##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2148631 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.875089766460597 31.0 31.0 31.0 28.0 34.0 2 31.16088197554629 31.0 31.0 34.0 28.0 34.0 3 31.14889387707801 31.0 31.0 34.0 28.0 34.0 4 35.090253282206206 35.0 35.0 37.0 32.0 37.0 5 34.699152623228464 35.0 35.0 37.0 32.0 37.0 6 34.653836326479514 35.0 35.0 37.0 30.0 37.0 7 34.52998211419271 35.0 35.0 37.0 30.0 37.0 8 34.539246152550156 35.0 35.0 37.0 30.0 37.0 9 35.89404788444363 37.0 35.0 39.0 30.0 39.0 10-11 35.75218080722097 37.0 35.0 39.0 30.0 39.0 12-13 35.709829887030395 37.0 35.0 39.0 30.0 39.0 14-15 36.57077971973783 38.0 35.0 40.0 30.0 41.0 16-17 36.563971431111256 38.0 35.0 40.0 30.0 41.0 18-19 36.500955259418674 38.0 35.0 40.0 30.0 41.0 20-21 36.38326799715726 38.0 34.5 40.0 30.0 41.0 22-23 36.23821540320325 38.0 34.0 40.0 29.0 41.0 24-25 36.024967293127574 38.0 34.0 40.0 29.0 41.0 26-27 35.62615940103257 38.0 34.0 40.0 27.0 41.0 28-29 35.339185509284746 38.0 34.0 40.0 27.0 41.0 30-31 35.128097844627575 38.0 33.5 40.0 27.0 41.0 32-33 34.91900633473128 37.0 33.0 40.0 26.0 41.0 34-35 34.91646332013268 37.5 33.0 40.0 26.0 41.0 36-37 34.93911658167457 38.0 33.0 40.0 25.5 41.0 38-39 34.90088409782787 38.0 33.0 40.0 26.0 41.0 40-41 34.685507655805026 38.0 33.0 40.0 25.0 41.0 42-43 34.400132223727574 37.5 33.0 40.0 24.5 41.0 44-45 34.22175585291285 37.0 33.0 40.0 24.0 41.0 46-47 33.93394794173592 37.0 32.5 40.0 24.0 41.0 48-49 33.65530377249514 37.0 32.0 40.0 23.0 41.0 50-51 33.387577718091194 37.0 31.5 40.0 22.5 41.0 52-53 33.02492610411001 36.5 31.0 39.0 20.5 40.5 54-55 32.78681425521646 36.0 31.0 39.0 19.5 40.0 56-57 32.369607903823415 36.0 30.5 39.0 18.5 40.0 58-59 31.917426258859713 35.5 30.0 39.0 16.5 40.0 60-61 31.603780267528485 35.0 29.5 38.5 14.5 40.0 62-63 31.215465568541084 35.0 29.0 38.0 13.0 40.0 64-65 30.765260763714195 34.0 28.5 38.0 8.5 40.0 66-67 28.92283574983327 33.0 25.0 37.5 7.0 39.5 68-69 25.035732752622483 29.0 15.5 35.0 4.0 38.0 70-71 24.36020377626498 28.5 12.0 34.5 2.0 38.0 72-73 26.3161585214027 31.0 21.0 35.0 2.0 38.0 74-75 26.989473529889498 31.0 22.5 35.0 2.0 38.0 76-77 27.197345193288193 31.5 25.0 34.5 2.0 36.5 78-79 28.06236203424413 32.5 26.0 35.0 2.0 37.0 80-81 27.92202546644817 33.0 26.0 35.0 2.0 37.0 82-83 27.49372577236389 32.0 26.0 35.0 2.0 36.0 84-85 27.122008618511042 32.0 25.5 35.0 2.0 36.0 86-87 26.644474551470218 32.0 25.0 34.0 2.0 35.5 88-89 26.121138529603268 32.0 24.0 34.0 2.0 35.0 90-91 25.615788378739765 31.0 22.5 34.0 2.0 35.0 92-93 24.98115474457922 31.0 19.5 34.0 2.0 35.0 94-95 23.47753313621557 30.0 7.0 34.0 2.0 35.0 96-97 22.626225489625718 29.5 2.0 34.0 2.0 35.0 98-99 22.246478106291868 30.0 2.0 34.0 2.0 35.0 100-101 20.940149332295775 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.324551847810838 1101 2 0.29684880119492973 1101 3 0.3542613842258042 1101 4 0.33171483114679745 1101 5 0.27938581136182705 1101 6 0.5339736781785547 1101 7 0.5535856897092586 1101 8 0.4497428227439144 1101 9 0.6364557394920851 1101 10-11 0.7541560279134814 1101 12-13 0.6090614798117073 1101 14-15 0.7488738380849895 1101 16-17 0.6851718919493663 1101 18-19 0.7166630779826662 1101 20-21 0.740121200098784 1101 22-23 0.7652881448301869 1101 24-25 0.7297169867607423 1101 26-27 0.7913272742010733 1101 28-29 0.6554286366856203 1101 30-31 0.7732699044629143 1101 32-33 0.744028989238366 1101 34-35 0.9418940714832615 1101 36-37 0.7287819327759593 1101 38-39 0.6479082335204183 1101 40-41 0.7524420082102239 1101 42-43 0.7827313810785341 1101 44-45 0.7503188700318404 1101 46-47 0.7815023904579732 1101 48-49 0.7312723695573098 1101 50-51 0.5196289249909896 1101 52-53 0.6987588974723806 1101 54-55 0.6130241398135254 1101 56-57 0.5517235843997668 1101 58-59 0.8094798500005318 1101 60-61 0.6783647682036111 1101 62-63 0.5182693948635553 1101 64-65 0.32777747300016813 1101 66-67 -2.8922084969713495 1101 68-69 -7.8874524137186235 1101 70-71 -11.164180088505518 1101 72-73 -11.493520643708887 1101 74-75 -9.949932631202032 1101 76-77 -4.928398003571981 