FastQCFastQC Report
Fri 27 May 2016
SRR939361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148631
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45950.2138571024992193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37560.17480898302221276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.11318835109425492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.051.339811
GTATCAA60050.043.0317041
ATCAACG76650.033.4612243
TATCAAC77550.033.2467082
TCAACGC78650.032.829634
CAACGCA79850.032.101455
AACGCAG82600.031.1017486
ACGCAGA93550.026.6989577
CGCAGAG93900.026.5942678
GCAGAGT102550.022.9956369
TGGTATC17350.022.7112052
GTGGTAT18400.022.4160161
GAGTACT65700.021.08095412-13
AGAGTAC89600.021.03358510-11
CAGAGTA97450.020.31622510-11
AGTACTT69150.020.20215612-13
GTACTTT69550.020.06462914-15
TACATGG56800.020.0411262
ACCGTCG1958.222238E-620.0096288
GTACATG59950.019.7281321
Produced by FastQC (version 0.11.5)