##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939358_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1383978 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.474806680453014 31.0 30.0 31.0 27.0 34.0 2 30.725561388981617 31.0 30.0 31.0 27.0 34.0 3 30.681167619716497 31.0 30.0 33.0 27.0 34.0 4 34.62933803861044 35.0 35.0 37.0 32.0 37.0 5 34.174624885655696 35.0 35.0 37.0 30.0 37.0 6 34.283482829929376 35.0 35.0 37.0 30.0 37.0 7 34.107062395500506 35.0 33.0 37.0 30.0 37.0 8 34.0165811884293 35.0 33.0 37.0 30.0 37.0 9 35.428862308504904 37.0 34.0 39.0 30.0 39.0 10-11 35.20198045055629 37.0 34.0 39.0 29.5 39.0 12-13 35.18160079134206 37.0 34.0 39.0 29.5 39.0 14-15 35.86017010386003 38.0 34.0 40.0 28.5 41.0 16-17 35.76173140035463 38.0 34.0 40.0 28.0 41.0 18-19 35.785581851734634 38.0 34.0 40.0 28.0 41.0 20-21 35.66590148109291 38.0 34.0 40.0 27.0 41.0 22-23 35.47334278435062 38.0 34.0 40.0 27.0 41.0 24-25 35.270704808891466 37.0 33.0 40.0 27.0 41.0 26-27 34.712655114459906 37.0 33.0 39.5 25.5 40.5 28-29 34.4251155003909 37.0 32.5 39.0 25.0 40.0 30-31 34.035507789863715 36.5 32.0 39.0 24.5 40.0 32-33 33.683199443921794 36.0 31.5 39.0 24.0 40.0 34-35 33.808129898018606 36.0 32.0 39.0 24.0 40.0 36-37 33.573392062590585 36.0 31.5 39.0 24.0 40.0 38-39 33.321935753313994 36.0 31.0 39.0 23.0 40.0 40-41 33.090635472529186 36.0 31.0 39.0 22.0 40.0 42-43 32.81845159388372 36.0 31.0 39.0 20.5 40.0 44-45 32.32749472896245 35.5 30.0 39.0 18.0 40.0 46-47 31.801720836602897 35.0 30.0 39.0 15.0 40.0 48-49 31.312851432609477 35.0 29.5 39.0 11.0 40.0 50-51 31.000612365225457 35.0 29.0 38.0 9.0 40.0 52-53 30.82865262309083 35.0 28.5 38.0 8.5 40.0 54-55 30.43098011673596 34.5 28.0 38.0 8.0 40.0 56-57 29.87557750195451 34.0 27.0 38.0 6.5 40.0 58-59 29.338274163317624 34.0 26.0 38.0 2.0 40.0 60-61 28.91275186455276 33.0 26.0 37.5 2.0 40.0 62-63 28.675136815758634 33.0 26.0 37.0 2.0 39.0 64-65 28.147791366625768 33.0 25.5 37.0 2.0 39.0 66-67 27.131167908738433 32.0 23.0 36.0 2.0 39.0 68-69 23.67052872227738 28.5 10.0 34.0 2.0 38.0 70-71 22.301498289712697 26.5 8.0 34.0 2.0 37.0 72-73 23.241503477656437 28.0 10.5 34.0 2.0 36.5 74-75 23.91072798845068 29.0 13.5 34.0 2.0 36.0 76-77 23.81643349822035 29.0 15.5 33.5 2.0 36.0 78-79 24.93407409655356 30.5 19.5 34.0 2.0 36.0 80-81 24.91137575886322 31.0 18.5 34.0 2.0 36.0 82-83 24.51907291878917 30.5 18.0 34.0 2.0 35.5 84-85 24.085553383074007 30.0 15.0 34.0 2.0 35.0 86-87 23.55618441911649 30.0 9.0 34.0 2.0 35.0 88-89 22.951045464595538 29.5 6.0 34.0 2.0 35.0 90-91 22.228199436696247 29.0 2.0 34.0 2.0 35.0 92-93 21.51881352160222 29.0 2.0 33.0 2.0 35.0 94-95 20.506880889725124 27.0 2.0 33.0 2.0 34.0 96-97 19.67327154044356 26.5 2.0 33.0 2.0 34.0 98-99 18.69577514960498 25.0 2.0 32.0 2.0 34.0 100-101 17.319654647689486 22.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2196345420874799 1101 2 0.2550645460272598 1101 3 0.37797922855836674 1101 4 0.522983665960183 1101 5 0.7097385282351425 1101 6 0.3967986603721414 1101 7 0.5660865851682857 1101 8 0.7379820929777381 1101 9 0.7797972302128358 1101 10-11 0.8535057425853338 1101 12-13 0.7159566785169247 1101 14-15 0.6527755234148671 1101 16-17 0.8345763381099331 1101 18-19 0.6709843805012667 1101 20-21 0.816415795162591 1101 22-23 0.7514724265275632 1101 24-25 0.9956643422132387 1101 26-27 0.8326182871126591 1101 28-29 0.9704436751366714 1101 30-31 0.5925093278084788 1101 32-33 1.182202625302807 1101 34-35 0.9357372815484553 1101 36-37 1.3083293213608016 1101 38-39 1.3073016193788973 1101 40-41 1.242464075703218 1101 42-43 0.8682198136436128 1101 44-45 1.0862033513571134 1101 46-47 1.0775325738743255 1101 48-49 1.1443318948586096 1101 50-51 0.7172219769548782 1101 52-53 0.6038378572419276 1101 54-55 0.892593993966571 1101 56-57 0.9466412541484068 1101 58-59 0.7592905977769 1101 60-61 0.621752812657693 1101 62-63 0.22932230073775983 1101 64-65 0.1347886991804934 1101 66-67 -0.046056372887342434 1101 68-69 -6.413502452650878 1101 70-71 -7.300093463967656 1101 72-73 -5.006816106388445 1101 74-75 -3.0333114455668984 1101 76-77 -0.2918804931131582 1101 78-79 0.07783096704131864 1101 80-81 0.0585236505252702 1101 82-83 -0.028569260736958313 1101 84-85 -0.17756178487924146 1101 86-87 -0.2755181506316866 1101 88-89 -0.46736033482330797 1101 90-91 -0.4713165197120972 1101 92-93 -0.305860591834513 1101 94-95 -0.432327954359117 1101 96-97 -0.9159848421462584 1101 98-99 -0.7000997009838912 1101 100-101 -0.5927110688729087 1102 1 0.45635815196014207 1102 2 0.3225554617825672 1102 3 0.3861887071879231 1102 4 0.39845743883820717 1102 5 0.5890944720273694 1102 6 0.504001911315811 1102 7 0.5372150756857792 1102 8 0.6920741443076892 1102 9 0.6751601242011986 1102 10-11 0.6722984103398844 1102 12-13 0.7256374347208165 1102 14-15 0.9402357412957656 1102 16-17 0.892999221595467 1102 18-19 0.7792973014515994 1102 20-21 0.7158444834011775 1102 22-23 0.8184456020129645 1102 24-25 0.7638770633278043 1102 26-27 0.7584002722888599 1102 28-29 0.6250964649073367 1102 30-31 0.6712974842795276 1102 32-33 0.8298528810009458 1102 34-35 0.7488836198312754 1102 36-37 0.7747956823410149 1102 38-39 0.7314524936163025 1102 40-41 0.5066670411672334 1102 42-43 0.66120141959005 1102 44-45 0.7026638970109502 1102 46-47 0.9376854288471357 1102 48-49 0.8137852189344059 1102 50-51 0.8004683740958534 1102 52-53 0.6759329254927167 1102 54-55 0.445526856904614 1102 56-57 0.6504187491877538 1102 58-59 0.5668341334248481 1102 60-61 0.6736193562039112 1102 62-63 0.5925803637758946 1102 64-65 0.4847345990229499 1102 66-67 0.21807751354814542 1102 68-69 -6.4634696753567304 1102 70-71 -6.9601217299783045 1102 72-73 -1.542088880440108 1102 74-75 0.013988833216327379 1102 76-77 0.692317326668114 1102 78-79 0.458442140221738 1102 80-81 0.14480141870873453 1102 82-83 0.5245086329165787 1102 84-85 0.12254905876601896 1102 86-87 -0.008105653671517388 1102 88-89 -0.197575008477763 1102 90-91 -0.31160633443227326 1102 92-93 0.010894773244633171 1102 94-95 0.26792439556140835 1102 96-97 -0.19769370223655258 1102 98-99 -0.3454597754200712 1102 100-101 -0.13362541440198328 1103 1 0.4330390963493471 1103 2 0.34531727637697784 1103 3 0.44171822219193047 1103 4 0.3407389102988887 1103 5 0.39148434029738155 1103 6 0.4519911425815124 1103 7 0.45787538907013214 1103 8 0.6885095926886748 1103 9 0.7255336099986138 1103 10-11 0.5319831937683972 1103 12-13 0.5178867563367078 1103 14-15 0.5811345455695047 1103 16-17 0.7139504161679753 1103 18-19 0.6221742768933822 1103 20-21 0.5901904226192016 1103 22-23 0.7313902970474899 1103 24-25 0.6271496454511194 1103 26-27 0.7206096537065605 1103 28-29 0.9236820783770057 1103 30-31 0.7372215417655852 1103 32-33 0.8923064083744592 1103 34-35 0.9511503991400687 1103 36-37 1.1564665168352732 1103 38-39 1.220230355236616 1103 40-41 1.0264243003679994 1103 42-43 0.7347227936403229 1103 44-45 0.8840845929698489 1103 46-47 1.038309965842874 1103 48-49 0.7868864580552071 1103 50-51 0.6641154924400006 1103 52-53 0.5449728488152488 1103 54-55 0.1777654883096602 1103 56-57 0.461320146801949 1103 58-59 0.6655100488688639 1103 60-61 0.5212449343242476 1103 62-63 0.05543239768838504 1103 64-65 0.13197933838190323 1103 66-67 0.22642149174617643 1103 68-69 -6.434100869626175 1103 70-71 -7.248977322931996 1103 72-73 -1.3382301069420492 1103 74-75 0.3407864090457018 1103 76-77 0.5119053815973373 1103 78-79 0.42173397436780036 1103 80-81 0.4560206985591009 1103 82-83 0.41433936320882836 1103 84-85 0.09369272190590294 1103 86-87 0.13381619413693713 1103 88-89 0.30932386260979783 1103 90-91 -0.04802892287534277 1103 92-93 0.025428672819327147 1103 94-95 0.19179022239445587 1103 96-97 0.21523101753286156 1103 98-99 0.16759457693189717 1103 100-101 0.28475255274284095 1104 1 0.37529821474216973 1104 2 0.17564481406261834 1104 3 0.40951615470607194 1104 4 0.2688847209929719 1104 5 0.5609753403252498 1104 6 0.4909567727188815 1104 7 0.5917531092629531 1104 8 0.6763446535708368 1104 9 0.6923221803126367 1104 10-11 0.6370707554168149 1104 12-13 0.4987250703047721 1104 14-15 0.5784723440592927 1104 16-17 0.5852250407047421 1104 18-19 0.5635795473105034 1104 20-21 0.7921607338566261 1104 22-23 0.6707535072233171 1104 24-25 0.4465661101060263 1104 26-27 0.7020668899070657 1104 28-29 0.5245087592541537 1104 30-31 0.4255165276944197 1104 32-33 0.5489940424684718 1104 34-35 0.2778833465612891 1104 36-37 0.8773412974087123 1104 38-39 0.739184282623846 1104 40-41 0.9135397814346149 1104 42-43 0.5974328162099098 1104 44-45 0.6580560744822961 1104 46-47 0.7570378490382801 1104 48-49 0.9069655561075365 1104 50-51 0.6534156610153268 1104 52-53 0.5207486784566413 1104 54-55 0.49314467831216646 1104 56-57 