1101 78-79 -1.8259402301783894 1101 80-81 -1.0229217910683914 1101 82-83 -0.8012591437414862 1101 84-85 -0.7758792331882383 1101 86-87 -0.579011549806129 1101 88-89 -0.6555175770297978 1101 90-91 -0.7122032205205393 1101 92-93 -1.5056508272065159 1101 94-95 -5.212022714184304 1101 96-97 -2.0256988532518783 1101 98-99 -1.2172589220960361 1101 100-101 -1.0403171895158394 1102 1 0.4658116657450222 1102 2 0.3406434720103846 1102 3 0.4539130797204578 1102 4 0.3363974720880947 1102 5 0.37561367895228415 1102 6 0.46412794782474265 1102 7 0.4797813826485964 1102 8 0.42850270797030987 1102 9 0.6154381376430749 1102 10-11 0.6336495777303597 1102 12-13 0.6991496056965971 1102 14-15 0.6374545286287301 1102 16-17 0.6666598035758398 1102 18-19 0.7698032916309643 1102 20-21 0.8317162229871116 1102 22-23 0.8878505964919441 1102 24-25 0.9222745574938287 1102 26-27 0.9801384630122598 1102 28-29 0.841941123429045 1102 30-31 0.6037455617284095 1102 32-33 0.4923363657212647 1102 34-35 0.6285380023535154 1102 36-37 0.8933230626750586 1102 38-39 0.8708437532226867 1102 40-41 0.8346403461822334 1102 42-43 0.8472359040126207 1102 44-45 0.6375555107326818 1102 46-47 0.8560540897209918 1102 48-49 0.6593733294134694 1102 50-51 0.6194676928436138 1102 52-53 0.950638144732693 1102 54-55 0.9296977151476185 1102 56-57 0.8064649938755863 1102 58-59 0.7859697445042819 1102 60-61 0.7019354366934571 1102 62-63 0.5960892527065091 1102 64-65 0.5753226529933855 1102 66-67 -2.567725884382501 1102 68-69 -7.596262400242239 1102 70-71 -11.007780419403772 1102 72-73 -9.311881010568097 1102 74-75 -7.946006803373205 1102 76-77 -4.014513197077477 1102 78-79 -0.9127965352794973 1102 80-81 -0.20931853520185584 1102 82-83 -0.014837008012190012 1102 84-85 0.06437712323440437 1102 86-87 0.06846337711452577 1102 88-89 0.19563114594340192 1102 90-91 0.05691853532177049 1102 92-93 -0.38559344040547927 1102 94-95 -4.338991908705463 1102 96-97 -1.0080552830678933 1102 98-99 -0.33330659010675134 1102 100-101 -0.2947777661473161 1103 1 0.41377559834207034 1103 2 0.2826242299727042 1103 3 0.3887645645143145 1103 4 0.20805518220143426 1103 5 0.5112264334663053 1103 6 0.4300697620185687 1103 7 0.5232338750061984 1103 8 0.4214479110576619 1103 9 0.7270162551758617 1103 10-11 0.7142248269571638 1103 12-13 0.6088624437613319 1103 14-15 0.7506100801246518 1103 16-17 0.7469875946220341 1103 18-19 0.6996168084594672 1103 20-21 0.8304604249583605 1103 22-23 0.8245406298973066 1103 24-25 0.835192784952973 1103 26-27 0.824270032556818 1103 28-29 0.7813664055358558 1103 30-31 0.8390878435555109 1103 32-33 0.7449779594116492 1103 34-35 0.9394672660296095 1103 36-37 0.9645092291359063 1103 38-39 1.002368149345621 1103 40-41 0.8295582745423431 1103 42-43 0.8665581641147497 1103 44-45 0.7003727251057867 1103 46-47 0.8884226048017609 1103 48-49 0.870676272852414 1103 50-51 0.8095440078719065 1103 52-53 0.9390315650316055 1103 54-55 0.9831485329088423 1103 56-57 0.9675175090387427 1103 58-59 1.0107472187055002 1103 60-61 0.882954752461135 1103 62-63 0.946556602405515 1103 64-65 0.47894269155387903 1103 66-67 -2.989349475714125 1103 68-69 -7.554345029185413 1103 70-71 -11.294396245205153 1103 72-73 -9.270936159116143 1103 74-75 -7.623786924391894 1103 76-77 -3.733473836898664 1103 78-79 -0.7282510150635986 1103 80-81 -0.10033365403103289 1103 82-83 0.17920005579052756 1103 84-85 0.11858389737971109 1103 86-87 0.3571358072583344 1103 88-89 0.4446987748612017 1103 90-91 0.4155324530981943 1103 92-93 0.49469911247638976 1103 94-95 1.2591258569113428 1103 96-97 1.5082591959818892 1103 98-99 1.1758057066544119 1103 100-101 1.2296902957775266 1104 1 0.3851492534559853 1104 2 0.2330100437782967 1104 3 0.3510400436059875 1104 4 0.14197697420166833 1104 5 0.36537342688453833 1104 6 0.5613271033145608 1104 7 0.5012296949594557 1104 8 0.5072022675678625 1104 9 0.6737923037168159 1104 10-11 0.7476191275014088 1104 12-13 0.6384090465314571 1104 14-15 0.6791891713873994 1104 16-17 0.6682384402470376 1104 18-19 0.7877079723950402 1104 20-21 0.8059717604565151 1104 