##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939357_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000260 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95394198754162 33.0 31.0 34.0 30.0 34.0 2 31.99163608730865 33.0 31.0 34.0 30.0 34.0 3 32.025537680101586 34.0 31.0 34.0 30.0 34.0 4 35.540308259926206 37.0 35.0 37.0 33.0 37.0 5 35.58374911261536 37.0 35.0 37.0 33.0 37.0 6 35.69518412606361 37.0 35.0 37.0 34.0 37.0 7 35.63340115784948 37.0 35.0 37.0 33.0 37.0 8 35.62009888714467 37.0 35.0 37.0 33.0 37.0 9 37.23587283653125 39.0 37.0 39.0 34.0 39.0 10-11 37.13244728185336 39.0 37.0 39.0 33.5 39.0 12-13 37.021168998030255 39.0 37.0 39.0 33.0 39.0 14-15 38.20369326987492 40.0 38.0 41.0 33.0 41.0 16-17 38.14556382670253 40.0 38.0 41.0 33.0 41.0 18-19 38.125725155729754 40.0 38.0 41.0 33.0 41.0 20-21 38.12229685140932 40.0 38.0 41.0 33.0 41.0 22-23 38.11042314499115 40.0 38.0 41.0 33.0 41.0 24-25 38.01260061192045 40.0 38.0 41.0 33.0 41.0 26-27 37.90898333216682 40.0 38.0 41.0 33.0 41.0 28-29 37.74523386959696 40.0 37.5 41.0 33.0 41.0 30-31 37.49164783578135 40.0 37.0 41.0 32.0 41.0 32-33 37.20346329977103 40.0 36.0 41.0 31.5 41.0 34-35 36.881181446411965 40.0 35.5 41.0 31.0 41.0 36-37 36.612571865657465 39.5 35.0 41.0 30.0 41.0 38-39 35.98869321988141 38.5 35.0 40.5 29.0 41.0 40-41 35.90987971563697 39.0 35.0 40.0 30.0 41.0 42-43 35.97666728325318 39.0 35.0 41.0 29.5 41.0 44-45 35.75544329237199 39.0 35.0 41.0 27.5 41.0 46-47 35.33042079529661 39.0 35.0 41.0 25.5 41.0 48-49 34.96722326097607 39.0 35.0 41.0 24.0 41.0 50-51 33.79560057192565 38.0 33.5 40.0 21.5 40.5 52-53 33.86985091938048 38.0 33.5 39.5 22.0 40.5 54-55 34.31736599242099 38.0 34.0 40.0 22.0 41.0 56-57 34.215619969403974 38.0 34.0 40.0 21.5 41.0 58-59 33.991047663803705 38.0 34.0 40.0 20.0 41.0 60-61 33.67594437723096 37.5 34.0 40.0 18.5 41.0 62-63 33.28358788357514 37.0 33.5 40.0 16.5 41.0 64-65 32.88605981222441 36.5 33.0 40.0 13.0 41.0 66-67 32.51526826512553 36.0 33.0 39.0 9.0 41.0 68-69 32.10902832631758 35.5 32.0 39.0 7.0 40.5 70-71 31.688337016187894 35.0 32.0 38.5 4.0 40.0 72-73 31.254926359573254 35.0 31.0 37.5 2.0 40.0 74-75 30.800186475758153 35.0 31.0 37.0 2.0 39.0 76-77 30.341492605961225 35.0 30.5 36.5 2.0 39.0 78-79 29.952630408046954 35.0 30.0 36.0 2.0 38.5 80-81 29.54958955335806 34.0 30.0 35.5 2.0 37.0 82-83 29.11924149860518 34.0 29.0 35.0 2.0 37.0 84-85 28.69880165578475 34.0 29.0 35.0 2.0 36.5 86-87 28.255914481117454 34.0 29.0 35.0 2.0 36.0 88-89 27.820463589733336 34.0 27.5 35.0 2.0 36.0 90-91 27.395509083819103 34.0 26.5 35.0 2.0 35.0 92-93 26.93832376791017 33.5 25.5 35.0 2.0 35.0 94-95 26.442063031805866 33.0 25.0 35.0 2.0 35.0 96-97 25.825644666193398 33.0 23.5 35.0 2.0 35.0 98-99 25.02356693629828 33.0 10.5 35.0 2.0 35.0 100-101 22.356327427434433 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17327983125593605 1101 2 0.42084601716403824 1101 3 0.470599193238435 1101 4 0.31892755134781936 1101 5 0.27461422319278483 1101 6 0.370024080653657 1101 7 0.21765723975529738 1101 8 0.14557598950619877 1101 9 0.251833698646756 1101 10-11 0.415115060590054 1101 12-13 0.44139792029256597 1101 14-15 0.4861225864015424 1101 16-17 0.6740249827646281 1101 18-19 0.7134747566694628 1101 20-21 0.6134320313778829 1101 22-23 0.7442462204587983 1101 