##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000260 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28688870446842 33.0 31.0 34.0 31.0 34.0 2 32.475309209802724 34.0 31.0 34.0 31.0 34.0 3 32.550088988431504 34.0 31.0 34.0 31.0 34.0 4 36.10255666763321 37.0 35.0 37.0 35.0 37.0 5 36.05660464139662 37.0 35.0 37.0 35.0 37.0 6 36.101542799436075 37.0 35.0 37.0 35.0 37.0 7 36.03717816683831 37.0 35.0 37.0 35.0 37.0 8 36.01435913331267 37.0 35.0 37.0 35.0 37.0 9 37.69831021967144 39.0 37.0 39.0 35.0 39.0 10-11 37.62453031105956 39.0 37.0 39.0 35.0 39.0 12-13 37.545099137112175 39.0 37.0 39.0 35.0 39.0 14-15 38.77557892473978 40.0 38.0 41.0 34.5 41.0 16-17 38.79300715906932 40.0 38.0 41.0 34.5 41.0 18-19 38.943104646395966 40.0 38.0 41.0 35.0 41.0 20-21 38.935617369741934 40.0 38.0 41.0 35.0 41.0 22-23 38.84792027036485 40.0 38.0 41.0 35.0 41.0 24-25 38.765385499885014 40.0 38.0 41.0 34.0 41.0 26-27 38.5878220831292 40.0 38.0 41.0 34.0 41.0 28-29 38.40095387599612 40.0 38.0 41.0 34.0 41.0 30-31 38.12337621109256 40.0 37.5 41.0 33.5 41.0 32-33 37.965508733864596 40.0 37.0 41.0 33.0 41.0 34-35 37.82570090888184 40.0 37.0 41.0 33.0 41.0 36-37 37.62461380020598 40.0 36.5 41.0 33.0 41.0 38-39 37.44807874976253 40.0 36.0 41.0 33.0 41.0 40-41 37.1990061292032 40.0 36.0 41.0 32.0 41.0 42-43 36.930516282883225 40.0 35.5 41.0 31.0 41.0 44-45 36.54854368932038 40.0 35.0 41.0 30.5 41.0 46-47 36.06609640746703 39.0 35.0 41.0 29.5 41.0 48-49 35.61277908871847 39.0 35.0 41.0 27.5 41.0 50-51 35.304361183046204 39.0 35.0 41.0 26.5 41.0 52-53 35.01837461130053 39.0 35.0 40.0 26.0 41.0 54-55 34.743535840340755 38.0 34.5 40.0 25.0 41.0 56-57 34.42900272964515 38.0 34.0 40.0 24.0 41.0 58-59 34.103338066051414 38.0 34.0 40.0 23.0 41.0 60-61 33.754897863277776 37.0 33.5 40.0 22.5 41.0 62-63 33.39707663003809 37.0 33.0 40.0 22.0 41.0 64-65 33.094894413726216 36.0 33.0 39.0 20.5 41.0 66-67 32.681907352044234 36.0 32.5 39.0 19.5 40.0 68-69 32.2303193084899 35.0 32.0 38.5 18.0 40.0 70-71 31.7593017907672 35.0 31.5 38.0 14.0 40.0 72-73 31.28513418255627 35.0 31.0 37.0 9.5 39.5 74-75 30.775283713117297 35.0 31.0 36.5 7.0 39.0 76-77 28.864952556167697 32.5 28.0 35.0 2.0 37.0 78-79 29.796520452341195 34.0 29.5 35.0 2.0 37.5 80-81 29.807887224660796 34.0 30.0 35.0 2.0 37.0 82-83 29.464039174907263 34.0 30.0 35.0 2.0 37.0 84-85 29.028824502814636 34.0 29.0 35.0 2.0 36.0 86-87 28.428089098417203 34.0 29.0 35.0 2.0 36.0 88-89 27.85435043444352 33.5 27.0 35.0 2.0 35.0 90-91 27.376511303530542 33.0 26.5 35.0 2.0 35.0 92-93 27.01906977092978 33.0 26.0 35.0 2.0 35.0 94-95 26.501837511123554 33.0 25.0 35.0 2.0 35.0 96-97 25.7900652915121 32.0 23.5 34.0 2.0 35.0 98-99 24.818949536560247 32.0 11.0 34.0 2.0 35.0 100-101 20.662697349344583 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20942795508028667 1101 2 0.24355846292257155 1101 3 0.2714609585448926 1101 4 0.13282796069356806 1101 5 0.16317849849469468 1101 6 0.2389134033510274 1101 7 0.22674471841997956 1101 8 0.21379177588764264 1101 9 0.22388879956823615 1101 10-11 0.2842551724029718 1101 12-13 0.2435955743874061 1101 14-15 0.28972752616857633 1101 16-17 0.31044142365145433 1101 18-19 