FastQCFastQC Report
Fri 27 May 2016
SRR939357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000260
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170540.8525891634087568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125610.6279683641126654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98480.49233599632047836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38970.19482467279253698No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27910.13953186085808844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.13303270574825274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92400.042.1769331
GTATCAA157650.039.5403371
ATCAACG205500.030.2285163
TATCAAC205600.030.2138142
TCAACGC209300.029.6796934
CAACGCA212200.029.2740825
GTACGAC650.00587176229.2035483
AACGCAG217400.028.5738746
GTACATG165000.026.5606521
TACATGG163600.026.3095232
ACGCAGA245950.025.199127
CGCAGAG247250.025.0090478
ACATGGG167450.024.769393
GCAGAGT259050.023.5950689
GAGTACT130700.023.54751812-13
AGTACTT134250.022.81879212-13
GTATAGG4300.022.13221
GTACTTT139900.021.9650814-15
CATGGGA106500.021.9232274
CATGGGG55450.021.5669144
Produced by FastQC (version 0.11.5)