24-25 0.5932215107216834 1101 26-27 0.6172484306354633 1101 28-29 0.7870307561953211 1101 30-31 0.7336935120159964 1101 32-33 0.6939384889380094 1101 34-35 0.6224771057076381 1101 36-37 0.4362054595094449 1101 38-39 0.7456118924542423 1101 40-41 0.7290915288598327 1101 42-43 0.7317556325594694 1101 44-45 0.6657954315035255 1101 46-47 0.7649490182217349 1101 48-49 0.6980595076418084 1101 50-51 0.6836921111202017 1101 52-53 0.6777311514567756 1101 54-55 0.7615699553301525 1101 56-57 0.7412614783651037 1101 58-59 0.731663338830451 1101 60-61 0.6793234338627059 1101 62-63 0.7525456595111848 1101 64-65 0.6402816939281593 1101 66-67 0.6668718256188981 1101 68-69 0.7493365170856947 1101 70-71 0.6413974209609741 1101 72-73 0.7094001263181617 1101 74-75 0.5569703488189042 1101 76-77 0.5582375797756534 1101 78-79 0.5847567146217365 1101 80-81 0.5337639687145384 1101 82-83 0.47480240976912214 1101 84-85 0.4399129499566534 1101 86-87 0.33808436943709097 1101 88-89 0.33370980284705 1101 90-91 0.582902725487564 1101 92-93 0.5525720882894909 1101 94-95 0.6674604134376487 1101 96-97 0.6977888518766555 1101 98-99 0.7172771256750607 1101 100-101 0.1967871007623394 1102 1 0.3538293331239295 1102 2 0.3301963700075419 1102 3 0.5153791849362364 1102 4 0.385342557574468 1102 5 0.37172661422641085 1102 6 0.3758537817744596 1102 7 0.3244442883231713 1102 8 0.3635554415609974 1102 9 0.3665156206060729 1102 10-11 0.4008054404157022 1102 12-13 0.42918330842041286 1102 14-15 0.552939730444713 1102 16-17 0.5334103605147291 1102 18-19 0.5293254727342216 1102 20-21 0.5417308586921195 1102 22-23 0.6179931071337705 1102 24-25 0.5919800723655158 1102 26-27 0.6268660105441413 1102 28-29 0.5063242908570018 1102 30-31 0.5358754339753133 1102 32-33 0.541612575696 1102 34-35 0.5378595257989573 1102 36-37 0.48375760770372267 1102 38-39 0.9366935134588132 1102 40-41 0.7146028924961954 1102 42-43 0.8370897961128207 1102 44-45 1.0338682833092534 1102 46-47 0.7299202199652015 1102 48-49 0.7879710373221727 1102 50-51 0.6426481094597634 1102 52-53 0.7832884366373492 1102 54-55 0.7419573048527823 1102 56-57 0.7844069744215574 1102 58-59 0.6676222429400411 1102 60-61 0.6288395712641162 1102 62-63 0.7524237209474691 1102 64-65 0.784208115679931 1102 66-67 0.8084100676280315 1102 68-69 0.7227487213198103 1102 70-71 0.8319523711477679 1102 72-73 0.8400222724368795 1102 74-75 0.654848098922681 1102 76-77 0.7317055644165826 1102 78-79 0.5797001558836747 1102 80-81 0.550468254725331 1102 82-83 0.48498298262093087 1102 84-85 0.5507395377524169 1102 86-87 0.5559211274279612 1102 88-89 0.4026258468487107 1102 90-91 0.5921155731421948 1102 92-93 0.5549278375630458 1102 94-95 0.35939300995072543 1102 96-97 0.2511655123166747 1102 98-99 0.31315067279004793 1102 100-101 0.5283108139213297 1103 1 0.33271075275867545 1103 2 0.4340834651543517 1103 3 0.3683960349009183 1103 4 0.4074764239855284 1103 5 0.3255222705678449 1103 6 0.42572702867184375 1103 7 0.4212685240208742 1103 8 0.42597220071748865 1103 9 0.5320785052796708 1103 10-11 0.5484220406578544 1103 12-13 0.5296411330597053 1103 14-15 0.6881833990957062 1103 16-17 0.7693990977763505 1103 18-19 0.7016034340902877 1103 20-21 0.6785192125413744 1103 22-23 0.6826289915046715 1103 24-25 0.6779019647073667 1103 26-27 0.6674251115430323 1103 28-29 