FastQCFastQC Report
Fri 27 May 2016
SRR939358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383978
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87690.6336083377047901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63610.4596171326422819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49060.3544854036697115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39530.2856259275797737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19700.1423433031449922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.10570977284320993No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.10108542187809344No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.10043512252362392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41700.047.3346021
GTATCAA70600.040.4156041
ATCAACG94050.030.0046023
TATCAAC94550.029.9607962
TCAACGC95750.029.8231334
GTACATG75700.029.507291
CAACGCA96550.029.0878075
TACATGG76650.028.624582
AACGCAG99850.028.1045996
ACATGGG79500.027.0301823
AACGCGG1351.1641989E-525.087756
ACGCAGA113650.024.393987
CATGGGG26300.024.289264
CAACGCG1601.5737078E-624.2105855
CGCAGAG115650.023.8893328
GTATAGG4550.023.063521
GCAGAGT117550.022.4733319
CATGGGA53450.022.208384
CTACACG2901.0186341E-1021.37034
GAGTACT70600.021.34554312-13
Produced by FastQC (version 0.11.5)