324069 2316 60-61 -0.5713312433604081 2316 62-63 -0.3814298369764302 2316 64-65 -0.09600372973088511 2316 66-67 -0.12631602271803288 2316 68-69 2.6125760612196807 2316 70-71 3.691264138513283 2316 72-73 2.289102560400238 2316 74-75 1.0387460229818046 2316 76-77 -1.4438440619780195 2316 78-79 -0.1446099759118873 2316 80-81 -0.06496155447634067 2316 82-83 0.17754474500955197 2316 84-85 0.3312351613065836 2316 86-87 0.17127618539263878 2316 88-89 -0.03394622075894205 2316 90-91 -0.1977164835828873 2316 92-93 -0.2204947956307528 2316 94-95 0.01148093086197477 2316 96-97 0.19438546383786104 2316 98-99 0.23758015142195887 2316 100-101 0.4359441165049489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12.0 3 60.0 4 131.0 5 278.0 6 705.0 7 1745.0 8 3955.0 9 6391.0 10 10183.0 11 14627.0 12 19410.0 13 24456.0 14 30034.0 15 37684.0 16 44607.0 17 49558.0 18 46783.0 19 42035.0 20 42148.0 21 42772.0 22 45307.0 23 50423.0 24 59937.0 25 74138.0 26 92773.0 27 114568.0 28 140253.0 29 166825.0 30 194979.0 31 225341.0 32 258237.0 33 298790.0 34 342386.0 35 381511.0 36 388271.0 37 315952.0 38 132221.0 39 8066.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.67009409557612 20.83410781860076 15.977984025415168 22.51781406040796 2 15.800744288683655 21.587285315607808 39.14872150411598 23.46324889159256 3 19.04133198832649 25.27017914843805 30.755676270518993 24.932812592716463 4 13.143609341642486 18.059801500355814 37.254534597056086 31.54205456094561 5 13.346403948911856 38.050703523321175 34.289597478444676 14.313295049322289 6 31.12378973409146 37.43142714345554 17.176881894877834 14.26790122757517 7 27.503989828331516 33.60334970262057 20.75091037134196 18.141750097705952 8 25.95752508460432 35.631156183067375 19.52042222997217 18.890896502356135 9 25.742207865942845 17.10605350752909 20.64625913641693 36.50547949011113 10-11 24.629829893446363 27.37411006361496 27.222524491205768 20.77353555173291 12-13 25.53935169638843 24.67774567214548 28.025479339068117 21.757423292397977 14-15 22.41777797916847 26.047975578501507 26.677420390214245 24.85682605211578 16-17 21.58016466510678 29.290996014861665 27.209580515303333 21.919258804728223 18-19 21.28829585259696 27.5025765000004 30.14486120075554 21.0642664466471 20-21 22.278386849763173 26.6060660494941 30.18852326588453 20.927023834858193 22-23 21.82405116910779 26.640938981394584 30.032505297636618 21.50250455186101 24-25 21.58579097366415 26.787901984615708 30.247654836870296 21.378652204849843 26-27 21.68237251077633 26.94998234626619 30.079076319963878 21.288568822993604 28-29 21.386237476603235 27.11556717101678 30.171674496943435 21.326520855436552 30-31 21.67038341284689 26.828366703132854 30.112643302412597 21.388606581607664 32-33 21.55261445162773 26.895960568864368 29.990071549040643 21.56135343046726 34-35 21.881902083121766 26.968659375652827 29.879683841183695 21.269754700041712 36-37 21.80226896238116 26.830707590648423 29.935437247874507 21.43158619909591 38-39 21.632225612114304 27.040076155805686 29.9592830479493 21.368415184130715 40-41 21.839779487970592 26.87416802205768 29.874451044707186 21.411601445264537 42-43 21.622092923597453 26.981672237119202 29.69282226169056 