FastQCFastQC Report
Fri 27 May 2016
SRR939358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383978
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114760.8292039324324519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102740.7423528408688578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81740.5906163248259727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56140.40564228622131276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.23035048244986553No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.17767623473783542No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.16813851087228265No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.1083832257449179No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.10744390445512862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.046.507191
GTATCAA89850.036.7080041
TATCAAC121800.027.1080912
ATCAACG122000.026.9042193
TCAACGC123350.026.4616974
CAACGCA125450.025.9980475
AACGCAG128150.025.4502946
GTACATG76100.025.068911
TACATGG78100.024.1751422
ACATGGG78900.022.9646873
ACGCAGA147550.021.8791717
CGCAGAG148400.021.658028
TATAGCG1100.002911327421.5476685
GAACCGT1100.002911327421.5476686
AGTACTT97900.020.88944812-13
GAGTACT96000.020.78450412-13
GCAGAGT151650.020.662469
GTATAGC5550.020.521651
AGAGTAC136150.020.26195110-11
TAGCCCT9950.020.0260184
Produced by FastQC (version 0.11.5)