0.34052772597765824 1101 20-21 0.3424821347315614 1101 22-23 0.33324132783130267 1101 24-25 0.2758523614604016 1101 26-27 0.24888112572504184 1101 28-29 0.2733427769068797 1101 30-31 0.30717521882249343 1101 32-33 0.42293796683895124 1101 34-35 0.4147779879976099 1101 36-37 0.3822879139590043 1101 38-39 0.43119891695170054 1101 40-41 0.4212473144460489 1101 42-43 0.377342914591992 1101 44-45 0.32225365410428 1101 46-47 0.36829651614483794 1101 48-49 0.40443688315549764 1101 50-51 0.3034955302310536 1101 52-53 0.27314959039163966 1101 54-55 0.3628417314079613 1101 56-57 0.3325440020603665 1101 58-59 0.32355686758967295 1101 60-61 0.24540074163695635 1101 62-63 0.29641833826062225 1101 64-65 0.3131429576904736 1101 66-67 0.13621844492115542 1101 68-69 0.1155568896525665 1101 70-71 0.06372505680293017 1101 72-73 0.18830531211732904 1101 74-75 0.10552757444900251 1101 76-77 0.26650389330792734 1101 78-79 0.36812881130613206 1101 80-81 0.5905505874057084 1101 82-83 0.4658819829643477 1101 84-85 0.4299105726898027 1101 86-87 0.4769640242118456 1101 88-89 0.6739367023734495 1101 90-91 0.7613115303004925 1101 92-93 0.8116972636842874 1101 94-95 0.5043550536632004 1101 96-97 0.42836798010303667 1101 98-99 0.48025363521414377 1101 100-101 0.4202892292545535 1102 1 0.29629231788643295 1102 2 0.31752587678725064 1102 3 0.24891270200691196 1102 4 0.18170716368236128 1102 5 0.21043617802976655 1102 6 0.20041973377858824 1102 7 0.23460067524854367 1102 8 0.2053235318029607 1102 9 0.31621445336649145 1102 10-11 0.23315902254825716 1102 12-13 0.30895334732223745 1102 14-15 0.3463978250477808 1102 16-17 0.33284309446921867 1102 18-19 0.2910946105770833 1102 20-21 0.3592927926809111 1102 22-23 0.40531091064575264 1102 24-25 0.37017313771611526 1102 26-27 0.3458637948823693 1102 28-29 0.3536951015229093 1102 30-31 0.376970777145452 1102 32-33 0.42276284230595707 1102 34-35 0.4860238368975658 1102 36-37 0.5090923452583027 1102 38-39 0.6762383524020947 1102 40-41 0.6424360857204405 1102 42-43 0.6169573811756379 1102 44-45 0.6887944905509329 1102 46-47 0.7975630686562525 1102 48-49 0.8796223854386085 1102 50-51 0.8870688490355434 1102 52-53 0.8402975252193698 1102 54-55 0.7865561876138472 1102 56-57 0.6785861876145347 1102 58-59 0.8003496239200985 1102 60-61 0.808462437361321 1102 62-63 0.8427199675673904 1102 64-65 0.6888512391350545 1102 66-67 0.7918600493213219 1102 68-69 0.8208235978302341 1102 70-71 0.6415542909249723 1102 72-73 0.7566765242385429 1102 74-75 0.6416667919666459 1102 76-77 0.7310768488911563 1102 78-79 0.8248133795917276 1102 80-81 0.9660798526610002 1102 82-83 0.8736782677298116 1102 84-85 0.8745323036985191 1102 86-87 0.9641150723646064 1102 88-89 0.9906059634776412 1102 90-91 1.0039627860082554 1102 92-93 0.9025739241243045 1102 94-95 0.9259835820982367 1102 96-97 0.9567225878240784 1102 98-99 0.667928759747145 1102 100-101 0.43236763315658777 1103 1 0.16073649607228901 1103 2 0.17954156642502284 1103 3 0.1955559519312402 1103 4 0.09158003221227773 1103 5 0.12687687523489188 1103 6 0.1788799042961955 1103 7 0.1614925126009794 1103 8 0.19608884307326946 1103 9 0.216165265595329 1103 10-11 0.1505522598603406 1103 12-13 