0.5515168168382303 1103 30-31 0.6163245561627804 1103 32-33 0.6506754225328493 1103 34-35 0.4928054192506792 1103 36-37 0.3324033674656022 1103 38-39 0.8889375478650194 1103 40-41 0.774388421201671 1103 42-43 0.6625551881015141 1103 44-45 0.6346779896960939 1103 46-47 0.5914241675991434 1103 48-49 0.49746396185047104 1103 50-51 0.4164978723494883 1103 52-53 0.5792323373537229 1103 54-55 0.6967174829377356 1103 56-57 0.6411507439705488 1103 58-59 0.6371422207424828 1103 60-61 0.7648131738269228 1103 62-63 0.6707108514085647 1103 64-65 0.7522076868634571 1103 66-67 0.7832262440986213 1103 68-69 0.6391307715670109 1103 70-71 0.6602539426340037 1103 72-73 0.738170163668844 1103 74-75 0.5482468477875813 1103 76-77 0.6392019699256153 1103 78-79 0.7334125713816029 1103 80-81 0.7411014526871895 1103 82-83 0.7210489699077804 1103 84-85 0.5406163160795323 1103 86-87 0.4055534803691323 1103 88-89 0.4304503600159002 1103 90-91 0.5133779331462307 1103 92-93 0.5574490261413949 1103 94-95 0.5559224343276803 1103 96-97 0.4228267346958461 1103 98-99 0.20304794602181175 1103 100-101 -0.03641997762341731 1104 1 0.2337648368094314 1104 2 0.30603175084292644 1104 3 0.3153054748625266 1104 4 0.26428297526489075 1104 5 0.19474565599544746 1104 6 0.22890967858035083 1104 7 0.35364576252464275 1104 8 0.2861905766961286 1104 9 0.2599646378050906 1104 10-11 0.3959851087203674 1104 12-13 0.31654817328527685 1104 14-15 0.3708267083316912 1104 16-17 0.24644322292258636 1104 18-19 0.36130796659171693 1104 20-21 0.40241728375355024 1104 22-23 0.5003180457087169 1104 24-25 0.43942939668984593 1104 26-27 0.3661565510846785 1104 28-29 0.39018946284717515 1104 30-31 0.3107433700932489 1104 32-33 0.1993321702905959 1104 34-35 0.2637041203935482 1104 36-37 0.223015900087006 1104 38-39 0.7632306756209672 1104 40-41 0.5169969342652436 1104 42-43 0.29290674942976835 1104 44-45 0.38567418011515286 1104 46-47 0.3697620504320369 1104 48-49 0.19832576705189808 1104 50-51 0.17482255663421853 1104 52-53 0.18756362091253465 1104 54-55 0.14590847192894785 1104 56-57 0.18056790808249445 1104 58-59 0.09971683753463623 1104 60-61 0.2452385270380688 1104 62-63 0.04240836468211029 1104 64-65 -0.04104065581884697 1104 66-67 0.11352831087127413 1104 68-69 0.011034962106052149 1104 70-71 0.22179727286767204 1104 72-73 0.17113713730174496 1104 74-75 0.17554834462292845 1104 76-77 0.0556536602396811 1104 78-79 0.015827305760822696 1104 80-81 -0.11946878458670795 1104 82-83 -0.08614723850929451 1104 84-85 -0.09641341065053055 1104 86-87 -0.18465319689636672 1104 88-89 -0.19381457084170606 1104 90-91 -0.20574376346714374 1104 92-93 -0.23004144990624198 1104 94-95 -0.22912511044239992 1104 96-97 -0.09319720267104259 1104 98-99 -0.20635331983894645 1104 100-101 -0.3363743826388692 1105 1 0.2923281369516779 1105 2 0.3852920637874462 1105 3 0.37772368254388766 1105 4 0.28864997384241065 1105 5 0.3424981453268856 1105 6 0.4144004324921582 1105 7 0.27001845100259203 1105 8 0.38842386261362805 1105 9 0.5160670560127727 1105 10-11 0.5237091485496705 1105 12-13 0.48036751894672847 1105 14-15 0.5904995390571557 1105 16-17 0.5728870351558797 1105 18-19 0.5408954488107796 1105 20-21 0.6225950931418822 1105 22-23 0.6154033942151145 1105 24-25 0.6003682302602584 1105 26-27 0.5606160105441447 1105 28-29 0.6117399607134573 