0.6548961728823635 1104 58-59 0.8370957018544587 1104 60-61 0.918247323635736 1104 62-63 0.8076834356108691 1104 64-65 0.7205867459441073 1104 66-67 0.501503863782176 1104 68-69 -6.196721728385125 1104 70-71 -4.405006851478348 1104 72-73 0.31166122466373736 1104 74-75 1.1128016853141993 1104 76-77 1.4700349616915176 1104 78-79 0.9606118338790743 1104 80-81 0.7550930118027104 1104 82-83 0.5635447255761292 1104 84-85 0.5201677560389264 1104 86-87 0.7656833748886989 1104 88-89 0.8879896794338933 1104 90-91 0.6260352054090923 1104 92-93 0.5158691629416516 1104 94-95 0.5238561060914115 1104 96-97 0.5717361444519717 1104 98-99 0.656659993912033 1104 100-101 0.9883001230289992 1105 1 0.08942788880735719 1105 2 0.2273666125600613 1105 3 0.17462313331034807 1105 4 0.024042975056445925 1105 5 0.39142817832907895 1105 6 0.16149230846887264 1105 7 0.25340756408627385 1105 8 0.4710146481433526 1105 9 0.6341988049877045 1105 10-11 0.5468970044527239 1105 12-13 0.5488209439871738 1105 14-15 0.46540227950077906 1105 16-17 0.47463215928102187 1105 18-19 0.5492279533435891 1105 20-21 0.5480442000205343 1105 22-23 0.4307359750154731 1105 24-25 0.3891529498809234 1105 26-27 0.41189117505003736 1105 28-29 0.5855013821581423 1105 30-31 0.6042156093066708 1105 32-33 0.688613618553255 1105 34-35 0.7346925204407952 1105 36-37 0.7727072242233461 1105 38-39 0.7505116600055075 1105 40-41 0.8027387273597171 1105 42-43 0.8221050246253654 1105 44-45 0.8394644356672032 1105 46-47 0.7962364378919382 1105 48-49 1.1570389342890408 1105 50-51 1.0135977317440386 1105 52-53 1.1423963132154071 1105 54-55 1.0698420174157732 1105 56-57 0.8565629823776106 1105 58-59 1.2149811961841621 1105 60-61 1.2419488333337974 1105 62-63 0.9283060967929195 1105 64-65 0.7517099855818934 1105 66-67 0.951051989871246 1105 68-69 -3.381653313496674 1105 70-71 -3.7694237895193083 1105 72-73 0.7393371180564863 1105 74-75 1.4318109191817108 1105 76-77 1.4323514269698912 1105 78-79 1.2588918207388424 1105 80-81 0.9296268550340656 1105 82-83 1.0288839791539814 1105 84-85 1.0407911313638571 1105 86-87 0.8804713101587893 1105 88-89 0.8944894509081713 1105 90-91 0.9042592034666193 1105 92-93 0.5807310120004097 1105 94-95 0.5926769847728153 1105 96-97 0.7833484648450373 1105 98-99 0.977400667205945 1105 100-101 0.8224481148592062 1106 1 0.09254681809412446 1106 2 0.27096050085875234 1106 3 0.23155215718900024 1106 4 0.30152416410727767 1106 5 0.47307851492842445 1106 6 0.31443043592717856 1106 7 0.3455362519427396 1106 8 0.29456204294744737 1106 9 0.34693111886012673 1106 10-11 0.5504043104403138 1106 12-13 0.30186586572909135 1106 14-15 0.5743106654213577 1106 16-17 0.29048574833975493 1106 18-19 0.41108165336708424 1106 20-21 0.4201502257427663 1106 22-23 0.34677716156400606 1106 24-25 0.6162488714787244 1106 26-27 0.6439624516699496 1106 28-29 0.49512206509790957 1106 30-31 0.5933055008255721 1106 32-33 0.6219999394268854 1106 34-35 0.6204351112150093 1106 36-37 0.7770774861113736 1106 38-39 0.874141496421835 1106 40-41 0.6322205032578054 1106 42-43 0.6624412404362161 1106 44-45 0.7541088810797945 1106 46-47 1.0107378579058839 1106 48-49 1.14087140429411 1106 50-51 1.0053338351751222 1106 52-53 0.5748371993398571 1106 54-55 0.5221977287688482 1106 56-57 0.8030517107027073 1106 58-59 1.1514978034772305 1106 60-61 1.087951699799607 1106 62-63 0.6330370314253564 1106 64-65 0.5680722252382466 1106 66-67 0.7252241572999552 1106 68-69 -2.6907715643344012 1106 70-71 -3.3409677675021143 1106 72-73 0.729250122420229 1106 74-75 1.5351098124756781 1106 76-77 1.6629885397707937 1106 78-79 1.4307956150265433 1106 80-81 1.0996189273830979 1106 82-83 0.9345382300542688 1106 84-85 0.8587850744746781 1106 86-87 1.0603046172403907 1106 88-89 0.9948809182385396 1106 90-91 1.0829973850668004 1106 92-93 0.9860789926836055 1106 94-95 1.2970969946255941 1106 96-97 1.3726617008942874 1106 98-99 1.137825751649018 1106 100-101 0.9856359614612096 1107 1 0.060859844960855725 1107 2 0.05476296231595512 1107 3 0.006401509245499426 1107 4 -0.09380343578067141 1107 5 0.3376687512880423 1107 6 0.2812329726620746 1107 7 0.037704780189770304 1107 8 0.008008243460075448 1107 9 0.19492324061747723 1107 10-11 0.10349483001683524 1107 12-13 0.1032863928457175 1107 14-15 0.04883410208257999 1107 16-17 0.05363126999566248 1107 18-19 0.18316856084832267 1107 20-21 