0.1656602260428528 1103 14-15 0.2557365648318566 1103 16-17 0.15494385372661412 1103 18-19 0.18534500155680433 1103 20-21 0.31385685029366783 1103 22-23 0.21483978008375004 1103 24-25 0.23279106224442359 1103 26-27 0.27381695298951314 1103 28-29 0.3128131269492016 1103 30-31 0.35746719526672877 1103 32-33 0.3162918126419427 1103 34-35 0.2623525373314237 1103 36-37 0.26434881534203925 1103 38-39 0.28347904456231987 1103 40-41 0.5014090702830458 1103 42-43 0.5192042533378824 1103 44-45 0.4414679720361576 1103 46-47 0.36305627065402746 1103 48-49 0.3918345108548067 1103 50-51 0.4437183420231321 1103 52-53 0.2425598473866586 1103 54-55 0.2999166583029833 1103 56-57 0.4574546796940382 1103 58-59 0.2871918951339012 1103 60-61 0.31077457791222685 1103 62-63 0.41403372248616677 1103 64-65 0.3042867400935876 1103 66-67 0.18244910441163142 1103 68-69 0.11323102392245943 1103 70-71 0.20779918296412347 1103 72-73 0.4166395703489201 1103 74-75 0.2749401120150772 1103 76-77 0.3689192714519507 1103 78-79 0.4533980960683728 1103 80-81 0.44970536976321185 1103 82-83 0.42558460916861307 1103 84-85 0.3997851792912961 1103 86-87 0.4586019430133845 1103 88-89 0.6530498389695225 1103 90-91 0.7061166428340044 1103 92-93 0.43622182796852016 1103 94-95 0.3205530629780675 1103 96-97 0.26072130137467653 1103 98-99 0.05055128774634099 1103 100-101 -0.019576229723039518 1104 1 -0.0037906059464916098 1104 2 0.04828140504277911 1104 3 -0.033344669000456406 1104 4 0.035441807417001314 1104 5 -0.06837524703165343 1104 6 -0.02036957826072694 1104 7 -0.057917752269887046 1104 8 -0.025843544364114734 1104 9 0.01580290545494023 1104 10-11 -0.01710049833626215 1104 12-13 0.06696778221166966 1104 14-15 0.0763149012148503 1104 16-17 0.05874143599255888 1104 18-19 -0.09198739187991833 1104 20-21 -0.023767711004587966 1104 22-23 0.11469819565803618 1104 24-25 0.00608529987827211 1104 26-27 0.15081158358015756 1104 28-29 0.11180013837793723 1104 30-31 -0.11084249066782093 1104 32-33 -0.016535376367265542 1104 34-35 -0.05295957833585163 1104 36-37 0.020141178182136343 1104 38-39 -0.05776041909667384 1104 40-41 0.02054726508161764 1104 42-43 0.01218772515598232 1104 44-45 0.03387280750424537 1104 46-47 -0.020021390803208305 1104 48-49 0.1124095476007696 1104 50-51 0.02604458613627969 1104 52-53 0.18772227951912157 1104 54-55 0.12610317778584346 1104 56-57 0.08945995911330584 1104 58-59 0.08296326028373358 1104 60-61 0.040662990186874026 1104 62-63 0.13284435331677713 1104 64-65 0.13666757820138997 1104 66-67 0.05642546701173501 1104 68-69 0.07416512117175245 1104 70-71 -0.024383976888294967 1104 72-73 0.07395846527047922 1104 74-75 0.10980407697892858 1104 76-77 -0.018121554057245248 1104 78-79 0.17934194224529065 1104 80-81 0.2966511057322556 1104 82-83 0.13329436109590276 1104 84-85 -0.06283563241941437 1104 86-87 -0.019399976775439143 1104 88-89 0.06576873989041587 1104 90-91 -0.009804105883635117 1104 92-93 -0.15665365572827383 1104 94-95 -0.4933822041425522 1104 96-97 -0.22929399694250563 1104 98-99 -0.028110859417473932 1104 100-101 0.010511060675014505 1105 1 0.14721935137925612 1105 2 0.03954740788772426 1105 3 0.03794978334662602 1105 4 0.019936828754133273 