21.703412577592786 44-45 21.82657470038164 27.086719938151667 29.440557628384152 21.646147733082543 46-47 21.927515319790633 27.34532858914591 28.98423542492469 21.742920666138765 48-49 21.968364035599738 27.421556209811452 28.900123555844072 21.709956198744738 50-51 21.895594329290635 27.57633115172029 28.735116547261647 21.79295797172743 52-53 22.060837838942852 27.63070630514562 28.499191803692565 21.809264052218968 54-55 21.905440638915767 27.520496370802565 28.62231628554112 21.951746704740543 56-57 22.013759678968704 27.716657073300826 28.45409395706983 21.81548929066064 58-59 22.10961795918918 27.52270696265921 28.510867036039105 21.856808042112505 60-61 22.08994946279009 27.55143178420804 28.73426604646445 21.624352706537415 62-63 21.94283467087906 27.51745156979502 28.722921762509763 21.81679199681616 64-65 21.86092649137382 27.470180615646882 28.629899765868604 22.03899312711069 66-67 21.719555901694125 27.610025736177036 28.64979214126776 22.020626220861082 68-69 21.876261609524413 27.132218579911942 29.155819881145856 21.83569992941779 70-71 21.46835456127344 27.109683442887018 29.523343160079722 21.898618835759816 72-73 21.869397614755933 27.31696991335564 28.72787434253391 22.085758129354517 74-75 21.800175815813034 27.591640450590425 28.292407469001162 22.315776264595378 76-77 21.201812067332845 27.776731317210697 28.71861338960258 22.302843225853874 78-79 21.72039231258113 27.679358540119836 28.24849125071081 22.351757896588225 80-81 21.64130009961054 27.820956730508577 28.18076035775639 22.35698281212449 82-83 21.800239298976994 27.883806213142236 27.975242589607646 22.34071189827312 84-85 21.73883081059343 27.741512370861244 27.929467035176664 22.59018978336866 86-87 21.720721729150625 27.73554040002859 28.041084454825842 22.50265341599494 88-89 21.68510543034908 27.719911504723143 27.907663904055045 22.687319160872736 90-91 21.64969114448763 27.790724867069805 28.022841902283464 22.536742086159105 92-93 21.68134049991463 27.75666081259796 27.913280302358018 22.64871838512939 94-95 21.483280380820812 27.571220015901275 28.057570657222534 22.88792894605538 96-97 21.42251687685813 27.50258385679396 28.10353882813983 22.971360438208084 98-99 21.409387898568376 27.51762925173096 27.8448437018232 23.228139147877464 100-101 21.291186836667244 27.45265788987063 28.012437263721058 23.243718009741077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2804.0 1 1948.0 2 1152.5 3 1227.0 4 1150.5 5 1159.5 6 1786.5 7 3433.5 8 5391.0 9 6486.5 10 6042.5 11 5097.0 12 4769.0 13 4779.0 14 5046.5 15 5427.0 16 5841.5 17 6298.5 18 6951.5 19 7725.5 20 8642.5 21 9916.0 22 11596.0 23 13782.5 24 16683.5 25 20472.5 26 25723.0 27 31417.0 28 36487.0 29 42342.0 30 48752.0 31 55152.5 32 61974.0 33 69614.0 34 77859.0 35 85964.5 36 95012.0 37 103247.5 38 108803.0 39 112069.0 40 115038.0 41 119867.0 42 124252.0 43 127519.0 44 130712.5 45 133457.5 46 135753.0 47 137673.5 48 141132.0 49 140685.5 50 133840.0 51 127565.0 52 122362.0 53 117717.0 54 112588.0 55 104418.0 56 94715.5 57 85166.5 58 76078.5 59 66259.0 60 55181.5 61 44427.0 62 35054.5 63 27061.0 64 20623.5 65 15777.5 66 11771.0 67 8913.5 68 6428.5 69 4322.5 70 3155.5 71 2418.5 72 1911.0 73 1534.5 74 1187.0 75 853.5 76 569.0 77 352.0 78 204.0 79 135.0 80 102.5 81 67.0 82 37.0 83 18.0 84 10.0 85 7.0 86 3.