FastQCFastQC Report
Fri 27 May 2016
SRR939360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113104
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45570.21565431706153604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.16175256873301078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.11901922479915802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32550.046.0332761
GTATCAA59500.043.818261
ATCAACG77200.033.491313
TCAACGC77800.033.4421734
TATCAAC76950.033.391472
CAACGCA78400.032.896345
AACGCAG82000.031.1389456
ACGCAGA90450.027.5355037
CGCAGAG93250.026.813728
TACATGG56750.024.3997882
GTACATG59000.023.9571971
GCAGAGT97800.023.885499
TGGTATC18700.022.6835612
GTGGTAT21100.022.4875811
ACATGGG60000.022.0266593
AGAGTAC86150.021.58521810-11
AGTACTT64400.020.2632212-13
GAGTACT59850.020.20048712-13
CAGAGTA94200.019.25288210-11
GTGTAGC13400.019.0093361
Produced by FastQC (version 0.11.5)