1105 5 0.09486799621158326 1105 6 0.10425346583600259 1105 7 0.16031837859782172 1105 8 0.08998860357329619 1105 9 0.0944800036057245 1105 10-11 0.16112852015591983 1105 12-13 0.13348983057582586 1105 14-15 0.1599564374879705 1105 16-17 0.18841426671801997 1105 18-19 0.18879496942876273 1105 20-21 0.20214267306369038 1105 22-23 0.13183902069359732 1105 24-25 0.13208103245295177 1105 26-27 0.22519991928427885 1105 28-29 0.2340120871688427 1105 30-31 0.23048752355693125 1105 32-33 0.23230530642079117 1105 34-35 0.34847000914636794 1105 36-37 0.2686049050669155 1105 38-39 0.484479979196351 1105 40-41 0.46211910007450996 1105 42-43 0.4531834577306668 1105 44-45 0.4474218828954335 1105 46-47 0.1968064897088766 1105 48-49 0.17498422754387377 1105 50-51 0.11265198300682755 1105 52-53 0.26437946301840753 1105 54-55 0.35502209670475793 1105 56-57 0.3659037713465878 1105 58-59 0.32006852344163406 1105 60-61 0.4420001167871632 1105 62-63 0.4518557331176254 1105 64-65 0.3958069807618543 1105 66-67 0.43842404453663164 1105 68-69 0.5522590045287998 1105 70-71 0.5908934057575124 1105 72-73 0.4753667156260981 1105 74-75 0.5201027967513348 1105 76-77 0.6041330689868509 1105 78-79 0.600181202558236 1105 80-81 0.5973836804121966 1105 82-83 0.5748804208398575 1105 84-85 0.6391913946076109 1105 86-87 0.7009840915033649 1105 88-89 0.7567004610852983 1105 90-91 0.8820877860082561 1105 92-93 0.5014046657510995 1105 94-95 0.6985523620025411 1105 96-97 0.5548027313647488 1105 98-99 0.8619389272112592 1105 100-101 0.9083275613862547 1106 1 0.039967225193890954 1106 2 0.022326529155016317 1106 3 0.06000559401423544 1106 4 -0.021852699273473775 1106 5 -0.08474996971510507 1106 6 0.019725302371071507 1106 7 0.02423436370773402 1106 8 -0.008734054905630728 1106 9 -0.05313540818135465 1106 10-11 0.1217354000298414 1106 12-13 0.13537350853685837 1106 14-15 0.06046509167376968 1106 16-17 0.04440399581618948 1106 18-19 0.09098446485032241 1106 20-21 0.08909740501488983 1106 22-23 0.09584820472750266 1106 24-25 0.07401606492313562 1106 26-27 0.002627710496952318 1106 28-29 0.055203701982854625 1106 30-31 0.012062531029151558 1106 32-33 -0.038085558454362456 1106 34-35 0.21383247959714424 1106 36-37 0.23102454477020018 1106 38-39 0.1561821502150238 1106 40-41 0.10687998913245877 1106 42-43 0.0979140503453948 1106 44-45 0.1326044767057155 1106 46-47 0.045483499735240684 1106 48-49 0.08715497449384202 1106 50-51 0.2786486408879796 1106 52-53 0.33086561903830614 1106 54-55 0.3369271044486979 1106 56-57 0.29665316677631637 1106 58-59 0.33392867071223975 1106 60-61 0.3146694643946901 1106 62-63 0.3940119432091933 1106 64-65 0.37416621778056935 1106 66-67 0.23688354512081844 1106 68-69 0.3304730088219259 1106 70-71 0.28811836024491555 1106 72-73 0.3760419323203905 1106 74-75 0.2944749946699794 1106 76-77 0.38227275868606014 1106 78-79 0.5113267613442041 1106 80-81 0.47721997093199064 1106 82-83 0.5180870887745357 1106 84-85 0.5085331602245482 1106 86-87 0.6328352992853397 1106 88-89 0.6647415175209126 1106 90-91 0.5671161804326239 1106 92-93 0.6422626121141697 1106 94-95 0.9191555736972425 1106 96-97 0.8731447143552664 1106 98-99 0.8820360660314641 1106 