22-23 0.6805446317027162 1104 24-25 0.7898858202409755 1104 26-27 0.9160811142956007 1104 28-29 0.9013408283584923 1104 30-31 1.0083528794905092 1104 32-33 0.7593310772807627 1104 34-35 0.8747969073363322 1104 36-37 0.8634183256021402 1104 38-39 0.8045533305911832 1104 40-41 0.940288811322624 1104 42-43 1.1358731015706525 1104 44-45 0.9276783894731224 1104 46-47 0.7282148159581254 1104 48-49 0.7079771150200074 1104 50-51 0.6521386781534346 1104 52-53 0.7918384196667247 1104 54-55 0.7656752687305257 1104 56-57 0.5659115667329502 1104 58-59 0.6827339907171286 1104 60-61 0.4809787097304259 1104 62-63 0.5887645132037456 1104 64-65 0.3536931272086825 1104 66-67 -2.9738453546020125 1104 68-69 -7.312630262829082 1104 70-71 -8.907487950068372 1104 72-73 -8.264167149443448 1104 74-75 -7.282581643788138 1104 76-77 -3.7751383537246888 1104 78-79 -0.8143464207797599 1104 80-81 0.061259514816917715 1104 82-83 0.24316917555523432 1104 84-85 0.3443677771911844 1104 86-87 0.45921588966695737 1104 88-89 0.3461817790659154 1104 90-91 0.3006946489285234 1104 92-93 0.36817802772203123 1104 94-95 1.3070332839916468 1104 96-97 1.366449604631164 1104 98-99 1.2196306809281978 1104 100-101 1.332747830657521 1105 1 0.4342442206467503 1105 2 0.4667972310704691 1105 3 0.5567291320961587 1105 4 0.3428122387110335 1105 5 0.5697690032116896 1105 6 0.5680961262524846 1105 7 0.6685238295976106 1105 8 0.5670499093677961 1105 9 0.7995540395331986 1105 10-11 0.8791833785083583 1105 12-13 0.8387899893321773 1105 14-15 1.0347470324064219 1105 16-17 1.061823020396389 1105 18-19 0.9946569896632056 1105 20-21 0.965077146380068 1105 22-23 1.0406346730150133 1105 24-25 1.1085920652685033 1105 26-27 1.1147860412146855 1105 28-29 1.308623093713905 1105 30-31 1.1363676189701621 1105 32-33 1.2955088182131718 1105 34-35 1.2668898648847247 1105 36-37 1.2221914409829893 1105 38-39 1.1023985386205482 1105 40-41 1.1931254248434584 1105 42-43 1.288527087697716 1105 44-45 1.1176912899076896 1105 46-47 1.046207611139522 1105 48-49 0.9414936643760754 1105 50-51 1.024641248937904 1105 52-53 1.3566471788442058 1105 54-55 1.2130337857134705 1105 56-57 1.2019510797139574 1105 58-59 1.29327520036858 1105 60-61 1.321897762420388 1105 62-63 1.6046857180683851 1105 64-65 1.5935255603075795 1105 66-67 2.264180355450076 1105 68-69 -4.430515039338751 1105 70-71 -5.131732883628452 1105 72-73 -1.406150054396793 1105 74-75 1.2918025583352772 1105 76-77 1.5016451841729754 1105 78-79 1.3379106349633005 1105 80-81 1.169656906223544 1105 82-83 1.4164326386297539 1105 84-85 1.39315576386252 1105 86-87 1.4246588229817085 1105 88-89 1.3395731838005744 1105 90-91 1.4271405870890597 1105 92-93 1.6253668429727384 1105 94-95 2.4322948954076047 1105 96-97 2.6362883192420803 1105 98-99 2.335805700502739 1105 100-101 2.270295426828195 1106 1 0.12210371716891899 1106 2 0.12074594785322645 1106 3 0.23750953735342506 1106 4 0.05673282751472897 1106 5 0.27865627530116654 1106 6 0.16167606119435618 1106 7 0.2623701033711896 1106 8 0.20987833031956882 1106 9 0.3485888048708219 1106 10-11 0.47449207983046904 1106 12-13 0.2817110604690072 1106 14-15 0.2539784824751976 1106 16-17 0.2875220380283423 1106 18-19 0.30160437312779464 1106 20-21 0.5721415402886976 1106 22-23 0.3427606740186562 1106 24-25 0.49112746478487423 1106 26-27 0.5837207833288645 1106 28-29 0.6981442847032113 1106 30-31 0.4488395031712358 1106 32-33 0.5250811170258487 1106 34-35 0.6780934728014785 1106 36-37 0.5206233711112702 1106 38-39 0.6661501484276613 1106 40-41 0.5346672181157999 1106 42-43 0.7059160901775883 1106 44-45 0.6623517647378634 1106 46-47 0.4881822050134019 1106 48-49 0.47634401930505277 1106 50-51 0.45286934865906403 1106 52-53 0.7303485740374427 1106 54-55 0.6855844022716227 1106 56-57 0.8604910557109804 1106 58-59 0.8752760546016667 1106 60-61 0.6333466781452621 1106 62-63 0.6113644890770793 1106 64-65 0.5943699680853278 1106 66-67 1.3169281926650847 1106 68-69 -5.4439589790747185 1106 70-71 -6.74497495801819 1106 72-73 -2.89615966857901 1106 74-75 0.1437535714784488 1106 76-77 