FastQCFastQC Report
Fri 27 May 2016
SRR939360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113104
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.2846050170744081No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53950.25531161741211034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.18167586640316805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38150.050.255011
GTATCAA64750.038.258091
ATCAACG83150.029.2631683
TCAACGC83650.029.0370544
TATCAAC84650.028.7541582
CAACGCA85450.028.4045375
AACGCAG89400.027.3616316
TGGTATC19850.024.1418822
GTGGTAT21000.023.7280791
ACGCAGA105300.023.1400627
CGCAGAG105700.022.9179488
GTGTAGC14550.022.5049821
GCAGAGT115650.020.2903429
AGAGTAC100950.019.60350210-11
GAGTACT70850.019.5335112-13
GTACATG68150.019.4281251
TACATGG64300.019.333062
TAGCCCT16600.019.147644
AGTACTT76550.018.91486712-13
CAGAGTA110700.018.41214410-11
Produced by FastQC (version 0.11.5)