1105 30-31 0.6384531851714854 1105 32-33 0.5165796809591612 1105 34-35 0.545688473167381 1105 36-37 0.5670201675123252 1105 38-39 0.992121145037757 1105 40-41 0.8271178446493082 1105 42-43 0.4964558248209485 1105 44-45 0.634059670869064 1105 46-47 0.6679555070465355 1105 48-49 0.5825434057432162 1105 50-51 0.3803275352970914 1105 52-53 0.4580970490775442 1105 54-55 0.6658657976757425 1105 56-57 0.595375873943091 1105 58-59 0.6556485587295171 1105 60-61 0.7193210305942586 1105 62-63 0.5100612807560836 1105 64-65 0.5069892161583951 1105 66-67 0.5623903307859308 1105 68-69 0.6801530275399088 1105 70-71 0.7365127778463325 1105 72-73 0.713114377700041 1105 74-75 0.6596806348078523 1105 76-77 0.7769836744644323 1105 78-79 0.8009980027736248 1105 80-81 0.7114700489837027 1105 82-83 0.5490021213769083 1105 84-85 0.6468938439725136 1105 86-87 0.5657237590069037 1105 88-89 0.5302394832123483 1105 90-91 0.7572292095058302 1105 92-93 0.7683369284721344 1105 94-95 0.8334211199985724 1105 96-97 0.9802594118382046 1105 98-99 1.0070890699192319 1105 100-101 0.5725303115289861 1106 1 0.05634112502330524 1106 2 0.10802173256444547 1106 3 0.1495721731560664 1106 4 0.13234559648186917 1106 5 0.11623751440006203 1106 6 0.009618213651307883 1106 7 0.05599377910084513 1106 8 0.04594444517483254 1106 9 0.11905322561851506 1106 10-11 0.21333961345742836 1106 12-13 0.16238542509568532 1106 14-15 0.20193067238867002 1106 16-17 0.23419399382772355 1106 18-19 0.20560131518036684 1106 20-21 0.24366552218299375 1106 22-23 0.2230140509550509 1106 24-25 0.14773800989778607 1106 26-27 0.24463511224781342 1106 28-29 0.2485353306561251 1106 30-31 0.12375764404711731 1106 32-33 0.28139219624323886 1106 34-35 0.11505686704831675 1106 36-37 0.12144548610048389 1106 38-39 0.6256890606968071 1106 40-41 0.45279441992849456 1106 42-43 0.43860279653520706 1106 44-45 0.477683696084398 1106 46-47 0.4517793801003691 1106 48-49 0.39780099343987985 1106 50-51 0.3184412760247497 1106 52-53 0.32350761343370493 1106 54-55 0.3689280480357766 1106 56-57 0.44597066241142613 1106 58-59 0.4574454231155727 1106 60-61 0.492433548595514 1106 62-63 0.5060173169487783 1106 64-65 0.49627310605868047 1106 66-67 0.4612608033017551 1106 68-69 0.44593652345056967 1106 70-71 0.6593499106887641 1106 72-73 0.5344240535416809 1106 74-75 0.6734839932297199 1106 76-77 0.703834882246408 1106 78-79 0.7743272150661014 1106 80-81 0.828060272606777 1106 82-83 0.6781964805324101 1106 84-85 0.7251889557840521 1106 86-87 0.887590590515206 1106 88-89 0.8419741244100756 1106 90-91 0.699010314307067 1106 92-93 0.6358537069956256 1106 94-95 0.9314158486891664 1106 96-97 1.1276378864190804 1106 98-99 0.9243903768779056 1106 100-101 0.5326235104529857 1107 1 0.13162004141162598 1107 2 0.09539352225288411 1107 3 0.12691624225642073 1107 4 0.07279920233533232 1107 5 0.04395872448562699 1107 6 0.05165471277116751 1107 7 0.08028583633338826 1107 8 -0.02193652631049048 1107 9 0.11639468230742267 1107 10-11 0.16232994753508478 1107 12-13 0.1726070031995306 1107 14-15 0.2677215851362078 1107 16-17 0.278717726341668 1107 18-19 0.34345357605660354 1107 20-21 0.21166400034965704 1107 22-23 0.18417373875114862 1107 24-25 0.26639021292777443 1107 26-27 0.2105256490983649 1107 28-29 0.17217063076207495 1107 30-31 0.06863602178108863 1107 