0.28441728420594714 1106 78-79 0.24140998616839937 1106 80-81 0.2304177258325808 1106 82-83 0.3921287330306278 1106 84-85 0.31732633764406515 1106 86-87 0.5322437144242755 1106 88-89 0.5179265764538457 1106 90-91 0.4044326844586372 1106 92-93 0.4385967372329702 1106 94-95 1.364272282591184 1106 96-97 1.2784193871795928 1106 98-99 0.9933227080235625 1106 100-101 0.8706100801735914 1107 1 0.025354785305228944 1107 2 -0.11572483100464837 1107 3 0.06356279210404381 1107 4 -0.041226731038030096 1107 5 0.05139326032776381 1107 6 0.056716668136658654 1107 7 -0.03620637917427416 1107 8 0.0029658356158392962 1107 9 0.10964166537928577 1107 10-11 0.22244440338977256 1107 12-13 0.2174894461210144 1107 14-15 0.1896687485063424 1107 16-17 -0.06898551365853933 1107 18-19 0.1088424858602437 1107 20-21 0.2361210639575262 1107 22-23 0.07787300703976996 1107 24-25 0.23631671401783194 1107 26-27 0.2113400892373818 1107 28-29 0.2216639920455563 1107 30-31 0.04385576241101319 1107 32-33 0.10243706050798806 1107 34-35 0.251587576065198 1107 36-37 0.22000575394552868 1107 38-39 0.19046232583143308 1107 40-41 0.2934889279483741 1107 42-43 0.366739337121345 1107 44-45 0.29863348065303086 1107 46-47 0.08291010427457479 1107 48-49 0.2937099098201017 1107 50-51 0.10995076096443768 1107 52-53 0.43011757412669027 1107 54-55 0.22806169868886883 1107 56-57 0.38836796204510904 1107 58-59 0.3138341507326352 1107 60-61 0.27693235823031515 1107 62-63 0.3100915634623469 1107 64-65 0.4908148569307009 1107 66-67 1.1801535440821453 1107 68-69 -5.5793943307257265 1107 70-71 -6.289140997218858 1107 72-73 -2.645042663571701 1107 74-75 0.042547537060674046 1107 76-77 0.1519851913346244 1107 78-79 0.23696007528445762 1107 80-81 0.25207106480371877 1107 82-83 0.46796160208244686 1107 84-85 0.4824808762377728 1107 86-87 0.6456844260357002 1107 88-89 0.6279652815026537 1107 90-91 0.7157368635121593 1107 92-93 0.7625593821386261 1107 94-95 1.579841381977463 1107 96-97 1.6572186901941173 1107 98-99 1.364942038193135 1107 100-101 1.4045202778752035 1108 1 0.0970278762628709 1108 2 0.022374212638897717 1108 3 -0.007657252905829637 1108 4 0.07902830453802778 1108 5 0.059248512776434836 1108 6 0.11008461818191506 1108 7 0.15907798937473672 1108 8 0.11638277069260994 1108 9 0.2786001287394413 1108 10-11 0.12365055511038747 1108 12-13 0.18562841926839013 1108 14-15 0.17160873043122393 1108 16-17 -0.03538229960214778 1108 18-19 0.18297255587301464 1108 20-21 0.22171030313685236 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1.6468181877245058 2312 90-91 1.8358333498801507 2312 92-93 1.8016102799665568 2312 94-95 2.4567022760976336 2312 96-97 2.690815373228503 2312 98-99 2.440899676673773 2312 100-101 2.0229393901837014 2313 1 -0.22061825472745156 2313 2 -0.1855187562795031 2313 3 -0.18229037563193273 2313 4 -0.22951221084286288 2313 5 -0.20575492369291482 2313 6 -0.06041772565710346 2313 7 -3.408466111981877 2313 8 -1.3862811280901184 2313 9 -0.9350485936947166 2313 10-11 -0.13805249184405 2313 12-13 -1.5660920531620874 2313 14-15 -0.5245818568119134 2313 16-17 -0.07647151402355945 2313 18-19 0.1811905753586771 2313 20-21 -0.08350490499996965 2313 22-23 -1.2750446674671494 2313 24-25 -2.622664705530539 2313 26-27 -1.975061454953611 2313 28-29 -0.5281199062064488 2313 30-31 -0.16835148696439006 2313 32-33 -2.620764050570642 2313 34-35 -2.8764749707445603 2313 36-37 -1.2987411187508258 2313 38-39 -0.6843193923659925 2313 40-41 -0.8521810906392062 2313 42-43 -0.6419677394463434 2313 44-45 -0.21208121124446677 2313 46-47 -0.40074489048224393 2313 48-49 -0.49936839847719483 2313 50-51 -0.2157888179448122 2313 52-53 0.24192642006352116 2313 54-55 0.003578469879435886 2313 56-57 0.2243340234467439 2313 58-59 -0.07185508672480623 2313 60-61 0.335466639373589 2313 62-63 0.2769990719142008 2313 64-65 0.49397047057737353 2313 66-67 1.6054906664936226 2313 68-69 4.666262325048443 2313 70-71 4.50239051629908 2313 72-73 2.9632604826585016 2313 74-75 1.8912060604354082 2313 76-77 1.0765527237756771 2313 78-79 0.8579771769835745 2313 80-81 0.8564508497164667 