0.21260591988332322 1107 22-23 0.22452433463976718 1107 24-25 0.12847347543784338 1107 26-27 0.46754485544716573 1107 28-29 0.31172770746962186 1107 30-31 0.23317947856065047 1107 32-33 0.23414149110995908 1107 34-35 0.319258311060004 1107 36-37 0.5012809925710613 1107 38-39 0.5600767847539174 1107 40-41 0.27701961670869224 1107 42-43 -0.14417468166001157 1107 44-45 0.19576434864682568 1107 46-47 0.6180172109619768 1107 48-49 0.42606465200832133 1107 50-51 0.2186356506016196 1107 52-53 -0.041161408639823094 1107 54-55 0.13703076654467594 1107 56-57 0.45440862199719945 1107 58-59 0.4729934108386473 1107 60-61 0.6585430492818567 1107 62-63 0.3853117135622064 1107 64-65 0.00946360384432765 1107 66-67 0.276104474404157 1107 68-69 -3.040099556653608 1107 70-71 -3.7093397824638643 1107 72-73 0.5172230915576108 1107 74-75 1.09719354842127 1107 76-77 0.7596839723892366 1107 78-79 0.5753744486665404 1107 80-81 0.5519576572632836 1107 82-83 0.4432007181918607 1107 84-85 0.1192317056744443 1107 86-87 0.11592064176364048 1107 88-89 0.3706733393789854 1107 90-91 0.3433823132383047 1107 92-93 0.44666001307892245 1107 94-95 0.8412529157098909 1107 96-97 0.5577214232553445 1107 98-99 0.675489280099665 1107 100-101 0.8487132395678643 1108 1 -0.038543733567969696 1108 2 0.19193990068573896 1108 3 0.1283365655741946 1108 4 0.09705143499467539 1108 5 -0.035426013456820726 1108 6 0.005553714875460969 1108 7 0.3637485177643214 1108 8 0.4241778922340984 1108 9 0.3658973956870568 1108 10-11 0.3970667319386365 1108 12-13 0.2007018998039669 1108 14-15 0.20754848246694024 1108 16-17 0.45323365568155083 1108 18-19 0.5181586204904747 1108 20-21 0.35309631087073257 1108 22-23 0.4770094241029881 1108 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0.4287611608417805 2311 20-21 -0.9259813526202123 2311 22-23 0.054237424640369625 2311 24-25 -0.040402535955472274 2311 26-27 0.4112271681580566 2311 28-29 0.5475004644541173 2311 30-31 0.547684837096547 2311 32-33 0.8824622423548973 2311 34-35 0.2638911951862539 2311 36-37 0.34978593113954304 2311 38-39 0.15191953856608365 2311 40-41 0.0759424341481747 2311 42-43 0.05385773711524422 2311 44-45 0.06742676483680299 2311 46-47 0.30136939767136184 2311 48-49 0.6054581942189969 2311 50-51 0.3268068957034096 2311 52-53 0.6025219201212835 2311 54-55 0.8087886914540476 2311 56-57 0.45655107675191786 2311 58-59 0.586680961852835 2311 60-61 0.9354712021138702 2311 62-63 0.8078771161495233 2311 64-65 0.9677702658597411 2311 66-67 1.1628027402322445 2311 68-69 3.157022086006048 2311 70-71 4.585104793690796 2311 72-73 3.637239224176998 2311 74-75 2.730048886579713 2311 76-77 2.2883656585358416 2311 78-79 1.634164807027446 2311 80-81 1.4428237045909036 2311 82-83 1.290617918567122 2311 84-85 1.2043741728475545 2311 86-87 1.015247847185286 2311 88-89 1.4006073074050143 2311 90-91 1.632751154139516 2311 92-93 1.5975491134567825 2311 94-95 1.7770371314828282 2311 96-97 1.9497610085713895 2311 98-99 2.0067385815651484 2311 100-101 2.291274792453841 2312 1 -0.2889430787901688 2312 2 -0.13427878224157297 2312 3 -0.08930225325412877 2312 4 0.06775643385721963 2312 5 -1.5852755480898182 2312 6 -0.41784742060023916 2312 7 -0.0732433370767751 2312 8 0.23028033231994982 2312 9 -2.9857872724236785 2312 10-11 -0.11923254948723638 2312 12-13 -1.592479620526781 2312 14-15 -4.104225772500765 2312 16-17 -1.0160498349171476 2312 18-19 -0.23272908808394988 2312 20-21 0.025054301731536555 2312 22-23 0.08425741624960636 2312 24-25 0.3807434377535017 2312 26-27 0.34410669090208756 2312 28-29 0.1781912397090224 2312 30-31 0.054603237005004246 2312 32-33 0.4592241419709566 2312 34-35 0.3409963529578732 2312 36-37 0.7413338347221412 2312 38-39 0.60006292512562 2312 40-41 0.11536547439836653 2312 42-43 -0.12828679999478254 2312 44-45 0.9311548662377973 2312 46-47 0.8069934282849012 2312 48-49 0.7679996712461268 2312 50-51 0.8624064214986262 2312 52-53 0.7105274631048317 2312 54-55 1.0009671729816745 2312 56-57 0.8955011835433453 2312 58-59 0.9080667395232567 2312 60-61 1.086562000252652 2312 62-63 1.118060123599495 2312 64-65 0.8393456300947832 2312 66-67 1.5293245816215517 2312 68-69 4.217972656831652 2312 70-71 5.152349225280716 2312 72-73 3.358942674557124 2312 74-75 2.6941982738003425 2312 76-77 2.8748412872067988 2312 78-79 1.8719568942912979 2312 80-81 1.7596017182703747 2312 