FastQCFastQC Report
Fri 27 May 2016
SRR939356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039068
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173710.8519088132421283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116150.569622984618463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92180.452069278709685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36890.18091598710783555No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25520.12515521797213236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25220.12368395757277344No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97700.039.2871
GTATCAA160350.038.3647961
ATCAACG206550.029.830813
TATCAAC206900.029.6886462
TCAACGC209800.029.3913124
GCGTATC650.005842587429.2333581
CAACGCA212550.029.0333585
AACGCAG218400.028.3413076
GTACATG171050.025.6892071
TACATGG170300.025.4573172
ACGCAGA246000.025.0844867
CGCAGAG247950.024.8298668
ACATGGG174600.023.933863
GAGTACT120350.023.74713312-13
GCAGAGT255500.023.6503729
AGTACTT123300.023.08287612-13
ATGGGAT40500.022.60395
CATGGGA111950.022.2018054
GTACTTT128800.022.0617514-15
GTATAGG4550.021.9250181
Produced by FastQC (version 0.11.5)