100-101 0.6664642621986836 1107 1 0.012860864801368166 1107 2 0.06408027620309298 1107 3 0.10502122701603867 1107 4 0.06396346160131117 1107 5 0.044305747215602764 1107 6 0.007755896611740809 1107 7 0.030356425522363395 1107 8 0.05660137043020086 1107 9 0.03201857895977156 1107 10-11 0.10334316822186196 1107 12-13 -1.8433978984688792E-4 1107 14-15 0.054633038629376074 1107 16-17 0.13363832082920624 1107 18-19 0.05485077158474638 1107 20-21 0.12616824905185098 1107 22-23 0.16018609319412747 1107 24-25 0.151925145748244 1107 26-27 0.2148374166421334 1107 28-29 0.21591443339585226 1107 30-31 0.18127435509360623 1107 32-33 0.2410872764059917 1107 34-35 0.24574225602863464 1107 36-37 0.2742427194824657 1107 38-39 0.24625021804649094 1107 40-41 0.22936334019286875 1107 42-43 0.24689303287333786 1107 44-45 0.2486372707626927 1107 46-47 0.1400203979735153 1107 48-49 0.1131690721855989 1107 50-51 0.1507200447273931 1107 52-53 0.24373742116679864 1107 54-55 0.22911848224692477 1107 56-57 0.3277886793998519 1107 58-59 0.373083742211449 1107 60-61 0.4628493030057186 1107 62-63 0.39040730783756317 1107 64-65 0.2704206987918596 1107 66-67 0.4973513326363985 1107 68-69 0.5405479497835017 1107 70-71 0.48234130260807007 1107 72-73 0.5442290068964439 1107 74-75 0.5292979986114084 1107 76-77 0.23159026619673995 1107 78-79 0.36885431606489405 1107 80-81 0.5194889435700922 1107 82-83 0.672028486787859 1107 84-85 0.5227958122782965 1107 86-87 0.53498377444566 1107 88-89 0.7349994465006446 1107 90-91 0.6912745329962071 1107 92-93 0.4742412753473779 1107 94-95 0.5992276949131323 1107 96-97 0.5840523614640958 1107 98-99 0.8865019160085552 1107 100-101 0.8335621840875866 1108 1 0.042240120084237276 1108 2 -0.008086011324643039 1108 3 0.03674362108782958 1108 4 0.04059827257346882 1108 5 0.03676609810968756 1108 6 -0.01278581067695228 1108 7 0.07643384208170545 1108 8 -0.05668945521002655 1108 9 0.04727089692293163 1108 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-1.543358819787695 2316 92-93 -1.3411797975906623 2316 94-95 -1.0967126763376491 2316 96-97 -1.1134912867293139 2316 98-99 -0.8560204760607135 2316 100-101 -0.3969557590625463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 30.0 5 76.0 6 148.0 7 370.0 8 839.0 9 1550.0 10 2634.0 11 4099.0 12 6271.0 13 8606.0 14 10978.0 15 14815.0 16 19961.0 17 28417.0 18 25870.0 19 15159.0 20 13046.0 21 13661.0 22 13879.0 23 15243.0 24 17123.0 25 19740.0 26 23234.0 27 27465.0 28 33304.0 29 40175.0 30 48654.0 31 59152.0 32 72673.0 33 93972.0 34 129858.0 35 190457.0 36 286627.0 37 433660.0 38 309982.0 39 18514.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92785691722198 19.701509749946098 15.277155191865099 21.09347814096682 2 15.586723725915629 21.596492455980723 39.798426204593405 23.018357613510243 3 18.82065331506904 27.087728595282613 30.31805865237519 23.773559437273153 4 12.632007838980932 17.42128523291972 36.71677681901353 33.22993010908582 5 13.162038934938458 38.859598252227215 32.507074080369556 15.47128873246478 6 30.394798676172098 36.65893433853599 17.470928779258696 15.475338206033214 7 27.79483667123274 34.75943127393439 20.040194774679293 17.40553728015358 8 