82-83 1.6617856637424389 2312 84-85 1.8170272087752402 2312 86-87 1.6839314203755542 2312 88-89 1.777430866356422 2312 90-91 1.6588665842292762 2312 92-93 2.0195516433965217 2312 94-95 1.8657359923742831 2312 96-97 1.9204879212821062 2312 98-99 2.344390981829587 2312 100-101 2.17099379751372 2313 1 -0.3160798195976753 2313 2 -0.15459074374842885 2313 3 -0.21884382161127647 2313 4 -0.01057692156813772 2313 5 0.03119969116682597 2313 6 0.12264395025419361 2313 7 -3.299912500142444 2313 8 -3.588510924539623 2313 9 -1.10197918143988 2313 10-11 -0.3535756541239081 2313 12-13 -0.07984160059093881 2313 14-15 -0.6298033685348017 2313 16-17 -0.6098164039370602 2313 18-19 -0.12062972698535646 2313 20-21 -2.7342781987323903 2313 22-23 -0.7003362119680929 2313 24-25 -0.06136440668328902 2313 26-27 -1.6623610260195179 2313 28-29 -1.878771393837276 2313 30-31 -1.0299542707492932 2313 32-33 -1.3447966620904381 2313 34-35 -1.818714915301186 2313 36-37 -2.392330105788453 2313 38-39 -1.958791062043332 2313 40-41 -1.0709026189713704 2313 42-43 0.08843763732129872 2313 44-45 -0.15408445668576576 2313 46-47 -0.00900926252392864 2313 48-49 -2.1666943652322246 2313 50-51 -0.4327897225590611 2313 52-53 -0.19954915204323243 2313 54-55 -0.1965073770037442 2313 56-57 -0.3756798230577516 2313 58-59 -0.5013346880634977 2313 60-61 0.14588571913602877 2313 62-63 0.2768526397045825 2313 64-65 0.36608968513044005 2313 66-67 0.695573156685839 2313 68-69 1.189762243534016 2313 70-71 3.675700773489936 2313 72-73 2.9594536631311286 2313 74-75 1.6615631037359186 2313 76-77 1.5264345641612813 2313 78-79 1.08750327429183 2313 80-81 1.0613948176049597 2313 82-83 0.7876994451904409 2313 84-85 0.652635192391795 2313 86-87 1.1240468401682406 2313 88-89 0.5786001420914246 2313 90-91 0.9621036736489579 2313 92-93 1.2347914965287252 2313 94-95 1.5270204890737347 2313 96-97 1.5369490768718315 2313 98-99 1.1952093257229102 2313 100-101 1.3064231670956516 2314 1 -0.22591642240420384 2314 2 -0.30922164174479505 2314 3 -0.27141865464202297 2314 4 -0.06932912543120295 2314 5 -0.21900840594078375 2314 6 -2.9586237052287565 2314 7 -1.0053322008758627 2314 8 -0.37098226612692997 2314 9 -0.37966428955206055 2314 10-11 -0.48257210172604204 2314 12-13 -2.709027301861802 2314 14-15 -1.0973172799083741 2314 16-17 -2.113083692382588 2314 18-19 -0.5106659719304005 2314 20-21 -0.7793754636482078 2314 22-23 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2314 88-89 -0.8630228842631702 2314 90-91 -0.4392894299801249 2314 92-93 -0.9644868816902239 2314 94-95 -0.5270773853863631 2314 96-97 0.03424053735304611 2314 98-99 -0.3821831355906937 2314 100-101 -0.08310758211948155 2315 1 -0.3157156472823317 2315 2 -0.37507131378116654 2315 3 -0.43232781692513456 2315 4 -0.2629311581633331 2315 5 -0.5786085686735518 2315 6 -0.38773324293921974 2315 7 -1.0499837885909642 2315 8 -2.864883356008235 2315 9 -1.1399956791328805 2315 10-11 -1.473626260333397 2315 12-13 -0.7133829015106485 2315 14-15 -0.47580657737388066 2315 16-17 -0.5495750882443247 2315 18-19 -0.3016542880031139 2315 20-21 -1.054566032285031 2315 22-23 -0.28435437248563034 2315 24-25 -5.772761282359667 2315 26-27 -1.1694555149929968 2315 28-29 -1.0780535277496242 2315 30-31 -1.603286894061128 2315 32-33 -0.43852207616466643 2315 34-35 -2.2509384964892796 2315 36-37 -3.6529778515274636 2315 38-39 -1.0155212069220738 2315 40-41 -0.21227190687707065 2315 42-43 -1.8867617126654714 2315 44-45 -5.717974366707722 2315 46-47 -4.264033809799333 2315 48-49 -2.635437386694342 2315 50-51 -1.4284479983749243 2315 52-53 -0.18657551330903743 2315 54-55 -0.9389722738845734 2315 56-57 -0.8841339459349875 2315 58-59 -0.36655056322797464 2315 60-61 -1.7382525151779333 2315 62-63 -0.3091985299413089 2315 64-65 0.10137506223774295 2315 66-67 0.1672742193131711 2315 68-69 2.9101079667424443 2315 70-71 3.5265850938104073 2315 72-73 -2.355912690782521 2315 74-75 -1.6821468501928472 2315 76-77 -0.8015927899894244 2315 78-79 -0.4969950096320268 2315 80-81 -0.9440325523494444 2315 82-83 -1.6768238154573076 2315 84-85 -1.005613997668359 2315 86-87 -0.38548004502471755 2315 88-89 -0.41691383903345525 2315 90-91 -0.2421454614417904 2315 92-93 -1.6453004741588373 2315 94-95 -0.3734831681095656 2315 96-97 -0.5362091903550628 2315 98-99 -2.5600222157223094 2315 100-101 -3.6150358583654914 2316 1 -0.48568663374268084 2316 2 -0.35108678766898294 2316 3 -0.926089905899989 2316 4 -0.26904817943992754 2316 5 -0.23588613153622617 2316 6 -0.6641839199218111 2316 7 -0.7242100942002523 2316 8 -1.00460772738154 2316 9 -4.8148747893843336 2316 10-11 -1.2922311926351355 2316 12-13 -0.6147489556692491 2316 14-15 -0.3079995174125685 2316 16-17 -0.52235071687101 2316 18-19 -0.28155032281936343 2316 20-21 -3.6516163224204305 2316 22-23 -1.368396925677125 2316 24-25 -0.43295228816237596 2316 26-27 -0.8534255343353578 2316 28-29 -0.17787219312679525 2316 30-31 0.08828709046498062 2316 32-33 -1.4314983817739488 2316 34-35 -0.4672465235685834 2316 36-37 -0.4533405207731178 2316 38-39 -5.106865500925942 2316 40-41 -4.8940900886952505 2316 42-43 -3.7512684824913904 2316 44-45 -2.222084618158398 2316 46-47 -2.5377402894898573 2316 48-49 -2.769008469866492 2316 50-51 -1.3874663736167072 2316 52-53 -0.9790078150498509 2316 54-55 -0.4058001268826388 2316 56-57 -3.383577853091662 2316 58-59 -1.0592246444658855 2316 60-61 -0.6467147975763865 2316 62-63 -0.2810070405796061 2316 64-65 0.05170146455882829 2316 66-67 0.17304066031897491 2316 68-69 2.7988410421776457 2316 70-71 3.9511619796904824 2316 72-73 2.653057328559843 2316 74-75 1.1597172697297857 2316 76-77 -1.6798568132002565 2316 78-79 0.09183477760201697 2316 80-81 0.24297179968150218 2316 82-83 0.4948758944072935 2316 84-85 1.0415692711904398 2316 86-87 0.8446335913389156 2316 88-89 0.6638284240226362 2316 90-91 0.3479778461017311 2316 92-93 0.4384882105606991 2316 94-95 0.992389655874959 2316 96-97 1.4131863609021913 2316 98-99 1.2196199506219862 2316 100-101 1.2353509888298682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 37.0 4 88.0 5 263.0 6 618.0 7 1375.0 8 2766.0 9 4317.0 10 6637.0 11 9232.0 12 11427.0 13 14339.0 14 17643.0 15 21565.0 16 24546.0 17 26791.0 18 24664.0 19 22691.0 20 22285.0 21 22723.0 22 23584.0 23 26159.0 24 30282.0 25 35975.0 26 43552.0 27 53076.0 28 63226.0 29 73653.0 30 83500.0 31 93285.0 32 103304.0 33 112566.0 34 119428.0 35 117465.0 36 96156.0 37 59118.0 38 15151.0 39 483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.63563688711657 22.387851527664758 15.794661737052898 21.181849848165776 2 17.237120524487047 23.369288716475207 37.207823367192965 22.18576739184478 3 20.26397913832134 26.251451437275147 31.105231099161617 22.379338325241893 4 13.856906616263103 18.85346836629257 36.07205067929841 31.21757433814592 5 12.62346655799442 39.35293769120607 33.86173768658172 14.161858064217784 6 30.767685613499633 37.85970586237642 16.629671858945734 14.742936665178203 7 26.75020845707085 34.73653482931087 20.66456258697754 17.84869412664074 8 26.461114266267238 35.71350122617556 19.311289630326495 18.514094877230708 9 26.214867577374783 16.4100151881027 20.697872365023144 36.67724486949937 10-11 24.9516566800496 28.024998337972555 26.518840164988745 20.5045048169891 12-13 25.596649657725774 25.56977061774103 28.04163071956346 20.79194900496973 14-15 22.324921349905853 26.552878730731265 27.319112009005924 23.803087910356957 16-17 20.721177364009268 29.800075579509137 27.97937532018327 21.499371736298322 18-19 20.449972000939322 27.274553369822428 31.985006954605392 20.29046767463285 20-21 21.535494061321785 26.384667964375158 32.157194695291395 19.92264327901166 22-23 20.905600964178568 26.604399209234842 32.12906148661537 20.360938339971213 24-25 20.661498308445346 26.747265404275062 32.4665410344713 20.124695252808298 26-27 20.75705719495536 26.990236822890267 32.128920327662385 20.12378565449199 28-29 20.406564777994184 27.137423981808745 32.31693945243419 20.139071787762884 30-31 20.547993210869834 26.946184799313862 32.35588452698275 20.14993746283356 32-33 20.617419229672887 26.98341474764094 32.04439690007843 20.354769122607745 34-35 20.776808607570608 27.084648264435458 32.09398931821327 20.044553809780666 36-37 20.868914090594533 26.877322586733403 32.01067766202155 20.243085660650507 38-39 20.669966841559063 27.24817483936055 31.94607866180198 20.135779657278405 40-41 20.7925710474035 27.10822922447563 31.904473532458283 20.19472619566259 42-43 20.666863592763583 27.221031707150406 31.62995875037014 20.482145949715875 44-45 20.937244992589985 27.382096866389404 31.28400626064094 20.396651880379675 46-47 21.028234713939764 27.70240711294681 30.620170173318783 20.64918799979464 48-49 