2313 82-83 0.48175182900217806 2313 84-85 0.5710457469243018 2313 86-87 0.9393370405413819 2313 88-89 0.8848552979335125 2313 90-91 0.9084950783876771 2313 92-93 0.9945841259422963 2313 94-95 1.4900625586468799 2313 96-97 1.432217529545344 2313 98-99 1.116097098658642 2313 100-101 0.7279373310358643 2314 1 -0.3559690310356771 2314 2 -0.25305757169065757 2314 3 -0.18270728189990493 2314 4 -0.21509300440237666 2314 5 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66-67 -0.6002885169451346 2314 68-69 1.013049271569436 2314 70-71 1.1307617066383528 2314 72-73 -0.7096190458061322 2314 74-75 -0.10476144962785128 2314 76-77 -0.22790386046814248 2314 78-79 0.16552461804165475 2314 80-81 0.3747731613898395 2314 82-83 -0.09987554305072521 2314 84-85 -0.09844102708374791 2314 86-87 -0.413265029613882 2314 88-89 -0.34808748527522937 2314 90-91 0.1825965734998114 2314 92-93 0.009298041985999816 2314 94-95 -0.14727930449285864 2314 96-97 -0.04747625998887273 2314 98-99 -0.3374753567755171 2314 100-101 0.018147221777670808 2315 1 -0.39722095149095793 2315 2 -0.3342436015956629 2315 3 -0.3507968887204953 2315 4 -0.15038231693348791 2315 5 -1.3345544555553204 2315 6 -0.39667569120148016 2315 7 -0.39872997344482286 2315 8 -0.11549612746166815 2315 9 -0.10677102051484155 2315 10-11 -1.723698927336315 2315 12-13 -1.2518825486982479 2315 14-15 -0.14579740314201928 2315 16-17 -1.7061913911328404 2315 18-19 -3.1662717533311877 2315 20-21 -2.1155873852452345 2315 22-23 -1.8008385055278282 2315 24-25 -1.1017762293672035 2315 26-27 -0.4466631181127738 2315 28-29 -0.6153160576071528 2315 30-31 -3.4831279775070207 2315 32-33 -2.651448747839673 2315 34-35 -1.5489628172609997 2315 36-37 -4.905710880874498 2315 38-39 -2.2182579865996317 2315 40-41 -1.6237280579427704 2315 42-43 -1.7851318394439986 2315 44-45 -1.7077690783869386 2315 46-47 -1.3959461118388603 2315 48-49 -5.145976311604706 2315 50-51 -4.096055308307889 2315 52-53 -2.7572439340461017 2315 54-55 -4.71430808778533 2315 56-57 -7.182756686499019 2315 58-59 -7.664290852473691 2315 60-61 -6.631064607475526 2315 62-63 -7.364889942460035 2315 64-65 -6.60443280638577 2315 66-67 -4.533457755038313 2315 68-69 -2.508977809554924 2315 70-71 -0.17216522950515412 2315 72-73 -1.8635159471109617 2315 74-75 -3.5456674176455074 2315 76-77 -5.737746329037591 2315 78-79 -5.006164625244864 2315 80-81 -4.59135190082209 2315 82-83 -7.746898025901874 2315 84-85 -5.794594436297604 2315 86-87 -5.921017718251278 2315 88-89 -4.703461243741458 2315 90-91 -4.252002727562662 2315 92-93 -5.063584317956007 2315 94-95 -3.0881214376588524 2315 96-97 -1.941373810461684 2315 98-99 -3.0645145557022246 2315 100-101 -2.306766222491259 2316 1 -0.4954159279760546 2316 2 -0.5107218823958029 2316 3 -1.219786085290611 2316 4 -0.5024923281396667 2316 5 -0.4680005695272982 2316 6 -2.5905510347430365 2316 7 -0.7464395920519564 2316 8 -0.6960530143265018 2316 9 -0.20088488675988003 2316 10-11 -0.37425308047637884 2316 12-13 -0.9672760624666878 2316 14-15 -1.2847711364292564 2316 16-17 -1.7498200386207685 2316 18-19 -0.6308654960925608 2316 20-21 -4.429538980926647 2316 22-23 -4.500552751294549 2316 24-25 -3.3052818661963883 2316 26-27 -2.612017643262 2316 28-29 -1.8034366670399677 2316 30-31 -0.7268979717822006 2316 32-33 -2.434395870090036 2316 34-35 -2.788833601812179 2316 36-37 -5.110406001001877 2316 38-39 -2.740676616551376 2316 40-41 -1.5894483318160866 2316 42-43 -2.8447256482877705 2316 44-45 -0.7914356641316473 2316 46-47 -1.2410642567457515 2316 48-49 -0.9494430641553109 2316 50-51 -0.6424121157347145 2316 52-53 -2.9438742113348297 2316 54-55 -1.1905730267564891 2316 56-57 -0.28804439190226105 2316 58-59 -0.14513286752795906 2316 60-61 -1.6188661432306297 2316 62-63 -1.2699716098791143 2316 64-65 -0.3449407644464024 2316 66-67 0.7885871963006927 2316 68-69 3.4274700455145357 2316 70-71 3.8907274825793685 2316 72-73 2.0986449197232524 2316 74-75 0.9480384071359502 2316 76-77 -0.2864111514254759 2316 78-79 -0.116632495631535 2316 80-81 -0.628924269751117 2316 82-83 -0.36855260412570345 2316 84-85 -0.28813888088889783 2316 86-87 -0.48637947768488843 2316 88-89 0.04839236773155875 2316 90-91 0.3466756938886597 2316 92-93 0.43751821480869424 2316 94-95 0.6369068908249638 2316 96-97 0.5800553224189038 2316 98-99 -0.35288704344046096 2316 100-101 -0.6046549244171793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 26.0 4 78.0 5 154.0 6 360.0 7 965.0 8 2079.0 9 3527.0 10 5482.0 11 7946.0 12 10672.0 13 13359.0 14 16418.0 15 20098.0 16 23336.0 17 26424.0 18 25471.0 19 23687.0 20 23888.0 21 23881.0 22 25638.0 23 28633.0 24 34633.0 25 43333.0 26 54264.0 27 67279.0 28 82154.0 29 97132.0 30 113692.0 31 132612.0 32 151127.0 33 173399.0 34 199138.0 35 225052.0 36 233095.0 37 185795.0 38 70297.0 39 3500.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.517170074208835 21.06798154857697 16.279903376290136 23.134945000924063 2 15.949132218247902 21.21945633533746 39.21766509824805 23.61374634816659 3 19.222472355653437 24.340335776594493 30.671809165929375 25.7653827018227 4 13.643990056924618 18.031853771075628 36.86901101212819 31.455145159871567 5 13.544510052233358 37.5174937297167 34.81386020002541 14.124136018024533 6 31.76860056473168 37.18772557968306 17.051229364185847 13.992444491399409 7 27.745387644504802 32.769284255882006 20.89186091050534 18.593467189107855 8 25.54370666717552 36.30735105283318 19.34985579189726 18.799086488094048 9 25.148152474761837 17.237673662904427 20.577893551754585 37.03628031057916 10-11 24.580349068779146 27.134417217288593 27.473470316680714 20.81176339725155 12-13 25.277793162250756 24.28474223819725 28.01551313371165 22.421951465840344 14-15 22.22927063790851 25.9591107081672 26.309682770098732 25.50193588382556 16-17 21.7906952986412 28.89810615959474 27.06360607151509 22.247592470248975 18-19 21.583844788739867 27.54919815489757 29.375195184601 21.491761871761565 20-21 22.346047866303675 26.693165716487783 29.603554450148593 21.35723196705995 22-23 21.91453534832179 26.644337720157623 29.42936688523995 22.01176004628063 24-25 21.816565990158384 26.8243825952432 29.549722590803167 21.809328823795244 26-27 21.770483181666396 27.069250182384298 29.35760004374896 21.80266659220035 28-29 21.649803179798912 27.221445244231372 29.433982201113867 21.694769374855845 30-31 22.04856691122022 26.870117907919838 29.3173600131438 21.76395516771614 32-33 21.87480218352156 26.931363732838836 29.220542566382555 21.973291517257042 34-35 22.160355440370857 27.06718639417976 29.237255889000057 21.53520227644933 36-37 22.20796077569017 26.831092086186143 29.24962635527743 21.71132078284626 38-39 21.880360795203064 27.17080466089258 29.13106780284853 21.81776674105583 40-41 22.225566831704505 26.97472849834826 29.097791851024674 21.70191281892256 42-43 22.054560482157633 26.948235890478355 29.02145128105735 21.975752346306663 44-45 22.136497355071697 27.201193216851976 28.926470550553667 21.735838877522664 46-47 22.247188655621233 27.291943045162043 28.43311300701744 22.027755292199284 48-49 22.104090559877303 27.353042167257353 28.47208859936796 22.07077867349739 50-51 22.212301314606968 27.527521394917397 28.266332653286565 21.99384463718907 52-53 22.465288338866117 27.46349272742432 28.054321088862505 22.016897844847062 54-55 22.289694326296463 27.39277900744827 28.277466446684375 22.040060219570893 56-57 22.233500009195534 27.553873613307502 28.11873554176717 22.0938908357298 58-59 22.293201023696014 27.475894826313347 28.151547227707198 22.07935692228345 60-61 22.291407078002507 27.532580753117042 28.239468709956245 21.93654345892421 62-63 22.203535531241915 27.449445868635586 28.339937670693498 22.007080929429 64-65 22.083956726561286 27.412918685785844 28.291568605137435 22.211555982515435 66-67 21.859088431035726 27.338185334214838 28.748736425634497 22.053989809114942 68-69 22.134691220626713 26.783171919113624 29.325478156908382 21.756658703351285 70-71 21.807673590770808 26.598715655872468 29.65352691781612 21.9400838355406 72-73 22.236475595222256 27.179050529296518 28.361559213195136 22.22291466228609 74-75 21.86016188758679 