27.150570425844638 34.26679531660884 19.4041774569306 19.17845680061592 9 28.14174157359543 16.540999670042893 19.944457220561326 35.37280153580035 10-11 25.215696959395277 27.488451501304827 25.94657694499715 21.34927459430274 12-13 26.53682521272235 25.18800055992721 27.30839990801196 20.966774319338484 14-15 23.330441999170095 25.730815956165042 27.03356613989391 23.90517590477095 16-17 21.2829997570294 29.31417798446388 27.327418382375733 22.075403876130988 18-19 21.259846849832947 26.520265625601485 31.590833986998685 20.629053537566886 20-21 22.740418745563076 25.577574915261014 31.28053353064102 20.40147280853489 22-23 21.986016817813685 25.787697599312086 31.22139121914151 21.004894363732713 24-25 21.476283083199185 26.1006569146011 31.601041864557605 20.822018137642107 26-27 21.306040867707633 26.385508076704067 31.401258537019455 20.907192518568845 28-29 21.074610300660915 26.237114175157227 31.75257216561847 20.935703358563387 30-31 21.190570225870637 26.189920310359653 31.566546348974633 21.052963114795077 32-33 20.99177106976093 26.41416615839941 31.272734544509213 21.321328227330447 34-35 21.448970832655288 26.371546121990256 31.082521115659745 21.096961929694707 36-37 21.882096427925042 26.104480417733683 30.88217942269637 21.131243731644904 38-39 21.38842386838785 26.310080863989064 30.94066849464094 21.360826772982143 40-41 21.275852733689817 26.30195138034981 31.07862490716551 21.343570978794865 42-43 21.54016247416269 26.367243401033818 30.39107734433059 21.701516780472904 44-45 21.36535922895079 26.717753984468327 30.18262099637768 21.734265790203203 46-47 21.64676297926642 27.049942319926313 29.52612351159788 21.77717118920939 48-49 21.860515109967366 26.957021676013404 29.199738533010205 21.98272468100902 50-51 21.855064305406373 27.333378494140387 28.757293741137087 22.054263459316154 52-53 21.96733023073512 27.408750566801597 28.497609559562036 22.126309642901244 54-55 21.411916450861387 27.469154009978702 28.589758331416913 22.529171207742994 56-57 21.68830071561341 27.717321204134297 28.24728207886846 22.347096001383832 58-59 21.719390165557204 27.13093342465685 28.686941153825963 22.462735255959984 60-61 21.841538490765636 27.40400430747018 28.804482048708007 21.949975153056176 62-63 21.71231623436811 27.327872983115007 28.832093980816282 22.127716801700597 64-65 21.480962845070852 27.107407813836154 28.883702339621436 22.52792700147156 66-67 21.591790230326094 27.446455469838536 28.460878159790948 22.500876140044422 68-69 21.59013673674109 27.33507463264859 28.653747655582695 22.42104097502763 70-71 21.57774489316389 27.446481957345547 28.52679151710278 22.44898163238779 72-73 21.66660834091568 27.39498865147531 28.275299211102556 22.663103796506455 74-75 21.49498065251517 27.7932368792057 27.917945667063282 22.793836801215843 76-77 21.469868684392946 27.48262382607135 28.25300471100191 22.79450277853379 78-79 21.52243436718382 27.469645150598147 28.26244327603903 22.745477206179007 80-81 21.39616489472858 27.939495805041147 28.097650403098 22.56668889713227 82-83 21.616014918060653 27.766465359503268 27.773014508113945 22.84450521432214 84-85 21.486731724875767 27.423784907961963 27.801335826342576 