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021307854533704578 2 0.017747554788832418 3 3.5063558093437907E-4 4 0.09076067152647582 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0109506189122583 12-13 0.0 14-15 0.0 16-17 7.552150973971242E-4 18-19 1.0788787105673203E-4 20-21 0.0 22-23 9.440188717464052E-5 24-25 0.003641215648164706 26-27 0.003290580067230327 28-29 0.0011463086299777778 30-31 2.4274770987764706E-4 32-33 7.012711618687581E-4 34-35 0.003034346373470588 36-37 0.08556856773187059 38-39 0.09622249499872287 40-41 0.07828613643554118 42-43 0.0819003801159417 44-45 0.0811047070668983 46-47 0.07955381892045778 48-49 0.07418639733538536 50-51 0.07351209814128078 52-53 0.05608820696561856 54-55 0.04787524278142484 56-57 0.02693150981253673 58-59 0.02199563971169124 60-61 0.013013974446218301 62-63 0.002117299469488366 64-65 0.00628446848905464 66-67 0.1553585343216941 68-69 0.7212034460464894 70-71 0.8824823272924217 72-73 0.3575404046820099 74-75 0.05314826247932262 76-77 0.1955197943225626 78-79 0.013728731591969152 80-81 0.0030748043251168627 82-83 0.0015374021625584313 84-85 2.697196776418301E-4 86-87 0.0018340938079644444 88-89 2.832056615239216E-4 90-91 0.0042076269712125495 92-93 0.0043155148422692816 94-95 0.0760204911433498 96-97 0.03691113788528444 98-99 0.013593871753148237 100-101 0.012609394929755555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3707553.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.84225083828218 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55800658263676 33.97369605320028 2 15.04845885105671 14.700012038657587 3 6.1542822710098655 9.017669952307703 4 3.138386014835666 6.1314334785584155 5 1.7485373632242014 4.270125024735248 6 1.1295674166457779 3.3102369061538086 7 0.6971013432457942 2.383359906656017 8 0.4740027018675336 1.8521087090110049 9 0.3389893916936077 1.4901304410554288 >10 1.5707221443157848 13.070407485707749 >50 0.08878046929921597 2.9661111489152163 >100 0.04993353303850494 4.303545495162344 >500 0.0023403538014854074 0.7084927814454985 >1k 7.243952050162246E-4 0.6290213888123368 >5k 0.0 0.0 >10k+ 1.6716812423451326E-4 1.1936491896214718 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17883 0.4823396995268847 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15117 0.40773523669115447 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10858 0.2928616259834991 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3843 0.1036532721177553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.6971967764183006E-5 0.0 0.0 0.0 0.0 3 2.6971967764183006E-5 0.0 0.0 5.394393552836601E-5 0.0 4 2.6971967764183006E-5 0.0 0.0 5.394393552836601E-5 0.0 5 2.6971967764183006E-5 0.0 0.0 5.394393552836601E-5 0.0 6 5.394393552836601E-5 0.0 0.0 5.394393552836601E-5 0.0 7 8.091590329254902E-5 0.0 0.0 5.394393552836601E-5 0.0 8 8.091590329254902E-5 0.0 0.0 1.6183180658509803E-4 0.0 9 8.091590329254902E-5 0.0 0.0 3.5063558093437907E-4 0.0 10-11 8.091590329254902E-5 0.0 0.0 4.5852345199111117E-4 0.0 12-13 1.0788787105673203E-4 0.0 0.0 6.203552585762092E-4 0.0 14-15 