32-33 0.1470970134470022 1107 34-35 -0.0014226027151025278 1107 36-37 0.09572023092386672 1107 38-39 0.5062090757078082 1107 40-41 0.19269594653051314 1107 42-43 0.0014061524472452902 1107 44-45 0.2705878890047657 1107 46-47 0.2895370965625048 1107 48-49 0.31167836663943405 1107 50-51 0.2207788140964979 1107 52-53 0.36870738515870727 1107 54-55 0.36772058524708484 1107 56-57 0.17252763889764822 1107 58-59 0.19031300599225176 1107 60-61 0.2824620155868658 1107 62-63 0.2139219867379012 1107 64-65 0.1309017082319528 1107 66-67 0.15192161381277458 1107 68-69 0.21151743252828226 1107 70-71 0.37347642189621766 1107 72-73 0.21961564593085825 1107 74-75 0.16914163671019722 1107 76-77 0.2407725140332353 1107 78-79 0.3069021456609633 1107 80-81 0.2727129151853518 1107 82-83 0.27928747331096204 1107 84-85 0.293649815225951 1107 86-87 0.5997715290343848 1107 88-89 0.595641089638054 1107 90-91 0.6521488882206867 1107 92-93 0.6970919485524583 1107 94-95 0.6526718069569313 1107 96-97 0.7175115783991828 1107 98-99 0.9540358042248798 1107 100-101 0.9209096182294694 1108 1 0.09309631614091174 1108 2 0.13526630007747542 1108 3 0.1869576065146532 1108 4 0.18647267745458862 1108 5 0.197032558282352 1108 6 0.1726632223338953 1108 7 0.2673763562552338 1108 8 0.21668980719535824 1108 9 0.2743140721545245 1108 10-11 0.308020100755364 1108 12-13 0.3310439477810547 1108 14-15 0.4471954747004574 1108 16-17 0.42168770816707735 1108 18-19 0.36184607712982597 1108 20-21 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-1.4434762770099603 2316 90-91 -1.619702112447328 2316 92-93 -1.6780465570379874 2316 94-95 -1.6187319900492767 2316 96-97 -1.7589042078103603 2316 98-99 -2.0181030489249174 2316 100-101 -1.5182540431648164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7328.0 3 1578.0 4 1701.0 5 1923.0 6 2247.0 7 2720.0 8 3459.0 9 4343.0 10 5104.0 11 6695.0 12 9212.0 13 12805.0 14 13612.0 15 15763.0 16 21071.0 17 27437.0 18 22612.0 19 14415.0 20 13674.0 21 14181.0 22 14928.0 23 16232.0 24 18083.0 25 20204.0 26 23328.0 27 27017.0 28 31615.0 29 37893.0 30 44987.0 31 54872.0 32 67733.0 33 87377.0 34 120016.0 35 178767.0 36 263815.0 37 393351.0 38 347989.0 39 50172.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.910258306653596 19.006536013257552 14.685145675124792 21.398060004964062 2 15.169589196298924 21.11163480509151 40.58800872125784 23.130767277351726 3 18.81861671409116 27.08525439680402 29.81637429771095 24.27975459139387 4 12.36794063540794 16.814523724595105 37.11439266719837 33.70314297279858 5 13.312856845802528 38.70266586831748 32.43415498162377 15.55032230425622 6 30.484668149842463 36.17530837627286 17.902165453348783 15.437858020535893 7 28.027454231267505 34.58910761233688 20.207473573975847 17.175964582419763 8 27.222729557556857 33.82943719822726 19.756238722636436 19.191594521579443 9 28.59248548546343 16.433769854223783 20.032663965697832 34.94108069461495 10-11 25.275843675776393 27.2384193965268 26.127910967090497 21.35782596060631 12-13 26.577200692768972 25.002027250247778 27.346429536785834 21.07434252019742 14-15 23.484818522658948 25.569383624360253 26.97308641394326 23.972711439037543 16-17 21.51015969979492 29.113386479009296 27.256549468397257 22.11990435279853 18-19 21.473225773828656 26.519095266560726 31.287204504242 20.720474455368613 