21.13329513142601 27.828185415427665 30.409200272595317 20.62931918055101 50-51 21.05612508297072 28.081268645816614 30.175348404720694 20.687257866491976 52-53 21.243188435658166 28.22477303822428 29.86511521145445 20.666923314663098 54-55 20.95519919441032 28.096096786424397 30.05372164143958 20.894982377725704 56-57 21.15081894701237 28.38351380378518 29.660213962645436 20.80545328655701 58-59 21.350544454313464 28.055387123747632 29.77948642712652 20.814581994812382 60-61 21.364506006117225 28.105990941593696 30.02191433356398 20.507588718725103 62-63 21.131294957530827 28.060183027504703 30.044799468186955 20.76372254677751 64-65 21.05321346736239 28.011553033177034 29.96334939687646 20.971884102584113 66-67 21.14062080988073 28.25607439498523 29.6547079343239 20.94859686081014 68-69 22.39589060348387 26.882969729319573 30.547798168277573 20.17334149891898 70-71 22.351935481400464 26.736459215814847 30.812488990824683 20.099116311960003 72-73 21.811080446931427 27.593446166232088 29.919418887603495 20.676054499232993 74-75 21.107538484061443 28.339017605882 29.315013528387922 21.23843038166863 76-77 20.676085771200047 28.395653077465038 29.737918919525598 21.190342231809318 78-79 20.994005212513066 28.369967744278284 29.36994209001323 21.266084953195417 80-81 20.7648255537736 28.4884096922077 29.354128603443876 21.39263615057483 82-83 21.087137948849495 28.53915047815861 29.022619955128597 21.351091617863297 84-85 20.823157337988523 28.46938819748319 29.031175396105134 21.676279068423153 86-87 20.829094277563737 28.40213128163034 29.07137221125527 21.69740222955065 88-89 20.8947885050935 28.408393393062493 28.919820848653092 21.776997253190913 90-91 20.764769087252812 28.56868274995466 28.995007663009666 21.671540499782864 92-93 20.861101817816582 28.415081005814674 29.011650298826584 21.712166877542156 94-95 20.800628284064235 28.095607941487433 29.148424878544766 21.95533889590357 96-97 20.698180998081934 28.153960085252272 29.06962329322872 22.078235623437077 98-99 20.620614399583754 28.11340593587177 28.876527507786587 22.389452156757887 100-101 20.53191520112849 28.090493368945623 29.042045336293317 22.33554609363257 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4962.0 1 3476.0 2 2028.5 3 2137.5 4 1956.0 5 1759.0 6 2567.5 7 4568.5 8 6280.0 9 6768.0 10 5863.0 11 4432.0 12 3788.0 13 3558.5 14 3461.5 15 3451.0 16 3522.5 17 3718.5 18 3878.5 19 4097.0 20 4532.5 21 5116.5 22 5823.0 23 6731.0 24 7668.5 25 8992.0 26 11022.0 27 13226.5 28 15246.5 29 17074.5 30 19165.0 31 21924.0 32 24728.0 33 27344.0 34 30085.5 35 32741.5 36 35791.5 37 38530.5 38 40062.0 39 40902.0 40 41729.5 41 43042.0 42 44313.0 43 45289.0 44 46322.0 45 47277.0 46 47549.0 47 47540.5 48 48773.0 49 48702.5 50 46452.5 51 44170.5 52 41811.0 53 40347.0 54 38423.0 55 35109.5 56 31718.0 57 28364.0 58 25412.0 59 22528.0 60 19280.5 61 15852.5 62 12796.0 63 9940.5 64 7495.0 65 5782.0 66 4321.5 67 3281.5 68 2388.5 69 1655.0 70 1256.0 71 1015.5 72 839.0 73 707.5 74 602.0 75 491.0 76 331.5 77 204.5 78 136.5 79 83.5 80 51.0 81 33.5 82 24.0 83 18.5 84 9.5 85 2.0 86 1.5 87 2.5 88 2.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016763272248547303 2 0.016329739345567633 3 3.612774191497264E-4 4 0.0727612722167549 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009104190962573104 12-13 0.0 14-15 0.0 16-17 3.612774191497264E-4 18-19 2.1676645148983582E-4 20-21 0.0 22-23 1.4451096765989057E-4 24-25 0.0031792412885175924 26-27 0.003865668384902072 28-29 0.001192215483194097 30-31 7.225548382994528E-5 32-33 5.419161287245896E-4 34-35 0.0024205587083031667 36-37 0.0788307328584703 38-39 0.08836845672402308 40-41 0.0730141664101597 42-43 0.07633791866633718 44-45 0.0760488967310174 46-47 0.07478442576399336 48-49 0.07048522447611162 50-51 0.06936526447674747 52-53 0.05495029545267338 54-55 0.04649640384456979 56-57 0.026879039984739644 58-59 0.02160438966515364 60-61 0.013042114831305122 62-63 0.002384430966388194 64-65 0.0071532928991645825 66-67 0.17193192377335478 68-69 0.7756626189144625 70-71 0.9366117091456657 72-73 0.37883550172040303 74-75 0.056034127710122554 76-77 0.2135149547174883 78-79 0.013909180637264464 80-81 0.0029986025789427288 82-83 0.0016618761280887413 84-85 1.4451096765989057E-4 86-87 0.0015896206442587962 88-89 6.141716125545348E-4 90-91 0.00411856257830688 92-93 0.004696606448946442 94-95 0.0841053831780563 96-97 0.04006566578370466 98-99 0.01358403096002971 100-101 0.013439519992369822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1383978.