FastQCFastQC Report
Fri 27 May 2016
SRR939361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2148631
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61810.28767154527696936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.2671468483885786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.17974235687747223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.051.2129061
GTATCAA68050.036.0749471
ATCAACG87450.027.7894333
TATCAAC88800.027.691552
TCAACGC88150.027.5150174
CAACGCA90650.026.756195
AACGCAG94150.025.6609046
TATACCG1252.2983828E-422.7385835
GTACCGT1703.2257394E-622.2927276
ACGCAGA108700.022.0517527
CGCAGAG109100.021.797228
GTGGTAT22750.021.2482341
TGGTATC21600.021.0576732
GAGTACT73800.019.83694512-13
GCAGAGT118200.019.3175359
GTACTTT76800.018.81530614-15
CAGAGTA113400.018.79974410-11
AGAGTAC104900.018.65206710-11
TACATGG64650.018.6147862
GTACATG65850.018.4241831
Produced by FastQC (version 0.11.5)