20-21 22.968993040010176 25.618201139599428 30.88206277712508 20.530743043265314 22-23 22.151309027034404 25.88158848562017 30.789898375281545 21.177204112063883 24-25 21.61438126872509 26.11003051115578 31.18389395682754 21.09169426329159 26-27 21.417551595124916 26.40724847118368 31.026814164898564 21.148385768792842 28-29 21.224802342591484 26.337176720522166 31.342302516623157 21.095718420263196 30-31 21.35688916764628 26.25636749905794 31.15006203186598 21.2366813014298 32-33 21.140641965119215 26.47923319163598 30.864046667975465 21.51607817526934 34-35 21.52454252059269 26.504216374056288 30.748636875054093 21.222604230296934 36-37 22.01976845962485 26.169085504678403 30.476931983288104 21.334214052408644 38-39 21.516844341114854 26.39284046458144 30.584241791239634 21.506073403064068 40-41 21.495366753494014 26.384099549528745 30.666614115033852 21.453919581943392 42-43 21.608284617105642 26.40883664367644 30.102874711513135 21.880004027704782 44-45 21.492029986145987 26.837213939193504 29.867619698365665 21.803136376294844 46-47 21.75938737882656 27.149254338537787 29.243022905992866 21.848335376642787 48-49 21.9481419399593 27.01113296441957 29.031451936772136 22.009273158848995 50-51 21.957289010393463 27.396290136983964 28.68011610461872 21.966304748003857 52-53 21.98793773674088 27.46864760902073 28.44027411586182 22.10314053837657 54-55 21.45915084255083 27.50100379899311 28.543121672993298 22.496723685462754 56-57 21.70790688708083 27.794790724853623 28.28488910598525 22.212413282080295 58-59 21.79589761248737 27.172180706641093 28.58466978255228 22.44725189831926 60-61 21.834756236611284 27.37480930604082 28.87878600105015 21.91164845629775 62-63 21.79434825630748 27.334584030257826 28.81321921351757 22.057848499917124 64-65 21.524559841579386 27.14824055924386 28.89285135761294 22.434348241563814 66-67 21.62793805095912 27.487086414903455 28.445251070836726 22.439724463300703 68-69 21.734316267956526 27.343127512557135 28.643151325645487 22.27940489384085 70-71 21.622090026694746 27.47968758006163 28.53361257154684 22.36460982169679 72-73 21.712910813156068 27.480613876132242 28.26570323125087 22.54077207946082 74-75 21.53363064709446 27.812357777159352 28.088314042097455 22.56569753364873 76-77 21.62678628062155 27.527724089718504 28.16832300990843 22.67716661975152 78-79 21.68748236206254 27.463335686732155 28.287435361039165 22.56174659016614 80-81 21.61302843404614 27.9229292585918 28.11677607215791 22.347266235204145 82-83 21.790023080677916 27.700353943903306 27.86979303722839 22.63982993819039 84-85 21.771549889577884 27.364495931519457 27.833855834794957 23.030098344107703 86-87 21.748600344314294 27.277742511565865 27.937745203404575 23.035911940715266 88-89 21.725039118796836 27.147628852206413 28.09725318368369 23.030078845313064 90-91 21.59598529650007 27.354613304733427 28.085970515137397 22.96343088362911 92-93 21.842643186943413 26.982558498782023 28.086071665937407 23.088726648337158 94-95 21.658767986786078 26.877221660702048 28.174425357223953 23.289584995287917 96-97 21.813065670953506 26.777883995665775 28.090057252167238 23.31899308121348 98-99 21.866726477357123 26.714079866862612 27.906032248042234 23.513161407738036 100-101 21.989230476000106 26.190967069906478 28.476307976343513 23.343494477749907 