23.288147540819693 86-87 21.458701544126864 27.367582369322758 27.844630914217095 23.329085172333286 88-89 21.411271818646895 27.24057547610308 28.00752903110392 23.3406236741461 90-91 21.266207643315294 27.44958805703787 27.957003531655815 23.327200767991016 92-93 21.35649865517483 27.16296881405417 27.959690240268763 23.520842290502234 94-95 21.14047673802406 26.99989001429814 28.08214932058832 23.77748392708948 96-97 21.271509703738513 26.987191665083536 27.993835801345824 23.747462829832124 98-99 21.162689722353388 26.975738385813635 27.785433732690052 24.076138159142932 100-101 21.137415548225373 26.523631498799656 28.46630977865811 23.872643174316863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2424.0 1 1664.0 2 1262.5 3 1572.5 4 1293.0 5 1257.5 6 2097.5 7 4335.5 8 6667.0 9 7076.0 10 5877.5 11 4791.5 12 4788.0 13 4999.5 14 5203.0 15 5456.0 16 5541.5 17 5613.0 18 5740.0 19 5815.0 20 6071.0 21 6758.5 22 7536.5 23 8659.0 24 10028.5 25 11265.0 26 13207.5 27 15471.5 28 17481.5 29 20600.5 30 24307.0 31 27548.5 32 31261.0 33 35044.0 34 38389.5 35 40538.5 36 44521.0 37 49747.5 38 51743.5 39 52320.5 40 53026.5 41 56671.0 42 60034.0 43 62038.0 44 65375.5 45 69294.0 46 70102.0 47 69654.0 48 76201.5 49 77316.5 50 70766.0 51 66251.5 52 63495.0 53 63466.5 54 63589.5 55 60380.5 56 53475.0 57 48024.5 58 44453.0 59 38833.5 60 32985.0 61 29157.5 62 25776.0 63 19866.5 64 14357.5 65 11354.5 66 8816.5 67 7349.5 68 5744.5 69 3736.0 70 2782.0 71 2317.5 72 1991.0 73 1788.0 74 1513.5 75 1264.0 76 882.0 77 481.0 78 317.0 79 213.5 80 126.5 81 84.5 82 58.0 83 37.5 84 25.0 85 12.5 86 6.5 87 1.5 88 0.5 89 1.0 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.294461719976403 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001499805025346705 16-17 8.998830152080231E-4 18-19 4.9993500844890166E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.9993500844890166E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013248277723895893 36-37 0.006699129113215282 38-39 0.002474678291822063 40-41 0.003024606801115855 42-43 1.7497725295711558E-4 44-45 0.001224840770699809 46-47 1.249837521122254E-4 48-49 3.749512563366763E-4 50-51 2.4996750422445083E-5 52-53 4.9993500844890166E-5 54-55 0.0 56-57 8.998830152080231E-4 58-59 0.0022497075380200574 60-61 6.999090118284623E-4 62-63 0.0010998570185875836 64-65 2.4996750422445083E-5 66-67 4.4994150760401154E-4 68-69 0.0014748082749242598 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2571165748452701 78-79 1.499805025346705E-4 80-81 9.998700168978033E-5 82-83 0.0 84-85 0.0 86-87 0.10701108855848739 88-89 0.37197664303640526 90-91 0.27221461210042697 92-93 0.0 94-95 0.0 96-97 0.0 98-99 7.499025126733525E-5 100-101 2.99961005069341E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2000260.