1.3485983882091502E-4 0.0 0.0 8.361310006896732E-4 0.0 16-17 1.4834582270300653E-4 0.0 0.0 0.0010249347750389542 0.0 18-19 1.8880377434928104E-4 0.0 0.0 0.0010788787105673202 0.0 20-21 1.8880377434928104E-4 0.0 0.0 0.0011732805977419606 0.0 22-23 2.0228975823137255E-4 0.0 0.0 0.0015104301947942483 0.0 24-25 2.6971967764183004E-4 0.0 0.0 0.0020228975823137255 0.0 26-27 3.2366361317019606E-4 0.0 0.0 0.0025623369375973853 0.0 28-29 4.585234519911111E-4 0.0 0.0 0.003641215648164706 0.0 30-31 5.664113230478431E-4 0.0 0.0 0.007053169570333856 0.0 32-33 5.798973069299346E-4 0.0 0.0 0.013243236172213856 0.0 34-35 6.068692746941176E-4 0.0 0.0 0.02377578958412732 0.0 36-37 6.742991941045751E-4 0.0 0.0 0.040808587227208884 0.0 38-39 7.552150973971242E-4 0.0 0.0 0.06556885363472889 0.0 40-41 7.552150973971242E-4 0.0 0.0 0.09382198986771059 0.0 42-43 8.226450168075817E-4 0.0 0.0 0.12355858432772235 0.0 44-45 9.170469039822222E-4 0.0 0.0 0.16094173164887998 0.0 46-47 0.0010249347750389544 0.0 0.0 0.20711774046116133 0.0 48-49 0.0011058506783315032 0.0 0.0 0.25848585306804783 0.0 50-51 0.0011058506783315032 0.0 0.0 0.3120252090799511 0.0 52-53 0.0011058506783315032 0.0 0.0 0.36882817319132055 0.0 54-55 0.0011058506783315032 0.0 0.0 0.43418125108393596 0.0 56-57 0.0011867665816240522 0.0 0.0 0.5104175179693993 0.0 58-59 0.0012676824849166012 0.0 0.0 0.5880023832430716 0.0 60-61 0.0012676824849166012 0.0 0.0 0.665438902694041 0.0 62-63 0.0012811684687986926 2.6971967764183006E-5 0.0 0.7427135903384253 0.0 64-65 0.0013216264204449673 2.6971967764183006E-5 0.0 0.8304399154914306 0.0 66-67 0.0014564862592658823 2.6971967764183006E-5 0.0 0.9239652137137352 0.0 68-69 0.0014564862592658823 2.6971967764183006E-5 0.0 1.0295469815266296 0.0 70-71 0.001469972243147974 2.6971967764183006E-5 0.0 1.1655261570097581 0.0 72-73 0.0015508881464405227 2.6971967764183006E-5 0.0 1.3160567091016635 0.0 74-75 0.0016452900336151634 2.6971967764183006E-5 0.0 1.4805991984470621 0.0 76-77 0.0016452900336151634 2.6971967764183006E-5 0.0 1.6685533558117713 0.0 78-79 0.0016452900336151634 2.6971967764183006E-5 0.0 1.886797032975658 0.0 80-81 0.0018745517596107189 2.6971967764183006E-5 0.0 2.1262541627860747 0.0 82-83 0.0018880377434928105 2.6971967764183006E-5 0.0 2.378010509896959 0.0 84-85 0.0019689536467853595 2.6971967764183006E-5 0.0 2.6349859327702125 0.0 86-87 0.0020228975823137255 2.6971967764183006E-5 0.0 2.9009834788605855 0.0 88-89 0.0020228975823137255 2.6971967764183006E-5 0.0 3.194829581667477 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11295 0.0 41.387688 1 GTATCAA 17955 0.0 34.44135 1 ATCAACG 22460 0.0 27.207369 3 TCAACGC 22770 0.0 26.864683 4 TATCAAC 22930 0.0 26.757843 2 CAACGCA 23260 0.0 26.253885 5 AACGCAG 23875 0.0 25.617323 6 GTACATG 15690 0.0 22.625584 1 TACATGG 15605 0.0 22.350138 2 ACGCAGA 27535 0.0 22.12613 7 CGCAGAG 27690 0.0 21.916662 8 GAGTACT 16250 0.0 21.351479 12-13 ACATGGG 15855 0.0 20.677225 3 GCAGAGT 29000 0.0 20.321724 9 GTACTTT 17035 0.0 20.131062 14-15 AGAGTAC 24835 0.0 19.88989 10-11 AGTACTT 17045 0.0 19.729935 12-13 CAGAGTA 28185 0.0 18.064049 10-11 CGTATAG 215 2.4144874E-5 17.659267 1 TACTTTT 19360 0.0 17.358458 14-15 >>END_MODULE