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4484.0 1 2794.5 2 1504.0 3 1845.0 4 1491.0 5 1370.0 6 2264.0 7 4504.0 8 7006.0 9 7504.0 10 6140.0 11 5031.5 12 4948.0 13 5191.0 14 5361.5 15 5492.0 16 5577.0 17 5667.0 18 5742.5 19 5758.5 20 5987.5 21 6629.0 22 7398.0 23 8604.5 24 9950.5 25 11114.0 26 12950.5 27 15227.0 28 17422.5 29 20420.5 30 24157.0 31 27485.5 32 31183.0 33 35032.0 34 38232.5 35 40224.0 36 44101.5 37 49439.0 38 51533.0 39 52032.0 40 52781.0 41 56291.5 42 59543.0 43 61302.5 44 64160.0 45 67884.0 46 68781.5 47 68980.5 48 75394.0 49 76340.5 50 70199.0 51 66127.0 52 64119.5 53 63987.0 54 63492.0 55 60328.0 56 53848.5 57 48482.5 58 44741.0 59 38949.0 60 33588.0 61 29870.0 62 26163.0 63 20306.0 64 14924.5 65 11743.0 66 9134.5 67 7714.0 68 5903.5 69 3885.0 70 2946.0 71 2445.0 72 2103.5 73 1844.5 74 1494.0 75 1241.0 76 946.0 77 566.5 78 336.5 79 229.0 80 153.0 81 114.5 82 93.0 83 74.0 84 49.0 85 27.5 86 19.0 87 12.0 88 8.0 89 6.0 90 4.5 91 5.5 92 5.5 93 4.5 94 4.5 95 4.5 96 2.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2962114925059742 2 0.18792556967594212 3 0.18762560867087277 4 0.2093727815384 5 0.2237209162808835 6 0.11473508443902293 7 0.13038305020347357 8 0.1447811784468019 9 0.14758081449411578 10-11 0.13530741003669522 12-13 0.12398388209532761 14-15 0.13963184785977822 16-17 0.11461010068691069 18-19 0.14268145141131652 20-21 0.1708777858878346 22-23 0.1726025616669833 24-25 0.19702438682971213 26-27 0.17675202223710917 28-29 0.1748272724545809 30-31 0.16510353654024976 32-33 0.17577714897063382 34-35 0.3568286122804036 36-37 0.4730635017447732 38-39 0.4394428724265846 40-41 0.42834431523901895 42-43 0.4525161728975233 44-45 0.4569655944727185 46-47 0.4603901492805935 48-49 0.4729635147430834 50-51 0.46478957735494386 52-53 0.47108875846139997 54-55 0.4536660234169558 56-57 0.41559597252357194 58-59 0.40509733734614506 60-61 0.3604781378420805 62-63 0.46843910291662083 64-65 0.46781418415605974 66-67 0.47628808254926863 68-69 0.48783658124443824 70-71 0.48023756911601495 72-73 0.4733134692489976 74-75 0.4772379590653215 76-77 0.4537660104186456 78-79 0.4406427164468619 80-81 0.4368932038834952 82-83 0.4491416115904932 84-85 0.45181626388569485 86-87 0.4527411436513253 88-89 0.46041514603101596 90-91 0.47881275434193554 92-93 0.48308719866417366 94-95 0.4815374001379821 96-97 0.47958765360503136 98-99 0.47666303380560526 100-101 0.4588653475048244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2000260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.496059369912814 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19984068058163 27.618247972194283 2 14.558980416294997 11.791626706073604 3 6.199118882768608 7.531196589532353 4 3.335790302608973 5.403454485601296 5 1.9317186877152397 3.9113497331843203 6 1.2560260824703366 3.0518464083526657 7 0.8449683217649137 2.3952521126721273 8 0.6066245348519315 1.9652722542887648 9 0.4745433721581487 1.7295422915263516 >10 2.2974967321328448 16.79091661114524 >50 0.18042941345872265 5.0481912998206635 >100 0.10688223109512357 8.153914332599081 >500 0.005219251936762301 1.395171593980068 >1k 0.001988286452099924 1.350685528149799 >5k 1.2426790325624525E-4 0.4403315339534553 >10k+ 2.485358065124905E-4 1.4230005469259264 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16844 