27.46898149086967 28.15483719988131 22.516019421662225 76-77 21.928450569169648 27.53375335438869 27.945566388385263 22.5922296880564 78-79 22.1733029950343 27.511363480330726 27.914679468516567 22.400654056118405 80-81 22.056597038154738 27.572415995025583 27.785578748511234 22.585408218308448 82-83 22.148847335815226 27.624310549368413 27.846987221165477 22.37985489365089 84-85 22.1441233976425 27.580329056036145 27.669059973536637 22.606487572784715 86-87 21.976594793552806 27.552254942638882 27.834132953431368 22.63701731037694 88-89 22.033136679611435 27.545755034868957 27.687893043708133 22.73321524181147 90-91 21.948883283567294 27.586746152702275 27.766122649494186 22.69824791423625 92-93 21.9824350331263 27.463179426555495 27.748691586827672 22.805693953490536 94-95 21.600827827777856 27.449414282921715 28.11304346609027 22.836714423210154 96-97 21.428896354369208 27.328642382367946 28.24207378508328 23.000387478179565 98-99 21.69811869668665 27.323013818443165 27.729446831705356 23.249420653164826 100-101 21.647424558049 27.258708752073872 27.826185915330377 23.26768077454675 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 616.0 1 429.5 2 262.0 3 275.5 4 261.5 5 278.0 6 426.5 7 820.5 8 1305.0 9 1639.5 10 1658.0 11 1588.5 12 1631.0 13 1691.0 14 1860.0 15 2070.5 16 2288.0 17 2576.5 18 2932.5 19 3297.5 20 3684.5 21 4349.5 22 5354.5 23 6696.5 24 8551.0 25 10953.5 26 14170.0 27 18145.5 28 21662.0 29 25029.5 30 28857.5 31 33100.5 32 37874.5 33 42338.5 34 46585.0 35 50624.5 36 55016.0 37 59545.0 38 62584.0 39 64887.5 40 67590.5 41 70560.0 42 73101.5 43 75195.0 44 77055.0 45 78445.5 46 79257.0 47 79468.5 48 79816.0 49 79378.0 50 77435.0 51 74824.5 52 72145.0 53 69723.5 54 66797.0 55 62410.0 56 56655.5 57 50619.0 58 45377.0 59 39781.5 60 33219.0 61 27049.0 62 21640.0 63 16605.0 64 12215.0 65 9025.5 66 6646.0 67 4941.5 68 3717.0 69 2766.5 70 2053.0 71 1467.5 72 1080.5 73 852.5 74 666.0 75 494.0 76 341.5 77 229.0 78 150.0 79 83.5 80 49.5 81 37.0 82 23.5 83 11.5 84 7.0 85 5.0 86 4.0 87 3.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023456796443875192 2 0.018383798800259327 3 0.0 4 0.0 5 1.861650511418666E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.490594708909999E-4 18-19 0.0011169903068511996 20-21 2.3270631392733326E-5 22-23 0.0 24-25 0.0 26-27 3.956007336764665E-4 28-29 0.01631271260630606 30-31 0.004188713650691999 32-33 0.008144720987456664 34-35 0.009191899400129664 36-37 0.1027165669675249 38-39 0.10522979515794009 40-41 0.11902927957383096 42-43 0.10606753788807852 44-45 0.10073856329914258 46-47 0.1020882599199211 48-49 0.10471784126729997 50-51 0.10285619075588129 52-53 0.08051638461885731 54-55 0.0692766696561671 56-57 0.03953680273625392 58-59 0.015684405558702263 60-61 0.009308252557093331 62-63 0.002001274299775066 64-65 0.005003185749437666 66-67 0.45875722727634477 68-69 0.8682505278942731 70-71 0.9668714637366771 72-73 0.300098062440689 74-75 0.16291769038052603 76-77 0.01517245166806213 78-79 0.008912651823416865 80-81 0.001768567985847733 82-83 0.0 84-85 0.0 86-87 3.0251820810553323E-4 88-89 6.981189417819998E-4 90-91 0.0036069478658736654 92-93 0.0016754854602767993 94-95 0.24154915385657194 96-97 0.24594730318979854 98-99 0.01426489704374553 100-101 0.00812145035606393 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2148631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.14331373052215 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39371031791326 49.50038645103669 2 12.83326067125167 16.206692095008314 3 4.078716409753206 7.726310096366269 4 1.7479720534108414 4.414909910428231 5 0.8580586676384618 2.7090333824944617 6 0.5247578994471535 1.9880971606416848 7 0.3230327049498245 1.4278148803706177 8 0.20826425092053913 1.0520395947782233 9 0.16311071660332388 0.9269416036164574 >10 0.8032250557237757 8.843601925622211 >50 0.044968789308997635 1.9417675077932435 >100 0.01995906926085918 2.1819844431010424 >500 5.928577341566433E-4 0.2224107050705634 >1k 2.2232165030866379E-4 0.3001819215289775 >5k 1.482144335391092E-4 