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.853100717033676 #Duplication Level Percentage of deduplicated Percentage of total 1 66.39865288710949 25.797935480982236 2 15.248297526937202 11.848872791547732 3 6.629106834498175 7.726840665142016 4 3.4976582066214115 5.4357946630248435 5 2.0737383054156964 4.02855816205434 6 1.3156632715617784 3.067055855981508 7 0.9075014119804924 2.468147063236984 8 0.6770855418991825 2.104549820276501 9 0.4858345508199626 1.6988560861340545 >10 2.444300394624381 17.049950843200808 >50 0.1960098509344719 5.251657296099201 >100 0.11854160144417092 8.744712868460946 >500 0.005159061799125021 1.3942992297094183 >1k 0.0020636247196500084 1.4052235120583492 >5k 1.2897654497812553E-4 0.49349693840491493 >10k+ 2.5795308995625105E-4 1.4840487236861857 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17054 0.8525891634087568 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12561 0.6279683641126654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9848 0.49233599632047836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3897 0.19482467279253698 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2791 0.13953186085808844 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2661 0.13303270574825274 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.499025126733525E-5 0.0 14-15 0.0 0.0 0.0 9.998700168978033E-5 0.0 16-17 0.0 0.0 0.0 9.998700168978033E-5 0.0 18-19 0.0 0.0 0.0 1.499805025346705E-4 0.0 20-21 0.0 0.0 0.0 2.4996750422445085E-4 0.0 22-23 0.0 0.0 0.0 6.499155109835721E-4 0.0 24-25 0.0 0.0 0.0 0.001549798526191595 0.0 26-27 0.0 0.0 0.0 0.002774639296891404 0.0 28-29 0.0 0.0 0.0 0.005874236349274594 0.0 30-31 0.0 0.0 0.0 0.0162478877745893 0.0 32-33 0.0 0.0 0.0 0.03364562606861108 0.0 34-35 0.0 0.0 0.0 0.059392279003729516 0.0 36-37 0.0 0.0 0.0 0.09863717716696829 0.0 38-39 0.0 0.0 0.0 0.1601291832061832 0.0 40-41 0.0 0.0 0.0 0.2345945027146471 0.0 42-43 0.0 0.0 0.0 0.308609880715507 0.0 44-45 0.0 0.0 0.0 0.3938987931568896 0.0 46-47 0.0 0.0 0.0 0.49848519692439985 0.0 48-49 0.0 0.0 0.0 0.6184945957025587 0.0 50-51 0.0 0.0 0.0 0.7597512323397958 0.0 52-53 0.0 0.0 0.0 0.8971583694119765 0.0 54-55 0.0 0.0 0.0 1.0382900222971014 0.0 56-57 0.0 0.0 0.0 1.214392129023227 0.0 58-59 0.0 0.0 0.0 1.410691610090688 0.0 60-61 0.0 0.0 0.0 1.6363122794036775 0.0 62-63 0.0 0.0 0.0 1.8625078739763832 0.0 64-65 0.0 0.0 0.0 2.0911031565896434 0.0 66-67 0.0 0.0 0.0 2.3536690230270065 0.0 68-69 0.0 0.0 0.0 2.654079969603951 0.0 70-71 0.0 0.0 0.0 2.9977352944117266 0.0 72-73 0.0 0.0 0.0 3.3574385329907113 0.0 74-75 0.0 0.0 0.0 3.716866807315049 0.0 76-77 0.0 0.0 0.0 4.096042514473118 0.0 78-79 0.0 0.0 0.0 4.522687050683411 0.0 80-81 0.0 0.0 0.0 5.002599662043934 0.0 82-83 0.0 0.0 0.0 5.503834501514803 0.0 84-85 0.0 0.0 0.0 6.015343005409297 0.0 86-87 0.0 0.0 0.0 6.538749962504874 0.0 88-89 0.0 0.0 0.0 7.10282663253777 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9240 0.0 42.176933 1 GTATCAA 15765 0.0 39.540337 1 ATCAACG 20550 0.0 30.228516 3 TATCAAC 20560 0.0 30.213814 2 TCAACGC 20930 0.0 29.679693 4 CAACGCA 21220 0.0 29.274082 5 GTACGAC 65 0.005871762 29.203548 3 AACGCAG 21740 0.0 28.573874 6 GTACATG 16500 0.0 26.560652 1 TACATGG 16360 0.0 26.309523 2 ACGCAGA 24595 0.0 25.19912 7 CGCAGAG 24725 0.0 25.009047 8 ACATGGG 16745 0.0 24.76939 3 GCAGAGT 25905 0.0 23.595068 9 GAGTACT 13070 0.0 23.547518 12-13 AGTACTT 13425 0.0 22.818792 12-13 GTATAGG 430 0.0 22.1322 1 GTACTTT 13990 0.0 21.96508 14-15 CATGGGA 10650 0.0 21.923227 4 CATGGGG 5545 0.0 21.566914 4 >>END_MODULE