0.8420905282313299 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11433 0.5715756951596292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8750 0.437443132392789 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3544 0.17717696699429075 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2430 0.1214842070530831 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.1205343305370302 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.9993500844890166E-5 0.0 8 0.0 0.0 0.0 4.9993500844890166E-5 0.0 9 0.0 0.0 0.0 9.998700168978033E-5 0.0 10-11 0.0 0.0 0.0 9.998700168978033E-5 0.0 12-13 0.0 0.0 0.0 1.7497725295711558E-4 0.0 14-15 0.0 0.0 0.0 1.9997400337956066E-4 0.0 16-17 0.0 0.0 0.0 1.9997400337956066E-4 0.0 18-19 0.0 0.0 0.0 1.9997400337956066E-4 0.0 20-21 0.0 0.0 0.0 2.9996100506934095E-4 0.0 22-23 0.0 0.0 0.0 6.499155109835721E-4 0.0 24-25 0.0 0.0 0.0 0.001549798526191595 0.0 26-27 0.0 0.0 0.0 0.0028246327977362942 0.0 28-29 0.0 0.0 0.0 0.0057992460980072595 0.0 30-31 0.0 0.0 0.0 0.016022917020787297 0.0 32-33 0.0 0.0 0.0 0.033420655314809075 0.0 34-35 0.0 0.0 0.0 0.05916730824992751 0.0 36-37 0.0 0.0 0.0 0.09776229090218272 0.0 38-39 0.0 0.0 0.0 0.15852939117914672 0.0 40-41 0.0 0.0 0.0 0.23236979192704948 0.0 42-43 0.0 0.0 0.0 0.3052103226580545 0.0 44-45 0.0 0.0 0.0 0.38894943657324543 0.0 46-47 2.4996750422445083E-5 0.0 0.0 0.49251097357343543 0.0 48-49 4.9993500844890166E-5 0.0 0.0 0.6118704568406107 0.0 50-51 4.9993500844890166E-5 0.0 0.0 0.7521272234609501 0.0 52-53 4.9993500844890166E-5 0.0 0.0 0.8881345425094738 0.0 54-55 4.9993500844890166E-5 0.0 0.0 1.0274664293641826 0.0 56-57 4.9993500844890166E-5 0.0 0.0 1.2018937538120045 0.0 58-59 4.9993500844890166E-5 0.0 0.0 1.3956435663363762 0.0 60-61 4.9993500844890166E-5 0.0 0.0 1.6189645346105006 0.0 62-63 4.9993500844890166E-5 0.0 0.0 1.8441852559167309 0.0 64-65 4.9993500844890166E-5 0.0 0.0 2.0706808114945057 0.0 66-67 4.9993500844890166E-5 0.0 0.0 2.3320968274124363 0.0 68-69 4.9993500844890166E-5 0.0 0.0 2.631032965714457 0.0 70-71 4.9993500844890166E-5 0.0 0.0 2.9732884724985755 0.0 72-73 4.9993500844890166E-5 0.0 0.0 3.3305420295361605 0.0 74-75 4.9993500844890166E-5 0.0 0.0 3.6883705118334618 0.0 76-77 4.9993500844890166E-5 0.0 0.0 4.065546478957735 0.0 78-79 4.9993500844890166E-5 0.0 0.0 4.490216271884655 0.0 80-81 4.9993500844890166E-5 0.0 0.0 4.96912901322828 0.0 82-83 4.9993500844890166E-5 0.0 0.0 5.468439102916621 0.0 84-85 4.9993500844890166E-5 0.0 0.0 5.97632307799986 0.0 86-87 4.9993500844890166E-5 0.0 0.0 6.496730425044744 0.0 88-89 4.9993500844890166E-5 0.0 0.0 7.055757751492306 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9675 0.0 38.94507 1 GTATCAA 15540 0.0 37.164005 1 ATCAACG 20225 0.0 28.415781 3 TATCAAC 20305 0.0 28.397236 2 TCAACGC 20580 0.0 27.994736 4 CAACGCA 20940 0.0 27.558744 5 AACGCAG 21425 0.0 26.983036 6 TACATGG 16420 0.0 24.546595 2 GTACATG 16665 0.0 24.51776 1 ACGCAGA 23765 0.0 24.206537 7 CGCAGAG 24015 0.0 23.915678 8 GAGTACT 12000 0.0 23.455872 12-13 AGTACTT 12275 0.0 22.988289 12-13 ACATGGG 16910 0.0 22.74169 3 GCAGAGT 24880 0.0 22.645004 9 GTACTTT 12610 0.0 22.099894 14-15 CATGGGG 5165 0.0 21.391 4 TACTTTT 13660 0.0 20.349102 14-15 AGTATCG 310 2.6557245E-10 19.88507 5 CATGGGA 11220 0.0 19.863258 4 >>END_MODULE