0.557828322142937 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6181 0.28767154527696936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5740 0.2671468483885786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3862 0.17974235687747223 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 4.654126278546665E-5 0.0 0.0 0.0 20-21 0.0 4.654126278546665E-5 0.0 0.0 0.0 22-23 0.0 4.654126278546665E-5 0.0 2.3270631392733326E-5 2.3270631392733326E-5 24-25 0.0 4.654126278546665E-5 0.0 1.6289441974913328E-4 4.654126278546665E-5 26-27 0.0 4.654126278546665E-5 0.0 3.2578883949826656E-4 4.654126278546665E-5 28-29 0.0 6.981189417819998E-5 0.0 0.001210072832422133 9.30825255709333E-5 30-31 0.0 9.30825255709333E-5 0.0 0.003723301022837332 9.30825255709333E-5 32-33 0.0 9.30825255709333E-5 0.0 0.008656674878096796 9.30825255709333E-5 34-35 4.654126278546665E-5 9.30825255709333E-5 0.0 0.017452973544549995 9.30825255709333E-5 36-37 4.654126278546665E-5 9.30825255709333E-5 0.0 0.031997118165008324 9.30825255709333E-5 38-39 4.654126278546665E-5 9.30825255709333E-5 0.0 0.05238219126504272 9.30825255709333E-5 40-41 4.654126278546665E-5 9.30825255709333E-5 0.0 0.07604842339145251 9.30825255709333E-5 42-43 4.654126278546665E-5 9.30825255709333E-5 0.0 0.1000637149887533 9.30825255709333E-5 44-45 4.654126278546665E-5 9.30825255709333E-5 0.0 0.13143252610615783 9.30825255709333E-5 46-47 4.654126278546665E-5 9.30825255709333E-5 0.0 0.169898879798346 9.30825255709333E-5 48-49 4.654126278546665E-5 9.30825255709333E-5 0.0 0.21132060367741134 9.30825255709333E-5 50-51 4.654126278546665E-5 9.30825255709333E-5 0.0 0.2558373215317102 9.30825255709333E-5 52-53 4.654126278546665E-5 9.30825255709333E-5 0.0 0.3012848646417184 9.30825255709333E-5 54-55 4.654126278546665E-5 9.30825255709333E-5 0.0 0.35524945884146697 9.30825255709333E-5 56-57 4.654126278546665E-5 1.1635315696366662E-4 0.0 0.41775437476234867 9.30825255709333E-5 58-59 4.654126278546665E-5 1.861650511418666E-4 0.0 0.47444163283504703 9.30825255709333E-5 60-61 4.654126278546665E-5 2.3270631392733327E-4 0.0 0.5342704261457645 9.30825255709333E-5 62-63 4.654126278546665E-5 2.3270631392733327E-4 0.0 0.5950765859749766 9.30825255709333E-5 64-65 4.654126278546665E-5 2.3270631392733327E-4 0.0 0.6626544995394742 9.30825255709333E-5 66-67 4.654126278546665E-5 2.792475767127999E-4 0.0 0.7299764361586517 9.30825255709333E-5 68-69 4.654126278546665E-5 2.792475767127999E-4 0.0 0.8155890890525177 9.30825255709333E-5 70-71 4.654126278546665E-5 2.792475767127999E-4 0.0 0.9314302921255442 9.30825255709333E-5 72-73 4.654126278546665E-5 2.792475767127999E-4 0.0 1.0620250755015634 9.30825255709333E-5 74-75 4.654126278546665E-5 2.792475767127999E-4 0.0 1.2029054779531712 9.30825255709333E-5 76-77 4.654126278546665E-5 2.792475767127999E-4 0.0 1.3660558746476243 9.30825255709333E-5 78-79 4.654126278546665E-5 2.792475767127999E-4 0.0 1.5535240811474842 9.30825255709333E-5 80-81 4.654126278546665E-5 2.792475767127999E-4 0.0 1.7597251459184942 9.30825255709333E-5 82-83 4.654126278546665E-5 2.792475767127999E-4 0.0 1.9747690506187427 9.30825255709333E-5 84-85 6.981189417819998E-5 2.792475767127999E-4 0.0 2.188672694380748 9.30825255709333E-5 86-87 9.30825255709333E-5 2.792475767127999E-4 0.0 2.413560076160122 9.30825255709333E-5 88-89 9.30825255709333E-5 2.792475767127999E-4 0.0 2.671259048203251 9.30825255709333E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4155 0.0 51.212906 1 GTATCAA 6805 0.0 36.074947 1 ATCAACG 8745 0.0 27.789433 3 TATCAAC 8880 0.0 27.69155 2 TCAACGC 8815 0.0 27.515017 4 CAACGCA 9065 0.0 26.75619 5 AACGCAG 9415 0.0 25.660904 6 TATACCG 125 2.2983828E-4 22.738583 5 GTACCGT 170 3.2257394E-6 22.292727 6 ACGCAGA 10870 0.0 22.051752 7 CGCAGAG 10910 0.0 21.79722 8 GTGGTAT 2275 0.0 21.248234 1 TGGTATC 2160 0.0 21.057673 2 GAGTACT 7380 0.0 19.836945 12-13 GCAGAGT 11820 0.0 19.317535 9 GTACTTT 7680 0.0 18.815306 14-15 CAGAGTA 11340 0.0 18.799744 10-11 AGAGTAC 10490 0.0 18.652067 10-11 TACATGG 6465 0.0 18.614786 2 GTACATG 6585 0.0 18.424183 1 >>END_MODULE