FastQCFastQC Report
Fri 27 May 2016
SRR939359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429241
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88200.6171107601867005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66780.46724100414135894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.3425594423893521No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41420.28980416878608994No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19250.13468687226297035No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.10628018647659843No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.10110261320519073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40550.048.8802151
GTATCAA71050.040.901841
TATCAAC94250.031.2472362
ATCAACG94100.031.052833
TCAACGC95300.030.793764
CAACGCA96400.030.329095
AACGCAG98800.029.6582576
GTACATG74450.027.0902121
TACATGG74900.025.7590052
ACGCAGA112200.025.7403457
CGCAGAG113400.025.3718418
ACATGGG75700.024.1964953
GCAGAGT115300.023.6657719
GAGTACT70850.022.42146512-13
AGTACAT55550.022.3088132
CATGGGA53050.021.7129554
GAGTACA59200.021.4817331
AGTACTT73350.021.23325712-13
AGAGTAC105900.020.74294910-11
TAGCCCT8850.020.5223734
Produced by FastQC (version 0.11.5)