FastQCFastQC Report
Fri 27 May 2016
SRR939357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000260
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168440.8420905282313299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114330.5715756951596292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87500.437443132392789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35440.17717696699429075No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.1214842070530831No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.1205343305370302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96750.038.945071
GTATCAA155400.037.1640051
ATCAACG202250.028.4157813
TATCAAC203050.028.3972362
TCAACGC205800.027.9947364
CAACGCA209400.027.5587445
AACGCAG214250.026.9830366
TACATGG164200.024.5465952
GTACATG166650.024.517761
ACGCAGA237650.024.2065377
CGCAGAG240150.023.9156788
GAGTACT120000.023.45587212-13
AGTACTT122750.022.98828912-13
ACATGGG169100.022.741693
GCAGAGT248800.022.6450049
GTACTTT126100.022.09989414-15
CATGGGG51650.021.3914
TACTTTT136600.020.34910214-15
AGTATCG3102.6557245E-1019.885075
CATGGGA112200.019.8632584
Produced by FastQC (version 0.11.5)