FastQCFastQC Report
Fri 27 May 2016
SRR939359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429241
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121700.8515009015274541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113790.7961568412884881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87060.6091344986604779No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58200.40720914107557793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.22802312556104953No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.18352398230949155No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24430.17092988516282417No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15390.1076795306040059No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.10746962898489477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57850.046.7706341
GTATCAA92500.035.6027531
ATCAACG122500.026.60273
TATCAAC123500.026.5837122
TCAACGC123900.026.3021054
CAACGCA125900.025.884285
AACGCAG129100.025.3160676
GTACATG80500.022.426921
ACGCAGA150350.021.7064677
TACATGG81000.021.6404382
CGCAGAG151350.021.5004568
GAGTACT96400.021.25263612-13
GCACCGT2701.036824E-921.051886
ATACCGT1605.0995397E-520.7229426
AGTACTT98550.020.69284812-13
GCAGAGT154400.020.3701469
ACATGGG82150.019.9499553
GTACTTT103300.019.90183814-15
GAGTACA69000.019.6407241
ACCGTCG1708.1291815E-519.5039468
Produced by FastQC (version 0.11.5)