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.77044480300965 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53266594842353 49.7453859680631 2 11.599223660733324 14.329784097861825 3 3.635437015696147 6.736876845386308 4 1.5260985039511918 3.770711336090908 5 0.7696067705873052 2.376947627129282 6 0.43649011404556487 1.617731309802657 7 0.29311667182939105 1.2674163038655506 8 0.19345349511904122 0.9559766753760027 9 0.14300257876065486 0.7949999608220765 >10 0.7762122842762564 8.891998298904879 >50 0.06398399230466663 2.7275957244147073 >100 0.02905543079048106 3.109304861735338 >500 4.724381378310998E-4 0.1925329550590459 >1k 7.086572067466497E-4 0.8899998176450554 >5k 2.362190689155499E-4 1.0059281486753031 >10k+ 2.362190689155499E-4 1.586810069167961 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11476 0.8292039324324519 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10274 0.7423528408688578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8174 0.5906163248259727 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5614 0.40564228622131276 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3188 0.23035048244986553 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.17767623473783542 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 0.16813851087228265 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.1083832257449179 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.10744390445512862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.6127741914972635E-5 0.0 18-19 0.0 0.0 0.0 7.225548382994527E-5 0.0 20-21 0.0 0.0 0.0 2.528941934048085E-4 0.0 22-23 0.0 0.0 0.0 4.3353290297967165E-4 0.0 24-25 0.0 0.0 0.0 7.225548382994528E-4 0.0 26-27 0.0 0.0 0.0 0.001083832257449179 0.0 28-29 0.0 0.0 0.0 0.0020592812891534404 0.0 30-31 0.0 0.0 0.0 0.005130139351926114 0.0 32-33 0.0 0.0 0.0 0.011669260638536162 0.0 34-35 0.0 0.0 0.0 0.022615966438772873 0.0 36-37 0.0 0.0 0.0 0.04067983739625919 0.0 38-39 0.0 0.0 0.0 0.06799241028397851 0.0 40-41 0.0 0.0 0.0 0.10029061155596404 0.0 42-43 0.0 0.0 0.0 0.13099919218369077 0.0 44-45 0.0 0.0 0.0 0.16839140506568745 0.0 46-47 0.0 0.0 0.0 0.21716385665090052 0.0 48-49 0.0 0.0 0.0 0.27095806436229475 0.0 50-51 0.0 0.0 0.0 0.3286179404585911 0.0 52-53 0.0 0.0 0.0 0.38851773655361577 0.0 54-55 0.0 0.0 0.0 0.4558598474831247 0.0 56-57 0.0 0.0 0.0 0.5307526564728631 0.0 58-59 0.0 0.0 0.0 0.604633888688982 0.0 60-61 0.0 0.0 0.0 0.6805744021942546 3.6127741914972635E-5 62-63 0.0 0.0 0.0 0.7587187079563403 7.225548382994527E-5 64-65 0.0 0.0 0.0 0.8412344704901378 7.225548382994527E-5 66-67 7.225548382994527E-5 0.0 0.0 0.9295306717303309 7.225548382994527E-5 68-69 7.225548382994527E-5 0.0 0.0 1.0346985284448165 7.225548382994527E-5 70-71 7.225548382994527E-5 0.0 0.0 1.1747296561072502 7.225548382994527E-5 72-73 7.225548382994527E-5 0.0 0.0 1.32700808827886 7.225548382994527E-5 74-75 7.225548382994527E-5 0.0 0.0 1.4881739449615528 7.225548382994527E-5 76-77 7.225548382994527E-5 0.0 0.0 1.6774833125960096 7.225548382994527E-5 78-79 7.225548382994527E-5 0.0 0.0 1.9003914802113906 1.0838322574491791E-4 80-81 7.225548382994527E-5 0.0 0.0 2.156067509743652 1.4451096765989054E-4 82-83 7.225548382994527E-5 0.0 0.0 2.4162956347571996 1.4451096765989054E-4 84-85 7.225548382994527E-5 0.0 0.0 2.66091657526348 1.4451096765989054E-4 86-87 7.225548382994527E-5 0.0 0.0 2.9303934022072604 1.4451096765989054E-4 88-89 7.225548382994527E-5 0.0 0.0 3.228230506554295 1.4451096765989054E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5500 0.0 46.50719 1 GTATCAA 8985 0.0 36.708004 1 TATCAAC 12180 0.0 27.108091 2 ATCAACG 12200 0.0 26.904219 3 TCAACGC 12335 0.0 26.461697 4 CAACGCA 12545 0.0 25.998047 5 AACGCAG 12815 0.0 25.450294 6 GTACATG 7610 0.0 25.06891 1 TACATGG 7810 0.0 24.175142 2 ACATGGG 7890 0.0 22.964687 3 ACGCAGA 14755 0.0 21.879171 7 CGCAGAG 14840 0.0 21.65802 8 TATAGCG 110 0.0029113274 21.547668 5 GAACCGT 110 0.0029113274 21.547668 6 AGTACTT 9790 0.0 20.889448 12-13 GAGTACT 9600 0.0 20.784504 12-13 GCAGAGT 15165 0.0 20.66246 9 GTATAGC 555 0.0 20.52165 1 AGAGTAC 13615 0.0 20.261951 10-11 TAGCCCT 995 0.0 20.026018 4 >>END_MODULE